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Citations for Thermo Fisher :
3001 - 3050 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection was performed overnight on attached cells growing in 6-well plates using the Lipofectamine 3000 reagent (Thermo Fisher Scientific) to the proportions of 10μL of 20μM siRNA per well ...
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bioRxiv - Cancer Biology 2020Quote: A549/KZFP cells and NC cells were seeded at 1.0 × 105 cells/well in 6-well plates (Thermo Fisher Scientific). After 72 hours of seeding ...
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bioRxiv - Cancer Biology 2019Quote: ... for IL-6) for 2 hours at RT and followed by incubating in 1:1000 DAPI (62248, Thermo Fisher Scientific) in PBS for 1min ...
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bioRxiv - Neuroscience 2020Quote: ... Detection of mitochondrial superoxide was performed in acute brain slices (400 μm) from 6 month-old mice by incubation with 5μM MitoSOX Red (Life Technologies) in ACSF for 10 min ...
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bioRxiv - Plant Biology 2021Quote: ... equal amounts of RNA were prepared in equal volumes (6 μL) using UltraPure DNase/RNase-free distilled water (Invitrogen™). RNA samples were denatured at 95°C for 3 minutes and placed on ice immediately to prevent the formation of secondary structures ...
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bioRxiv - Cell Biology 2021Quote: qPCR analysis was performed using the LabTaq Green Hi Rox (Labtech) following manufacturer’s instructions on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) and primers listed in Supplementary Table S4.
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bioRxiv - Microbiology 2020Quote: 1 μg of total RNA from mock- and ZIKV-infected cells was resuspended in 2x formamide RNA loading buffer and run on a 6% denaturing PAGE gel (Invitrogen) with an RNA ladder (Thermo Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were counterstained with DAPI (4’,6-diamidino-2-phenylinodole) and mounted onto glass slides (Superfrost Plus; Fisher Scientific) with Vectashield mounting medium (H-1200 ...
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bioRxiv - Microbiology 2020Quote: ... Organoids in expansion media grew in culture for 4-6 months and needed to be routinely passaged with TRYPLE (Gibco). To induce differentiation to a hepatocyte-like fate ...
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bioRxiv - Plant Biology 2020Quote: ... qPCR reactions were performed as described in (Ramirez-Garcés et al., 2016) and conducted on a QuantStudio 6 (Applied Biosystems) device using the following conditions ...
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bioRxiv - Neuroscience 2021Quote: Hair cell nuclei were specifically labeled by briefly exposing free-swimming larvae to 4’,6-Diamidino-2-Phenylindole (DAPI; Invitrogen/ThermoFisher) diluted to 2.5 μg/ml in E3 ...
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bioRxiv - Microbiology 2021Quote: ... Streptomyces hyphae were incubated with 0.5 mg/ml FM 4-64 Dye (N-(3-Triethylammoniumpropyl)24-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Molecular Probes) for 15 min in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... embedded organoids were mechanically dissociated from Matrigel and transferred to a 6 well-plate on a CO2 resistant orbital shaker (ThermoFisher) and grown in differentiation medium consisting of DMEM:F12 supplemented with 1X N2 supplement ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Neuroscience 2019Quote: ... Neurons were transfected with the CofActor optogenetic system (6 µg plasmid/plate) on day in vitro 3-5 (DIV3-5) using Lipofectamine 2000 (Invitrogen). 48 hours post transfection ...
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bioRxiv - Neuroscience 2019Quote: ... frozen brains from C57BL/6 WT or Prnp0/0 (PrP-KO) mice 43 were gently chopped in few drops of Hibernate-E (Gibco) and transferred to a 15 mL tube containing 75 U/ml of collagenase type III (Worthington ...
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Microbiology 2021Quote: ... for 6 min and seeding cells at a density of 2×106 cells/T75 flask (Nunc; Thermo Fisher Scientific, Singapore).
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bioRxiv - Physiology 2021Quote: ... we incubated the slices for 50 minutes at RT in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03% Pluronic F-127 (w/v) ...
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bioRxiv - Plant Biology 2021Quote: The MS/MS spectra from each LC-MS/MS run were searched against the proteomes listed in Supplementary Table 6 using the SEQUEST searching engine of Proteome Discoverer (Version 2.1, PD2.1, Thermo Scientific, USA). The search criteria were set as follows ...
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bioRxiv - Microbiology 2019Quote: ... 1 nM of CAT protein (Chloramphenicol acetyltransferase, containing a 6×His tag at C-terminus) expressed in the same baculovirus-expression system (ThermoFisher) was used to coat plate wells ...
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bioRxiv - Cancer Biology 2020Quote: ... or 100 pmol (for each well in 6-well plate) of each siRNA was transfected into abl or DU145 cells using Lipofectamine RNAiMAX reagent (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... MDA-MB-231 cells in 6-well plates were transiently transfected with 4 µg of siRNA using Lipofectamine 2000 (Invitrogen). At 48h post-transfection ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... 2 µg DNA was mixed with 6 µL of Fugene HD in 100 µL of Opti-MEM (Thermo Fisher Scientific) and incubated at room temperature for 30 min ...
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bioRxiv - Neuroscience 2020Quote: ... LUHMES cells were grown to 80% confluency and passaged 1:6 using diluted trypsin-EDTA (0.25%, Thermo Fisher Scientific 25200056) with PBS at 1:1 volume ...
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bioRxiv - Genomics 2021Quote: Organoids seeded in 6-well plates were harvested after 3-days incubation with WYE-132 in RIPA buffer (Thermo Scientific) supplemented with protease inhibitors (Complete Mini ...
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bioRxiv - Cell Biology 2020Quote: HUVECs were plated in 6-well tissue culture plates and transfected with siRNA at a 50 nM concentration with RNAiMAX (ThermoFisher) using the protocol provider by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... HEK 293T cells were seeded at 5×10^6 cells and grown to 70-90% confluence in 10 cm plates in DMEM (Gibco) with 10% (v/v ...
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bioRxiv - Cell Biology 2020Quote: ... iPSCs-derived small molecule neural precursor cells (smNPCs) were cultured on Matrigel-coated 6-well plates and kept in N2B27 medium consisting of DMEM-F12 (Invitrogen)/Neurobasal (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... 1 µg of plasmid was used to transfect 400,000 cells in each well of a 6-well plate using Lipofectamine 2000 (5 µL; Thermo Fisher) in OptiMEM (400 µL ...
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bioRxiv - Cell Biology 2021Quote: ... Transcript abundance was quantified on a QuantStudio 6 Real Time PCR system using SYBR® Green PCR Master Mix (ThermoFisher) and gene-specific primers (Supplementary Table S5) ...
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bioRxiv - Developmental Biology 2022Quote: ... The 6 μm thickness section was prepared on the Microm HM 340 E rotary microtome (Thermo Scientific, Waltham, MA, USA), and dewaxed in xylene and serial concentrations of ethanol before staining ...
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bioRxiv - Biophysics 2022Quote: ... Purified Syb2 (29–96, S61C) was labeled with 6× molar excess BODIPY FL N-(2-aminoethyl)-maleimide (BDPY) (Molecular Probes) and SN25 FL (1–206 ...
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bioRxiv - Neuroscience 2022Quote: ... Cell aggregates were transferred into ultra-low attachment 6-well plates in EB medium consisting of 20% knockout serum replacement (Gibco), 79% DMEM/F-12 ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... incubated with secondary antibodies at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (4′,6-diamidino-2-phenylindole; Invitrogen).
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bioRxiv - Cell Biology 2022Quote: ... Cells were transfected with 2.5 µg of plasmid per well of a 6-well plate using Lipofectamine 3000 (Invitrogen®) and protein expression levels analyzed 24h after transfection.
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... 1µM PRMT5 inhibitor or negative control treated (3 or 6 day) OCI-AML-20 cells were incubated with 10 µM EdU (Invitrogen) for 2 h at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... 1:1 serial dilutions of UPF3B-WT were then added to the nucleotide solutions and incubated on ice for 30 mins before supplementing with 7.5% (final) glycerol and running on a Novex 6% Tris-Glycine WedgeWell gel (Invitrogen #XP0006A) equilibrated in Novex Tris-Glycine Native Running Buffer at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: 500.000 MDA-MB-468 cells were transfected with 40 pmol siRNA upon seeding in 6 well-plates using Lipofectamine 3000 (ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: In CD protocol 2 hESCs at 80%-90% confluency in 6-well plates were lifted with 1 ml/well UltraPure 0.5 M EDTA (Invitrogen, 15575038), counted ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with 4 μg plasmid using Lipofectamine 2000 transfection reagent (Invitrogen). After 24 h culture ...
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bioRxiv - Neuroscience 2022Quote: ... The EBs were fed every other day for 6 days then transferred to the low adhesion 24-well plates in the induction media containing DMEM/F12 (11330032, Gibco), 1× N2 supplement (17502048 ...
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bioRxiv - Neuroscience 2022Quote: ... spheroids were transferred to an ultra-low attachment 6-well plate placed on an orbital shaker at 65 rpm and medium was changed to Neurobasal medium (Gibco ThermoFisher) supplemented with 2% B27 supplements (Gibco ThermoFisher) ...
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bioRxiv - Physiology 2022Quote: ... The prepared slices were stained in the dye-loading solution (6 μM Oregon Green 488 BAPTA-1 AM (OGB-1, Invitrogen), 0.03 % Pluronic F-127 (w/v) ...
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bioRxiv - Immunology 2022Quote: ... were assessed using the following ELISA kits per manufacturer protocols: (IgG; Euroimmun - EI2606-9601G, IgA; Euroimmun - EI2606-9601A, IL-6; Invitrogen - 88-7066.22 ...