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Citations for Thermo Fisher :
2951 - 3000 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: ... MCF7 cells expressing either SNHG7 isoform or vector alone were created by cloning and then transfecting (Fugene 6) the respective SNHG7 isoform from MCF7 generated cDNA using the GeneRacer Kit (Thermofisher) after 3’ RACE (see Supplementary Methods for primers and additional details) ...
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bioRxiv - Developmental Biology 2019Quote: ... E8 medium was replaced daily and hiPSC were gently passaged every 5-6 days by mechanical selection or bulk passaged using non-enzymatic reagents (i.e., Versene solution (ThermoFisher Scientific) or Phosphate-Buffer-Saline (PBS)-based enzyme-free cell dissociation buffer (ThermoFisher Scientific ...
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bioRxiv - Immunology 2019Quote: ... adult females aged 6 to 11 days after eclosure were injected with fluorescent bioparticles (E. coli K-12 Strain Bioparticles TexasRed Conjugate; Invitrogen) or pH sensitive fluorescent bioparticles (pHrodo E ...
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bioRxiv - Cell Biology 2019Quote: ... FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) was purchased from Invitrogen. Rapamycin was purchased from LC Laboratories ...
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bioRxiv - Cell Biology 2019Quote: U2OS cells were seeded on 25 mm coverslips in a 6-well dish and incubated with 10 μM BrdU (Invitrogen) for 12 to 24h ...
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bioRxiv - Microbiology 2019Quote: ... at room temperature for 60 min and washed again with PBS-T and then incubated with DAPI (4’, 6-diamidino-2-phenylindole) (Invitrogen) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2019Quote: A total of 8 µg of plasmid DNA was transfected into semi-confluent HEK293FT cells on 6-cm-diamitor culture dishes using Lipofectamine 2000 (Invitrogen). Epoxomicin or DMSO was added to the culture 4 h before lysate preparation ...
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bioRxiv - Neuroscience 2019Quote: ... Larvae were placed in a 6 cm Petri-dish containing a mixture of 0.2 M sucrose and 0.08% Brilliant Blue R dye (Acros Organics, #191490050). The larvae were allowed to feed on the sugar solution for 15 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... Assays were conducted at 25 °C in 0.5 mL containing 50 mM MES (pH 6) and 80 mg/mL soluble starch (Acros Organics). Some assays solution also contained 100 mM KCl ...
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bioRxiv - Cancer Biology 2019Quote: 4T1 and 67NR cells at 70-80% confluence were transfected with the shRNA constructs described above in FuGene 6 at a ratio of 3:2 per manufacturer’s instructions (Thermo Fisher). Pools of transfected cells were selected in media with 7 µg/ml puromycin ...
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bioRxiv - Cell Biology 2019Quote: ... Nuclei were stained with 10.9 μM 4⍰6-diamidino-2-phenylindole (DAPI) and cover glasses were mounted in ProlongGold (Life Technologies). Images were collected on an inverted epifluorescence microscope (Nikon TE2000E ...
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bioRxiv - Neuroscience 2019Quote: ... proliferating NSCs were plated on matrigel-coated 6- or 12-well plates and cultured in Neurobasal medium supplemented with B27 without vitamin A (2%, ThermoFisher), PenStrep (1% ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Biochemistry 2019Quote: ... following the manufacturer’s instructions and qRT-PCR was performed with a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using PerfeCTa SYBR Green SuperMix ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
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bioRxiv - Cell Biology 2019Quote: 293T cells in 6-well plates at ∼80% confluency were transfected with 3µg plasmid encoding eYFP using TurboFect transfection reagent (Thermo Fisher). 24h post transfection ...
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Molecular Biology 2019Quote: Cells from one well of a 6-well plate were spun down and resuspended in 1mL TRIzol® (Life Technologies) for each respective sample ...
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bioRxiv - Microbiology 2019Quote: Huh7 cells seeded at 5×105 in a 6 cm cell culture dish were transfected 24 hours later with the indicated siRNA duplex using Lipofectamine 3000 (Invitrogen) as per manufacturer’s protocol ...
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bioRxiv - Bioengineering 2019Quote: ... denaturing gels were run with 6 μL per well of a 1:1 mixture of sample and Gel Loading Buffer II with formamide (Ambion) in a 10% TBE-Urea ...
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bioRxiv - Immunology 2020Quote: ... Samples were diluted 1:5 in TE buffer and 6% were mixed with TaqMan Universal PCR Master Mix (Applied Biosystems). The sample mix and TaqMan assays were loaded separately into the wells of 96.96 Gene expression Dynamic Arrays (Fluidigm ...
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bioRxiv - Cancer Biology 2019Quote: 22RV1 cells were seeded in 6-well plates and transfected in solution with 20nM miRVana mimic (miR-194 or negative control; Ambion) using RNAiMAX (Life Technoloies) ...
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bioRxiv - Bioengineering 2019Quote: ... 500 ng of each Acr vector and GFP plasmid used as a transfection control were transfected in a 6-well format using Lipofectamine 2000 (Invitrogen). The total DNA amount was kept constant by adding a stuffer plasmid in all cases.
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bioRxiv - Physiology 2020Quote: ... Measurements of mRNA were performed with SYBR green chemistry using the QuantStudio 6-flex Real-time PCR System (ThermoFisher Scientific). All data were normalized to either Hprt or Actinb by the ΔC(t ...
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bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
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bioRxiv - Molecular Biology 2019Quote: For depletion experiments HeLa cells were transfected in 6-well plates at 60-80% confluency using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher, #11668019). Puromycin (conc ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... mixtures were subjected to 8% SDS-PAGE with 6 µl of molecular weight marker (SeeBlue Plus2 Pre-Stained Standard, Invitrogen), after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane ...
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bioRxiv - Microbiology 2021Quote: Total RNA was also extracted from ovine abomasum and ileum tissue harvested at post-mortem from five individual 6-month old helminth-free Texel cross lambs and stored in RNAlater (ThermoFisher). Specifically ...
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bioRxiv - Microbiology 2021Quote: ... with a N-terminal gp67 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFastBac-Dual vector (Invitrogen). The recombinant baculoviruses were generated according to the manufacture’s instruction to infect Hi5 cells at a density of 2×106 cells/ml ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was performed by using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA) with cDNA as the template ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... The coverslips were then mounted on slides using prolong Gold mounting medium with DAPI (4’,6-diamidino-2-phenylindole) (Invitrogen). RGCs immunohistochemistry was performed as described earlier 36–39 using SOX2 ...
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bioRxiv - Cell Biology 2020Quote: ... Seoul National University) were seeded into 6-well culture plates using DMEM supplemented with 10% newborn bovine serum (Thermo Fisher), and 1% penicillin/streptomycin at a density of 1 × 105 cells/well ...
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bioRxiv - Bioengineering 2020Quote: ... The CdWO4 scintillator plates were taken out from the 6-well plates and mounted onto a standard glass coverslip (0.1 mm thick, Fisher Scientific) upside down ...
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bioRxiv - Bioengineering 2021Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (Fisher Scientific). Data acquisition was performed using a confocal microscope (Nikon A1R HD25 ...
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bioRxiv - Bioengineering 2020Quote: ... Primary fibroblast passages 2–6 were achieved in culture in 37°C in a 5% CO2 incubator with DMEM High Glucose (Gibco) with 10% FBS to 80% confluence in 100 mm Petri dishes ...
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bioRxiv - Bioengineering 2020Quote: ... A wad punch was used to create discs that fit snuggly into Nunc™ 6-well plates (Thermo Fisher Scientific). Individual microwells measured 2 mm x 2 mm with a depth of 0.8 mm [3] ...
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bioRxiv - Biochemistry 2020Quote: ... The cells were cultured in a 6-well plate with the DMEM medium (Thermo) and 10% FBS (Gibco, cat# 10091148) to generate the lentivirus ...
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bioRxiv - Neuroscience 2021Quote: ... for each sample was loaded into the wells of a 96 well plate in a volume of 6 µL of nuclease-free water containing 1 U/µL SUPERaseIN (ThermoFisher). After adding 1.5 µL of 10 µM barcoded RT primer (Integrated DNA Technologies) ...
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bioRxiv - Neuroscience 2021Quote: ... were harvested from male and female adult WT C57BL/6 mice and immersed in a 4% paraformaldehyde solution (PFA) made in phosphate-buffered saline (PBS, Cat #AAJ19943K2, ThermoFisher) and stored at 4 °C overnight prior to transfer to 15% sucrose solution in PBS for an additional 24 h ...
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bioRxiv - Neuroscience 2021Quote: ... at room temperature in a holding chamber containing oxygenated imaging media (50% MEM, 50% HBSS, 6 mg/mL D-glucose, and 25 mM HEPES; all from Invitrogen/ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... Then animals were placed in the middle of a 6 cm diameter petri dish lid and embedded in 0.8% low melting point agarose (Thermo Fisher). To bypass the blood brain barrier and permit for acute pharmacological intervention ...
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bioRxiv - Neuroscience 2021Quote: ... samples were washed 5-6 times over 2 hours in PBT at room temperature and incubated with secondary antibodies (Invitrogen) diluted in NGS-PBT for 24 hours at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... in PBS for two hours at room temperature followed by 0.5 μg/mL of 4′,6-diamidino-2-phenylindole (DAPI) (ThermoFisher Scientific) in PBS for five minutes at room temperature prior to mounting.
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bioRxiv - Neuroscience 2020Quote: ... Transfection was carried out in unsupplemented NBA using 500 ng of the luciferase reporter construct and 20 ng of a normalizer plasmid pGL4.83-mPGK-hRLuc at 5-6 DIV using Lipofectamine 2000 (Thermo Scientific) with DNA to Lipofectamine ratio of 1:2 ...
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bioRxiv - Neuroscience 2020Quote: ... After electroporation the cells were immediately plated at low density onto vitronectin-plated 6 well plates containing warm Stemflex media supplemented with ROCK inhibitor (RevitaCell, Gibco). After 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: Isolated human PBMCs were seeded and grown in 6 well culture dish with hybridoma-(SFM) serum free medium (Cat #12045076, Gibco). PBMCs were treated with patient EVs under serum free conditions for 24 hrs ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cell Biology 2021Quote: 1.5×104 cells were seeded in one well of a 6-well plate (Falcon) with growth medium comprising of DMEM/F-12 (Gibco) with 20% fetal bovine serum (FBS ...