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Citations for Thermo Fisher :
3101 - 3150 of 4593 citations for 6 Nitrochrysene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... sample aliquots containing 25 µg of dried peptides were subsequently labeled with isobaric tag (TMT 6-plex, Thermo Fisher Scientific) following a recently established protocol 45 ...
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bioRxiv - Cancer Biology 2019Quote: The human myeloma cell line KJON (19) was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... The samples were then allowed to adsorb on the grid for 30s before blotting away the excess buffer using a blot force of 6 and blot time of 6s in a FEI Vitrobot Mark IV (ThermoFisher). The grids were plunge frozen in a liquid ethane slush and stored in liquid nitrogen until imaging ...
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bioRxiv - Immunology 2019Quote: ... Transient transfections of wild-type or mutated MCM10-expressing plasmids were performed by transfecting 2×106 cells with 6 μg of DNA using Lipofectamine 2000 according to manufacturers’ instructions (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... 6-well plate format) infected at multiplicity of infection (MOI) of 0.01 were extracted using TRIzol reagent (Thermo Fisher Scientific), according to the manufacturer’s specifications ...
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bioRxiv - Evolutionary Biology 2019Quote: ... PCR was conducted using a 5’ fluorescently-labelled primer (6-FAM, PET, NED, or VIC) (Applied Biosystems, Foster City, CA) and an unlabeled reverse primer ...
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bioRxiv - Cell Biology 2019Quote: ... Two x 106 cells/well of a 6-well plate were seeded in DMEM containing 10% FCS (Invitrogen, Carlsbad; USA), 1% HEPES (Carl Roth ...
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bioRxiv - Biochemistry 2020Quote: The C-terminal fragments of Stu2 used in the pulldown experiments were cloned into a pLIC vectors with a TEV-cleavable N-terminal 6-His tag and transformed into Bl-21 AI cells (Invitrogen). Cells were grown to an OD600 of 0.6 in TB media with 0.1% glucose and were induced by addition of IPTG and arabinose to 200 μM and 0.2% ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were seeded onto 6-well cell culture plates and transiently transfected with different GIPR constructs using Lipofectamine 2000 transfection reagent (Invitrogen). All the mutant constructs were modified by single-point mutation in the setting of the WT construct (HA-Flag-3GSA-GIPR(22-466)) ...
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bioRxiv - Cell Biology 2021Quote: ... They were washed in PBST for 6 × 10 min before being incubated with secondary antibody (1:400 in PBST) (ThermoFisher) for 30 min at room temperature ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... HUVEC seeded at 1.5 × 106 cells per well of a collagen-coated 6-well pate were transfected with plasmids using the Neon Transfection System (Invitrogen), harvested 48 hours post-transfection ...
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bioRxiv - Neuroscience 2021Quote: ... Longitudinal imaging of vascular leakage in C57BL/6 mouse was performed for 5 days after injecting 600 μg of 3kDa TRITC-dextran (LIF-D-3308, Invitrogen) and 600 μg of 2MDa FITC-dextran (LIF-D-7137 ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated on coverslips coated with poly-L-lysine in 6-well plates and cultured in Neurobasal medium (Gibco) supplemented with B27 (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA was extracted from 1 confluent well of a 6-well plate using RNA PureLink RNA mini kit (Life Technologies) as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... 300,000 cells in 6-well plate were transfected for 48 h with 2 µg of plasmid DNA using Lipofectamine 2 000 (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Pathology 2021Quote: ... The mice were tail vein-injected with 100 μL of 6 mg/mL Hoechst dye (Thermo Fisher Scientific, Waltham, MA) 4-6 h prior to termination of an experiment to visualize nuclei (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... 200 ng of nLYN-mCherry and 500 ng of iLID plasmid were mixed with 2.1 ul (1 to 3 ratio) of Fugene 6 in 125 ul of Opti-MEM medium (Thermofisher Scientific). After 10 minutes incubation at room temperature ...
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bioRxiv - Genomics 2020Quote: ... cells were harvested and grown for 5-6 passages in NPC expansion medium (selection medium with the addition of 2% B27 [GIBCO] ...
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bioRxiv - Microbiology 2020Quote: ... and RNA integrity was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... and the efficiency of rRNA depletion was verified by running 50 ng of total RNA on a Novex 6% TBE-urea polyacrylamide gel (Invitrogen).
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bioRxiv - Microbiology 2021Quote: ... Cells were washed three times with DPBS and nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (300 nM DAPI stain solution in PBS; Invitrogen) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Assays were performed in analytical triplicates using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Warrington, Cheshire, UK) and target gene expression levels were normalized to average expression of housekeeping gene GAPDH using ΔΔCT method ...
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bioRxiv - Microbiology 2021Quote: ... The freeze-dried material was dissolved in 300 μl of 6:1 (v/v) of methyl sulphoxide D6 (99.9% atom D + 1% tetramethylsilane, ACROS Organics):D2O (100% atom D ...
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bioRxiv - Microbiology 2021Quote: ... HeLa cells were transfected with 0.25 μg/6 × 104 Hela cells of the myc-Rac1Q61L plasmid with turbofect (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: Neural retinae were dissected from adult zebrafish dark adapted for 12 hours between 5:30 PM and 6:30 PM and stored in RNAlater (Ambion) at 4°C until extraction ...
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bioRxiv - Biochemistry 2021Quote: ... were seeded in 6-well plate with a cover glass inside and were transfected using lipofectamine 2000 (Thermo Fisher, #11668027) with mouse mEGFP M-Lip or mEGFP M-Lipxtal and mApple-Sec61b ...
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bioRxiv - Biochemistry 2021Quote: ... we used the same amount of chromatin proteins for different conditions and labeled them with different amine-reactive TMT 6-plex reagents (ThermoFisher). Then we mixed these samples together and carried out mass spectrometry analysis ...
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bioRxiv - Cancer Biology 2020Quote: ... MEFs were seeded at a density of 200 000 cells per well of a 6-well plate in MEF media consisting of DMEM 1x (41965-039, Invitrogen) supplemented with 10% fetal calf serum ...
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bioRxiv - Cancer Biology 2020Quote: Cells were grown in 6-well culture dishes to 70-80% confluency and RNA was isolated with TRIzol reagent (#15596-026, Ambion), DNase I treated using the RNase free DNase kit (#79254 ...
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bioRxiv - Microbiology 2021Quote: ... volvulus worm to each well of 6-well plates containing 5 ml of DMEM +10% fetal bovine serum (Gibco, A3840002) + 100 u/ml penicillin + 100 ug/ml streptomycin + sodium bicarbonate (2 g/1) ...
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bioRxiv - Neuroscience 2019Quote: ... Perkin-Elmer Life Sciences) for 15 min and then stained with 4’,6-diamidino-2-phenylindole (DAPI) before mounting in Prolong Diamond (Invitrogen).
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bioRxiv - Cell Biology 2019Quote: ... De-epidermized dermis were then placed at the bottom of a 6-well plate in 3D-HRPE culture medium composed of IMDM medium (Invitrogen) and keratinocytes medium (CellSystems ...
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bioRxiv - Microbiology 2019Quote: Total RNA was extracted from HIEs wells (in hW-CMGF+ or differentiation media for 4 days) or MA104 cells grown to confluency in a 6-well plate using TRIzol reagent (Ambion). Total RNA was treated with Turbo DNase I (Ambion ...
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bioRxiv - Systems Biology 2019Quote: ... for 30 minutes at 37C and plated with 2 million C57Bl/6 mouse embryonic fibroblasts as feeder cells (Gibco A34960) and with serum+LIF media composing of ...
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bioRxiv - Microbiology 2019Quote: ... Coverslips were mounted onto glass slides with ProLong Gold Anti-Fade reagent with DAPI (4[prime],6-diamidino-2-phenylindole; ThermoFisher). Images were obtained using an Olympus BX60 inverted microscope equipped with phase and fluorescence optics ...
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bioRxiv - Molecular Biology 2019Quote: ... 20ng of totalRNA was used for library preparation with ERCC Spike-in control Mix A (Ambion 10-6 final dilution). All steps were performed on a Tecan EVO150 ...
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bioRxiv - Genomics 2019Quote: ... A real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (# 4485694, Applied Biosystems, CA, USA) with the following reagents ...
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bioRxiv - Microbiology 2019Quote: ... pelleted again by centrifugation and resuspended at 3−5 × 106 cells/ml in sterile PBS containing 1.25 µg/ml 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI; ThermoFisher Scientific) and stored on ice until required ...
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Transient upregulation of translational efficiency in prodromal Tg2576 mice precipitates AD symptomsbioRxiv - Neuroscience 2019Quote: ... 5 μl of each fraction (polysomes and non polysomes) was used for First-strand synthesis using p(dN)6 and 100 U of M-MLV RT (Invitrogen). The same amount of first strand DNA was used for qRT-PCR analysis ...
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bioRxiv - Genetics 2020Quote: ... we reverse transfected approximately 3.0 x 105 - 5.0 x 105 293T/TLR cells with 75 nM of siRNA per well of a 6-well plate using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Abi3-WT and -KO MØPs were maintained in 6-well plates with Roswell Park Memorial Institute 1640 (RPMI; Gibco®, ThermoFisher) supplemented with 10% heat-inactivated Foetal Bovine Serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293 cells were grown on coverslips in 6 well dishes and transfected with pcDNA3.1-3XFLAG-ZNF507 plasmid using lipofectamine reagent (Thermo Fisher). After 36 hours cells were fixed with 4% paraformaldehyde in PBS for 10 minutes at room temperature ...
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bioRxiv - Genomics 2021Quote: ... these droplets were transferred at a density of 6 droplets per 1 well of a 6-well plate into STEP3 medium containing a 1:1 mixture of DMEM/F12 (Invitrogen) and Neurobasal with 0.5X N2 supplement (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... Ten-fold serial dilutions (1×10−1 to 1×10−6) of virus stocks were prepared in MEM (supplemented with 25 mM HEPES (Gibco), 2mM L-Glutamine (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... with 1.6 µM of specific primers with an QuantStudio™ 6 Flex system (Applied, Biosystems, Life Technologies, Saint-Aubin, France) in a final volume of 15µL ...
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bioRxiv - Cell Biology 2021Quote: ... For experiments glass-bottomed imaging dishes were exposed to deep UV light for 6 minutes and coated with Poly-D-Lysine (ThermoFisher) for a minimum of 20 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... was crosslinked to the PAAm gels using N-sulfosuccinimidyl-6-(4′-azido-2′-nitrophenylamino)hexanoate (sulfo-SANPAH, from Life Technologies). 30 μL of 1mg/mL sulfo-SANPAH dissolved in milliQ H2O was added to PAAm gels which were irradiated under a 365nm UV lamp (ABM ...
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bioRxiv - Immunology 2021Quote: ... and primers and a FAM-ZEN/Iowa Black FQ labeled probe sequence (IDT) on the QuantStudio 6 system (Applied Biosystems). An Ultramer DNA oligo (IDT ...