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Citations for Thermo Fisher :
3051 - 3100 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: All primers and probes were diluted to a concentration of 20 μM using nuclease-free water (Ambion, ThermoFisher Scientific, California, USA). Four master mixes for the two multiplex real-time PCR assays (Regional assay and Clade assay ...
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bioRxiv - Neuroscience 2022Quote: ... Mass spectrometry experiments were performed using liquid chromatography (LC/MS) using an Acquity M-Class UPLC (Waters) followed by mass spectrometry using an Orbitrap Q Exactive HF-X (Thermo Scientific). For a typical LC/MS experiment ...
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bioRxiv - Neuroscience 2022Quote: ... After the elution with DNase/RNase free water for 2 min at RT the sample volumes were reduced using a SpeedVac (Thermo Scientific). The RNA concentration was measured using NanoDrop2000 (Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... The 140-min water-acetonitrile gradient was delivered using a Dionex Ultimate 3,000 ultra high-performance liquid chromatography system (Thermo Fisher Scientific) at a flow rate of 200 nl/min (Buffer A ...
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bioRxiv - Synthetic Biology 2022Quote: ... Round one PCR products were purified using AMPure XP beads following the manufacturer’s protocol and eluted in 25 μL UltraPure Water (Thermo Fisher Scientific). 2 μL was used as input in a second round of PCR to attach on Illumina adaptors and dual index primers (NEB ...
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bioRxiv - Synthetic Biology 2022Quote: ... The round two PCR products were purified using AMPure XP beads following the manufacturer’s protocol and eluted in 25 μL UltraPure DNase/RNase-Free distilled water (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Gels were run until the 37 kDa marker reached the bottom third of the gel and were immediately washed with water prior to incubation at room temperature in 1X renaturing buffer (Novex, ThermoFisher, LC2670) for 30 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... we used bath applications of waterborne copper sulfate (CuSO4, 50 µM in system water or 10 µM in E3 medium, for 2 hours at 28.5°C, Acros Organics, 10413465) to achieve cell-specific ablation or BAPTA (5 mM ...
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bioRxiv - Neuroscience 2022Quote: ... to achieve cell-specific ablation or BAPTA (5 mM, 20 min in either system water or E3 medium at 28.5°C, Thermo Fisher, B1204) to disrupt mechanoelectrical transduction specifically ...
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bioRxiv - Neuroscience 2022Quote: ... RNA was eluted in 50 μl water and precipitated with 0.75 M ammonium-acetate and 10 μg glycogen (ThermoFisher, Cat# R0551) by adding 125 μl ethanol (100 %) ...
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bioRxiv - Cancer Biology 2022Quote: ... the polar metabolite phase was resuspended in 50 μL water and analyzed in a Dionex UltiMate 3000 LC System (Thermo Scientific) with a thermal autosampler set at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... and dialyzed against ultra-pure water (UPW) for 36 h using SnakeSkin Dialysis Tubing (molecular weight cut-off: 3.5 K, 11532541, Thermo Fisher Scientific). The dialyzed SF solution was frozen at -80° C and lyophilized for 7 days ...
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Simultaneous adjunctive treatment of malaria and its co-evolved genetic disorder sickle cell anaemiabioRxiv - Microbiology 2022Quote: ... Serial dilutions of Pf3D7 total RNA and DNA (ranging from 8 to 5000 pg/µl) with nuclease-free water (AM9935, Life Technologies) were used to generate a relative standard curve for pfr1 ...
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bioRxiv - Biophysics 2022Quote: ... Frogs are euthanized by immersion for 10 min in 1 L water + 5 g Tricaine-S (Pentair via Fisher Scientific #NC0342409) + 10 g sodium bicarbonate (Fisher Scientific # MK-7396-500) ...
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bioRxiv - Immunology 2024Quote: ... 1.8 µm) the outlet of which was connected to an LTQ-Orbitrap XL MS System (ThermoFisher Scientific, USA and Waters, USA). The LC flow rate was set to 150µL/min and the column oven temperature was adjusted to 35°C ...
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bioRxiv - Neuroscience 2024Quote: ... the RNA pellets were dissolved in RNase/DNase free ultrapure water and quantified using a NanoDrop 2000 spectrophotometer (Thermo Fisher Scientific). One microgram of RNA was converted into cDNA using a High-Capacity cDNA Reverse Transcription kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/).
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bioRxiv - Neuroscience 2024Quote: NanoLC–MS/MS analyses were conducted using a nanoAcquity UPLC system (Waters) coupled with a Q-Exactive HF-X Mass Spectrometer (Thermo Scientific) equipped with a Nanospray Flex ion source ...
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bioRxiv - Cell Biology 2023Quote: ... MS experiments were performed using liquid chromatography (LC) with an Acquity M-Class UPLC (Waters) followed by MS using an Orbitrap Fusion Tribrid MS (Thermo Scientific). A flow rate of 300 nL/min was used ...
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bioRxiv - Microbiology 2024Quote: ... IFNβ, IFNα and IFIT1) and 15x (for reference genes: ACTIN, RPL13A, YWHAZ and UBC) with Nuclease-free water (Ambion, Life Technologies). 5 μL of the diluted cDNA was then used for qPCR ...
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bioRxiv - Molecular Biology 2024Quote: ... Digestions were incubated for 1 hour at 37 ℃ and then diluted 1:5 with UltraPure water before loading on E-gel EX 2% Agarose gels (Invitrogen, G401002). Backbones were purified using the Monarch Gel Extraction Kit (Monarch) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We eluted the RNA in 40 μl of DNase/RNase-free water and treated it with DNase I (Thermo Scientific, USA) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2023Quote: ... the samples were washed three times with PBS and additional once with water before the samples were mounted in ProLong TM Gold antifade reagent (Thermo Fisher). The coverslips were dried for at least 24 h ...
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bioRxiv - Microbiology 2024Quote: ... The isolated total RNA was eluted in ribonuclease-free water and RNA concentrations were subsequently quantified using a NanoDrop One (Thermo Fisher). cDNA was synthesized from extracted RNA (typically 100 ng ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Microbiology 2024Quote: ... the mixture was extensively dialyzed in milli-Q water using a membrane with molecular weight cutoff of 3.5 kDa (Spectra/Por, Thermo Fisher Scientific) at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... embryos were manually dechorionated and placed in either 5 mL of egg water (control group) or 5 mL of 40 µM MK-801(Fisher Scientific) in egg water ...
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bioRxiv - Immunology 2024Quote: ... PBLs were thawed for 2min with gentle agitation in a 37°C water bath and then washed twice with complete DMEM (Thermo Fisher) supplemented with nonessential amino acids (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Bleaching of the fluorochromes were achieved by submerging the slide in a 1mg/mL solution of lithium borohydride in water (Acros Organics) for 15 minutes at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were dissolved in 10 µL of reconstitution buffer (96:4 water: acetonitrile, 1% formic acid and analyzed by LC-MS/MS using QExactive (Thermo Fisher) in the proteomics core facility at EMBL Heidelberg (https://www.embl.org/groups/proteomics/) ...
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bioRxiv - Immunology 2024Quote: ... The cDNA generated was then diluted 2-5 fold in RNase- and DNase-free water and mixed with PowerUP SYBR Green Master Mix (Applied Biosystems) and primers (listed in Supplemental Table 3 ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Biochemistry 2024Quote: ... Biolog Microbial Phenotyping Microarray Plates PM1-PM6 were resuspended in 125 μL MilliQ water and 5 μL of each well was aliquoted into MicroAmp Optical 96-Well Reaction Plates (ThermoFisher Scientific) to prepare screening plates ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of 1:100 diluted fluorescent beads in DI water (FluoSpheres carboxylate-modified 0.2 µm, 660/680, F8807, Thermo Fisher Scientific), 6 µL 10% v/v tetramethylethylenediamine (TEMED ...
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bioRxiv - Bioengineering 2024Quote: ... water and then dehydrated through graded ethanol series (in %: 50, 70, 80, 90, 95 and 100) (Thermo-Fisher Scientific, Waltham, MA) before proceeding with Critical Point Dryer (CPD ...
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bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
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bioRxiv - Molecular Biology 2023Quote: ... The samples were analyzed using an HPLC-ESI-MS/MS system consisting of a high-performance liquid chromatography (nanoAcquity, Waters) set in line with an electrospray ionization (ESI) Orbitrap mass spectrometer (QE-HF orbitrap, ThermoFisher Scientific). A 100μm id × 365μm od fused silica capillary micro-column packed with 20cm of 1.7μm diameter ...
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bioRxiv - Molecular Biology 2023Quote: ... and an aliquot ranging from 5 to 25% was analyzed on a nanoAcquity UPLC (Waters Inc.) connected to a Q Exactive mass spectrometer (Thermo Scientific) equipped with a Digital PicoView source (NewObjective) ...
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bioRxiv - Cell Biology 2023Quote: ... The volume was brought to 250μL with nuclease-free water and RNA was extracted with 25:24:1 Phenol-Chloroform-Isoamyl alcohol (Invitrogen, #15593-031) using a MaXtract tube ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA pellets were resuspended in 25 mL DEPC water at 37°C with shaking at 1000 rpm for 15 min and concentrations were determined using a NanoDrop (ThermoFisher Scientific). We added 22 mL denaturing mix (15 mL formamide ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was eluted with 20 μl of RNase-free water and yield was determined fluometrically using Qubit RNA high-sensitivity assay kit (Thermo Fisher).
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bioRxiv - Microbiology 2023Quote: ... Cells were then washed twice with distilled water and incubated with a 1:3,000 dilution of protein A-HRP (Thermo Fisher Scientific). After 1 hour ...
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bioRxiv - Systems Biology 2022Quote: NanoLC-MS/MS analyses were performed on a nanoACQUITY Ultra-Performance LC system (Waters, Milford, MA) coupled to a Q-Exactive Plus Orbitrap mass spectrometer (ThermoFisher Scientific) equipped with a nanoelectrospray ion source ...
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bioRxiv - Pathology 2022Quote: ... The RNA was dissolved in 30-50 μl RNase-free water and the consistency was analyzed by NanoDrop 2000 equipment (Thermo Fisher).
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bioRxiv - Evolutionary Biology 2023Quote: ... each frog was transferred to an individual 250 mL container containing sterile distilled water (the Millipore Milli-Q™; Fisher Scientific) to remove transient microbes from the environment ...
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bioRxiv - Genomics 2023Quote: ... Protein digests were analyzed by LC/MS on either a Premier Q-Tof mass spectrometer (Waters Corp.) or a Q Exactive Plus mass spectrometer (ThermoFisher Scientific) and a UPLC system equipped with a 75µm x 150µm BEH dC18 column (1.8µm particle ...
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bioRxiv - Biochemistry 2023Quote: ... protein mixtures was conducted on a NanoAcquity Ultra Performance chromatographic system (Waters Corp., Milford, MA, US) coupled to an Orbitrap Fusion Lumos tribrid mass spectrometer (Thermo Scientific). We used the Advion TriVersa NanoMate (Advion ...
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bioRxiv - Neuroscience 2023Quote: ... cells were rinsed with Milli-Q water and mounted on a glass slide containing a drop of ProLong Diamond Antifade Mounting media (Thermo Fisher). Mounting Media was allowed to solidify for 24 hours at room temperature in the dark before proceeding to STED imaging.
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bioRxiv - Neuroscience 2023Quote: ... HD-MEA chips were then washed with sterile water three times before adding 1 ml of complete cell culture media consisting of Neurobasal supplemented with B27 (Gibco, ThermoFisher Scientific) and 10% horse serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... PEG-6000 (15% w/v) was dissolved in water and filtered through 0.2-micron Sartorius Minisart™ Plus Syringe Filters (Fisher Scientific). 50 mL of the filtered solution was overlaid onto the surface of solidified Murashige & Skoog medium ...