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Citations for Thermo Fisher :
3251 - 3300 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... RNA was stored at -80°C in nuclease-free water and concentration was determined with a Nanodrop 1000 instrument (Thermo Fisher Scientific). Samples were sent to a sequencing facility (Novogene Corporation Inc. ...
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bioRxiv - Biochemistry 2022Quote: ... washed in TEV buffer solution (water, TEV buffer, 100 μM dithiothreitol) and resuspended in buffer with Ac-TEV protease (Invitrogen, 12575-015) and incubated overnight ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were supplemented with 150 fmol of internal standard (yeast ADH1, Waters) and subjected to tandem mass tag (TMT)-labeling using TMT 6-plex reagents (Thermo Fisher Scientific). TMT 126-128 were used for the reference (Vehicle or WT ...
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bioRxiv - Molecular Biology 2022Quote: ... several concentrations of each siRAB18 molecule (0.24nM to 50nM) or sterile water (negative control) was individually reverse transfected in duplicate into Hep3B cells using lipofectamine RNAiMAX (Invitrogen, cat#13778075). 24 hours post-transfection ...
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bioRxiv - Immunology 2022Quote: Cryopreserved samples were rapidly thawed in a 37°C water bath and resuspended in complete RPMI supplemented with 10% FBS and 50 units/mL of penicillin–streptomycin (Thermo Scientific HyClone). Cell suspensions were processed and stained as previously described (44 ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves discs were allowed to recover overnight in water and assayed in 20 µg/mL HRP and 6 µM L-012 in a 96-well luminometer plate (Thermo Fisher Scientific), with or without 100 nm flg22 ...
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bioRxiv - Immunology 2022Quote: ... Peptides were desalted using Sep-Pak® Light C18 Cartridge (Waters, Wilmslow, UK, WAT023501) and dried down using a SpeedVac (Thermo Scientific).
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bioRxiv - Microbiology 2021Quote: ... Samples were injected in randomized order at a volume of 10 μL and eluted using a polar to apolar gradient formed using Ultrapure water (A) and LC/MS grade acetonitrile (Thermo Fisher Scientific) (B) ...
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bioRxiv - Microbiology 2021Quote: ... spun down the sample at 17,000 x g and replaced residual sorting buffer by DEPC treated water (Invitrogen Cat. No. 46-2224). The samples were stored in approx ...
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bioRxiv - Microbiology 2021Quote: ... The annealed product was diluted 1 in 200 in sterile distilled water and used for directional cloning by ligating (Thermo Scientific FEREL0011) to 60 ng of BsaI-digested ...
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Evolutionarily divergent mTOR remodels the translatome to drive rapid wound closure and regenerationbioRxiv - Developmental Biology 2021Quote: ... air dried and resuspended in 10 μL (for fractions) or 40 μL (for input) of Ultra Pure DNase/RNase-Free Distilled Water (Invitrogen, 10977-015). For each time-point ...
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bioRxiv - Molecular Biology 2020Quote: ... All DNA/RNA oligonucleotides and plasmids were diluted with RNase-free water and the concentrations were determined by Nanodrop 200 (Thermo Fisher, USA). All of these were stored at −20°C until use ...
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bioRxiv - Molecular Biology 2020Quote: ... to 40% of solvent B (acetonitrile with 0.1% formic acid) for 100 min were applied on nanoACQUITY UPLC (Waters) coupled with Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific), which was operated at sensitive mode using the following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were injected onto the 2D-nanoAquity UPLC (Waters, Corp., Milford, USA) coupled online with a Q Exactive Plus system (Thermo Scientific, USA) in nanoelectrospray positive ion mode ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae and juveniles were paralyzed by immersion in α-bungarotoxin (20 µl of 1 mg/ml solution in system water, B1601, ThermoFisher Scientific) (Duboué et al. ...
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bioRxiv - Plant Biology 2021Quote: ... Each sample was placed in a one cm plastic spectrophotometer cuvette and absorption spectra recorded from 480-800 nm versus a distilled water blank using a Genesis 10 UV Instrument (Thermo Fisher Scientific). The spectra were analyzed utilizing the Vision Lite software (Thermo Fisher).
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bioRxiv - Molecular Biology 2020Quote: ... The RNAs and DNAs were dissolved in nuclease-free water and their concentration and purity established by spectrophotometry (Nanodrop 1000, Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... Stained sections were washed with PBS and a final rinse with water to minimize salt precipitation and mounted using ProLong® Glass antifade reagent (Invitrogen, ThermoFisher) and Fisher Finest thick coverslips (ThermoFisher) ...
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bioRxiv - Microbiology 2020Quote: ... The UPLC system consisted of 0.1% formic acid (mobile phase A) in ultrapure water and a mobile phase B consisting of 0.1% formic acid (Fisher Scientific, Loughborough, UK) in acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... N2-rich water (3.50 mg/L) and normal water without treatment (Air) (8.75 mg/L) was determined using an oxygen electrode (Thermo Fisher Scientific, USA).
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bioRxiv - Neuroscience 2020Quote: ... RNA samples were eluted in 30-50 µL of RNase free water and analyzed on a NanoDrop™ One Microvolume UV-Vis Spectrophotometer (Thermo Scientific). RNA was then either used immediately for cDNA synthesis or stored at -80°C ...
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bioRxiv - Developmental Biology 2022Quote: ... eggs were individually homogenised in 5ul DEPC water within the PCR tube and 1ul was used for gDNA extraction using Phire Animal Tissue Direct PCR Kit (ThermoFisher, MA, USA) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... hyperglycemia was induced either by allowing mice to consume D30 (dextrose 30% water) or by the administration of streptozotocin (Thermo Scientific, S0130) starting two weeks before cancer cell implantation ...
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bioRxiv - Cell Biology 2022Quote: ... then coverslips were dipped in PBS and water 10 times each and mounted on glass microscope slides using Prolong® Gold antifade reagent (Life Technologies). Cells were imaged with a 63× oil 1.4 numerical aperture (NA ...
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bioRxiv - Cell Biology 2022Quote: ... the peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were resuspended in 500 μl of 4% paraformaldehyde (dissolved in sea water) and fixed for 20 min on Superfrost plus slides (Thermo Scientific, J1800AMNZ) at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... This was added dropwise (100 µL / min) to purified water (4 mL) with Pluronic F-68 (100 µL; Life Technologies, Carlsbad, CA). Each batch of particles was stirred for 2 hours prior to centrifugation at 7356 RCF for 15 minutes prior to washing with purified water and centrifuging again ...
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bioRxiv - Synthetic Biology 2020Quote: ... Samples were serially diluted 1000-fold with sterile DI water before analyzing them in a Thermo Attune NXT flow cytometer (ThermoFisher Scientific, MA). Samples were run at a 12.5 μl/min flow rate ...
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μg peptides were resuspended in 1% formic acid diluted in water and loaded on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). Settings used were as previously described [22] ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1-10 ng template in 9 μl Nuclease-free water were added to 10.0 μL of 2× TaqMan® Universal PCR master mix (ABI/Life technologies, USA) and 1.0 μL of a 20× combined primers and probes mix (ABI/Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: Total RNA was extracted from young leaves between 24 and 120 h after treatment with water or 600 μg.ml−1 pGM using TRIzol® Reagent (AmbionRNA by Life Technologies™) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... GBM cells in ultrapure water were then sonicated for 10 minutes and used for dsDNA quantification using the Quant-iT PicoGreen dsDNA kit (Molecular Probes, Invitrogen), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified RNA was eluted in 40 μl of nuclease-free water and then was quantified using a Nanodrop spectrophotometer (Thermo Fisher Scientific). For analysis of tethered function MS2 reporter mRNAs by RT-qPCR ...
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Suppressing STAT3 activity protects the endothelial barrier from VEGF-mediated vascular permeabilitybioRxiv - Molecular Biology 2020Quote: ... and a mixture of 2000 kDa FITC-dextran and 70 kDa Texas Red-dextran (2 mg/ml in embryo water; Life Technologies, Inc.) was injected ...
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bioRxiv - Neuroscience 2020Quote: ... and loaded onto an Acquity UPLC® M-Class CSH™ C18 column (Waters) for offline reversed-phase high pH fractionation using a Dionex Ultimate 3000 HPLC system (Thermo Scientific). Peptides were separated by increasing concentrations of buffer B (80 % ACN ...
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bioRxiv - Microbiology 2021Quote: ... were diluted 1:1000 in milliQ water and left to adhere on a poly-L-lysine glass slide (Thermo Scientific, Menzel-Gläser) for 1 h ...
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bioRxiv - Pathology 2021Quote: ... The resulting pellet was diluted in 50 µl of molecular grade water and DNA concentration quantified using NanoDrop™ (Thermo Fisher Scientific). T ...
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bioRxiv - Molecular Biology 2021Quote: ... was dissolved in 9 ml of deionized water and 1ml of 10x Pierce™ Stable Peroxide Substrate Buffer (Thermo Fisher, Scientific, 34062). 100 μl of OPD substrate solution were added per well and incubated for 15 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... The negative controls of our RT-PCR reactions were formed from the same reaction mix as our samples switching only the 5 uL of DNA by 5 uL of ultrapure™ DNase/RNase-Free Distilled Water (Invitrogen France), thus one negative control is placed after every 5 samples on a Light cycler 480 multiwell plates 96-well plate (Roche).
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bioRxiv - Systems Biology 2020Quote: ... Tryptic peptides were desalted with Oasis HLB 1 cc/10 mg cartridges (Waters 186000383) and dried with a tabletop speed vacuum (Thermo Scientific SPD1010), then re-suspended in 40 μl of 5% mass spectrometry grade acetonitrile (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... samples were washed 2 times with 70% ethanol and the final RNA pellets were diluted in ultra-pure RNase free water (Invitrogen, 10977-035). RNA concentration was calculated using Nanodrop (ThermoFisher Scientifics).
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bioRxiv - Cell Biology 2021Quote: ... Each sample was separated on a Nanoacquity™ (Waters) Ultra Performance Liquid Chromatography column coupled to an LTQ-Orbitrap XL (Thermo Fisher) equipped with a nanoelectrospray source (Proxeon) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Dried fractions were reconstituted with 2% ACN-0.1% FA and analyzed by LC-MS/MS using a nanoACQUITY system (Waters) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Peptides were separated using an analytical C18 Acclaim PepMap column (75μm × 250 mm ...
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bioRxiv - Physiology 2020Quote: ... UK) (1 µg/ml PK, 50 mM Tris-HCl [pH 7.5]; 6.25 mM EDTA in autoclaved water; Thermo Fisher Scientific, Oxford, UK) at 37°C for 12 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... using a nanoACQUITY (Waters Corporation, Milford, MA, USA) online coupled with an Orbitrap Velos Pro hybrid ion trap mass spectrometer (Thermo Fisher Scientific). For each LC-MS/MS analysis ...
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bioRxiv - Biochemistry 2021Quote: ... These peptide mixtures were desalted using Oasis HLB cartridges (Waters, Milford, MA) and dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany). The digested peptides (n=30 ...
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bioRxiv - Microbiology 2021Quote: High resolution MS identification and fragmentation was carried out using either a quadrupole-time-of-flight MS (Q-TOF, Waters Synapt G2-Si) or an Orbitrap Fusion (Thermo Fisher Scientific) by direct infusion and collision induced dissociation (CID) ...
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bioRxiv - Microbiology 2021Quote: ... and analyzed by nano-LC/MS/MS using a Waters NanoAcquity HPLC system interfaced to a Q Exactive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Scientific) [111] ...
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... on a nanoAcquity system (Waters) coupled to a Q-Exactive mass spectrometer equipped with a nanoelectrospray ion source for automated MS/MS (Thermo Fisher Scientific). High-resolution MS/MS spectra were processed with MaxQuant (version 1.6.7.0 ...
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bioRxiv - Immunology 2021Quote: ... Five µL of the purified peptides were injected into a high-performance liquid chromatography system (ACQUITY UPLC: Waters) connected to a triple-quadrupole mass spectrometer (TSQ Vantage; Thermo Fisher Scientific) with an ion source of electrospray ionization in multiple reaction monitoring (MRM ...