Labshake search
Citations for Thermo Fisher :
3201 - 3250 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... Biopsy punch samples 200 – 400 μm thick were dehydrated in an ascending series of ethanol dilutions in water (50%, 70%, 90%, and 2 x 100%) containing 1 mM dithiothreitol (DTT; ThermoFisher Scientific) for 40 seconds per step with the temperature restriction at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... The supernatants were harvested by centrifugating 10min at 3000 x g and dialyzed against water overnight at 4°C in Snakeskin dialysis tubing cut off 10kDa (68035, ThermoFisher Scientific). Precipitated S-layer proteins were recovered by centrifugation at 20,000 x g for 20min ...
-
bioRxiv - Bioengineering 2024Quote: ... Plate was washed 3 times with 300 µL/well with ultrapure water (cat 10977-015-023, Thermo Fisher Scientific, MA, USA). Blocking was performed with 100 µL of chonblock (cat 50-152-6971 ...
-
bioRxiv - Bioengineering 2024Quote: ... Cells were spun down and lysed for 4 min using chilled lysis buffer that consisted of nuclease-free water (Cat: J71786.AE, Thermo Fisher), Tris-HCL (10 mM ...
-
bioRxiv - Biophysics 2024Quote: ... 1 µL of the completed PCR reactions was then combined with 4 µL of water and 5 µL of print buffer (2x: 20 mg/mL UltraPure BSA (Thermo Fisher), 24 mg/mL trehalose dihydrate ...
-
bioRxiv - Cancer Biology 2024Quote: LC-MS analysis was used to measure water-soluble metabolits by running samples on the Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled with hydrophilic interaction chromatography (HILIC ...
-
bioRxiv - Cancer Biology 2024Quote: ... samples were thawed to 37°C in a water bath and rinsed in “FACS buffer” (1x Ca2+ and Mg2+ deficient HBSS (ThermoFisher Scientific) supplemented with 1% BSA (Research Products International)) ...
-
bioRxiv - Bioengineering 2024Quote: ... total RNA was diluted to a concentration of 1 µg RNA/12.2 µL total sample volume in DEPC-water (Invitrogen, Cat# 750024). All RNA samples first underwent a DNAse step (1 µL DNAse/12.2 µLRNA ...
-
bioRxiv - Cancer Biology 2024Quote: ... Concentrations of purified RNA in nuclease-free water were determined by Nanodrop-2000 Spectrophotometer and Qubit RNA BR Assay (ThermoFisher Scientific). RINe numbers ranging from 7.8-10 were determined by TapeStation 4150 RNA ScreenTape Analysis (Agilent Technologies ...
-
bioRxiv - Cell Biology 2024Quote: ... iPSCs were thawed in a 37°C water bath and immediately transferred to room temperature Essential 8 (E8) medium (Gibco, USA) to dilute the freezing media ...
-
bioRxiv - Biochemistry 2024Quote: TMT-labeled peptides were fractionated by high-pH reversed phase separation using a XBridge Peptide BEH C18 column (3.5 µm, 130 Å, 1 mm x 150 mm, Waters) on an Ultimate 3000 system (Thermo Scientific). Peptides were loaded on column in solution A and the system was run at a flow of 42 µl/min ...
-
bioRxiv - Bioengineering 2024Quote: ... The isolated PM was then redispersed in DI water and sonicated for 5 min using a bath sonicator (Fisher Scientific FS30D) at 42 kHz and 100 W ...
-
bioRxiv - Biochemistry 2024Quote: Fractions were analyzed by HPLC-MS/MS using a system consisting of an HPLC (nanoAcquity, Waters) connected to an electrospray ionization (ESI) Orbitrap mass spectrometer (QE-HF, ThermoFisher Scientific). HPLC separation employed a 365 μm diameter fused silica capillary micro-column packed with 20 cm of 1.7μm-diameter ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA was diluted 1:5 in nuclease-free water and 2 µL was mixed with 10 µL 2X SYBR Select MasterMix (ThermoFisher 4472908) and 250 nM forward and reverse qPCR primers in a 20 µL final volume per sample ...
-
bioRxiv - Cancer Biology 2024Quote: ... 0.2 mM dNTPs, 0.1 µM primer forward and reverse mix, 2 U DreamTaq DNA Polymerase, in nuclease-free water; Thermo Scientific, EP0703). PCR primer and thermal cycling conditions are detailed in Supplementary Tables 1 and 2 ...
-
bioRxiv - Cell Biology 2024Quote: ... immunocomplexes were eluted by boiling the beads in elution buffer (20% LDS with 10% DTT in Milli Q water) and resolved on NuPAGE Novex 4– 12% Bis–Tris Protein Gels (Life Technologies). Proteins were transferred onto PVDF membranes using the XCell II Blot module (Life Technologies ...
-
bioRxiv - Cell Biology 2020Quote: ... A 140-minute water-acetonitrile gradient was delivered to a maximum of 80% buffer B using a Dionex Ultimate 3000 UHPLC system (Thermo Fisher Sci.) at a flow rate of 300 nl/minute (Buffer A ...
-
bioRxiv - Cell Biology 2020Quote: ... ATP was extracted by a boiling water method56 and measured using a luciferin–luciferase ATP determination kit (Molecular Probes, Eugene, OR, USA).
-
bioRxiv - Microbiology 2021Quote: ... 12 ng/mL DNA template and 2.5 units Bioline Taq DNA Polymerase (Taunton, MA, USA) in UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen, Carlsbad, USA).The PCR amplicons were purified using the MO BIO UltraClean™ PCR CleanUp Kit (Carlsbad ...
-
bioRxiv - Developmental Biology 2020Quote: ... 70 μL of lysate was diluted in 55 μL of water and stored at -80°C in 375 μL of TRIzol LS (Thermo Fisher, 10296010). For ribosome profiling ...
-
bioRxiv - Neuroscience 2021Quote: ... sections were fast washed in PBS and milliQ water and mounted on 76 × 26 mm gelatine-coated microscope slides (Thermo Fisher Scientific), air-dried ...
-
bioRxiv - Cancer Biology 2020Quote: ... the cell extract was dissolved in 15 μL water and 15 μL methanol/acetonitrile (1:1, v/v) (LC-MS optima grade, Thermo Fisher Scientific). Samples were centrifuged at 20,000g for 2 minutes at 4°C ...
-
bioRxiv - Molecular Biology 2021Quote: ... washed rapidly in water and frozen in liquid N2 or fixed in RNAlater following the manufacturer’s instructions (ThermoFisher Scientific, Waltham, Massachusetts, US); in short ...
-
bioRxiv - Cell Biology 2022Quote: ... frozen WTC11 cells were first thawed in 37 °C water bath and washed in Essential 8 Medium (E8; Thermo Fisher Scientific, #A1517001) by centrifugation ...
-
bioRxiv - Immunology 2022Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and QuantiStudio 5 Real-Time PCR machine (Applied Biosystems) ...
-
bioRxiv - Biochemistry 2020Quote: Dried samples were reconstituted in 100mM ammonium formate pH ∼10 and analyzed by 2DLC-MS/MS using a 2D nanoACQUITY Ultra Performance Liquid Chromatography (UPLC) system (Waters corp., Milford, MA) coupled to an Orbitrap Velos Pro mass spectrometer (Thermo Fisher Scientific). Peptides were loaded onto the first dimension column ...
-
bioRxiv - Systems Biology 2020Quote: Water (LC-MS Grade, Optima; 10509404), Acetonitrile (LC-MS Grade, Optima; 10001334) and Formic acid (LC-MS Grade, Thermo Scientific Pierce; 13454279) were purchased from Fisher Chemicals ...
-
bioRxiv - Immunology 2020Quote: ... the pellet was dissolved in 25μl ultrapure water and the mRNA concentration measured using a NanoDrop (Thermo Fisher Scientific, Rochester, NY, USA). mRNA purity was assessed using the 260/280 nm and 260/230 nm ratios and the samples were used with a purity range of 1.8 to 2.
-
bioRxiv - Cancer Biology 2020Quote: ... and processed for LC-MS/MS analysis using a nanoACQUITY system (Waters) coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific) as previously described45 ...
-
bioRxiv - Genetics 2020Quote: ... The particle-free supernatant was analyzed by LC-MS/MS in the Laboratory of Mass Spectrometry (IBB PAS, Warsaw) using a nanoAcquity UPLC system (Waters) coupled to an Orbitrap QExative mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in the data-dependent MS2 mode ...
-
bioRxiv - Genomics 2021Quote: ... Elution was performed in nuclease-free water and 1 μL was used for measuring the concentration with the Qubit™ dsDNA High Sensitivity Assay Kit (ThermoFisher Scientific), while the size was assessed with a High Sensitivity DNA chip on an 2100 Bioanalyzer.
-
bioRxiv - Developmental Biology 2020Quote: ... Slides were cooled for 5 minutes under cold running tap water and washed for 5 minutes each in de-ionized water and PBS on a shaker before circumscribing with a Super PAP pen (Thermo Fisher Scientific) and blocking ...
-
bioRxiv - Immunology 2021Quote: Cryopreserved samples were rapidly thawed in a 37°C water bath and resuspended in complete RPMI supplemented with 10% FBS and 50 units/mL of penicillin–streptomycin (Thermo Scientific HyClone). Cell suspensions were processed and stained as previously described [14 ...
-
bioRxiv - Microbiology 2021Quote: Water samples for enterococcus and Escherichia coli were aseptically collected and brought back to the laboratory using Thermo Scientific Security-Snap Coliform Polypropylene Water Sample Bottles with 10mg sodium thiosulfate (Thermo Fisher Scientific) and kept in ice (below 8°C) ...
-
bioRxiv - Neuroscience 2021Quote: ... total nucleic acids were resuspended in 50 μL ultrapure water and treated with DNAse I (Turbo DNA Free® kit; AM1907, Ambion®) to eliminate any trace of possible genomic DNA contamination ...
-
bioRxiv - Cell Biology 2021Quote: ... under humidified standard condition at 37 °C in 5% CO2 atmosphere (Forma water jacketed CO2 incubator, 3111TF; Thermo Fisher Scientific, Waltham, Massachusetts). When the cells reached 80% confluence ...
-
bioRxiv - Cell Biology 2021Quote: ... Human onTARGETplus siRNA were diluted to 20 µM stock in RNase-free water and cells were transfected with 10 nM siRNA for 3 days with Lipofectamine RNAiMAX transfection reagent (Thermo Fisher, 13778075) in 6 well format ...
-
bioRxiv - Plant Biology 2020Quote: Samples were analyzed by a nanoflow liquid chromatography system (nanoAcquity; Waters) combined with an electrospray ionization FT/ion-trap mass spectrophotometer (LTQ Orbitrap Velos; Thermo Fisher Scientific). The LC system employed a 100 × 365 -μm fused silica microcapillary column packed with 15 cm of 3-μm-diameter ...
-
bioRxiv - Microbiology 2020Quote: ... mice were anesthetized by intraperitoneal (i.p) injection of ketamine and xylazine diluted in water for injection (WFI; Thermo Fisher Scientific, Waltham, MA). Ad-Empty at 2.5×108 PFU ...
-
bioRxiv - Microbiology 2020Quote: ... cDNA was diluted 1:5 in water and 2 μl was used as a template for real-time PCR using SYBR® Green PCR master mix (Applied Biosystems) and a 7900HT Real-Time PCR machine (Applied Biosystems) ...
-
bioRxiv - Plant Biology 2020Quote: ... and then re-suspended in 30 μL of UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen™, Thermo Fisher Scientific, MA, USA). RNA was treated with DNase I (InvitrogenTM ...
-
bioRxiv - Plant Biology 2020Quote: ... and then re-suspended in 30 μL of UltraPure™ DNase/RNase-Free Distilled Water (Invitrogen™, Thermo Fisher Scientific, MA, USA). RNA was treated with DNase I (InvitrogenTM ...
-
bioRxiv - Plant Biology 2020Quote: ... 2 μL of DNA solution from the extraction and 10.8 μL of MQ purified water (obtained from a Barnstead GenPure Pro system, Thermo Scientific, Langenselbold, Germany) in the TBT-PAR water mix (Samarakoon et al. ...
-
bioRxiv - Plant Biology 2020Quote: ... 2 μL of DNA solution from the extraction above and 10.8 μL of either MQ purified water (obtained from a Barnstead GenPure Pro system, Thermo Scientific, Langenselbold, Germany), or the TBT-PAR water mix (Samarakoon & al. ...
-
bioRxiv - Microbiology 2020Quote: ... Stock solutions of the assay compounds were prepared by dissolving each into Milli-Q water and subsequently filter-sterilizing through 0.2-μm membrane Nalgene syringe filters (Thermo Scientific, NY, USA). Liquid cultures were grown from single colonies in marine broth until an OD600 ~0.3 was reached ...
-
bioRxiv - Immunology 2021Quote: ... the NBF was decanted and the cells were washed twice with 1X phosphate-buffered saline (PBS) diluted with purified water from a 10X stock solution (Fisher Scientific #BP3994). The cells were then permeabilized with 0.25% Triton buffer in 1X PBS (Fisher Scientific #PR-H5142 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Primary antibody was removed and plates were washed twice with 150 μL/well of 1x DPBS+Tween20 (TEKnova Cat # P0297, diluted to 1x in distilled water GIBCO #15230-147), before adding 50 μL/well of a 1:5000 dilution of HRP-conjugated goat anti-mouse IgG (Southern Biotech ...
-
bioRxiv - Cell Biology 2020Quote: ... We used a C Apochromat 63 X water immersion objective and cells cultured in Lab-Tek II chambered coverglasses (Nunc, Roskilde, Denmark) were imaged by CLSM methods ...
-
bioRxiv - Biochemistry 2022Quote: Dry peptides were reconstituted in 5 μl water and labeled by adding 2 μl TMT label (20 μg/μl in acetonitrile (ACN)) (TMTpro 16 plex, Thermo Fisher Scientific) and incubating 1 h at room temperature ...
-
bioRxiv - Biochemistry 2022Quote: The protein mass was determined on a Synapt G2-Si Q-ToF mass spectrometer (Waters) coupled to an Ultimate 3000 HPLC system (Dionex, Thermo Fisher Scientific) via a ZSpray ESI source (Waters) ...