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Citations for Thermo Fisher :
2851 - 2900 of 3690 citations for CdSeTe ZnS Quantum Dots NIR region Water solvent 760nm since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... with 40 μL of mobile phase A (0.1% FA in HPLC grade water) at 30 μL/min and separated using a 50 cm EASY-Spray column (ES803, ThermoFisher) ramping mobile phase B (0.1% FA in HPLC grade acetonitrile ...
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bioRxiv - Neuroscience 2024Quote: ... the samples were washed once with 1xPBS and 1 μL 200-nm crimson beads (1:106 diluted from custom-designed beads in deionized water, Invitrogen) were added near the outer edge region of the visual cortex ...
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bioRxiv - Neuroscience 2024Quote: ... The DNA precipitate was washed three times in DNA-washing solution (70% ethanol [v/v] and 0.1 M NaCl) and transferred to 500 μl DNase-and RNase-free water (Gibco and Invitrogen, respectively). The DNA was quantified ...
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bioRxiv - Immunology 2024Quote: Frozen PBMCs were thawed in a water bath and resuspended in 5 mL of prewarmed complete RPMI medium (RPMI 1640 [Gibco] supplemented with 10% FBS [Gibco] ...
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bioRxiv - Immunology 2024Quote: Frozen PBMCs were thawed in a water bath and resuspended in 5 mL of prewarmed complete RPMI medium (RPMI 1640 [Gibco] supplemented with 10% FBS [Gibco] ...
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bioRxiv - Neuroscience 2024Quote: ... Following rapid decapitation CTX and HIP tissue were dissected and placed in dissection media (water, 1x HBSS, 1% 1 M HEPES, 1% penicillin/streptomycin (Gibco; Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The dried peptides were redissolved in buffer A (0.1% formic acid in water) and separated by EasyLC 1000 nanoflow-HPLC system (ThermoFisher Scientific) with a 20 cm fused silica column with an inner diameter of 75 μm and in-house packed with C18 (ReproSil-Pur 120 C18-AQ ...
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bioRxiv - Microbiology 2024Quote: ... one inoculation loop with bacterial colony material per isolate was transferred to sterile nuclease-free water (Thermo Fisher Scientific, USA) and incubated at 100 °C for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was diluted 1:5 in RNase-free water and mixed with the PowerUP SYBR Green Master mix (Applied Biosystems) per protocol instruction and analyzed on a QuantStudio 6 Flex (Applied Biosystems) ...
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bioRxiv - Bioengineering 2024Quote: ... Unbound antibodies were rinsed using PBS and DI water and mounted using the ProLong™ Diamond antifade mounting reagent (ThermoFisher). Fluorescence was observed using an Olympus Fluoview FV3000 Confocal Laser Scanning Microscope (Tokyo ...
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bioRxiv - Molecular Biology 2024Quote: ... Sections were then washed in flowing tap water for 10’ and in Dulbecco’s phosphate buffered saline + CaCl2 + MgCl2 (Gibco, DPBS). Sections were blocked in 10% normal goat serum (NGS ...
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bioRxiv - Biochemistry 2024Quote: ... Stocks of fluorescent RNAs intended for single-molecule experiments were prepared by resuspending the lyophilized oligonucleotides in nuclease-free water (20-50 µM, Ambion). RNA concentrations were calculated from their absorbance values 260nm using a NanoDrop and the extinction coefficients from IDT via the Beer-Lambert law.
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bioRxiv - Biochemistry 2024Quote: ... The resulting peptides were desalted using Sep-Pak C18 cartridges (Waters, MA, USA) and dried in a SpeedVac (Thermo Scientific).
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bioRxiv - Neuroscience 2024Quote: ... pellets were left to dry completely and resuspended in 25 μl of nuclease/RNase-free water (Thermo Fisher Scientific, USA). The concentration of RNA in each sample was measured using a Nanodrop Spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... RNA samples were eluted in 20μL of nuclease-free water and reverse transcribed to cDNA using a High-Capacity cDNA Reverse Transcription Kit (Thermo Scientific, product no ...
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bioRxiv - Immunology 2024Quote: ... Dried pellets were dissolved in sterile nuclease free water and DNA concentration was measured on a Nanodrop 1000 (Thermo Scientific). Amplification of sgRNAs cassettes from the extracted DNA was performed using Illumina compatible primers from IDT as described previously23 with some minor modifications ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... The final RNA pellet was washed with 70% ethanol followed by air drying for 2 hrs followed by dissolving in nuclease free water (Invitrogen). RNA cleanups were performed using TURBO DNase (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... Precipitated cRNA was dissolved in RNase-free water and its concentration was measured with a Nanodrop spectrophotometer (ThermoFisher Scientific, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... The RNAs captured in the column were washed twice with 80% ethanol and eluted in 20 µL of UltraPure distilled water (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... 0.01% skim milk in distilled water) and incubated with Alexa Fluor 488-conjugated anti-rat IgG secondary antibody (Cat. No A11006, 1:400, Invitrogen,) and Alexa Fluor 555-conjugated anti-rabbit IgG secondary antibody (Cat ...
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bioRxiv - Immunology 2024Quote: ... Coverslips were washed twice with 1x PBS and twice with deionized water and mounted on glass slides using Prolong Gold Antifade Reagent (Invitrogen)
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CRISPR-based environmental biosurveillance assisted via artificial intelligence design of guide-RNAsbioRxiv - Molecular Biology 2024Quote: ... 2 µL of 500 nM LwaCas13a (GenScript, #Z03486; diluted in RNAse-free water and 1X RNAse-free PBS pH 7.4, Invitrogen, #AM9624), 1 µL of 1 µM crRNA (GenScript ...
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bioRxiv - Molecular Biology 2024Quote: ... were washed with PBS twice and scraped on ice into a 200 μL solution of 25 mM Ammonium Bicarbonate (AmBic) in LC-MS grade water (ThermoFisher), 0.15% benzoase nuclease (Millipore ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were prepared on agar pads (1% agarose in Milli-Q water, poured into 1.0 × 1.0 cm GeneFrames; Thermo Fisher Scientific). Cells were illuminated with a 488 nm laser at 5% intensity and a 30 ms exposure time in Lattice-SIM mode ...
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bioRxiv - Neuroscience 2024Quote: ... was added to PCR master mix (18µL, Qiagen 201203: taq polymerase, DNAse and RNAse- free water, 10X buffer, 10mM dNTPs, and custom DNA oligo primers [ThermoFisher: Forward-TGGTAGTCACATTGCCTCTG ...
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bioRxiv - Bioengineering 2024Quote: ... was then carried out to concentrate the mRNA the 2 mg/mL and to exchange the mRNA into UltraPureTM DNase/RNase-Free Distilled Water (Invitrogen).
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bioRxiv - Biochemistry 2024Quote: After desalting all samples were run on a nanoACQUITY UltraPerformance liquid chromatography system (Waters, Elstree, UK) coupled offline to an Orbitrap Elite analyser (ThermoFisher). Peptides were separated on a bridged ethyl hybrid C18 analytical column (250 mm length ...
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bioRxiv - Genetics 2024Quote: ... After washing the PEI solution with water the PhenoPlates were coated with 10 µg/ml laminin solution (Thermo Fisher, #23017015). For the QC the cells were plated on Cultrex coated Corning™Costar™96 well plates (R&D systems ...
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bioRxiv - Genomics 2024Quote: ... The experimental tanks were placed into four steel water baths that maintained target temperatures with a deviation of less than 0.1°C (model Haake SWB25, Thermo Scientific). A further 1 L of filtered sea water (FSW ...
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bioRxiv - Bioengineering 2024Quote: ... Phase separation was induced by adding 188 µL water with 0.1% ammonium formate (Thermo Fisher Scientific Inc., Waltham, MA, US). The extract was centrifuged at 10,000 g for 5 min ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were washed through a series of 5xSSCT washes (diluted in nuclease-free water from 20xSSCT, Thermo Fisher Scientific, 15557044) in the dark at room temperature (2x 5 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... Tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 µm resin (Waters) on an in-line 150 x 0.075 mm column using an UltiMate 3000 RSLCnano system (Thermo Fisher). Peptides were eluted using a 60-min gradient from 98:2 to 65:35 buffer A:B ratio (Buffer A = 0.1% formic acid ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA pellet was taken up into ultra-pure water and its concentration was measured on a NanoDrop (Thermo Scientific).
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bioRxiv - Cell Biology 2024Quote: ... and once in Milli-Q water and were then mounted in Mowiol 4-88 containing TetraSpeck™ Microspheres 0.5μm (Invitrogen T7281) at 1/200.
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bioRxiv - Cell Biology 2024Quote: ... pH 9.5) and loaded onto an Acquity UPLCTMM-Class CSHTMC18 column (Waters) using a Dionex Ultimate 3000 HPLC system (Thermo scientific). Approximately 100 μg of the non-modified peptide samples was fractionated ...
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bioRxiv - Biochemistry 2024Quote: ... the dried metabolite extract was re-suspended in 50 µl of LC-MS-grade water (Optima-Grade, Thermo Fisher Scientific). After 15 minutes incubation on a thermomixer at 4 °C and a 5 minutes centrifugation at 16,000 x g at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... were loaded on an XBridge Protein BEH C4 column (2.5 µm particle size, dimensions 2.1 mm × 150 mm; Waters) using a VanquishTM Horizon UHPLC System (Thermo Fisher Scientific) with a working temperature of 50◦C ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was washed and eluted using 100 µl nuclease free water and quantification carried out with a NanoDrop UV-Vis Spectrophotometer (ThermoFisher).
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bioRxiv - Biochemistry 2024Quote: ... tryptic peptides were separated by reverse phase XSelect CSH C18 2.5 um resin (Waters) on an in-line 150 × 0.075 mm column using an UltiMate 3000 RSLCnano system (ThermoFisher Inc.). Peptides were eluted using a 60 min gradient from 98:2 to 40:60 solvent A:B ratio (solvent A = 0.1% formic acid ...
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bioRxiv - Biochemistry 2024Quote: ... cells were re-stimulated for 30 min with 1× water-solubilized mix of essential (cat. no.11130036, Thermo Fisher Scientific) and non-essential (cat ...
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bioRxiv - Bioengineering 2024Quote: ... was diluted with deionized (DI) water at a 60% volume ratio and sterile filtered (1.0 µm, Target2™, Thermo Scientific) for all experiments ...
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bioRxiv - Bioengineering 2024Quote: ... was then carried out to concentrate the mRNA the 2 mg/mL and to exchange the mRNA into UltraPureTM DNase/RNase-Free Distilled Water (Invitrogen).
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bioRxiv - Cell Biology 2024Quote: ... and the pellet was resuspended in 3ml of homogenization buffer(HB: 5mM Tris, 250 mM Sucrose, 1mM EGTA in mass-spectrometry grade water (ThermoFisher), pH=7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated and collected by centrifugation before final elution with DNase/RNase-free distilled water (Thermo Scientific, 10977-015). RNA sequencing was then performed by Azenta Life Sciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Slide humidity chambers were prepared by placing dish sponges saturated with Milli-Q water (MicroPure UV/UF, Thermo Scientific, 50132370) in the bottom of airtight plastic containers ...
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bioRxiv - Cell Biology 2024Quote: ... Pellets were suspended in 31 μl of nuclease-free water and quantified using Qubit 2.0 fluorometer with Qubit dsDNA HS Assay Kits (Invitrogen, Q32854). The libraries were then prepared using the NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... of the tryptic digest (in 2 % acetonitrile/0.1% formic acid in water) was analyzed by LC/MS/MS on an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Scientific) interfaced with a Dionex UltiMate 3000 Binary RSLCnano System ...
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bioRxiv - Developmental Biology 2024Quote: ... before being finally resuspended in 1µl of water and transferred to individual wells of a 384 well plate (ThermoFisher, AB1384), on a cold block ...
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bioRxiv - Cell Biology 2024Quote: ... the growth medium was diluted down to 75% of its original concentration with ultrapure DNase/RNase-free distilled water (Invitrogen). For the isosmotic control ...