Labshake search
Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Bioengineering 2024Quote: ... and RSV B/ 18537 DS-Cav1 (51) constructs were expressed by transient transfection of FreeStyle 293-F cells (Thermo Fisher) with polyethylenimine (PEI ...
-
bioRxiv - Cancer Biology 2024Quote: ... Protein was produced from IL-2 and IL-12 expressing stable lines during one-week culture in serum-free media (Freestyle 293, Invitrogen) and purified with HisPur Ni-NTA affinity resin (ThermoFisher Scientific) ...
-
bioRxiv - Biochemistry 2024Quote: ... Stable cell lines for expression were generated using the doxycycline-inducible Flp-In T-Rex 293 system (Thermo Fisher Scientific) according to the manufacturer’s guidelines.
-
bioRxiv - Biochemistry 2024Quote: ... EXPI293 cells were diluted to 0.5×105 cells per millilitre 48 hours prior to transfection with FreeStyle 293 Expression Medium (ThermoFisher Scientific). Cells counts were adjusted to 1.5×106 cells/mL immediately prior to transfection ...
-
bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were transfected with pcDNA4TO HMM-HA-Spike and pcDNA tagBFP for transfection marker using ExpiFectamine 293 (Thermo Fisher Scientific). Cells were centrifuged at 200 g for 2 min 48 h after transfection and washed with Dulbecco’s phosphate buffered saline (PBS ...
-
bioRxiv - Microbiology 2023Quote: ... with a secretion sequence and C-terminal His6-tag) were transiently expressed using FreeStyle™ 293-F cells (Thermo Fisher) in FreeStyle™ F17 Expression Medium supplemented with L-glutamine and 1X MEM non-essential amino acids (Gibco) ...
-
bioRxiv - Immunology 2023Quote: ... plasmids encoding for various SCTs were co-transfected with a plasmid encoding for an ER-retained BirA-ligase into FreeStyle 293-F cells (Invitrogen) using the 293-free transfection reagent (Merck ...
-
bioRxiv - Cell Biology 2023Quote: ... Y-A or YIY-AAA) and FLAG-tagged CDK-1 constructs were expressed in FreeStyle 293-F cells (ThermoFisher Scientific). Cells were transfected using FreeStyle MAX Reagent and OptiPRO SFM according to the manufacturer’s guidelines (ThermoFisher Scientific) ...
-
bioRxiv - Molecular Biology 2023Quote: ... were transfected with expression vectors for 3xFLAG-tagged wt eIF3g or its mutated forms using Expifectamine 293 according to the manufacturer’s protocol (Thermo Scientific). Cells expressing 3xFLAG-tagged eIF3g (200 ml suspension ...
-
bioRxiv - Biochemistry 2023Quote: The WDR76 knockout cell line was derived from Flp-In™-293 Cell Line purchased from Invitrogen (Carlsbad, CA, USA). The knockout was achieved using RNA-guided CRISPR (Clustered regularly interspaced short palindromic repeats)-Cas9 system (51) ...
-
bioRxiv - Immunology 2023Quote: The cells used for expression of the rIgE clones construct were Freestyle 293-F cells (Thermofisher, cat no: K900001/510029). A 1 mL stock of 1*10^7 cells were rapidly thawed from −80°C in 37°C water bath and transferred to 19 mL prewarmed Freestyle™ 293 Expression Medium (Thermofisher ...
-
bioRxiv - Biochemistry 2022Quote: HEK293F cells were cultured in serum-free FreeStyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher Scientific). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
-
bioRxiv - Biochemistry 2022Quote: Murine neuroblastoma Neuro-2a cells (#CCL-131; ATCC, Manassas, VA, USA) and Flp-In T-REx 293 cells (Thermo Fisher) were maintained in DMEM (D5796 ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids encoding heavy and light chains were transfected into Expi293F cells in a 2:1 mass ratio using an ExpiFectamine 293 transfection kit (Gibco). Six days post-transfection ...
-
bioRxiv - Immunology 2023Quote: ... The full-length spike proteins were stabilized by introducing 2 prolines at amino acid positions 986 and 987 (Spike 2P) and transiently transfected in FreeStyle 293-F cells using Turbo293 (SpeedBiosystems) or 293Fectin (ThermoFisher). The constructs contained an HRV3C-cleavage site followed by His and/or streptagII tags for purification purposes ...
-
bioRxiv - Neuroscience 2023Quote: ... 7 x 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM (# 41965062, Thermo Fisher Scientific) containing 10% fetal bovine serum (#16000-044 ...
-
bioRxiv - Biochemistry 2023Quote: Expression and purification of secreted SIDT1 and SIDT2 extracellular domain The SIDT1ECD and SIDT2ECD proteins were expressed using FreeStyle 293-F cells (Invitrogen). The cells were cultured in OPM-293 CD05 medium (OPM Biosciences co. ...
-
bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- suspension cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
-
bioRxiv - Microbiology 2023Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
-
bioRxiv - Biophysics 2024Quote: ... HEK-293 cells were grown to 40%-50% confluency in DMEM (GenDEPOT) supplemented with 10% FBS (GenDEPOT) and penicillin/streptomycin (Invitrogen) and transfected with GluA2(Q) ...
-
bioRxiv - Molecular Biology 2024Quote: ... and human embryonic kidney cell line HEK293 Flp-In T-Rex cells (Cellosaurus Flp-In-T-REx-293 (CVCL_U427)) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (GibcoTM, Thermo Fisher Scientific) including 10% FBS ...
-
bioRxiv - Immunology 2024Quote: ... Plasmids encoding antibody heavy and light chain genes were used to transfect suspension Expi293F cells in a 1:2 heavy to light chain ratio using ExpiFectamine 293 transfection reagents (Gibco) according to the manufacturer’s instructions ...
-
Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... ExpiCHO cells were transiently transfected with the vector containing the gene encoding soluble spike glycoprotein ectodomain or CR3022 (using a heavy chain:light chain ratio of 1:2) using the Expifectamine 293 Transfection Kit (ThermoFisher #A29129), following the user manual ...
-
bioRxiv - Microbiology 2024Quote: ... including a C-terminal His6-tag and transfected into mycoplasma screened Expi293F™ GnTI- cells using ExpiFectamine 293 (ThermoFisher Scientific). Mutagenesis of RBK21 constructs was carried out using the Q5 Site Directed Mutagenesis kit (New England Biolabs ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... alecto EFNB2 and EFNB3 were expressed in Expi293F cells by transient transfection using the ExpiFectamine 293 Transfection Kit (Thermo Fisher) according to the manufacturer’s protocols ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2ug of CBP-pRetroX-pTRE3G constructs or pRetroX-Tet3G were co-transfected with 2ug of the envelope pVSV-G plasmid into GP2-293 cells using lipofectamine 3000 (ThermoFisher). After 48 hours ...
-
bioRxiv - Developmental Biology 2022Quote: ... 3% FBS (Gibco), 1% MEM non-essential amino acids (Gibco) ...
-
bioRxiv - Genomics 2019Quote: ... 3 (Applied Biosystems).
-
bioRxiv - Microbiology 2021Quote: ... DiOC2(3) (Invitrogen) was added to a final concentration of 30µM or DiSC3(5 ...
-
bioRxiv - Cancer Biology 2022Quote: ... 3% FBS (Invitrogen), 20 ng ml−1 EGF (Peprotech ...
-
bioRxiv - Cell Biology 2020Quote: ... SUMO2/3 (Invitrogen), β-catenin (BD Transduction Laboratories) ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... 3 (Applied Biosystems). We assembled forward and reverse reads using the Geneious (https://www.geneious.com ...
-
bioRxiv - Neuroscience 2023Quote: ... 3% FBS (Gibco), 0.1mM MEM-NEAA (Gibco) ...
-
bioRxiv - Immunology 2024Quote: ... TOPRO-3 (Invitrogen) was added to the staining buffer at a dilution of 1:10000 ...
-
bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
-
bioRxiv - Immunology 2021Quote: ... as well as the human lung epithelial Calu-3 cells (ATCC® HTB-55™) were maintained in Dulbecco modified Eagle medium (Gibco Life Technologies, Gaithersburg, Md.) containing 10% fetal calf serum (FCS) ...
-
bioRxiv - Cell Biology 2022Quote: Transient genetic silencing of HOIP and FLOT1/2 was performed by reverse transfection of HT-29 cells with the following siRNAs: human siHOIP (RNF31) (Silencer® Select siRNA #1 s30108, #2 s30109, #3 s30110, 40 nM) (Thermo Fisher Scientific, Waltham, MA, USA), human FLOT1 (ON-TARGETplus Human FLOT1 siRNA SMART POOL ...
-
bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
-
bioRxiv - Developmental Biology 2020Quote: ... grown in 12-well plates in DMEM supplemented with 10% FCS (Gibco-BRL) and glutamine ...
-
bioRxiv - Microbiology 2022Quote: ... Nb fused with Fc was purified using Protein G (cat.# 20399, Thermo Scientific).
-
bioRxiv - Molecular Biology 2021Quote: ... Opti-MEM and fetal calf serum (FCS) were from GIBCO (Waltham, MA, USA). All cell culture ware was purchased from BD Falcon (Corning ...
-
bioRxiv - Immunology 2019Quote: ... supplemented with 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific), 50µM β-mercaptoethanol (Sigma) ...
-
bioRxiv - Immunology 2019Quote: ... Cell pellets were resuspended in FACS buffer (5% FCS in PBS, Life Technologies). After adding Fcγ receptor block (1:100 dilution ...
-
bioRxiv - Genomics 2019Quote: A375 cells were grown in DMEM + 10% FCS + Penicillin/streptomycin (Gibco, Carlsbad, MA) and were treated with 10,000 J/m2 UVB 310 nm (7 mins @ 25 J/m2/s ...
-
bioRxiv - Genetics 2020Quote: ... with a final quench in PBS + 2% fetal calf serum (FCS, Invitrogen 26140079). Cells were permeabilized for 5 minutes with saponin buffer (PBS + 0.2% Saponin (Sigma S7900 ...
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific).
-
bioRxiv - Bioengineering 2021Quote: ... supplemented with 10% fetal calf serum (FCS) and 1% PenStrep (Thermo Fisher Scientific). Imaging was performed in a heating chamber preheated to 37 °C and placed on an inverted microscope (IX83 from Olympus ...
-
bioRxiv - Systems Biology 2020Quote: ... PHHs were cultivated in Williams medium E (Biochrom) supplemented with 10% FCS (Gibco), 100 nM dexamethasone ...
-
bioRxiv - Molecular Biology 2021Quote: ... All media were supplemented with 10% fetal bovine serum (FCS, Biochrom or GIBCO), 100 U/ml of penicillin and 0.1 mg/ml of streptomycin (PAN-Biotech) ...