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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Human embryonic kidney 293T cells (HEK 293T) (Pear et al., 1993) were maintained in Dulbecco’s modified Eagle medium (DMEM; ThermoFisher Scientific) supplemented with FBS ...
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bioRxiv - Immunology 2019Quote: ... Fabs and IgGs were produced by transient transfection in suspension 293F or adherent HEK 293T cells using Lipofecatamine 2000 (Invitrogen) or polyethylenamine (PEI) ...
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bioRxiv - Immunology 2021Quote: Human embryonic kidney (HEK) 293T cells were acquired from ATCC and cultured in DMEM media supplemented with 10% fetal bovine serum (FBS; Gibco) and 100 U/mL of penicillin and 100 μg/mL streptomycin (Gibco) ...
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bioRxiv - Zoology 2022Quote: HEK 293T cells were a gift from Kristin Dittenhaffer-Reed (Hope College, USA) and grown in DMEM (ThermoFisher Sci. #11965092) with 10% FBS (ThermoFisher Sci ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant containing lentivirus was generated by seeding 3.8 x 105 cells x ml-1 HEK 293FT cells (Invitrogen R700-07) in 10 cm tissue culture plates (10 ml per plate ...
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bioRxiv - Neuroscience 2024Quote: Chinese Hamster Ovary (CHO) cells were maintained similar to HEK cells with the following differences: Culture media was IMDM (Invitrogen) supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2023Quote: For expression analysis of truncated WDR47 constructs HEK cells were transfected with different HA tagged Wdr47 constructs using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK-293T cells were transfected with shRNA plasmid and helper plasmids psPAX2 and pMD2.G using Lipofectamine 2000 (Invitrogen #11668027) in Opti-MEM (Invitrogen #22600-050 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 2nd generation lentiviral packaging plasmids into HEK 293T cells at 60-80% confluency with 60uL Lipofectamine3000 (Thermo Fisher Scientific; L3000008) and 50uL P3000 reagent (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Blue Native PAGE was performed using lysates prepared from growing HEK-293T cells according to the manufacturer’s protocol (Thermo Fisher). 1ξ107 cells were collected and lysed using 1x Native PAGE lysis buffer including protease inhibitor cocktail and 1% digitonin ...
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bioRxiv - Neuroscience 2023Quote: ... Media in 10 cm TC dishes with HEK 293T cells (at around 70% confluence) were replaced with Opti-MEM (Invitrogen). Then the SSF-MOR plasmid ...
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bioRxiv - Immunology 2023Quote: ... the signal was evaluated after 48 hours by detection of GFP expression in the HEK-293T-hACE2 cells using Attune NxT Acoustic Focusing Cytometer (ThermoFisher) or BD Symphony Flow Cytometry.
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bioRxiv - Genomics 2023Quote: The the HEKs and the HDFs were separated and homogenized in 500 µL of TRIzol reagent (Invitrogen, Carlsbad, CA, USA) using a microhomogenizer following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: pRT074 was lentivirally packaged in HEK 293T cells (ATCC Cat. No. CRL-3216) DMEM complete medium: DMEM (Gibco, 11965-092) supplemented with 10% FBS (VWR ...
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bioRxiv - Microbiology 2023Quote: Approximately 8·106 HEK-293T cells were seeded into T75 flasks previously coated with poly-D-lysine (Gibco™ A3890401). After 16 - 24 h (when cells reached around 90% confluence) ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T and HuTu-80 (CLS, 300218) cell lines were expanded in growth medium (DMEM or DMEM:F12, respectively; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: The human embryonic kidney 293EBNA1-6E cell line (HEK 293EBNA1-6E) was adapted to growth in suspension in F17 medium (FreeStyleTM F17 Expression Medium, Gibco), supplemented with 4 mM L-Glutamine (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: Lentivirus-compatible expression vectors generated from Gateway Cloning were transfected in HEK-293T cells with Lipofectamine 3000 Transfection Reagent (cat. no. L3000001, Invitrogen) along with psPAX2 and pVSV-G viral packaging plasmids (Addgene ...
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bioRxiv - Cancer Biology 2019Quote: ... Real time PCR analysis was performed on 1µL of cDNA using TaqMan Gene Expression Master Mix according to the manufacture’s procedures using FAM labeled TaqMan® and PrimePCR gene expression human primer/probe sets (Thermo Scientific and BioRab; see Supplementary Table 3). mRNA expression was compared to untreated control using the ΔCT method ...
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bioRxiv - Immunology 2021Quote: ... and pFUSE2ss-CLIg-hk or pFUSEss-CLIg-hL2 respectively and were co-transfected in equal parts using ExpiFectamine™ 293 Transfection Kit(Gibco) according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... The expression plasmids encoding HC and LC sequences were co-transfected into Expi293F cells using ExpiFectamine™ 293 Transfection reagents (Gibco). For IgG expression ...
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bioRxiv - Neuroscience 2019Quote: ... 7 × 106 AAV-293 cells were seeded into 150 mm cell culture plates in DMEM containing 10 % fetal bovine serum (ThermoFisher, USA) and penicillin/streptomycin ...
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bioRxiv - Biochemistry 2020Quote: ... HEK293F cells were cultured at 37 °C with 6% CO2 and 70% humidity in Free Style 293 medium (Thermo Fisher Scientific) supplemented with 1% fetal bovine serum (FBS).
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bioRxiv - Immunology 2019Quote: ... the transfection mix was added to 1L cells at a density of ~1.2×106 cells/ml in FreeStyle 293 Expression Medium (Thermo Fisher - 12338018). The cells were incubated at 37°C and 8% CO2 for 6 days ...
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bioRxiv - Immunology 2021Quote: ... containing additional S383C and D985C substitutions (Henderson et al., 2020) with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Microbiology 2020Quote: One 96-well plate was coated with 200ng/well of recombinant N protein and another plate was coated with 200ng/well of recombinant 6xHis-tagged SARS-CoV-2 spike protein receptor binding domain (RBD) produced using the FreeStyle 293 Expression System (Thermo Fisher) from BEI Resources (Cat# NR-52366 ...
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bioRxiv - Biochemistry 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... Expi293F™ cells were grown in Expi293™ media and transiently transfected using ExpiFectamine™293 (all from Thermo Fisher Scientific).
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 HexaPro Spike protein for cryoEM analysis was produced in HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life Technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...
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bioRxiv - Biophysics 2020Quote: Stable inducible Flp-In T-Rex-293 cell line expressing SERCA2a was generated using the Flp-In T-REx Core Kit (Invitrogen, USA) as described (28) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... These vectors were either transiently transfected or stably transfected into Flp-In™ 293 cells using Lipofectamine LTX (Thermo Fisher Scientific). 500 ng of DNA and 1 µL of Lipofectamine LTX or 10 µg of DNA and 44 µL of Lipofectamine LTX were used for transfection into each well of the 24-well plate (seeding density 2.0×105 cells/well ...
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bioRxiv - Immunology 2020Quote: ... Expi293F™ cells were grown in Expi293™ media and transiently transfected using ExpiFectamine™293 (all from Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2021Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...
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bioRxiv - Biochemistry 2021Quote: ... 293 cells from a pMLINK tetracistronic vector (courtesy of Y. Shi, Tsinghua University, Beijing).45 HEK293 cells were cultured in Freestyle 293 media (Life Technologies), shaking at 125 rpm while incubating at 37 oC with 8% CO2 until a density 2 × 106 cells/ml was reached ...
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bioRxiv - Biochemistry 2021Quote: ... MERS-CoV S2-apex-less (MERS-CoV S2-only construct with residues 811–824 replaced with GGSGGS and residues 1042–1073 replaced with a flexible linker) were expressed in Freestyle 293-F cells (ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... spike protein was expressed by transiently transfecting plasmid encoding the HexaPro spike variant2 containing substitutions S383C and D985C3 with a C-terminal TwinStrep tag into FreeStyle 293-F cells (Thermo Fisher) using polyethyleneimine ...
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bioRxiv - Immunology 2020Quote: ... and 1 μg DNA per 1 mL cell culture medium at a cell density of 0.8 106 cells/mL in FreeStyle 293 medium (Thermo Fisher Scientific). After 7 days of culture at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: RBD-Fc and RBD-6xHis proteins were produced by transient transfection of Expi293F high-density cells with ExpiFectamine 293 Lipid Cation Transfection Reagent (Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: HEK293T and Flp-In T-REx 293 cells were maintained in 37°C and 5% CO2 in Dulbecco’s Modified Eagle Medium (Thermo Fisher, 41966029) supplemented with 10% Fetal Bovine Serum (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: Flp-In T-REx 293 cells were co-transfected with pcDNA5/FRT/TO and pOG44 plasmids using Lipofectamine2000 (Thermo Fisher, 11668019). 24h after transfection ...
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bioRxiv - Molecular Biology 2021Quote: ... with the respective plasmids using the protocol provided with the FreeStyle 293 Expression System (Thermo Fisher Scientific, Cat. no. K9000-01). The isotypes contain human V regions from hybridomas that were established from a human HHKKLL Trianni mouse (Patent US 2013/0219535 A1) ...
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bioRxiv - Microbiology 2022Quote: ... Expi293 cells were transfected with the expression plasmid hACE2-1-740aa-8xHis or hACE2-1-614aa-8xHis using ExpiFectamine 293 (ThermoFisher Scientific). Four days later ...
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bioRxiv - Cell Biology 2022Quote: The trimeric S-protein and its mutants were produced by transient transfection of Expi293F high-density cells with the ExpiFectamine 293 (Thermo Fisher) lipid cationic transfection reagent according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: A construct encoding for CDC-20::3xFLAG under a CMV promoter was transfected into Freestyle 293-F cells (Thermo Fisher Scientific). 48 hours later ...
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bioRxiv - Cell Biology 2022Quote: ... over the course of 10 days the FBS-supplemented DMEM was serially diluted with FreeStyle 293 Expression Medium (ThermoFisher Scientific, USA). Once growing in 100% FreeStyle Medium ...
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bioRxiv - Immunology 2022Quote: ... Recombinant antibodies were produced by cotransfecting paired heavy and light chain expression plasmids into Expi293F cells using ExpiFectamine 293 transfection reagents (ThermoFisher Scientific), purified from culture supernatants using the Protein A/Protein G GraviTrap kit (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: ... Cell surface display DNA constructs for the SARS-CoV-2 spike variants together with a plasmid expressing blue fluorescent protein (BFP) were transiently transfected into Expi293F cells using ExpiFectamine 293 reagent (ThermoFisher Scientific) per manufacturer’s instruction ...