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Citations for Thermo Fisher :
3101 - 3150 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... This PCR fragment was TOPO cloned into pcDNA™3.1/V5-His backbone (Invitrogen, V81020). We used in vivo assembly cloning 40,41 for site directed mutagenesis to modify the nucleotide 1 bp upstream of the exon 49 splice junction to each of the alternative nucleotides (Supplementary table 1) ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Immunology 2023Quote: ... Glycan samples were run with a LIZ 600 DNA ladder in Hi-Di formamide (ThermoFisher) on an ABI 3500xL DNA sequencer and analyzed with GlycanAssure Data Acquisition Software v.1.0 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged LCRIS_00661 and LCRIS_00558 genes with the appropriate restriction sites were ordered from Invitrogen and subcloned into a previously constructed S ...
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bioRxiv - Microbiology 2024Quote: Dynabeads™ His-Tag Isolation and Pulldown magnetic bead solution (Thermo Fisher Scientific, United States) kit was used for the detection of the binding partner of OipA ...
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bioRxiv - Cell Biology 2024Quote: ... Western blotting was performed using an anti-His tag monoclonal rabbit antibody (Thermo Fisher Scientific) and GST monoclonal mouse antibody (own antibody) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PVDF membrane was then incubated with anti-his mouse primary antibody (Thermo Scientific Pierce) in TBS-T (1:5000 ...
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bioRxiv - Developmental Biology 2023Quote: ... We also subcloned this cassette into a pAc5.1/V5-His A plasmid (Invitrogen #V4110-20) by cutting the cassette out of pUASP-attb-Cry2-CTEV-mCherry using NotI and XbaI (NEB #R3189S and #R0145S ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... and cryopreserved in heat-inactivated fetal-bovine serum (HI-FBS, ThermoFisher Gibco™ #12484028, USA) + 10% (v/v ...
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bioRxiv - Immunology 2023Quote: ... The pIgR gene was inserted into the pEF-Myc-His vector (ThermoFisher Scientific, MS, USA).
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bioRxiv - Neuroscience 2023Quote: ... rattus Piccolo (2622-2937) DNA was cloned into vector pCDNA3.1-myc-his(C) vector (Invitrogen).
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bioRxiv - Molecular Biology 2023Quote: ... One microliter of sample was diluted in 9 ul Hi-Di™ Formamide (Applied Biosystems) containing 0.25 ul GeneScan™ 600 LIZ™ Size Standard ...
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bioRxiv - Microbiology 2022Quote: ... Then 1 μl of Dynabeads™ His-Tag Isolation and Pulldown magnetic beads (ThermoFisher Scientific) were added into each well ...
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bioRxiv - Microbiology 2022Quote: ... 0.02% Tween-20) and added to 20 μl Dynabead His-Tag beads (ThermoFisher Scientific; 10103D). After incubation on a roller at 4°C for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... VarG-HIS bands were excised and diluted with NuPAGE LDS sample buDer (Invitrogen, Carlsbad, CA) mixed with10 mM dithiotreitol (DTT ...
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bioRxiv - Biochemistry 2022Quote: ... each with an N-terminal (His)6 tag from plasmids generated by GeneArt (ThermoFisher Scientific). For each purification ...
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bioRxiv - Biophysics 2023Quote: ... His-tag purification was performed in Pierce disposable polypropylene 5mL disposable columns (Thermo Fisher Scientific) with a wash solution containing TNi 100/300/20 (100 mM Tris-HCl pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... The coding region for Hsc70-4 (CG4264) was cloned into pAc5.1/V5-His A (Invitrogen), and into pcDNA™6/myc-His A (Invitrogen) ...
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A crucial role for dynamic expression of components encoding the negative arm of the circadian clockbioRxiv - Biochemistry 2023Quote: ... with the Ion PI™ Hi-Q™ Chef Kit (Thermo Fisher Scientific, Catalog # A27198) and Ion PI™ Chip Kit v3 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... or F-vec were cultured in DMEM + 10% HI FBS with Zeocin (Thermo Fisher Scientific) selection ...
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bioRxiv - Immunology 2024Quote: ... Amplified PCR fragments were inserted into the metallothionein promoter driven pMT-V5-His vector (Invitrogen). The generated vector was co-transfected with a pCoBlast blasticidin resistance vector with a ratio of 19:1 and cells were selected in blasticidin containing medium according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR product was mixed with Hi-DiTM mix (Thermo Fisher Applied BiosystemsTM, Carlsbad, USA) with GeneScantm LIZ dye Size standardtm (Thermo Fisher Applied BiosystemsTM ...
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bioRxiv - Plant Biology 2024Quote: 12xHis-LIFERING was purified using Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Scientific). 230 µl ubiquitination reaction mixture was added to 15 µl beads equilibrated and resuspended in 230 µl 2X B/W buffer (100 mM sodium phosphate pH 8 ...
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bioRxiv - Molecular Biology 2024Quote: ... immunoblot detection was performed using Anti-His (C-term)-HRP Antibody (Invitrogen, Carlsbad, CA, USA). Bound antibodies were detected using the Amersham ECL Western Blotting Detection Reagent (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The METTL3 and METTL14 sequences were constructed into the pAC5.1b/V5/His expression vector (Invitrogen). The primers used to construct vectors are shown in Table S4 ...
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bioRxiv - Immunology 2024Quote: ... followed by removal of remaining His-tagged Fabs with HisPur Ni-NTA resin (Thermo Scientific). The thrombin-protease was removed via Streptavidin Sepharose High Performance affinity resin (Cytiva) ...
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bioRxiv - Immunology 2024Quote: ... Blot was then incubated with 1:1000 dilution anti-his conjugated to HRP (Thermo Scientific) in 5% milk in 1xTBST (1 h ...
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bioRxiv - Microbiology 2024Quote: ... and the other was treated with 20 U of recombinant Escherichia coli RNase HI (Invitrogen) overnight at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... using the Ion PI™ Hi-Q™ Sequencing 200 Kit (Thermo Fisher Scientific, USA) according to standard protocols.
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Immunology 2022Quote: ... 1.9g of ethyl-3-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC) (ThermoFisher #22980) and 1.2g of N-hydroxysuccinimide (NHS ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Biophysics 2024Quote: ... and 3-pyridinemethanol (RI = 1.545, 3-PM, A10381, Thermo Fisher Scientific) were used to mix with the water-based SMLM buffer ...
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bioRxiv - Genetics 2021Quote: Low-passage HEK 293FT cells were grown in DMEM supplemented with 10% FBS (D10 media) and passaged using TrypLE (Gibco). For viral plasmid transfection ...
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bioRxiv - Immunology 2021Quote: ... the WNV replicon and C-prM-E plasmids were co-transfected into HEK-293T cells using Lipofectamine 3000TM transfection reagent (Invitrogen). Transfected cells were incubated at 30°C and RVP-containing supernatants were harvested 3-6 days post-transfection and pooled ...
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bioRxiv - Neuroscience 2020Quote: ... One fifth of the HEK-T293 culture was split into two petri dishes of 6cm diameter (Falcon) and transfected with lipofectamine (Invitrogen) and 8µg of either pUb6-tdTOM (i.e. ...
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bioRxiv - Bioengineering 2021Quote: ... and human embryonic kidney (HEK 293T) (ATCC, CRL-3216) cell lines were cultured in high-glucose Dulbecco’s Modified Eagle Medium (DMEM; Gibco, 12634010) media supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: ... HTB-96), and HEK-293T (ATCC, CRL-3216) cells were purchased from ATCC (Manassas, VA, USA) and cultured in DMEM (GIBCO, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... together with the helper plasmids pHelper 1.0 (Gag and Pol) and pHelper 2.0 (VSVG) were transiently cotransfected into HEK 293T cells using Lipofectamine 2000 (Invitrogen, USA) to package lentivirus particles ...
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bioRxiv - Neuroscience 2022Quote: HEK cells were transfected with several Mover constructs one day after plating using PEI transfection in Opti-MEM medium (Gibco). Per coverslip ...
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bioRxiv - Cell Biology 2022Quote: Human embryonic kidney 293T (HEK 293T) cells (ATCC CRL-3216) were cultured at 37 °C and 5% CO2 in DMEM (Gibco) supplemented with 4.5 g/L glucose ...
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bioRxiv - Genomics 2020Quote: Live cell images of NIH3T3 cells (Fig. 1B) and HEK 293T cells (Fig. S1B) were taken and digitally recorded using FLoid Cell Imaging Station (ThermoFisher). In the experiments with ActD treatments (Fig ...
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bioRxiv - Bioengineering 2020Quote: ... 2013) and co-transfected with plasmids containing Cas9 effectors into A375 or HEK 293FT cells using Lipofectamine 2000 (ThermoFisher 11668019). The transfected cells were selected with 2 μg ml-1 puromycin for 72 h ...
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bioRxiv - Genomics 2020Quote: HEK 293T and HeLa cells were obtained from ATCC and maintained in DMEM supplemented with 10% FBS (ThermoFisher Scientific, 10082147) and antibiotics at 37°C in 5% CO2.