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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
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bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... the His-tagged protein was purified using the ProBond Purification System (Thermo Fisher Scientific) and the eluted protein was dialyzed against PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were tested on preconfigured 96-well qPCR plates (Human glycosylation – 4413255, Human Inflammation - 4418851 or Human tumor metastasis – 4418743, Thermofisher Scientific), with 100 ng added to each well ...
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bioRxiv - Cancer Biology 2024Quote: Isolated human PBMCs (ATCC PCS-800-011) were activated with human monoclonal antibodies against human CD3 (OKT3, Life Technologies, 16-0037) coated onto a flask and 2 ug/ml CD28 (CD28.2 ...
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bioRxiv - Cell Biology 2020Quote: ... EndoB1 siRNA 5’-UGUUUAUACGACUUGGAGCUU-3’ and 3’-AAGCUCCAAGUCGUAUAAACA-5’ (Invitrogen), control siRNA (Ambion) ...
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bioRxiv - Microbiology 2021Quote: ... FluoZin-3 (FluoZin™-3, AM, cell permeant, Thermo Fisher) was added at 1 mM ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... mounting 3 dpf embryos in 3% methylcellulose (Thermo Scientific, 258111000). After imaging ...
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bioRxiv - Cell Biology 2023Quote: ... siPALS1 (5’-UUCCUUAUGAUGAACUGGCtt-3’) and siPATJ (5’-CCAGAUACUCACACUUCAGtt-3’, Ambion), siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’ ...
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bioRxiv - Biochemistry 2023Quote: ... DiIC12(3) (1,1’-Didodecyl-3,3,3’,3’-Tetramethylindocarbocyanine Perchlorate) (Invitrogen, D383) was used ...
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bioRxiv - Cell Biology 2022Quote: ... Full length Cavin1 and ΔDR1 mutants were expressed and purified from suspension cells HEK-293F (Invitrogen, Carlsbad, CA, USA) as described previously (33) ...
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bioRxiv - Cancer Biology 2020Quote: HEK-293T cells were plated at a density of 1.0 × 106 cells/10 cm plate in RPMI 1640 (Gibco) supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Biochemistry 2019Quote: Human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in Dulbecco’s Modified Eagle Medium (DMEM, high glucose (Gibco)) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: HEK cells were grown in 6 well plates and transfected with 4µg of each DNA with lipofectamine 2000 (Invitrogen) for 6hrs according to manufacturers instructions.
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bioRxiv - Bioengineering 2019Quote: HEK 293T cells (authenticated by Bio-Synthesis, Inc. and tested negative for mycoplasma using a kit from Molecular Probes) were plated at low density on poly-L-lysine coated coverslips (18 mm ...
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bioRxiv - Cancer Biology 2020Quote: RPE1-hTERT and HEK 293T cell lines were purchased from ATCC and cultured in DMEM (41966052; Thermo Fisher Scientific) containing 10% fetal bovine serum (10500064 ...
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bioRxiv - Neuroscience 2020Quote: HEK 293T cells were cultured at 37°C in 5% CO2 in DMEM+ GlutaMax (Thermo Fisher, cat. no. 10566016) with 10% fetal bovine serum (Sigma ...
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bioRxiv - Cell Biology 2021Quote: ... pcRV-Gagpol and pMD2G vectors into HEK-293T cells with lipofectamine 3000 according to manufacturer instructions (Thermo Fisher, LF300001). Target cells were infected with lentivirus supplemented with 5µg/ml polybrene (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... HEK cells were transfected using Lipofectamine 2000 according to different siRNA quantity in Opti-MEM medium (Thermo scientific, USA). The transfected cells were infected with either CHIKV strains PS or IS with MOI 0.1 at 24 hours post transfection (hpt) ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Molecular Biology 2020Quote: HeLa and HEK-293T mammalian cell lines were maintained in RPMI and DMEM-High glucose (Gibco, ThermoFisher Scientific, USA) media ...
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bioRxiv - Immunology 2022Quote: Proteins were expressed by transient transfection of HEK 293F cells (0.8-1.2 million cells/mL) maintained in Freestyle medium (Life Technologies). A 3:1 ratio of PEImax and expression plasmids (312.5 µg/L cells ...
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bioRxiv - Biochemistry 2022Quote: A polyclonal HEK cell line that stably expresses AE1 upon tetracycline induction was generated using the TRex system (Invitrogen). Cells were plated in a 96-well plate and incubated overnight with or without 2 μg/ml tetracycline at 37° C ...
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bioRxiv - Cell Biology 2019Quote: ... C2C12 and Fyn-KD-HEK 293T) were carried out using Lipofectamine 3000 according to the manufacturer’s instruction (Life Technologies). Cells were checked for expression of plasmid of interest after 18 hours post transfection by fluorescence microscopy ...
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bioRxiv - Microbiology 2020Quote: ... 1.5 × 105 HEK-293A cells were seeded in 12-wells plates and transfected using Lipofectamine 2000 (Thermo Fisher Scientific) with 180 ng pcDNA3-DDX3-HA ...
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bioRxiv - Immunology 2021Quote: HEK 293T-hACE2 and Vero E6 cells (RRID:CVCL_0574) were maintained in Dulbecco’s Modified Eagle Medium (DMEM) (Thermo Fisher Scientific) containing 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2021Quote: Plasmids encoding full length SARS-CoV-2 S (GenBank ID: QHD43416.1) with one or more mutation sites were carried into HEK-293T cells using lipofectamine 3000 (ThermoFisher) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2020Quote: ... HEK 293T cells (2.5 × 105 cells/well in 24 well plates) were grown in DMEM (Gibco Cat# 11995-065) with 10% FBS and 1X PSA (100 units/mL penicillin ...
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bioRxiv - Developmental Biology 2021Quote: ... Each required tagged protein expression construct was transfected in HEK 293T cells with Lipofectamine 3000 transfection reagent (L3000150; Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... 70% confluent HEK-293T cells were cultured for 24 h w/o antibiotics and transfected (Lipofectamine, Thermo Fisher Scientific) with lentiviral transfection constructs and packaging plasmids (psPAX2 and pMD2.G ...
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bioRxiv - Synthetic Biology 2023Quote: ... and HEK 293T AAVS1 LP (HEK293T-LP, kind gift from D.M. Fowler) were cultured respectively in RPMI medium 1640 (Gibco) or DMEM medium (Gibco ...
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bioRxiv - Immunology 2022Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: ... and p976 were packaged in HEK-293T cells at a ratio of 2:1:1 by Lipofectamine 2000 (Invitrogen). The ratio of liposome to DNA was 2:1 ...
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bioRxiv - Genetics 2023Quote: Lentiviral plasmids encoding pCMV-OMP25-tagBFP2-EF1a-NEO and pUbC-UCR-2.3- mNeonGreen-EF1a-Puro were transfected into HEK-293T cells with lentiviral packaging plasmids with Lipofectamine 3000 (Invitrogen) according to standard lentiviral production protocols ...
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bioRxiv - Neuroscience 2024Quote: ... HEK 293T cells reaching 40-60% confluency on 35mm culture plates were transiently transfected using Lipofectamine 2000 (Thermo Fisher) 36-48 hours before experiments ...
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bioRxiv - Genomics 2024Quote: 1.5 x 107 HEK 293T cells were transfected in a 15 cm tissue culture dish using Lipofectamine 2000 (Invitrogen) with 14.1 μg Brunello_iT7 plasmid library ...
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bioRxiv - Microbiology 2024Quote: ... Z-23GUINEA-18 and Z-4GUINEA-18 fragments were transfected in HEK-293T cells using Lipofectamine 3,000 (ThermoFisher, France). After 4 days ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Human Embryonic Kidney (HEK)293T cells (ATCC) were grown at 37°C and 5% CO2 in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Bodinco and Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Human SH-SY5Y neural cells and HEK-293T cells were maintained at 37°C with 5% CO2 in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum and penicillin/streptomycin ...
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bioRxiv - Microbiology 2023Quote: ... 5×104 HEK 293T and 2.5×104 MDCK-SIAT1 cells were plated in 12 well plates in growth media (DMEM, Invitrogen #19965-092 ...
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Bioengineering 2023Quote: All cell culture experiments were performed with HEK-293T cells (ATCC, CRL-3216) grown in 10% FBS / 90% Dulbecco’s Modified Eagle Medium (DMEM; Gibco) in a 37°C / 5% CO2 incubator with 100% relative humidity ...
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bioRxiv - Immunology 2023Quote: ... HEK 293T cells and HeLa cells were transfected with plasmids using lipofectamine 3000 according to the manufacturer’s instruction (Invitrogen).
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bioRxiv - Immunology 2023Quote: HEK 293T cells were maintained between 10% and 90% confluency at 37°C/5% CO2 in DMEM (Life Technologies) supplemented with 10% foetal bovine serum (Sigma) ...