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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... IP3R-null HEK293 (HEK-3KO) cells (Alzayady et al., 2016) were maintained in DMEM/F-12 with GlutaMAX (ThermoFisher) supplemented with 10% ...
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bioRxiv - Synthetic Biology 2023Quote: HEK 293T cells were lysed 16 hours post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
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bioRxiv - Microbiology 2023Quote: ... and HEK 293T-hACE2 (BEI Resources, NIAID, NIH, NR-52511) were cultured in Dulbecco’s Modified Eagle Media (DMEM; Gibco) with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1×105 HEK 293T cells were plated on each respective well of a 4 well chamber slide (Thermofisher Scientific) and incubated overnight at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... HEK 293T and MEF cells were cultured at 37°C with 5% CO2 in DMEM/F12 medium (Thermo Fisher Scientific – Life technologies #LTI 11039-047 ...
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bioRxiv - Genomics 2024Quote: ... 5 x 106 HEK 293T cells were seeded in 10-cm dishes in Dulbecco’s minimal essential medium (DMEM) (Gibco) supplemented with 10 % fetal calf serum (FCS ...
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bioRxiv - Microbiology 2020Quote: ... we used human monoclonal antibodies produced recombinantly in human Expi293F cells (Life Technologies) as described before (Fang et al ...
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bioRxiv - Cell Biology 2021Quote: ... anti-human IgG-Alexa647 and anti-human IgG-Alexa568 were obtained from Invitrogen, anti-acetylated tubulin (T7451 ...
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bioRxiv - Cell Biology 2020Quote: ... and human GAPDH and human KIF18A Taqman probes and primers (Thermo Fisher Scientific) were used for reverse transcription and qRT-PCR ...
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bioRxiv - Neuroscience 2020Quote: ... and subjected to human total tau ELISA (human tau: # KHB0042, Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... Suspension HEK293S GnTI− cells expressing dolphin Prestin were grown at 37 °C and 7.8% CO2 in t FreeStyleTM 293 expression medium (Gibco Fisher Scientific) supplemented with 2% heat-inactivated fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2019Quote: GAL4–PAX6 was cloned into the pcDNA5/FRT/TO expression vector by standard procedures and subsequently used for recombination into the genomic DNA of TREx 293 Flp-In cells (Invitrogen) as described in details previously41 ...
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bioRxiv - Molecular Biology 2019Quote: Human embryonic kidney 293 T (HEK293T) cells were maintained in MEM/EBSS (HyClone) supplemented with 10% fetal bovine serum (FBS, Gibco), 100U/ml penicillin and 100μg/ml streptomycin (Lonza) ...
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bioRxiv - Molecular Biology 2019Quote: ... The purified cosmid DNA (2 μg) was digested with Pac1 and transfected into 293 cells with Lipofectamine 2000 (ThermoFisher 11668019). Adenovirus plaques appeared 7 days after transfection ...
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bioRxiv - Biochemistry 2021Quote: ... P2 or P3 baculovirus was produced in Sf9 cells and used to transfect HEK293S GnTI- suspension cells cultured in Freestyle 293 (Gibco) with 2% FBS at 37°C with 8% CO2 ...
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bioRxiv - Biochemistry 2020Quote: All ectodomains were produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m. ...
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bioRxiv - Microbiology 2021Quote: ... whilst the growth medium of T-REx-293- derived cell lines stably transfected with pcDNA4/TO-based plasmids was further supplemented with zeocin (100 µg/mL, Gibco).
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bioRxiv - Biochemistry 2020Quote: HEK293F cells were seeded in 24 well plates (1 million cells in each well) in a final volume of 1 mL of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 ug DNA as described before and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... these cells were adapted to suspension culture by growing the cells in a mix of FreeStyle(tm) 293 Expression medium (Gibco) and DMEM/FCS and decreasing the concentration of DMEM/FCS at each passage ...
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bioRxiv - Molecular Biology 2021Quote: HEK293 cells that express mNRK in a dox-dependent manner were developed using Flp-In T-REx 293 cells (Invitrogen), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... n372361, n372362) or Negative Controls (4390843, 4390846) and transfected into 293 cell lines using Lipofectamine RNAiMAX Transfection Reagent (13778030, ThermoFisher). Transfections were performed in a 24-well plate with 5 pmol of siRNA and 1.5 μl RNAiMAX Transfection reagent per well ...
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bioRxiv - Cell Biology 2021Quote: ... HEK293T cells stably expressing FLAG/HA-tagged human PRPF4 was generated using the pcDNA5/FRT-PRPF4 plasmid and Flp-In-293 cells according to the manufacturer’s protocol (Thermo Fisher). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Biochemistry 2022Quote: ... with a C-terminal Strep tag for purification and a foldon tag for trimerization was expressed in FreeStyle 293-F cells (Invitrogen). The plasmid was transiently transfected at a density of 2 × 106 cell per ml using polyethyleneimine (PEI ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: HEK293F cells were seeded in 24-well plates (1 million cells in each well) in a final volume of 1 ml of FreeStyleTM 293 medium (GIBCO). The cells were transfected with 1 µg DNA of different hSARM1 constructs and incubated at 37°C and in 8% CO2 ...
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bioRxiv - Bioengineering 2022Quote: ... mAbs were produced by transient transfection into Expi293F™ cells with equal amounts of paired heavy- and light-chain expression vectors using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (Thermo fisher). Five days post antibody expression ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Biochemistry 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for two days at which point the supernatant was harvested ...
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bioRxiv - Biochemistry 2020Quote: A 100 ml culture (1 × 106 cells/ml) of 293F cells were transfected with 100 µg of pCMV-FLAG-His6-NPC1L1-MLD plasmid using 293-fectin (Invitrogen) according to the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: NiV prefusion glycoprotein and avi-tagged probes were expressed by transfection in 293 Freestyle (293F) cells (Thermo Fisher Scientific, MA) using Turbo293 transfection reagent (SPEED BioSystem ...
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bioRxiv - Immunology 2020Quote: ... YP_009724390.1) ectodomain was produced in 500mL cultures of HEK293F cells grown in suspension using FreeStyle 293 expression medium (Life technologies) at 37°C in a humidified 8% CO2 incubator rotating at 130 r.p.m ...
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bioRxiv - Physiology 2020Quote: ... stable inducible cell lines expressing WT or mutant EAAT2 were generated by selection of Flp-In T-Rex 293 cells (Invitrogen) transfected with pcDNA5/FRT/TO-EAAT2 and used either with or without induction with tetracycline (38,50,52).
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bioRxiv - Microbiology 2021Quote: ... Transfections were carried out using 1 mg of total DNA (500 μg each of VH and Vκ plasmid DNA) and 1 l of cultured HEK293-F cell suspension maintained in sterile Freestyle 293 expression medium (Invitrogen) without antibiotics at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 3 million cells per mL were transfected using pCMV::SARS-CoV-2_S_NTD derivative mutants with the ExpiFectamine™ 293 Transfection Kit (ThermoFisher Scientific) with and cultivated for five days at which point the supernatant was harvested ...
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bioRxiv - Immunology 2019Quote: ... 1 μg/mL puromycin and expanded after adapting cells in suspension to Expi-293 medium (Life Technologies, Grand Island, NY) supplement with 1 μg/mL puromycin ...
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bioRxiv - Immunology 2020Quote: ... were transfected with the plasmid expressing S-2P protein ectodomains by ExpiFectamine 293 transfection kit and ExpiFectamine CHO transfection kit (ThermoFisher), respectively ...
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bioRxiv - Immunology 2021Quote: ... Cells grown to a density of 2.5 million cells per mL were transfected using the ExpiFectamine 293 Transfection Kit (ThermoFisher Scientific) and cultivated for four days at which point the supernatant was harvested ...
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bioRxiv - Microbiology 2020Quote: ... and HEK293S GnTI-/- cells (ATCC CRL-3022) in Freestyle 293 Expression Medium supplemented with 2% ultra-low IgG FBS (Gibco). We maintained ...
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bioRxiv - Biochemistry 2022Quote: ... RBD of SARS-CoV2 and SARS-CoV S were transiently expressed in FreeStyle™ 293-F cells (Thermo Fisher Scientific). Cells were resuspended at 1×106 per mL with fresh media on the day of transfection ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmids encoding the heavy chain and light chain of antibodies were transfected into Expi293F cells using an Expifectamine 293 transfection kit (Gibco) in a 2:1 mass ratio following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable and HEK293S GnTI-cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... sequence confirmed plasmid DNA was diluted in OptiMEM I serum-free medium and incubated for 20 minutes with ExpiFectamine 293 Reagent (Gibco). The DNA– ExpiFectamine 293 Reagent complexes were used to transfect Expi293F cell cultures in 96-deep-well blocks ...
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bioRxiv - Immunology 2022Quote: All SARS-CoV-2 S ectodomains were produced in 500-mL cultures of FreeStyle™ 293-F cells (ThermoFisher Scientific) grown in suspension using FreeStyle 293 expression medium (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Plasmids encoding both the heavy chain and light chain for each antibody were co-transfected into FreeStyle 293-F cells (Invitrogen) using polyethylenimine ...
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bioRxiv - Neuroscience 2024Quote: ... Flp-In T-REx 293 cells were grown in DMEM supplemented with 10% (v/v) fetal bovine serum (FBS; Invitrogen), 100µg/mL zeocin (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Adeno-X 293 cells (Takarabio Cat #632271) were transfected with linearized adenoviral vectors using Lipofectamine and Plus Reagent (Thermo Fisher). Cells were dislodged with gentle agitation upon exhibiting late cytopathic effect and were lysed with three freeze-thaw cycles ...
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bioRxiv - Microbiology 2024Quote: Plasmids encoding the heavy chain and light chain of the antibody were transfected at an equimolar ratio into Expi293F cells using ExpiFectamine 293 transfection kit (Gibco) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... 293 cells (106 cells) expressing CD177 were treated by 0.2 unit PI-PLC (cat# P-6466, ThermoFisher Scientific, Waltham, Massachusetts) in 0.5 ml PBS for 1 h at 37°C ...