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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: HEK 293T (ATCC® CRL-3216™) cells were maintained in standard growth medium (Gibco DMEM #11995-073) supplemented with 10% FBS (R&D Systems ...
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bioRxiv - Neuroscience 2024Quote: ... we transfected the DNA of one BC plasmid into each of the HEK samples using lipofectamine 2000 (Invitrogen) according to the manual ...
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bioRxiv - Biochemistry 2024Quote: HEK-293T cells were obtained from ATCC and grown in DMEM complete medium (Gibco, Cat# 11-965-092) supplemented with 10% fetal bovine serum (Atlanta Biologicals) ...
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bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
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bioRxiv - Microbiology 2022Quote: Recombinant proteins were produced in transiently transfected suspension culture of FreeStyle 293 cells (Thermo Fisher Scientific, Waltham, MA, USA), as previously described 66 ...
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bioRxiv - Molecular Biology 2020Quote: HEK293 cells (Flp-In 293 T-REx cell lines) were obtained from Life Technologies (Invitrogen catalogue number R780-07). Cell cultures were maintained in Dulbecco’s modified Eagle’s medium (DMEM ...
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bioRxiv - Molecular Biology 2021Quote: ... Total UPF1 depletion and RNA-seq was conducted using parental Flp-In™ T-REx™-293 cells (Invitrogen) maintained according to manufacturer instructions.
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bioRxiv - Genomics 2022Quote: ... The constructs were integrated into a specific genomic location of Flp-In T-REx 293 cells (Cat# R78007, Invitrogen) and stable cell lines were selected according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... 293 cells were transfected overnight with pEF5-FRT-TagRFP-T-IFT88 and pEZYflag-SNPH plasmids using Lipofectamine 3000 (ThermoFisher). The following day ...
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bioRxiv - Molecular Biology 2020Quote: ... Expi293F cells were transfected with pcDNA3.4 POLGARF or pcDNA3.4 (40 ug of DNA per 108 of cells) with Expifectamine 293 (Thermo Fisher). After 48hr cells were washed with PBS and lysed in a sonication bath in 1x Passive Lysis Buffer (Promega ...
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bioRxiv - Immunology 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Developmental Biology 2022Quote: ... HEK293E or HEK293-6E cells were maintained in suspension cultures in Freestyle 293 Expression Media (Gibco, Waltham, MA, USA) supplemented with heat-inactivated fetal calf serum (1% ...
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bioRxiv - Biochemistry 2019Quote: ... were maintained in suspension in protein-free chemically-defined FreeStyle 293 Expression Medium (Invitrogen / Thermo Fisher Scientific, Cat# R79007) supplemented with 1x Penicillin/ Streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Overnight culture medium was removed and cells were then serum starved in 40 μl of Freestyle 293 media (Invitrogen) per well for 4 hours at 37°C in 5% CO2 ...
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bioRxiv - Biophysics 2021Quote: ... P2 viruses (8%) were used to infect tsA201 cells grown in Freestyle 293 Expression Medium in suspension culture (ThermoFisher). Infected cells were incubated for an initial 12 h at 37 °C before 10 mM sodium butyrate was added ...
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bioRxiv - Biochemistry 2019Quote: ... HEK293F cells were cultured in serum-free Freestyle 293 Expression media using 125 mL disposable culture flasks (Thermo Fisher). Cells were shaken constantly at 125 rpm at 37°C with 5% CO2 ...
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bioRxiv - Biochemistry 2021Quote: ... The two plasmids were transiently co-transfected into HEK293F cells in FreeStyle™ 293 Expression Medium (ThermoFisher Scientific, UK). The cells were cultured in suspension phase at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting vector was transiently transfected into HEK293 cells using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific # A14525). After enhancement ...
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bioRxiv - Cancer Biology 2020Quote: ... human embryonic kidney cell line 293 and 293FT were cultured in Dulbecco’s modified Eagle’s Medium (DMEM) supplemented with 10% FBS (Life Technologies), penicillin/streptomycin (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... by transfecting 200×106 of these cells with purified DNA using the ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Supernatants from transfected cells were harvested 3 days post transfection by centrifugation at 300g for 10 min and filtration through a 22um filter ...
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bioRxiv - Genomics 2021Quote: HEK-293 cells (ATCC CRL-1573) were grown at 37°C in 5% CO2 in Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 10% FBS (Omega Scientific ...
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bioRxiv - Immunology 2020Quote: ... This plasmid was used to transiently transfect freestyle 293-F cells using the FreeStyle MAX reagent (Thermo Fisher Scientific). The spike ectodomain was purified from filtered supernatant on Streptactin XT resin (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2022Quote: ... cells were grown in suspension to 1×106 cells/mL with serum-free FreeStyle 293 expression medium (Thermo Fisher), supplemented with 25 µg/ml penicillin/streptomycin ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney line 293 (HEK293) wildtype and HTT-null cells were grown in Dulbecco’s modified Eagle’s medium (Thermofisher #41965) supplemented with 10% heat-inactivated fetal bovine serum (ThermoFisher #10500064) ...
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bioRxiv - Microbiology 2022Quote: recombinant biotinylated sE-cvD protein was expressed at 28°C using an ExpiFectamine 293 transfection kit (Thermo Fisher Scientific). Cell supernatant was harvested and dialyzed ...
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bioRxiv - Immunology 2024Quote: FreeStyle™ Expi293F™ cells were cultured in FreeStyle™ 293 expression medium according to the manufacturer’s instructions (Invitrogen). Additional cell lines were obtained from the American Type Culture Collection (ATCC) ...
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bioRxiv - Biochemistry 2022Quote: ... Glycoproteins were produced by transient transfection of exponentially growing Freestyle 293-F suspension cells (Thermo Fisher Scientific, Waltham, MA) using polyethylenimine (PEI ...
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bioRxiv - Immunology 2023Quote: ... four expression vectors were transiently transfected into Expi293 cells using ExpiFectamine 293 transfection kit according to the manufacturer’s protocol (ThermoFisher). Antibody containing cell culture supernatants were harvested after 5 days of cultivation in shake flasks ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: pcDNA4TO-TS-SNAP-β2AR or pcDNA4TO-TS-SNAP-β2AR-nLuc were stably transfected into T-RexTM-293 cells (Invitrogen) using polyethylenimine (PEI) ...
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bioRxiv - Biophysics 2023Quote: ... HEK293S GnTI- cells (ATCC CRL-3022, lot number 62430067) were cultured at 37 °C in Freestyle 293 medium (Gibco) supplemented with 2% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... Flp-In T-REx 293 cell lines with reconstituted PKR were maintained in 5% FBS DMEM supplemented with 50µg/mL hygromycin B (Invitrogen) and 15µg/mL blasticidin ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All ACE2-Fc constructs were produced in Expi293F cells (Thermo Fisher A14527) in Gibco Expi293 Expression Medium at 37°C in a humidified 8% CO2 incubator rotating at 130 rpm ...
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bioRxiv - Genomics 2022Quote: ... and staining was performed in the presence of Fc block (eBioscience/ThermoFisher, True-Stain Monocyte Blocker (Biolegend) ...
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bioRxiv - Neuroscience 2021Quote: ... cells were blocked with Fc block (CD16/32, Invitrogen, 14-0161-82) for 20 minutes on ice ...
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bioRxiv - Molecular Biology 2019Quote: ... and aliquots were incubated with Fc Block (ThermoFisher Scientific 14-9161-71) 20 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific). Medium was exchanged after 3 hours and cells were stimulated.
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bioRxiv - Genomics 2020Quote: ... washed twice in FC buffer and resuspended in Sytox Blue (Thermo Scientific) containing FC buffer for live/dead discrimination according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... supplemented with 1% penicillin-streptomycin and 10% fetal calf serum (FCS, Gibco) in an incubator (37°C ...
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bioRxiv - Neuroscience 2021Quote: ... Monocytes were immersed in 45% fetal calf serum (FCS, 10500 ThermoFisher Scientific), and 10% dimethyl sulfoxide (DMSO ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, Thermo Fisher Scientific) and 1% (v/v ...
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bioRxiv - Cancer Biology 2020Quote: ... LAIR-1 Fc was biotinylated with EZ-Link NHS-PEG4-Biotin (ThermoFisher), No-Weight Format (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... FCS and L-Glutamine were all purchased from Gibco (Thermo Fisher Scientific). The following chemicals were obtained from Roth ...
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bioRxiv - Biophysics 2020Quote: ... supplemented with 10% FCS (Eurobio) and 1% Non-Essential Amino Acids (Gibco). Primary astrocytes were obtained from E17 rat embryos following (Etienne-Manneville ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% fetal calf serum (FCS; ThermoFisher, 10499044 [Lot Number 08G8293K]) at 37°C with 5% CO2 ...
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SIMON, an automated machine learning system reveals immune signatures of influenza vaccine responsesbioRxiv - Immunology 2019Quote: ... filtered through 0.1um spin filter with 20uL/sample of Fc block (ThermoFisher) for 30min at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... in MLNL medium (low-glucose DMEM/1% FCS, Thermo Fisher Scientific 21885025). At day 2 ...
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bioRxiv - Physiology 2019Quote: ... On day 0 media was additionally supplemented with 10% FCS (Gibco#10270106). The hPCLS were treated with 10ng/ml recombinant human TGFß 1 protein (R&DSystems#240-B-010 ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 10% v/v (FCS) and 2 mM L-glutamine (Gibco). Where indicated ...
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bioRxiv - Immunology 2020Quote: ... and optical densities were acquired with a Multiskan™FC (Thermo Scientific).
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bioRxiv - Immunology 2020Quote: ... after which staining was inhibited by adding 10% FCS (10270-106, Gibco). Stained cells were washed in RPMI/PS prior to counting and addition to macrophages ...