Labshake search
Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
-
bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
-
bioRxiv - Developmental Biology 2024Quote: Primary antibodies used were HRP-conjugated anti-His (Invitrogen 46-1009; 1:2000) and mouse anti-FLAG (Sigma F1804 ...
-
bioRxiv - Molecular Biology 2024Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
-
bioRxiv - Cell Biology 2024Quote: ... His-tagged proteins were subsequently purified using Ni-NTA–agarose beads (Thermo Scientific) and elution buffer (20 mM HEPES ...
-
bioRxiv - Biophysics 2024Quote: ... and 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC, ThermoFisher). For BS3 crosslinking ...
-
bioRxiv - Immunology 2021Quote: Ab heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max (1 mg/mL ...
-
bioRxiv - Microbiology 2019Quote: ... bone marrow-derived macrophages (BMM) Vero and HEK 293T cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco) supplemented with 10% foetal calf serum (FCS ...
-
Vasohibin1, a new IRES trans-acting factor for sequential induction of angiogenic factors in hypoxiabioRxiv - Cell Biology 2019Quote: HEK-293FT cells and HT1080 cells were cultured in DMEM-GlutaMAX + Pyruvate (Life Technologies SAS, Saint-Aubin, France), supplemented with 10% fetal bovine serum (FBS) ...
-
bioRxiv - Cell Biology 2021Quote: ... Both COS-7 and HEK 293T were transfected by means of Lipofectamine and PLUS reagent (Thermo Fisher Scientific). The calcium phosphate transfection method was instead used to transfect WT or ΔOLF Lphn2-HA-pCCL in HEK 293T cells and to produce the corresponding viral particles subsequently employed to transduce ECs ...
-
bioRxiv - Microbiology 2021Quote: ... and human embryonic kidney (HEK) 293T cells (ATCC CRL-3216) were cultured in flasks containing Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco) supplemented with 10% (v/v ...
-
bioRxiv - Microbiology 2022Quote: HEK-293T cells (purchased at ATCC, CRL-3216) were grown in complete Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Microbiology 2022Quote: ... eight pHW2000 plasmids encoding all viral segments were transfected into HEK 293T cells using Lipofectamine 2000 (Thermo Scientific). Plaque assays of viral rescue supernatants were performed on MDCK-II cells to generate single plaques used for further stock generation on MDCK-II cells ...
-
bioRxiv - Immunology 2022Quote: ... or its truncation mutant were single- or co-transfected into HEK 293T cells by Lipofectamine LTX (ThermoFisher, 15338100) for 16 h ...
-
bioRxiv - Biochemistry 2020Quote: U2AF2 levels were reduced by transfection of HEK 293T cells with Stealth™ siRNA HSS117616 (Thermo Fisher Sci.) and compared to a “lo GC” negative control Stealth™ siRNA using JetPrimeR Polyplus-transfection as instructed by the manufacturer ...
-
bioRxiv - Microbiology 2022Quote: HEK-293T and HK-1 cells were maintained in Roswell Park Memorial Institute medium (RPMI 1640; Life Technologies) supplemented with 5% FCS (Sigma) ...
-
bioRxiv - Cancer Biology 2019Quote: ... HEK 293T/17 (CRL-11268) were obtained from ATCC and cultured in DMEM (high glucose, pyruvate; Gibco, 11995065), supplemented with 10% fetal bovine serum (Gemini bio-products ...
-
bioRxiv - Synthetic Biology 2020Quote: All HEK 293FT cells were grown in DMEM supplemented with 10% FBS and 1% Antibiotic-Antimycotic (Thermo Fisher) in an incubator at 37 °C and 5% CO2 atmosphere ...
-
bioRxiv - Synthetic Biology 2020Quote: All HEK 293FT cells were grown in DMEM supplemented with 10% FBS and 1% Antibiotic-Antimycotic (Thermo Fisher) in an incubator at 37 °C and 5% CO2 atmosphere ...
-
bioRxiv - Neuroscience 2019Quote: HEK 293T cells were maintained in DMEM containing 10 % fetal bovine serum (HyClone) and 1 % L-glutamine (Invitrogen). Primary hippocampal or cortical neurons were prepared from E18 Sprague-Dawley rats (Orient Bio ...
-
bioRxiv - Biochemistry 2020Quote: ... pastoris or HEK-293T cells was used to coat flat-bottom 96-well plates (Thermo Scientific NUNC-MaxiSorp) at a final concentration of 1 µg/ml (100 µl/well ...
-
bioRxiv - Biochemistry 2020Quote: ... pastoris or HEK-293T cells was used to coat flat-bottom 96-well plates (Thermo Scientific NUNC-MaxiSorp) at a final concentration of 1 µg/ml (100 µl/well ...
-
bioRxiv - Cancer Biology 2021Quote: ... pLP2 and pLP/VSVG in HEK-293T cells (ViraPower Lentiviral Packaging Mix, Thermo Fisher Scientific, Waltham, MA, USA). After virus production and subsequent titration ...
-
bioRxiv - Neuroscience 2020Quote: Transfected HEK cells seeded in 96-well plates were washed twice and incubated in FLEX buffer (HBSS (Thermofisher): 20 mM HEPES pH 7.4 ...
-
bioRxiv - Immunology 2021Quote: Ab heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max 40K (1mg/mL ...
-
bioRxiv - Immunology 2021Quote: ... B.1.351 or P.1 Spike protein were produced using HEK 293T cells transfected with Lipofectamine 3000 (ThermoFisher) using IgE-SARS-CoV-2 S plasmid variants (Genscript ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... Inducible HEK T-REx cells were cultured in Dulbecco’s modified Eagle medium GlutaMAX (Thermo Fisher Scientific, Paisley, UK) containing 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2022Quote: Lentiviral particles (LVs) were produced in HEK 293T cells cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific), supplemented with 10% FBS (EuroBio ...
-
bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles (LVs) were produced in HEK 293T cells cultured in Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific), supplemented with 10% Fetal Bovine Serum (EuroBio ...
-
bioRxiv - Immunology 2022Quote: ... HEK 293T cells (ATCC) and TZM-bl cells (NIH AIDS Reagent Program) were maintained in DMEM medium (Gibco) supplemented with 10% heat-inactivated FBS and 1% P/S ...
-
bioRxiv - Genomics 2022Quote: ... HEK-293T cells (ATCC CRL-3216) were maintained at 37°C and 5% CO2 in DMEM (ThermoFisher 11995073) with 10% FBS (Corning 35015CV) ...
-
bioRxiv - Molecular Biology 2022Quote: ... HEK-293T-CC-ORFs were cultured as above with 0.3 µg/ml of puromycin (Gibco, Cat No. A1113803) in their culture medium.
-
bioRxiv - Pathology 2022Quote: ... Calu-3 (ATCC HTB-55) and HEK 293T cells (ATCC CRL-3216) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco), with 10% fetal bovine serum (FBS ...
-
bioRxiv - Biophysics 2022Quote: HIV pseudoviruses were produced as described before by transfection of HEK 293T cells (ATCC) with Lipofectamine 2000 (Invitrogen) and the following amounts of plasmids per 10 cm dish ...
-
bioRxiv - Systems Biology 2024Quote: ... HEK-293T cells were transfected with pcDNA CVB3 or pcDNA CVB3 A67G using Lipofectamine 3000 (Thermo Fisher, L3000001). After 72 hours ...
-
bioRxiv - Immunology 2024Quote: ... R78C and R58C were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific). Proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific) ...
-
bioRxiv - Genomics 2024Quote: ... HEK 293T cells were cultured under the same conditions in Dulbecco’s modified Eagle’s medium (DMEM; Gibco #11965-092) supplemented with 5% FBS and 1% penicillin-streptomycin ...
-
bioRxiv - Molecular Biology 2024Quote: ... the HEK cells were trypsinized and cell viability was quantity using an automated cell counter (Countess from Invitrogen) at a 1:1 ratio of cells to trypan blue.
-
bioRxiv - Cancer Biology 2024Quote: ... Murine astrocytes and HEK-293FT cells were maintained in D10 medium (Dulbecco’s modified Eagle’s medium (Gibco, 11965-092), 10% fetal bovine serum ...
-
bioRxiv - Physiology 2023Quote: ... All HEK 293T cells were transiently transfected with indicated constructions using the lipo2000 (1 mg/ml, LIFE TECHNOLOGIES) reagent ...
-
bioRxiv - Microbiology 2022Quote: ... Human embryonic kidney cells (293THEK cell) (HEK 293T-ATCC-CRL-1575TM) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, GIBCO) supplemented with 10% fetal bovin serum (FBS ...
-
bioRxiv - Immunology 2023Quote: ... Antibodies were expressed through co-transfection of heavy and light chain plasmids in Expi293 HEK cells (Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2023Quote: A549 (ATCC CCL-185) and HEK-293T cells (ATCC CRL-3216) were grown in complete Dulbecco’s modified Eagle’s medium (DMEM, Gibco) supplemented with 10% (v:v ...
-
bioRxiv - Genomics 2022Quote: HEK-293T cells were cultured in 15-cm2 dishes in Dulbecco’s Modified Eagle’s Medium (DMEM) (Gibco #11995-065) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2023Quote: Human embryonic kidney (HEK) epithelial-like cell line HEK293T was maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) (11965-118; GIBCO™ ...
-
bioRxiv - Immunology 2023Quote: Antibody heavy and light plasmids were co-transfected at a 1:1 ratio into HEK-293F cells (Thermofisher) using PEI Max (1 mg/mL ...
-
bioRxiv - Immunology 2023Quote: ... Clonal genes were delivered as purified plasmid DNA ready for transient transfection in HEK Expi293 cells (Thermo Scientific). Cultures in a volume of 1.2 ml were grown for four days ...
-
bioRxiv - Genetics 2023Quote: ... Human embryonic kidney (HEK) 293T cells (CRL-11268, ATCC) line was cultured in Dulbecco’s Modified Eagle’s Medium (11965-092, Gibco) supplemented with 10% FBS (26140079 ...
-
bioRxiv - Bioengineering 2023Quote: HEK-293T cells (ATCC CRL-3216) were cultured in D10 medium: {DMEM high-glucose medium (Gibco, cat. #11965118) supplemented with 10% fetal bovine serum (Omega Scientific ...
-
bioRxiv - Immunology 2023Quote: ... All constructs were transiently co-transfected into HEK Expi293F cells using ExpiFectamine to produce SIgA complex (Thermo Fisher). Six days after transfection ...