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Citations for Thermo Fisher :
2801 - 2850 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and gently dissociated by trituration in Hibernate E medium (Life Technologies, cat#A1247601). Neurons were diluted in 5 ml Neuron Growth Medium (Neurobasal media (Life Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... and products separated on 2% EX e-gels containing SYBR Gold II (Invitrogen). qRT-PCR using custom TaqMan primers/probes was performed with TaqMan Fast Advanced Master Mix on a QuantStudio 3 Real-Time PCR System ...
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bioRxiv - Microbiology 2023Quote: ... After two washes with Gibco William’s E Medium (Thermo Fisher Scientific, Cat# A1217601), cells were observed under the EVOS M7000 Imaging System (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Four percent agarose EX E-gels were purchased from Invitrogen (cat. no. G401004). All other chemicals were purchased from Sigma-Aldrich.
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bioRxiv - Immunology 2023Quote: ... The PCR product sizes were verified on agarose gel (E-Gel EX; Invitrogen) and quantified with fluorometry (Qubit ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were size-selected with E-Gel EX 2% agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit (QIAGEN) ...
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bioRxiv - Microbiology 2023Quote: ... MHC II (I-A/I-E) (SuperBright645, Thermo Fisher, 1:200,M5/114.15.2), MHC I (H-2Kb/Db ...
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bioRxiv - Bioengineering 2023Quote: ... and 33% complete cholangiocyte culture medium (CCCM: William’s E medium (Gibco, Life Technologies) supplemented with 10 mM nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2023Quote: ... and 33% complete cholangiocyte culture medium (CCCM: William’s E medium (Gibco, Life Technologies) supplemented with 10 mM nicotinamide (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: ... DNA quality was checked on a 1% agarose gel (E-gel EX Invitrogen). 500ng DNA was used from each sample for further analysis ...
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bioRxiv - Physiology 2023Quote: ... H&E sections images were taken using EVOS M7000 imager (Thermo Fisher Scientific) using 20X magnification and analyzed using ImageJ (http://imagej.nih.gov/ij/) ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
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bioRxiv - Neuroscience 2024Quote: ... Embryonic neuronal tissue was dissected and stored in hibernate E medium (ThermoFisher A1247601) on ice ...
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bioRxiv - Genetics 2021Quote: ... Epitope-tagged bait proteins were detected with mouse anti-c-myc monoclonal antibody (Invitrogen, MA1-980) and IR-680 conjugated donkey anti-mouse secondary antibody (LI-COR ...
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bioRxiv - Evolutionary Biology 2021Quote: ... T0M20 antibodies (polyclonal, cat number PA5-52843) and Alexa 568-tagged secondary antibodies were from ThermoFisher. The imaging was performed using Nikon SP-1 microscope and analyzed using FIJI (Schindelin et al. ...
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bioRxiv - Cell Biology 2019Quote: Creation of the 3xmEGFP tagged RabE12 construct occurred by a two-element LR Gateway reaction (ThermoFisher) of entry clones pENT-L1-3xmEGFP-L5r and pENT-L5-RabE12-L2 ...
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bioRxiv - Neuroscience 2020Quote: ... N-terminally HA-tagged AMPAR constructs were expressed from the doxycycline inducible pcDNA4/TO vector (Invitrogen Cat ...
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bioRxiv - Biochemistry 2021Quote: Immunoprecipitation of HA tagged GAPDH (TDH3-HA) was performed using Pierce anti-HA beads (Thermo Fisher). Briefly ...
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bioRxiv - Biochemistry 2021Quote: ... 200 μl and the tagged Aβ(1-40) is incubated with Ulp1 SUMO-protease (either Invitrogen or produced recombinantly (Malakhov ...
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bioRxiv - Biophysics 2021Quote: ... they were transfected with EGFP tagged wt LA/A350P DNA along with Lipofectamine 2000 (Invitrogen, USA) in a ratio 1:1.5 ...
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bioRxiv - Bioengineering 2022Quote: MCF-7 Cells were fluorescently tagged with CellTracker™ Green CMTPX dye (Invitrogen™, Waltham, MA). A stock solution of 10 mM was prepared according to manufacturer’s instructions ...
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bioRxiv - Genetics 2022Quote: 3T3-L1 cells expressing GFP-tagged human CIDEC were incubated with MitoTracker (#M7512; Thermo Fisher Scientific) before fixation ...
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bioRxiv - Neuroscience 2020Quote: ... In some cases 200-400 nl of fluorescent-tagged material (20% solution of FluoroEmerald Molecular Probes, Eugene ...
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bioRxiv - Microbiology 2020Quote: The Avi-tagged BG505 gp140.664.R1 trimer probe was transiently expressed in HEK293F cells (Thermo Fisher) (67) ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein A-tagged proteins were detected with HRP-conjugated polyclonal anti-Protein A (Invitrogen, PA1-26853) at 1:5000.
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bioRxiv - Immunology 2020Quote: ... The resultant FLAG-tagged STAT3 constructs were transfected into 293T using lipofectamine 3000 (Thermo Fisher Scientific). 48 hours after transfection cells were lysed (50mM Tris ...
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bioRxiv - Genetics 2019Quote: ... Proteins tagged with the V5 epitope (Hxk2, Reg1) were detected with the Anti-V5 probe (Invitrogen) diluted 1:1,000 ...
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bioRxiv - Plant Biology 2022Quote: ... GST and GST-tagged SAMS were incubated with 40 μl GST magnetic beads (Thermo Fisher Scientific) for 1 h at 4°C and then these beads were washed three times with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... Tagged gDNA was enriched using Dynabeads™ MyOne™ Streptavidin C1 (Thermo Fisher Scientific, Cat. 65001). The beads were allowed to room temperature while being resuspended on a HulaMixer™ Sample Mixer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... the cells were probed with donkey anti-mouse secondary antibody tagged with Alexa Fluor 568 (Invitrogen), for 1 h in dark ...
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bioRxiv - Microbiology 2023Quote: ... we used PrepEase Histidine-tagged Protein Purification Midi Kit-High Yield (Affymetrix, Santa Clara, CA, USA) or Protino Ni-IDA 2000 packed columns (Macherey-Nagel ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Genetics 2023Quote: ... Flag-tagged proteins were immunoprecipitated with anti-Flag magnetic agarose (Pierce Anti-DYKDDDDK Magnetic Agarose, ThermoFisher), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... and stained with Alexa Fluor 555-tagged donkey-α-goat secondary antibody (Molecular Probes, 1:1000) for 1 hour ...
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bioRxiv - Biophysics 2023Quote: ... Hexa-histidine tagged mTHSD7A fragments were purified under native conditions using Ni-NTA resin (Thermo Scientific) applying the batch method according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Rab7a and LAMP1 wt and mutant molecules tagged with EGFP using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged HCT-116 cells were seeded in FluoroBrite DMEM (Thermo Fisher, cat. no. 1896701) supplemented with 10% FBS (Corning) ...
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Molecular Biology 2024Quote: ... Tissues from Knock-in LAP-tagged PAF1C flies were detected with anti-V5 tag (Invitrogen, R96025) 1:500 in BBX incubated overnight at 4°C with nutation ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the fiber was thermally desorbed in the gas chromatographer (GC/MS) injection port followed by separation of the volatiles under programmed conditions using an ISQ LT and Trace 1310 (Thermo Fisher Scientific GmbH, Dreieich, Germany) device equipped with a ZB5 column (30 m ...
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bioRxiv - Biophysics 2021Quote: ... the protein construct was transformed into the Escherichia coli BL21 (DE3 Invitrogen) competent cells ...
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bioRxiv - Genetics 2021Quote: ... coli and extracted using the GeneJET Plasmid Miniprep Kit (Thermo Scientific™). The construction was verified using enzymatic digestion with KpnI and PvuI.
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bioRxiv - Microbiology 2019Quote: ... coli BL21 cells were stained with VybrantTM DiO live cell tracer (ThermoFisher). Cell-labeling solution (5 μl ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli strains were grown in Lucia Bertani (LB) medium (Fisher Scientific) with aeration at 37°C ...
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bioRxiv - Evolutionary Biology 2021Quote: ... coli cultures using PureLink HiPure Plasmid Midiprep Kit (Invitrogen, Cat. No. K210005). To facilitate DNA delivery to ESCs through nucleofection ...
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bioRxiv - Genomics 2020Quote: ... coli (One-Shot MAX Efficiency DH5α-T1 Competent Cells, ThermoFisher, CA# 12297016), followed by plating on LB+Carb plates ...
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bioRxiv - Neuroscience 2019Quote: ... coli BioParticles™ Conjugate (Thermo Fisher Scientific, #P35361, 66.7 ng per well). iMGLs or primary human microglia were incubated with conjugated-synaptosomes (3.44 mg/ml) ...
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bioRxiv - Genetics 2020Quote: ... Escherichia coli strain DH5α was used according to the manufacturer’s recommendations (Invitrogen). Bacterial transformants were selected on LB agar ...
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bioRxiv - Immunology 2019Quote: ... coli TOP10F cells via TOPO cloning (Thermo Fisher Scientific, Waltham, MA, USA). After digesting the purified plasmids containing each individual cloned gene with restriction enzymes ...
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bioRxiv - Microbiology 2019Quote: ... Escherichia coli strains were cultivated in LB medium (Thermo Fisher Scientific, USA) supplemented with appropriate antibiotics ...