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Citations for Thermo Fisher :
2751 - 2800 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were digested and ligated into pcDNA3.1 myc-His B expression vectors (Invitrogen), with a 3xFlag epitope tag also fused to the N-terminus of Ptchd1 ...
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bioRxiv - Biochemistry 2022Quote: ... redundant anti-His antibody was washed away and 15 μL chemiluminescence substrate (Thermo Scientific) was added onto each well to generate chemiluminescence signal ...
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bioRxiv - Cancer Biology 2022Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI FBS, Gibco™ Cat. #10438026) and 1% antibiotic/antimycotic (Gibco™) ...
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bioRxiv - Plant Biology 2022Quote: ... and purified by Dynabeads™ His-Tag Isolation and Pulldown (ThermoFisher, Catalog number 10103D). DA1 swapped protein ubiquitylation reactions used E1 ...
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bioRxiv - Molecular Biology 2023Quote: ... incubated for 30 minutes on ice with His-Pur Ni-NTA resin (Thermo Scientific), packed to a height of approximately 5 cm in polypropylene columns ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was then processed through a His-tag cobalt resin (ThermoFisher, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We then used the Dynabeads His-Tag Isolation and Pull- down kit (ThermoFisher 10103D) to isolate his-tagged Fcy1 following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... and His-p115 were affinity purified using Ni-NTA HisPur beads (Thermo Fisher Scientific) from crude E ...
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bioRxiv - Microbiology 2023Quote: ... A standard curve of 10-fold dilutions of plasmid pAc5.1-V5-His-A (Invitrogen) containing the DCV ORF-2 sequence was used to convert Ct values to genome copy numbers.
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bioRxiv - Evolutionary Biology 2022Quote: ... and probed with 6x-His Tag Monoclonal Antibody (HIS.H8) (Thermo Fisher Scientific #MA1-21315) diluted in 5% milk in PBS-T (1:500) ...
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bioRxiv - Molecular Biology 2022Quote: ... Junction PCR was performed with Extensor Hi-Fidelity PCR Master Mix (Thermo Fisher Scientific) in a 20 μL reaction using 500 nM of target-specific primers and the following cycling conditions ...
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bioRxiv - Neuroscience 2023Quote: ... and subcloned into the pcDNA™3.1/myc-His(−)A vector (Thermo Fisher Scientific). We accidentally obtained the pcDNA™3.1/myc-His(−)A-(G4C2)9 vector at this step ...
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bioRxiv - Immunology 2023Quote: ... M4 cells are maintained in DMEM/F12 media with 5% HI HS (Gibco #26050088).
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bioRxiv - Bioengineering 2023Quote: ... were first coated with 100 ng of mouse anti-His antibody (Invitrogen, MA1-21315) and blocked ...
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bioRxiv - Immunology 2024Quote: ... HI samples were incubated with 1 mL of IgG Fc Capture Select (Thermo Fisher) resin at 4C for 20 to 48 h to bind IgG ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then heated with Novex Hi-Density TBE sample buffer (Invitrogen LC6678) at 95°C for 5 min ...
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bioRxiv - Bioengineering 2024Quote: ... supplemented with 10% heat inactivated fetal bovine serum (HI-FBS) (10082147, Thermo Fisher Scientific) and 1% penicillin/streptomycin 1000 U/mL (Pen/Strep ...
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bioRxiv - Developmental Biology 2024Quote: ... wild type Cic and mutant variants were subcloned into pMT-V5-His vector (Invitrogen).
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bioRxiv - Cell Biology 2020Quote: ... The HepaRG cells (Biopredic, France) were cultured in William’s E medium (Life Technologies) supplemented with 10% of FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-MHCII (I-A/I-E) (ThermoFisher Scientific/eBioscience 14-5321-82).rat anti-ICAM2 (BD Biosciences 553326) ...
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bioRxiv - Molecular Biology 2019Quote: LMH/2A cells (ATCC CRL-2118) were grown in Williams’ E medium (GIBCO) without phenol red ...
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bioRxiv - Immunology 2021Quote: ... Libraries were size selected with E-Gel EX 2% agarose gels (Life Technologies) and purified by QIAquick Gel Extraction Kit (Qiagen) ...
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bioRxiv - Plant Biology 2020Quote: ... and size-selected with the E-Gel size select (Life Technologies, CA, USA). Approximately 350 bp of library fragments were retrieved ...
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bioRxiv - Bioengineering 2020Quote: ... Nested PCR products were evaluated on 2% 96-well E Gel (Life Technologies), and positive wells with a specific band of ∼450 bp were purified and sequenced ...
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bioRxiv - Bioengineering 2021Quote: DNA sizes were first confirmed by running 1-2% Agarose E-Gel (Invitrogen). Then ...
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bioRxiv - Systems Biology 2020Quote: ... PHHs were cultivated in Williams medium E (Biochrom) supplemented with 10% FCS (Gibco), 100 nM dexamethasone ...
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bioRxiv - Microbiology 2021Quote: ... Product sizes were confirmed using the e-Gel 2% agarose (GP) (Invitrogen, USA) followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher ...
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bioRxiv - Bioengineering 2020Quote: ... 1b and below and maintained in William’s E Medium (WEM) containing Glutamax (Gibco), ITS+ (Corning) ...
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bioRxiv - Microbiology 2020Quote: ... BF-2 and E-11 cells in 24 well cell culture plates (Gibco) and incubated at 25°C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: The EnzChek® Lysozyme Assay (Thermo Fisher Scientific, Waltham, MA, Cat. E-22013) was used according to manufacturer’s protocols to determine lysozyme activity in serum of a subset of American alligator serum samples (Lake Waccamaw ...
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bioRxiv - Genomics 2022Quote: ... Gel purification of pooled products from a 2% E-gel EX (Life Technologies) were performed using the QiaQuick Gel Extraction kit (Qiagen).
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bioRxiv - Developmental Biology 2022Quote: ... E-cadherin (Cell Signaling 3195, 1:200 or Invitrogen 13-1900, 1:200), Lef1 (Cell Signaling 2230 ...
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bioRxiv - Microbiology 2021Quote: ... 200 ng of DNA was run on a 4% EX E-gel (ThermoFisher). DNA with a size of at least ~200 bases was excised and purified using a gel DNA recovery kit (Zymo Research ...
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bioRxiv - Developmental Biology 2020Quote: ... Quality of amplified product was checked with a 2% E-Gel (ThermoFisher Scientific) before Illumina sequencing adapters were added in a subsequent PCR reaction ...
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bioRxiv - Pathology 2019Quote: ... and visualized on 1% agarose e-gel Power Snap (ThermoFisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: ... The PCR products were analyzed on an E-Gel 1% agarose (Thermofisher Scientific). The sequences of Luc/GFP primer set used to amplify the sg mRNA were ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by size-selection and extraction from a 2% E-gel EX (Invitrogen). Single-end 84 nt sequence reads were generated using the NextSeq 500 system (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... and rat anti-E-cadherin (1:1000, Thermo Fisher, Cat. No. 13-1900). Sections were washed in PBS with 0.1% Tween-20 four times for 5 minutes at room temperature and incubated in secondary antibodies and DAPI (1:500 dilution of 1 mg/ml stock ...
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bioRxiv - Immunology 2019Quote: ... Round 2 PCR amplicons were visualized using precast 1% agarose E-gels (Invitrogen), purified with the AMPure XP magnetic bead purification system (Agencourt) ...
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bioRxiv - Immunology 2021Quote: ... human immunodeficiency virus backbones expressing firefly luciferase (pNL43R-E-luciferase) and pcDNA3.1 (Invitrogen) expression vectors encoding the SARS-VoV-2 S protein were co-transfected into 293T cells (ATCC) ...
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bioRxiv - Biophysics 2020Quote: ... soluble E-cadherin ectodomains were selected with 200 µg/mL Hygromycin B (Invitrogen). Western blots of the culture medium confirmed protein expression by individual colonies ...
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bioRxiv - Cell Biology 2022Quote: ... the EnzChek® Phosphate Assay Kit (#E-6646, Thermo Fisher Scientific, Waltham, MA) was used ...
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bioRxiv - Microbiology 2022Quote: ... or SARS-CoV-2 E protein (1 to 75 amino acids) (ThermoFisher Scientific) in coating buffer [0.05 M sodium carbonate-sodium bicarbonate (pH 9.6)] at 4°C for overnight incubation ...
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bioRxiv - Molecular Biology 2022Quote: ... sections prepared with a Microm HM 335 E microtome (Thermo Scientific,Walldorf, Germany) and mounted on Superfrost Plus slides (Thermo Scientific) ...
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bioRxiv - Physiology 2022Quote: ... cells were resuspended in William’s E medium (Thermo Fisher Scientific, Cat No. 12551032) with 5% FBS (Gibco ...
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bioRxiv - Genetics 2024Quote: ... the medium was exchanged for maintenance medium (William’s E Medium (WEM, GIBCO A1217601) supplemented with GIBCO #CM4000).
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bioRxiv - Molecular Biology 2024Quote: ... E-PAP treated RNA was purified using PureLink micro RNA purification kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The amplified DNA was resolved on E-Gel 2% agarose gel (Invitrogen, G401002) on E-Gel Power Snap Electrophoresis device (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: Cortices were dissected from E18 mice and kept in Hibernate-E (Gibco #A1247601) at 4C until dissociation within 1 to 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... J-Lat A1 (gift from E. Verdin) were cultivated in media RPMI (Thermofisher) supplemented as described for the DMEM medium and ...