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Citations for Thermo Fisher :
2851 - 2900 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... coli One Shot BL21 (DE3) chemically competent cells (Thermo Fisher, Ref: C600003) were transformed with the pACYC184 plasmid according to standard protocol and the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting proteins were expressed in Escherichia coli BL21(DE3) cells (Invitrogen) after induction by 0.5 mM isopropyl β-D-1-thiogalactopyranoside at 18°C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The construct was expressed in Escherichia coli strain BL21(DE3) star (Invitrogen) as a fusion protein with a His tag ...
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bioRxiv - Biochemistry 2021Quote: ... coli BL21 Codon-Plus (DE3-pLys) chemically competent cells (Invitrogen, Carlsbad, CA). Transformed cells with kanamycin and chloramphenicol resistance were cultured at 37 °C with shaking (180 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... coli 70S•PR20 —were collected on a Talos electron microscope (ThermoFisher Scientific) operating at 200 KV and equipped with a K3 direct electron detector (Gatan Inc. ...
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bioRxiv - Plant Biology 2021Quote: ... and introduced into Escherichia coli Mach1 T1R competent cells (Thermo Fisher Scientific). Colonies possessing the fragment of the mutated NbPDS3 gene were identified by colony PCR using primers M13 forward ...
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bioRxiv - Biophysics 2020Quote: ORF1 expression plasmids were transformed into Escherichia coli BL21STAR(DE3) cells (Invitrogen). One-liter cultures supplemented with 50 μg/mL kanamycin were inoculated with 20 mL of an over-night culture and grown at 37°C to an OD600 between 0.5 and 0.8 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli DH5α cells using a GeneJET Plasmid Miniprep kit (ThermoFisher Scientific K0503). Plasmids were generated by standard restriction digests ...
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bioRxiv - Biochemistry 2022Quote: ... The plasmids were transformed into Escherichia coli BL21(DE3)pLysS cells (Invitrogen), and cells harboring the plasmids were grown at 37°C until the OD600 reached 0.7-1.0 in Luria Bertani (LB ...
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bioRxiv - Microbiology 2022Quote: ... coli by the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA) and modified or used for full-length genome assembly.
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bioRxiv - Microbiology 2022Quote: ... coli using the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher, Waltham, MA). Table S3 lists the primers used for the construction of the variant S DNA fragments.
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bioRxiv - Immunology 2019Quote: ... Ifit2 and Ifit3 were expressed in BL21 (DE3) Star Escherichia coli (Invitrogen). Cells were grown to an OD600 of ~1.0 in 2× TY media at 37 °C ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli DH5α or MegaX-DH10B™ T1R Electrocomp™ cells (ThermoFisher Scientific). Transformants were cultured en masse on Luria-Bertani (LB ...
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bioRxiv - Molecular Biology 2019Quote: ... GST fusion proteins were expressed in Escherichia coli BL21 Star (DE3) (Invitrogen) or C41 (86 ...
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bioRxiv - Microbiology 2020Quote: ... coli were grown aerobically at 37°C in Luria-Bertani broth (Affymetrix) supplemented with ampicillin (50 ug/mL) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... coli (DH5α) with an endotoxin-free plasmid extraction kit (Invitrogen, Carlsbad, CA) and transfected into ExpiCHO-S cells using ExpiFectamine Transfection Kit (Gibco) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... the protein was expressed in Escherichia coli BL21(DE3) cells (Life Technologies) using a pET-46 tobacco etch virus (TEV ...
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bioRxiv - Immunology 2020Quote: ... or live Escherichia coli cells were supplemented with fluorescently labeled dextran (Invitrogen, 10,000 MW at 1:100 dilution of a 5 ng/nL stock ...
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bioRxiv - Genetics 2020Quote: ... Plasmids were transformed into Stbl3 chemically competent Escherichia coli (Thermo Fisher Scientific) and colonies containing correct plasmids were selected by colony PCR followed by Sanger sequencing (at Fasteris ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 Star™ (DE3) One Shot® cells (Thermo Fisher Scientific) were used for protein expression (Dennehy et al. ...
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bioRxiv - Biochemistry 2021Quote: ... The Escherichia coli TOP 10 competent cells were purchased from Life Technologies. The genes were synthesized by IDT (Coralville ...
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bioRxiv - Microbiology 2021Quote: ... coli isolates were grown on Brilliance GBS Agar/Oxoid (Thermo Fisher Scientific) and in Luria-Bertani broth ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Biochemistry 2022Quote: ... coli One Shot ccdB Survival 2 TIR competent cells (Invitrogen, ThermoFisher Scientific) and selected on LB agar supplemented with 50 µg/mL kanamycin ...
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bioRxiv - Neuroscience 2022Quote: ... Chemically competent Stbl3 Escherichia coli cells (Thermo Fisher Scientific, Waltham, MA, USA) were transformed with the AAV plasmids ...
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bioRxiv - Cell Biology 2022Quote: All DNA constructs were produced using Escherichia coli DH5a (Thermo Fisher Scientific) and extracted using a plasmid midiprep kit from Qiagen ...
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bioRxiv - Immunology 2024Quote: ... fluorescein-conjugated Escherichia coli K-12 BioParticles (MOI 5, 15 min, Invitrogen), or fluorescein-conjugated S ...
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bioRxiv - Biochemistry 2024Quote: ... coli One Shot™ ccdB Survival™ 2 T1R Competent Cells (ThermoFisher). Single colonies were cultured for plasmid isolation and the cloned sequence was confirmed by DNA sequencing.
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bioRxiv - Biochemistry 2024Quote: ... coli (DH5α) cells were seeded into 50mL of 2YT + Amp100 (Fisher Scientific) medium and grown overnight at 37°C ...
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bioRxiv - Bioengineering 2024Quote: ... The expression plasmid was then transformed into Escherichia coli BL21lDE3 Star (Thermofisher) for use in protein production ...
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bioRxiv - Biochemistry 2023Quote: ... Competent bacteria (Escherichia coli, strain TOP10, Thermo Fisher Scientific, cat. no. C404010) were transformed with the resulting recombination products ...
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bioRxiv - Cancer Biology 2023Quote: ... GST-ISG15 was expressed in Escherichia coli BL21(DE3) (Thermo Fisher, EC0114) at OD600 0.6-0.8 with 0.1 mM IPTG and 20°C and lysed in the lysis buffer by sonication ...
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bioRxiv - Microbiology 2023Quote: ... coli strains grown overnight on Lennox Broth (LB) (Bacto Tryptone (#211699, Gibco) 10g/L ...
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bioRxiv - Cell Biology 2023Quote: ... coli strain OP50 containing 500 nM MitoTracker Deep Red FM (Invitrogen M22426) until day 1 adults ...
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bioRxiv - Microbiology 2022Quote: ... coli One Shot® ccdB SurvivalTM 2 T1R competent cells (Life Technologies), which was grown in LB agar plates (10 g l-1 NaCl ...
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bioRxiv - Microbiology 2022Quote: ... coli and Chocolate agar with Vitox (Oxoid™, Thermo Fisher, Darmstadt, Germany) for N ...
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bioRxiv - Microbiology 2023Quote: ... coli BL21 (DE3) RIL cells following the manufacturer’s protocol (Invitrogen, Karlsruhe, DE). Each protein was then isolated and affinity-purified using an amylose resin according to the manufactureŕs protocol (New England Biolabs ...
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bioRxiv - Plant Biology 2023Quote: ... coli strain BL21 and purified using PierceTM glutathione magnetic agarose (ThermoFisher Scientific). The purified protein was digested with PreScission Protease (APEXBIO ...
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bioRxiv - Immunology 2023Quote: ... coli One Shot™ TOP10 electrocompetent cells (Thermo Fisher Scientific, Waltham, MA) in a 0.1 cm cuvette using a BTX ECM 630 electroporator (BTX ...
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bioRxiv - Cell Biology 2023Quote: ... coli RNA or DNA by adding 1 µL RNase Cocktail (ThermoFisher Scientific) and 1U TurboDNase (ThermoFisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... coli using chemically competent cells (Subcloning EfficiencyTM DH5α Competent Cells, ThermoFisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... coli BL21 (DE3) RIL cells following the manufacturer’s protocol (Invitrogen, Karlsruhe, Germany). The protein was then purified by affinity-purification using an amylose beads according to the manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Genomics 2023Quote: ... Alexa 488 conjugated Escherichia coli (K-12 strain) BioParticles (cat: E13231, ThermoFisher) was added at 10ug/well ...
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bioRxiv - Biochemistry 2023Quote: ... coli cells and libraries into MegaX DH10B T1R Electrocomp Cells (Thermo Fisher), before plated on LB plates containing either carbenicillin (100 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... coli cultures were routinely cultured in Lysogenic Miller broth/agar (Fisher Scientific). For all other purposes ...
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bioRxiv - Molecular Biology 2024Quote: ... The ligation reaction was transformed into DH5α Escherichia coli (ThermoFisher Scientific, USA) grown on LB plates with 100 μM Ampicilin (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... coli was purchased from the GeneArt synthesis service (Thermo Fisher Scientific, USA). The recombinant plasmids were transformed into E ...
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bioRxiv - Plant Biology 2024Quote: ... Ligation products were electroporated into Escherichia coli DH10B Electromax electrocompetent cells (Invitrogen). Transformed cell suspensions were plated on Luria-Bertani (LB ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant enzymes were probed using anti-V5-HRP conjugated antibody (Invitrogen), which was detected using an ECL Advance Western Blotting Detection Kit (Amersham ...
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bioRxiv - Immunology 2021Quote: ... and recombinant proteins were purified using Ni-NTA agarose (Thermo Fisher Scientific), then buffer-exchanged into PBS and concentrated using Amicon Ultra centrifugal filters (MilliporeSigma) ...