Labshake search
Citations for Thermo Fisher :
3051 - 3100 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... Species-specific secondary antibodies tagged with Alexa Fluor corresponding to 568 nm emission spectra (1:1,000, Thermofisher) was used for immunofluorescence ...
-
bioRxiv - Microbiology 2022Quote: ... HNRNPUL1 and MECR were recombined into V5-tagged mammalian expression constructs by Gateway cloning into pcDNA6.2 (Invitrogen) and pLX304 (Addgene 25890) ...
-
bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-rabbit or anti-human IgG (H+L) (Life Technologies) respectively ...
-
bioRxiv - Molecular Biology 2021Quote: ... Flag tagged proteins were immunoprecipitated from 2-3mg cellular extracts with Pierce Anti-DYKDDDDK Magnetic Agarose (Invitrogen). Beads were washed 5x with 1ml IP lysis buffer end eluted with 50µl 1xLaemmli buffer ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μg of Flag-Strep-Strep-(FSS)-tagged LRRK2RCKW cDNA and Lipofectamine 2000 reagent (ThermoFisher Scientific, USA) were used according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2020Quote: ... while non-Avi tagged antigens were biotinylated chemically using EZ-Link Sulfo-NHS-Biotin (Thermo Fisher, A39256). VH and VL sequences of SARS-CoV-2 control antibodies (COV2-2196 ...
-
bioRxiv - Cell Biology 2021Quote: ... Cross-absorbed secondary antibodies tagged with Alexa-fluorophores were diluted in blocking buffer (1:400, Invitrogen, Switzerland) and applied for 30 min at RT ...
-
bioRxiv - Molecular Biology 2024Quote: ... They were then transfected with Halo-tagged gene constructs using Lipofectamine LTX (Thermo Fisher Scientific, MA, USA). After 48 hours ...
-
bioRxiv - Molecular Biology 2024Quote: ... HEK293 and MS2-tagged HEK293:24xMS2-NEAT1 cells were cultured in Dulbecco’s modified eagle’s medium (DMEM, Gibco) with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2024Quote: HEK cells and zebrafish samples were tagged with TMT10plex™ Isobaric Label Reagent Set (Thermo Scientific, #90111), and fibroblast cells with TMTpro™16plex Isobaric Labels (Thermo Scientific ...
-
bioRxiv - Immunology 2024Quote: ... FLAG-tagged constructs were PCR amplified with Gateway adapters and cloned into pDONR221 using BP Clonase (Invitrogen). Hoil1 and Hoip mutations and deletions were generated using the Q5 site-directed mutagenesis kit (New England Biolabs) ...
-
bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged proteins were concentrated using 30K MWCO concentrators to ∼ 5 mg/ml (Thermo Scientific™, USA). Protein was stored at −80°C.
-
bioRxiv - Developmental Biology 2024Quote: ... inserts were then subcloned into pCS2+ N HA tagged vectors that had been converted into Gateway (Invitrogen) destination vectors ...
-
bioRxiv - Immunology 2023Quote: ... Hexahistidine-tagged proteins were then detected using mouse anti-6xHis tag antibody (Ma1-135, Invitrogen, Karlsruhe, Germany) as primary antibody and signals revealed by horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (ab6789 ...
-
bioRxiv - Biophysics 2023Quote: ... they were transfected with the S6-tagged human TrkA by means of Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer instructions ...
-
bioRxiv - Molecular Biology 2023Quote: ... Protein A-tagged strains were immunoprecipitated with Rabbit IgG-conjugated (MP-Biomedicals, SKU 085594) Dynabeads (Invitrogen, 14301) (Vakiloroayaei et al. ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were incubated with Alexa Fluor 555 tagged donkey-α-rabbit secondary antibody (1:1000; Molecular Probes) and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000 ...
-
bioRxiv - Cell Biology 2023Quote: ... followed by incubation with fluorescence-tagged secondary antibodies (Molecular Probes Alexa series, all 1:400 in PBS) for 1h at room temperature ...
-
bioRxiv - Molecular Biology 2023Quote: ... IPs of FLAG-tagged PRRC2B and PRRC2B fragments were performed using anti-DYKDDDDK Magnetic agarose (ThermoFisher Scientific). Beads were mixed with 70 μl of SDS loading buffer (Bio-Rad ...
-
bioRxiv - Developmental Biology 2023Quote: ... Biotin-tagged DNA nicks were visualized using Alexa488-or Alexa647-conjugated streptavidin (Molecular Probes, diluted 1/1000) during the incubation with the secondary antibody.
-
bioRxiv - Immunology 2023Quote: ... The supernatant fraction containing the GST-tagged constructs were loaded onto Protino Glutathione Agarose 4B (Fisher Scientific) beads and impurities were removed by washing with TBS ...
-
bioRxiv - Cell Biology 2023Quote: MDA-MB-231 clones H2 and C10 carrying endogenously tagged HiBiT-cIAP1 were generated by Thermo Fisher and were used for compound screens ...
-
bioRxiv - Neuroscience 2023Quote: ... Slides were next incubated with AlexaFluor-555 tagged donkey-α-rabbit secondary antibody (1:1000, Molecular Probes) for one hour and imaged with a Nikon Eclipse 90i or a Nikon Eclipse Ti2 inverted microscope ...
-
bioRxiv - Biochemistry 2023Quote: ... FLAG-tagged ARL15 proteins were immunoprecipitated using 50 μL of Pierce Anti-DYKDDDDK Magnetic Agarose (ThermoFisher Scientific) for 2 hours at 4 ºC ...
-
bioRxiv - Neuroscience 2023Quote: ... VF-tagged human SYK and FCER1G plasmids for mammalian expression were generated using Gateway cloning technology (Invitrogen) as described previously (47) ...
-
bioRxiv - Neuroscience 2023Quote: ... The nucleotide sequence encoding the histone H2B-tagged GFP was designed and ordered via GeneArts (ThermoFisher Scientific). Subsequently ...
-
bioRxiv - Molecular Biology 2023Quote: ... His8-tagged TGM2 was first pulled down by BSA-blocked Ni2+-NTA agarose beads (Thermo Fisher Scientific). Next ...
-
bioRxiv - Cancer Biology 2024Quote: MOLT-4 cells expressing HiBiT-tagged CDK9 were cultured in RPMI 1640 medium (Thermo Fisher Scientific, 11875093) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Synthetic Biology 2021Quote: ... The purified pooled product was loaded onto 2% EX Agarose E-Gel (Thermo Fisher Scientific Inc. ...
-
bioRxiv - Immunology 2021Quote: ... Plat-E cells in logarithmic growth phase were seeded in dishes (Nunc™ 150350). The next day ...
-
bioRxiv - Immunology 2019Quote: ... Correct PCR product sizes were verified on an agarose gel (E-Gel EX; Invitrogen) and quantified with fluorometry (Qubit ...
-
bioRxiv - Bioengineering 2020Quote: ... and analyzed by gel electrophoresis using 1% (E-Gel EX, #G401001, Thermo Fisher Scientific) and 2% agarose gels (E-Gel ...
-
bioRxiv - Bioengineering 2020Quote: ... 5 μm-thick serial sections were stained with hematoxylin and eosin (H&E; ThermoFisher) or Von Kossa (VK ...
-
bioRxiv - Genetics 2019Quote: ... The resulting cDNAs were separated on a 4% agarose E-Gel (Invitrogen, Life Technologies), and the 140–160 nt length species were selected ...
-
bioRxiv - Genetics 2019Quote: ... The resulting cDNAs were separated on a 4% agarose E-Gel (Invitrogen, Life Technologies), and the 140–160 nt length species were selected ...
-
bioRxiv - Bioengineering 2021Quote: ... 5 μm-thick serial sections were stained with hematoxylin and eosin (H&E; ThermoFisher) or Von Kossa (VK ...
-
bioRxiv - Neuroscience 2021Quote: ... cells were incubated with A647-complexed EGF (1:300; Molecular Probes, Cat# E-35351) for 15 min ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tissue sections were immunostained with primary antibodies: E-cadherin (1:100; Invitrogen, ECCD-2), cytokeratin-8 (1:100 ...
-
bioRxiv - Cancer Biology 2020Quote: ... which was visualized on an E-Gel Imager Blue-Light Base (Thermo Fisher Scientific). The presence of the successfully amplified ampr PCR product was confirmed using a standard 500 bp ladder.
-
bioRxiv - Cancer Biology 2020Quote: ... and 5 μl of purified products were loaded on 2% E-Gel EX (Invitrogen) to verify the reaction performance and amplicon size ...
-
bioRxiv - Cell Biology 2021Quote: For electron microscopy experiments dissociated neuronal pellets were resuspended in Hibernate-E medium (Gibco) supplemented with B27 (Invitrogen ...
-
bioRxiv - Microbiology 2020Quote: ... and E (France and Switzerland) were immediately added to RNAlater® (ThermoFisher Scientific, UK) and stored at −20°C ...
-
bioRxiv - Microbiology 2020Quote: PCR products were visualized on 2% agarose E-gels (Invitrogen, Carlsbad, CA, United States). PCR products were then purified using the Nucleospin Gel and PCR kit (QIAGEN ...
-
bioRxiv - Molecular Biology 2020Quote: ... with a E-Gel 1-Kb Plus DNA Ladder (Life Technologies, #10488090, Carlsbad, CA) as a reference ...
-
bioRxiv - Cancer Biology 2021Quote: ... Inducible E-cad vector was generated by annealing the ORF-CDH1 (Clone ID:IOH46767, Invitrogen) cassette into an inducible backbone (pLIX403 ...
-
bioRxiv - Cell Biology 2021Quote: ... and resuspended in William’s E Medium (WEM) with GlutaMAX™ (32551-087, Invitrogen, USA) containing 10% FBS (17L624 ...
-
bioRxiv - Microbiology 2022Quote: ... The PCR product was purified using E-GelTM SizeSelectTM II 2% agarose gel (Invitrogen). RNA-seq library for 300bp insert size was constructed following the manufacturer’s instructions (NEBNext UltraTM II DNA Library Prep Kit) ...
-
bioRxiv - Cell Biology 2020Quote: ... were purchased from Thermo Fisher Scientific and cultured in Williams’ Medium E (1x, no phenol red) (Thermo Fisher Scientific) following the manufacturer’s instructions.
-
bioRxiv - Microbiology 2022Quote: ... with Sarbeco E gene primers and probe in a QuantStudio 3 thermocycler (Applied Biosystems). Percent inhibition of virus infectivity was determined by comparing viral load in treated wells with the viral load in untreated control wells ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... and separated using 0.8% agarose gel electrophoresis with E-Gel CloneWell (Thermo Fisher Scientific). The cDNA samples were cloned into a pUC19 vector (PCR-amplified with primers 11 and 12 ...