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Citations for Thermo Fisher :
2601 - 2650 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... Verification of a successful GST-tagged AP2 protein purification by GST-affinity purification (Thermo Scientific Pierce Glutathione Superflow Agarose beads ...
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bioRxiv - Molecular Biology 2023Quote: ... The Fc-tagged ACE2 or mAb were first immobilized on Protein G Dynabeads (ThermoFisher Scientific). The S protein solutions were then incubated with on-bead receptor or antibody at room temperature for 60 min ...
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was isolated from clonal CRISPR tagged cells using Genomic DNA Mini Kit (Invitrogen) according to manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary hybridization was performed using fluorescently tagged antibodies: AF647 goat anti-rabbit (A2l24S, Invitrogen, l:l000), AFS94 goat anti-rabbit (All037 ...
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bioRxiv - Biophysics 2021Quote: ... coli NA22 mixed with Nile Red (Thermo scientific, 200 μg/ml). These plates were incubated at 25°C for dauer formation ...
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bioRxiv - Microbiology 2020Quote: ... purified Escherichia coli RNA using RNeasy columns (Invitrogen, Carlsbad, CA, USA) was also tested ...
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bioRxiv - Biochemistry 2020Quote: ... One Shot® TOP10 Chemically Competent Escherichia coli (Thermo Fisher Scientific) cells were transformed to amplify the mutated genes ...
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bioRxiv - Biochemistry 2020Quote: ... were transformed into Escherichia coli production strain Rosetta (DE3) cells (Invitrogen). Production of the maltogenic α-amylase LaGH13_20 and the oligosaccharide 1,4-α-glucosyltransferase LaGH13_31B was carried out using a 5 L bioreactor (Biostat B ...
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bioRxiv - Microbiology 2020Quote: ... coli DE3 BL21AI (Life technologies, Van Allen Way Carlsbad, CA, USA). All plasmids and primers used in this study are listed in Supplementary table 2 and Supplementary table 3 respectively.
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bioRxiv - Microbiology 2021Quote: ... coli cells using PureLink HiPure Plasmid Maxiprep Kit (Thermo Fisher Scientific). Then OmRV-fragment1/pMK was digested with DraIII and XhoI at 37°C overnight ...
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bioRxiv - Cancer Biology 2022Quote: ... coli using the TOPO™ TA Cloning™ Kit (ThermoFisher, 451641). Plasmid DNA from bacterial colonies was extracted using Zyppy Plasmid Miniprep Kit (Zymo research ...
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bioRxiv - Microbiology 2022Quote: ... coli were seeded in sterile Difco™ Nutrient Broth (Fisher Scientific) and incubated at 37°C degree overnight prior to each experiment ...
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bioRxiv - Systems Biology 2022Quote: ... coli’s genome with high-fidelity Phusion Polymerase (Thermo Scientific, Dreieich, Germany) using primer pairs alaA-amp_fwd ...
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bioRxiv - Biochemistry 2022Quote: ... coli strain BL21-star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA), and the transformed cells were cultured in Luria–Bertani medium at 37 °C ...
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bioRxiv - Biophysics 2022Quote: ... coli BL21 Star™ (DE3) or BL21 (DE3) pLysS (Invitrogen, MA) cells in Luria Broth or M9 media supplemented with 15NH4C1 (1 g/L ...
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bioRxiv - Developmental Biology 2022Quote: ... coli Texas Red (ThermoFisher E2863, 20 mg/ml in 1X PBS), Zymosan (Sigma Aldrich Z4250 ...
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bioRxiv - Microbiology 2020Quote: Plasmids were cloned using Escherichia coli DH5α cells (Invitrogen, Karlsruhe, Germany). Construction of deletion mutants was performed by homologous recombination ...
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bioRxiv - Immunology 2022Quote: ... coli BioParticles™ conjugates (Invitrogen, Thermo Fisher cat #P35361 and #P35366) were used ...
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bioRxiv - Immunology 2022Quote: ... coli BioParticles™ conjugates (Invitrogen, Thermo Fisher cat #P35361 and #P35366) were used ...
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bioRxiv - Microbiology 2021Quote: ... coli cells depleted from rRNA (MICROBExpress Bacterial mRNA Enrichment Kit (Invitrogen)) was used as cleavage substrate ...
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bioRxiv - Microbiology 2021Quote: ... Escherichia coli BL21 (DE3) strain was purchased from Invitrogen (California, USA). Ni2+-NTA His-bind slurry was obtained from Novagen (Darmstadt ...
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bioRxiv - Biophysics 2021Quote: ... 60 Polyprotein constructs were transformed into Escherichia coli BL21 (DE3 Invitrogen) competent cells ...
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bioRxiv - Genetics 2021Quote: ... Reactions were transformed into chemically competent TOP10 Escherichia coli (Invitrogen, C404010), plated and selected on lysogeny broth (LB)- agar plates with ampicillin (100 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... coli and purified using a GeneJET plasmid miniprep kit (Thermo Fisher). To express anti-DEC205-OVA ...
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bioRxiv - Biochemistry 2022Quote: Proteins were expressed in Escherichia coli BL21(DE3) cells (Life Technologies). Full-length and ΔAF-1 PPARγ proteins were expressed using an autoinduction procedure where cells were grown for 6 hrs at 37 °C followed by addition of 0.1 mM zinc chloride ...
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bioRxiv - Genetics 2022Quote: ... coli bacteria and with 18 ul 10% 2-butanone (Acros Organics) in ethanol on the lid for 1 hr ...
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bioRxiv - Genetics 2022Quote: ... coli competent cells (18265-017) were purchased from Invitrogen (Waltham, MA).
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bioRxiv - Plant Biology 2022Quote: ... T1 phage-resistant Escherichia coli Mach-1 cells (Invitrogen, Carlsbard, USA) were used for general cloning ...
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bioRxiv - Microbiology 2022Quote: ... coli O157 latex agglutination test kit (Thermo Fisher Scientific, Waltham, MA) to confirm serotype ...
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bioRxiv - Microbiology 2022Quote: ... coli MG1655 were cloned into the pJET1.2 cloning vector (Thermo Scientific). The number of 16S rRNA gene copies in stool were then determined using quantitative PCR with the above primers for a concentration range of the pJET1.2-16S construct and the DNA isolated from 400 mg stool ...
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bioRxiv - Biochemistry 2022Quote: The bacteria used in this study were Escherichia coli DH5α (Invitrogen), E ...
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bioRxiv - Systems Biology 2022Quote: ... coli cells on top of a thin coverslip (Fisher Scientific, USA) sealed with wax to an aluminum holder ...
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bioRxiv - Microbiology 2024Quote: ... Escherichia coli strains were grown in Luria-Bertani (LB; ThermoFisher Scientific) medium at 37 °C in a MaxQ 6000 incubator (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... coli (Andrews, 2001) were determined using MIC Test strips (Fisher Scientific) and the results were presented in Supplementary Table S1 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and then transformed into TOP10 Escherichia coli competent cells (Invitrogen, USA). The transformants were grown on ampicillin-supplemented plates ...
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bioRxiv - Biochemistry 2023Quote: ... coli LOBSTR cells and purified using Ni-NTA resin (Thermo Fisher) followed by size exclusion chromatography on a Sephacryl S-100 column ...
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bioRxiv - Biochemistry 2023Quote: ... coli BL21 Star™ (DE3)pLysS strain (#C602003, Thermo Fisher Scientific) was transformed with pDest-His6-MBP-PDE6D and grown at 37 °C in LB medium supplemented with ampicillin at 1:1,000 dilution from 100 mg/ mL stock ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and transformed into TOP10 Escherichia coli competent cells (Invitrogen, CA, USA). Transformants were grown on ampicillin-supplemented plates ...
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bioRxiv - Microbiology 2023Quote: ... coli TGCR isolates were extracted using DNAzol® (Invitrogen™, USA) following manufacturer protocol and recommendations ...
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bioRxiv - Microbiology 2023Quote: ... coli DH5α or One Shot® ccdB SurvivalTM 2 T1R (Invitrogen) for plasmids containing the ccdB containing Gateway cassette were used.
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bioRxiv - Biochemistry 2023Quote: ... coli and BacuroDirect C-term Linear DNA (Thermo Fisher Scientific, #12562019) for protein expression in insect cells by the Gateway LR reaction ...
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bioRxiv - Biochemistry 2023Quote: ... coli strain was BL21(DE3) from Invitrogen (One ShotTM BL21(DE3), Cat ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the protein was expressed in Escherichia coli Rosetta (DE3)pLysS (Invitrogen) cells that had codon-optimized gene in the pETSUMO vector ...
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bioRxiv - Microbiology 2020Quote: ... with 0.1 ng/mL human recombinant epidermal growth factor (EGF; Gibco) and 0.05 mg/ml bovine pituitary extract (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... and human recombinant insulin (2.5 µg/mL, Thermo Fisher Scientific, 12585014). From Day 1 to 11 ...
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bioRxiv - Cell Biology 2020Quote: ... Ki-67 recombinant rabbit monoclonal antibody (SP6) (Thermofisher, Cat. #: MA5-14520), Goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Cell Biology 2020Quote: ... containing 10 ng/ml recombinant human IL-2 (Gibco, Thermo Fisher), 10% heat-inactivated Fetal Calf Serum (Gibco ...
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bioRxiv - Genetics 2021Quote: ... and Mouse M-CSF Recombinant Protein were from Invitrogen (Carlsbad, CA). Lipopolysaccharides from Escherichia coli O111:B4 was purchased from Sigma (St Louis ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant soluble ACE2 was coated on NUNC Maxisorp plates (Thermo Scientific) at 1µg/well at RT for 3 h ...