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Citations for Thermo Fisher :
2751 - 2800 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Goat anti-Rabbit IgG (H+L) Alexa Fluor 647 conjugated (Invitrogen, A-21245, dilution 1:500); Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Human IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11013, dilution 1:500). Data acquisition was performed with FACSVerse™ (BD ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-Rabbit IgG (H+L) Alexa Fluor 488 conjugated (Invitrogen, A-11008, dilution 1:500); Goat anti-Human IgG (H+L ...
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bioRxiv - Cell Biology 2023Quote: ... cDNA was generated from 1 μg RNA using RevertAir H Minus reverse transcription (Thermo Scientific, 11541515) with RNasin (Promega ...
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bioRxiv - Neuroscience 2023Quote: ... Donkey anti-chicken IgY (H+L) Alexa Fluor 647 (1:500, Thermo Fisher Scientific, A78952, RRID:AB_2921074).
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bioRxiv - Microbiology 2023Quote: ... Membranes were blocked for at least 1 h in TBS containing T20 Starting Block (Thermo Fisher) and incubated with antisera against reovirus (1:5000 ...
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bioRxiv - Cell Biology 2023Quote: Secondary antibodies: donkey anti-rabbit IgG (H+L) Alexa Fluor 568 (1:500 IF; Thermo Fisher Scientific Cat# A10042 ...
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bioRxiv - Cell Biology 2023Quote: ... blocked in 2.5% BSA for 1 h and mounted in Mowiol mounting Media with DAPI (Invitrogen). Images were taken at 10X for crypt quantification ...
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bioRxiv - Neuroscience 2023Quote: ... Goat anti-rabbit IgG (H+L) Alexa Fluor 568 (1:500, Thermo Fisher Scientific, A11011, RRID:AB_143157), Donkey anti-chicken IgY (H+L ...
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bioRxiv - Physiology 2023Quote: ... and goat anti-rabbit IgG H+L Alexa Fluor 488 (1:1000, Thermo Fisher Scientific, USA) were carried out at room temperature for 1 hour each ...
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bioRxiv - Neuroscience 2023Quote: ... Alexa Fluor 647 Goat anti-Rabbit IgG (H+L) (Thermo Fisher Scientific A-21245, 1:200).
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies were diluted 1:500 (goat anti-Rabbit IgG Alexa-647 H+L Invitrogen #A21245 and goat anti-Mouse IgG Alexa-405 H+L Invitrogen #A31553) ...
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bioRxiv - Plant Biology 2023Quote: ... Equal loading was confirmed by staining for >1 h in PageBlue™ protein staining solution (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: ... Samples were then incubated for 1 hour at 37°C with 4LU RNase H (Invitrogen™) or mock-treated as a control prior to reverse transcription using oligo (dT ...
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bioRxiv - Neuroscience 2023Quote: ... for 1 h at room temperature and the signals were visualized using chemiluminescence reagents (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... goat anti Guinea Pig IgG H&L A647 (1:500, Thermo Fisher Scientific, cat#: A-21450), goat anti-Mouse IgG1 A488 (1:500 ...
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bioRxiv - Immunology 2023Quote: ... for 1 h before pulsing during the last 10 min with puromycin (10 μg/mL; ThermoFisher). After 3 washes with ice-cold PBS ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were digested at 37°C for 1 h with DpnI (Thermo Scientific™, FD1704) and transformed into NZY5α competent cells (Nzytech MB00402) ...
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bioRxiv - Microbiology 2024Quote: ... fluorescently labeled secondary antibodies Goat anti-Mouse IgG (H+L) AF568 (1:400; Thermo Scientific, #A21245), Goat anti-Rabbit IgG (H+L ...
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bioRxiv - Cell Biology 2024Quote: ... 1 μg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Scientific) with gene specific primers ...
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bioRxiv - Developmental Biology 2024Quote: ... and Alexa Fluor 555 goat anti-Mouse IgG (H+L) (Thermo Fisher, A-21422; 1:500).
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Physiology 2022Quote: ... An aliquot of 100 μL was subsequently derivatized using a final concentration of 10 mM aniline and 5 mM 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) (ThermoFisher) for 2 h at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... 25 μl/well of 60 mM water solution of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC, Thermo Fisher Scientific) were added ...
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bioRxiv - Immunology 2022Quote: ... with siRNAs against SAMHD1 (sense RNA 5’-GCAGAUAAGUGAACGAGAUTT-3’, antisense RNA 5’-AUCUCGUUCACUUAUCUGCAG-3’) or the negative control #1 siRNA (Ambion). Three days after transfection with either siRNA or shRNA plasmids ...
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bioRxiv - Neuroscience 2023Quote: ... The carboxylic groups on the carbon fiber surface were electro-activated by incubation in 0.4 M 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC; Life Technologies) and 0.1 M and N-hydroxysulfosuccinimide (NHSS ...
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bioRxiv - Neuroscience 2022Quote: ... the sgRNA sequence targeting exon 1 of Faah (5’-CTGCAGGCTAGGCAAACC-3’) and a control sgRNA sequence (5’-CTGCAGGCTAGGCAAACCTTT-3’ were synthesized (Invitrogen) and cloned into the shuttle plasmid for adeno-associated viral (pAAV-FLEX-SaCas9-U6-sgRNA;Addgene #124844 ...
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bioRxiv - Developmental Biology 2021Quote: ... Secondary antibodies and stains: Goat anti-Rabbit IgG (H+L) Alexa 488 and 594 (1:1000 cells, 1:500 tissue Invitrogen; A-11008, A-11012), Goat anti-Mouse IgG1 Alexa 488 (1:500 Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were incubated for 1 h at RT with appropriate secondary antibodies conjugated to Alexa Fluor 488 or 594 (Molecular Probes, ThermoFisher 1:500) diluted in 0.1% BSA ...
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bioRxiv - Bioengineering 2020Quote: ... samples were washed three times with PBS and incubated with goat anti rabbit and goat anti mouse secondary antibodies for (Alexa Fluor 568) 1 h with (Thermo Fisher Scientific, 1:100). After washing ...
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bioRxiv - Immunology 2020Quote: ... Both the dorsal and ventral halves (with the epidermis side facing upwards) were incubated for 1 h at 37°C in 1 ml IMDM (Thermo-Fisher Scientific, Waltham, MA, USA) medium containing 1U Dispase II (Thermo-Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1%NGS+0.4%TritonX+PBS and incubated with secondary antibodies for 1 hour at room temperature with anti-mouse IgG (H+L) Alexa Fluor 555 (1:200, Thermo Fisher, Catalog# A-21422) or anti-rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... was added to the cells and kept in 37°C humidified incubator for 1 h followed by incubation with fluorescein isothiocyanate (FITC)-conjugated goat anti-mouse secondary antibody (Invitrogen, USA,1:500 dilution) and Alexa Fluor 594 conjugated goat anti-mouse (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... This was followed by a 1 h room temperature incubation with a horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA). The membranes were developed using Clarity Western ECL (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: ... The blots were then incubated for 1 h at room temperature with secondary HRP-conjugated goat anti-mouse goat antibodies (Invitrogen #31430, 1:5000 dilution). The blot was developed using SuperSignal West Femto Maximum Sensitivity ECL Substrate (Thermo Fischer Scientific 34094 ...
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bioRxiv - Cancer Biology 2023Quote: ... coverslips were washed twice with PBS and incubated for 1 h at RT with Alexa647-conjugated anti-rabbit secondary antibody (Invitrogen, #A-31573, 1/500) to reveal both p62 and PPT1 proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1x PBS and blocked for 1 hour (h) in blocking buffer made of washing buffer and 1% heat-inactivated goat serum (HIGS) (Life Technologies, Waltham, MA, USA). Skin sections were fixed in 4% PFA for 10 min before they were washed and blocked in blocking buffer containing 10% HIGS ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed three times with PBS and incubated with secondary antibodies at 1:2000 dilution for 1 h and DAPI (Thermo Fisher Scientific cat#D1306) at 1:1000 for 5 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... and incubated for 1 h with a 1:1000 dilution of Alexa Fluor 488 conjugate Goat anti-Mouse IgG (Invitrogen A32723, Rockford, Illinois, USA) and Alexa Fluor 594 conjugate Goat anti-Mouse IgG (Invitrogen A32742 ...
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bioRxiv - Bioengineering 2021Quote: ... Apoptosis was detected by incubating devices with 5μM of CellEvent™Caspase 3/7 Green detection reagent (Invitrogen) for 30 minutes at 37°C.
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bioRxiv - Immunology 2020Quote: ... splenic NP366-374 TMEM were assessed with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay kit (ThermoFisher). Lung single cells were stained with surface markers then incubated with caspase 3/7 green detection reagent for 30 minutes at 37°C as described in the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Live/Dead™ Cell Imaging Kit and CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) were used for detection of cell apoptosis based on the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... 10% H2/N2 (BOC) for 3 days on blood agar plates containing 7% laked horse blood (Thermo Scientific) and the Campylobacter selective supplement “Skirrow” containing the antibiotics trimethoprim ...
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Microbiology 2022Quote: ... Proliferating organoids were grown for 4–7 days and dissociated using TrypLE Express Enzyme (Thermo Fisher Scientific) for 10–20 minutes in a 37°C water bath ...
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bioRxiv - Developmental Biology 2021Quote: ... Cell between passage 4 to 7 were transfected with si-SLC38A5 siRNA (Cat# 4392420, Thermo Fisher Scientific) or negative control siRNA (si-Ctrl ...
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bioRxiv - Microbiology 2023Quote: ... Parasites (CDC1132 or G3 strain) were labelled with Cell Tracker Blue CMAC (7-amino-4-chloromethylcoumarin) (Invitrogen), added to confluent BPH-1 cells (1:3 parasite ...
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bioRxiv - Molecular Biology 2021Quote: ... and CAF-1 p60 (5′-AAUCUUGCUCGUCAUACCA-3′) were transfected using RNAi MAX (Invitrogen).
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Planarian CREB-binding protein (CBP) gene family regulates stem cell maintenance and differentiationbioRxiv - Developmental Biology 2020Quote: ... and TO-PRO®-3 (1:3000, Thermo Fisher Scientific, Waltham, MA, USA).