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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... pH 7.4) for 10mins and later incubated with 4 uM fluo-4/AM (1 mM, Molecular probes, #F14201) and 0.002% pluronic F-127 (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Anti-mouse SARS-CoV-2-spike (GTX, 1:100) and anti-rabbit hACE2 (Thermo Fisher,1:100) were applied overnight at 4 °C followed by incubation of appropriate secondary antibodies conjugated with fluorophores ...
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bioRxiv - Molecular Biology 2021Quote: ... at a ratio of 1:2:1 using the transfection reagent (Lipofectamine 3000, Thermo Fisher Scientific #L3000001). Six hours after the transfection ...
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bioRxiv - Developmental Biology 2020Quote: mESC lines were cultured in 2-inhibitors based medium (Advanced DMEM/F12:Neurobasal (1:1) medium (Gibco), supplemented with 2.5% ESC-grade fetal bovine serum (vol/vol ...
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bioRxiv - Genetics 2022Quote: ... samples were denatured in 1:1 with 2 x SDS buffer with 1x reducing agent (Invitrogen, NP0004) for 10 min at 95 °C ...
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bioRxiv - Immunology 2020Quote: ... gp120 in PBS (200μg/ml for 100μl/20μg/mice) was mixed at a 1:1 ratio with Alhydrogel 2% (Invitrogen) and injected intraperitoneally ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% antibiotic-antimycotic and 1% protein-free hybridoma medium II (PFHM 2) (Thermo Fisher Scientific) and the respective growth factors and small molecules (8 µM GSK-3 inhibitor CHIR99021 (R&D Systems) ...
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bioRxiv - Molecular Biology 2023Quote: ... and BE(2)-C were cultured in a 1:1 mixture of DMEM/F12 (with HEPES, Gibco) and EMEM (ATCC) ...
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bioRxiv - Biophysics 2023Quote: ... A preheated (90°C, 1 min) number 1 German borosilicate sterile 2 well chamber coverglass slide (ThermoFisher) was loaded with 750 μL of melted 0.7% Omnipur agarose (EMD Millipore) ...
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bioRxiv - Cancer Biology 2023Quote: ... her4.1:GFP) were prepared at 2:1:1 molar ratios in 50% TE Buffer (Invitrogen, 12090-015) with KCl (final concentration 0.1M ...
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bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
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bioRxiv - Microbiology 2020Quote: ... The wells were washed 3 times with 1 mL PBS 1X and covered with a mixture containing secondary goat anti-rabbit-Alexa488 antibodies (Invitrogen Molecular Probe 2mg.mL-1, 1/300e) and DAPI (1/100e ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM PMSF) and precleared for 1 hour at 4°C with 25 μl of protein G Dynabeads (Invitrogen). The precleared chromatin solution (1.5 × 106 cells ...
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bioRxiv - Developmental Biology 2019Quote: ... prior to overnight incubation at 4°C in mouse anti-ZO-1 (Life Technologies, Carlsbad, CA; 1A12; 1:100). Following further buffer washes ...
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bioRxiv - Cancer Biology 2020Quote: ... new organoids were fixed with 4% paraformaldehyde for 1 day and then incubated with the phalloidin (1:40; Invitrogen) and Hoechst 33342 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Primary staining’s were performed overnight at 4 °C with antibodies to TRF2 and POT-1 (1:300; Abcam and ThermoFisher,); CD3 (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Genomics 2024Quote: ... was coupled for 4 hours to 40µl of 1:1 mix Protein G and Protein A Dynabeads (Thermo Scientific) and used for overnight chromatin immunoprecipitation in IP buffer (1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... This was followed by overnight incubation at 4°C with 1:200 anti-LAMP-1 (D401sm #15665, Life Technologies). The next day ...
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bioRxiv - Cell Biology 2023Quote: ... The relative expression of target cDNAs within the sample set was calculated from a serial dilution (1:4–1:256) (standard curve) of a cDNA pool using StepOneTM software V2.0.2 (Applied Biosystems). Real-time PCR data were then normalized to elongation factor-1 alpha (eF1α ...
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bioRxiv - Molecular Biology 2023Quote: ... The following primary antibodies and dilutions were used overnight at 4 °C in PBST with 1% milk: mouse anti- V5 conjugated to horseradish peroxidase (HRP; 1:5,000; Invitrogen), mouse anti-V5 (1:5,000 ...
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bioRxiv - Bioengineering 2024Quote: ... Plasma samples were thawed from -80°C storage to room temperature and diluted 1:4 with 1× PBS (Gibco). Samples were then transferred to a 96-well clear bottom black plate (Thermo Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked for at least 1 h in TBS containing T20 Starting Block (Thermo Fisher) and incubated with antisera against reovirus (1:5000) ...
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bioRxiv - Cell Biology 2020Quote: ... HeLa cells were stained for 1 h with LipidTOX Deep Red dye (Thermo Fisher Scientific, H34477). Subsequently ...
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bioRxiv - Cell Biology 2020Quote: ... 1 µg of total RNA was reverse transcribed using Maxima H Minus Reverse Transcriptase (Thermo Fisher) and 5 µM random hexamer primers ...
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bioRxiv - Developmental Biology 2020Quote: ... Secondary antibodies were Alexa Fluor 555 goat anti rabbit IgG (H+L) (1:1000; #A27039; Invitrogen) and Alexa Fluor 488 goat anti chicken IgY (H+L ...
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bioRxiv - Neuroscience 2021Quote: ... Protein bands were revealed with 1 h staining with SimplyBlue Safe stain (Thermo Fisher Scientific, LC6065) and overnight de-staining in water ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 μg RNA was reverse transcribed to cDNA with Maxima H minus Reverse Transcriptase (Thermo Scientific) using the following amplification program ...
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bioRxiv - Genomics 2020Quote: ... incubated with secondary antibodies for 1 h and detected using ECL or Femto substrates (Thermo Scientific) and LAS ImageQuant System (GE Healthcare ...
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bioRxiv - Bioengineering 2019Quote: ... they were incubated for 1 h at 37 °C with Alexa Fluor 532 Phalloidin (Invitrogen, USA) to stain the actin cytoskeleton ...
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bioRxiv - Pathology 2019Quote: ... Goat anti-rat IgG (H+L) Alexa Fluor 680 (Molecular Probes A-21096, RRID:AB_141554, 1:500); Goat anti-Chicken IgY (H+L ...
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bioRxiv - Pathology 2019Quote: ... Goat anti-rat IgG (H+L) Alexa Fluor 568 (Molecular Probes A-11077, RRID:AB_141874, 1:500); Goat anti-rat IgG (H+L ...
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bioRxiv - Genetics 2021Quote: ... and anti-mouse IgG (H+L) Alexa Flour 594 (Thermo Fisher Scientific, A-21203; 1:2,000) secondary antibodies at room temperature for 1 h ...
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bioRxiv - Developmental Biology 2020Quote: ... and donkey anti-goat IgG (H+L) Alexa Fluor 546 (1:1000, ThermoFisher, no. A-11056). Antibody labeling was performed according to manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: ... and Alexa Fluor 488 goat anti-chicken IgG (H+L) (ThermoFisher Scientific, A-11039, 1:500).
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were HRP-conjugated goat anti-Rabbit IgG (H+L) (1:10000, ThermoFisher Scientific 31460) and HRP-conjugated goat anti-Mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... Alexa Fluor 647 donkey anti-mouse IgG (H+L) (A31571) (Molecular Probes, 1:500 (Figure 5) or 1:200 (other figures)).
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bioRxiv - Cell Biology 2021Quote: ... and goat anti-mouse Alexa Fluor 568 IgG H +L (1:400, Thermo Fisher Scientific, A11004). All immunolabeling steps were carried out at 37°C with gentle shaking ...
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bioRxiv - Systems Biology 2020Quote: ... 1.65 μL of Nuclease-free water and 1 μL of Maxima H Minus RT (Thermo Scientific). Finally ...
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PSGL-1 inhibits HIV-1 infection by restricting actin dynamics and sequestering HIV envelope proteinsbioRxiv - Microbiology 2020Quote: ... Alexa Fluor 488 goat anti-mouse IgG(H+L) (Molecular Probes®, A-11001, 1:1000), Alexa Fluor 594 goat anti-mouse IgG(H+L ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibodies were: Alexa Fluor 488 goat-anti-rabbit IgG (H+L) (Invitrogen, A11008, 1:1000); Alexa Fluor 488 donkey-anti-mouse IgG (H+L ...
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bioRxiv - Cell Biology 2020Quote: ... and 488-conjugated goat anti-rabbit IgG (H+L) (Thermo Fisher Scientific #A11008, diluted 1:400) for 1 h ...
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bioRxiv - Biochemistry 2022Quote: ... goat anti rabbit IgG (H+L) cross absorbed Alexa Fluor 488 (Invitrogen, 11008, 1:5000 dilution) and goat anti mouse IgG (H+L ...
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bioRxiv - Cell Biology 2022Quote: ... Signal was detected incubating for 1.5 h with the corresponding Alexa secondary antibody (1:500, Invitrogen). For double immunostaining with anti-pMLKL (1:200 ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 633 Goat anti rabbit IgG(H+L) Secondary Antibody (Thermofisher, A-21070, 1:500) and DAPI (Sigma Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... Alexa Fluor 633 Goat anti rabbit IgG(H+L) Secondary Antibody (Thermofisher, A-21070, 1:500), and DAPI (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... was added for 1 h followed by Alexa Fluor 488-labeled goat anti-mouse IgG (Invitrogen) for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... transfected Hek293T Hrd1 knockout cells were starved for 1 h in methionine/cysteine free DMEM (Invitrogen) supplemented with 10% dialysed fetal calf serum ...