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Citations for Thermo Fisher :
3001 - 3050 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... Cell surface staining was done in live cells blocked with diluted superblock (1:4 dilution in PBS with 1 mM MgCl2 and 0.1 mM CaCl2, ThermoFisher Scientific) at 4°C for 0.5 h ...
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bioRxiv - Cancer Biology 2024Quote: ... the Live/Dead assay was conducted using 1 μM calcein AM and 4 μM ethidium homodimer-1 (Life Technologies kit) for 30 minutes at 37 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... mono-Methylated K35 overnight (1:100) at 4°C followed by 1:100 Alexa-Fluor 488 and Alexa-Fluor 555 (both Invitrogen) for 2 h at RT.
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bioRxiv - Cell Biology 2023Quote: ... 1.2 M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 µg/mL DAPI (4’, 6-diamidino2-phenylindole; Molecular Probes). Cells were imaged with a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Microbiology 2023Quote: ... and incubated for 45 minutes with 1:500 AlexaFluor 488-conjugated anti-mouse (Invitrogen, 1:500 in 4% BSA PBS) plus DAPI (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... and then incubated for 2 hours at room temperature with secondary antibodies in PBS (Donkey anti-Mouse 647 nm 1:1000, Abcam ab15107, lot GR3234832-4; Donkey anti-Rabbit 594nm 1:1000, Invitrogen A21207 ...
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bioRxiv - Bioengineering 2023Quote: ... 105 rested NY-ESO-1 TCR+ T cells (4:1 CD8+:CD4+) were labeled with 0.5uM CSFE (Thermo Fisher Scientific) and co-cultured with 105 NucLight red+ tumor cells in complete medium ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated overnight at 4 °C with the following antibody: ERα (Dilution 1:1 µg/mL; Invitrogen, CAT: MA5-13191). IRDye secondary antibody (Dilution 1:20,000 ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275; Phosphorylated-cofilin 1:500 Invitrogen #44-1072G and β-actin 1:3000 ...
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bioRxiv - Immunology 2023Quote: ... The upper aqueous layer was removed to a new tube and mixed 1:1 with Isopropanol (Fisher Scientific, BP2618-4) and incubated for 1 min with glycoblue co-precipitant (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Cell pellets were harvested by centrifugation at 4°C and resuspended in 40 mL 1:1 PBS:Bacterial Protein Extraction Reagent (BPER, ThermoFisher Scientific). Cell pellets were lysed by incubating in 60°C water bath for 30 minutes ...
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bioRxiv - Microbiology 2024Quote: ... Two skin swab samples were taken in a 5 mL centrifuge tube and mixed with 1 mL of pre-cooled methanol-water (V:V=4:1, Thermo Fisher), after brief sonication in ice water for 20 min ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the membranes were incubated 1 h at room temperature with the peroxidase-labelled polyclonal secondary anti-rabbit antibody (#31430; 1:10000, Thermo Fisher Scientific, Waltham, MA, USA). After washing ...
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bioRxiv - Cancer Biology 2022Quote: ... The sections were incubated with Alexa Fluor 488 donkey anti-rabbit IgG and Alexa Fluor 633 goat anti-mouse IgG secondary antibodies for 1 h (1:200; Molecular Probes by Life Technologies, Waltham, MA, USA). Control slides were incubated with the secondary antibody alone ...
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bioRxiv - Physiology 2020Quote: ... Slides were washed twice with PBS and then incubated for 1 h at room temperature with Alexa Fluor® 488-labeled goat anti-rabbit IgG (1:500; Molecular Probes, Inc., Eugene, OR) diluted in PBS with 2% BSA ...
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bioRxiv - Physiology 2019Quote: ... sections were incubated for 1 h at room temperature with anti-rabbit Alexa Fluor A488 antibody (A21206, 1:1000, Thermo Fisher Scientific, Waltham, MA, USA). Slides were washed with PBS and coverslipped with DAPI Fluoromount-G (0100-20 ...
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bioRxiv - Cell Biology 2021Quote: ... cells were incubated for 1 hour respectively with the secondary antibodies goat anti-rabbit IgG (H+L) conjugated to Alexa fluor 594 (Molecular Probes A-11012, 1:400); goat anti-mouse IgM conjugated to Alexa fluor 594 (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... sections were again washed in 0.01M PBS and incubated for 1 h at room temperature with the secondary conjugated antibody (1:1000, Invitrogen: goat anti-rabbit Alexa Fluor 488). The sections were washed in 0.01M phosphate buffer ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated for 1 h at room temperature with Alexa Fluor 488-conjugated donkey anti-rabbit secondary antibody (1:200; Invitrogen, A-21206, Waltham, MA, USA). The Gi-DREADD or stained brain sections were mounted on resin-coated slides and dried slightly ...
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bioRxiv - Molecular Biology 2024Quote: ... The slides were washed thrice with PBS and blocked with 5 % milk inside a humid chamber for 1 h before being incubated with α-V5 mouse primary antibody (Thermo Fisher Scientific – 1:400) overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were washed three times in PBS and were incubated in the dark for 1 h at room temperature with goat anti-mouse Alexa Fluor® 488-conjugated antibody (Invitrogen, USA, 1:200 dilution) or goat anti-rabbit Alexa Fluor® 488-conjugated antibody (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... The sample was then washed three times followed by an incubation for 1 h with the following secondary antibodies: goat anti-rat Alexa Fluor 647 antibody (Thermo Fisher, #A78947, 1 µg/ml), goat anti-mouse Alexa Flour 488 antibody (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were resuspended to a density of ∼2.5 × 105 cells and allowed to attach for 3 h in 3 ml Nunc cell culture tubes #156758 (Thermo Fisher Scientific, Waltham, MA, USA) before exchanging the medium into encystation medium ...
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bioRxiv - Immunology 2022Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S ‘2P’ and HexaPro IMAC elution fractions were concentrated to ∼ 1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Immunology 2020Quote: ... and 0.75% w/v 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS) in a hydrated 10K molecular weight cutoff dialysis cassette (Thermo Scientific). S-2P IMAC elution fractions were concentrated to ~1 mg/mL and dialyzed three times into 50 mM Tris pH 8 ...
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bioRxiv - Cancer Biology 2022Quote: ... Pierce premium grade N-hydroxysulfosuccinimide (sulfo-NHS, PG82071) and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, 22980) were purchased from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2022Quote: ... PCR products (1□μg) of desired templates were 3’ end-labeled using a Pierce biotin 3’ End DNA Labeling Kit (Thermo Scientific). The resulting probe reaction mixtures were electrophoresed on a 0.8% agarose gel for 30 min at 100 V and then gel purified with a NucleoSpin Gel and PCR Clean-up kit (Takara Bio USA) ...
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bioRxiv - Immunology 2023Quote: ... viable CD45-CD34+Sca-I- cells were sorted from epidermal single cell suspensions of 1-month-old EGFRΔEgr2 (n=3) and littermate controls (n=3) into Trizol LS Reagent (Thermo Fisher Scientific) using a FACS Aria III ...
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bioRxiv - Microbiology 2023Quote: ... cells were blocked for 1 h in 3% BSA/PBS and incubated with rabbit-anti-GFP (1:50 in 3%BSA/PBS, 200 µl/well, Invitrogen, G10362) for 1 h at 37 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... ES cells were passaged every 3–4 days using Accutase (Thermo Fisher) and used to passage 30 ...
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bioRxiv - Biochemistry 2020Quote: ... blotted for 3–4 s in a Vitrobot Mark IV (Thermo Fisher) at 15 °C and 100% humidity ...
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bioRxiv - Microbiology 2020Quote: ... PobA activity was inhibited with methyl 4-hydroxy-3-iodobenzoate (Fisher Scientific) at a saturating concentration (0.48 mM) ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were grown for 3-4 days in Neurobasal medium (Thermo Fisher) supplemented with 2% FBS (HyClone) ...
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bioRxiv - Biophysics 2021Quote: ... Stained cells were cytospun (Thermo Scientific Cytospin 4 1000rpm for 3 minutes) onto 1.5 thickness coverslips and mounted with ProLongTM Gold mounting medium (Molecular Probes #P36934 ...
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bioRxiv - Molecular Biology 2022Quote: ... iPSC-neurons were incubated with 3 µM Fluo-4 (Life Technologies #F14201) at 37°C for 20 min and imaged directly following washout on an LSM Zeiss 880 inverted confocal microscope using a 63×oil 1.4 numerical aperture objective and a 488-nm argon ion laser ...
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bioRxiv - Genetics 2023Quote: ... Cultures were passaged enzymatically every 3-4 days using 0.25% Trypsin (Gibco) diluted 1:3 in 1X PBS (Gibco) ...
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bioRxiv - Neuroscience 2024Quote: ... Invitrogen) and DiD (1,1’- Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine, 4-Chlorobenzenesulfonate Salt; Invitrogen) crystals were inserted under a stereo fluorescence microscope (MZ10 F ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were passaged every 3 to 4 days using Accutase™ (Gibco) for up to 10 passages.
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bioRxiv - Immunology 2021Quote: ... 7-AAD (Invitrogen), Live/dead fixable viability dye (Invitrogen) ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... 2×109 1-µm Neutravidin-coated yellow-green FluoSpheres (Invitrogen #F8776) were resuspended in 1 mL of 0.1% PBS ...
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bioRxiv - Genomics 2020Quote: ... E-cadherin (rat, Life Technologies 131900 clone ECCD-2, 1:100), Mucin 1 (Muc1 ...
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bioRxiv - Immunology 2021Quote: ... and 1% (v/v) L-glutamine (2 mM final concentration, Gibco). Cells were maintained at 37°C (5% CO2) ...
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bioRxiv - Immunology 2019Quote: ... 1 mM sodium pyruvate and 2 mM L-Glutamine (Thermo Fisher) to a final cell density of 1 × 107 cells per ml ...
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bioRxiv - Biophysics 2019Quote: ... HEK cells were loaded with 1 μM fura-2 AM (Invitrogen) at 37°C for 40-50 min ...
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bioRxiv - Microbiology 2019Quote: ... 1 mM Tris(2-carboxyethyl)phosphine hydrochloride (TCEP-HCl, ThermoFisher Scientific), and 250 μg/mL DNase ...
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bioRxiv - Genetics 2019Quote: ... supplemented with 2 mM glutamine and 1% penicillin/streptomycin (both Invitrogen), and 10% fetal bovine serum ...
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bioRxiv - Immunology 2019Quote: ... 1 μl of WGA-AF488 (2 mg/mL in PBS, Invitrogen) was injected into the iLN of Nav1.8Cre/+ ...
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bioRxiv - Neuroscience 2019Quote: ... supplemented with 2 mM glutamine and 1% penicillin/streptomycin (both Invitrogen), and 10% fetal bovine serum ...