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Citations for Thermo Fisher :
2901 - 2950 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen Corp., USA) was used for counterstaining ...
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bioRxiv - Cell Biology 2024Quote: ... 4’,6-diamiino-2-phenylindole (DAPI; Invitrogen, Cat# D21490) diluted 1:1000 in PBS was applied for 15 minutes at room temperature with plates subsequently washed ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 mL of cold 4% PFA (Thermo Scientific, 28908) in RNAse-free PBS (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI, Life Technologies). Whole-mount stained slices were then washed in PBS and incubated overnight in RapiClear 1.52 ...
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bioRxiv - Cancer Biology 2023Quote: ... with 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen, D3571) 1:1000 in 1% BSA included in the second wash ...
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bioRxiv - Bioengineering 2023Quote: ... and 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI, Invitrogen) staining to visualize the cytoskeleton and nucleus ...
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bioRxiv - Pathology 2023Quote: ... including 4’,6-Diamidino-2-Phenylindole (DAPI) (ThermoFisher, D1306), were prepared according to the manufacturer’s recommendation and applied to each section ...
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bioRxiv - Developmental Biology 2022Quote: ... DAPI (4’,6-diamidino-2-phenylindole; Invitrogen REF: D1306) diluted at 1:1000 in PBT was added to the samples for 10 minutes at room temperature on a rotating wheel followed by a wash in PBT for 10 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 4′,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) was used at 0.2μM.
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bioRxiv - Cancer Biology 2023Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) followed by an appropriate secondary Ab with Alexa Fluor 488 ...
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bioRxiv - Cancer Biology 2024Quote: DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride) (#D1306, Invitrogen)
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bioRxiv - Physiology 2024Quote: ... and 4’,6-diamidino-2-phenylindole (DAPI) (Life Technologies), using Alexa Fluor 594 anti-insulin (Life Technologies ...
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bioRxiv - Immunology 2023Quote: ... stained with Ghost-510 and 2-(N-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 60 µM) (Thermo Fisher Scientific) (30 min at 37 C) ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Immunology 2021Quote: ... biotinylated with 7:1 biotin-to-antibody using EZ-Link Sulfo-NHS-LC-Biotinylation Kit (21435, Thermo Fisher Scientific), was prepared in dilution buffer ...
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bioRxiv - Neuroscience 2019Quote: ... Plasmids pCAG-dsRed and Pak3K297R transfection was performed at 7 d in vitro (DIV) with 1 µg of DNA and 1.5 µl of Lipofectamine (Invitrogen), as described in manufacturer’s manual (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: Cell viability after 1 and 7 days was examined using Calcein AM (Thermo Scientific, Breda, the Netherlands, CCID: 390986) for live cells and propidium iodide (PI ...
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bioRxiv - Microbiology 2022Quote: ... using qScript 1-Step SYBR Green qPCR kit (Quantabio) on a ViiA 7 Real-Time PCR System (Applied Biosystems); 16S rRNA was used for normalization ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were pelleted at 3,000 g, aspirated, resuspended in SYTOX buffer (500 µL 50mM sodium citrate [pH 7], 1 µM SYTOX green (Invitrogen)) ...
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bioRxiv - Genomics 2022Quote: ... mouse pancreatic islets isolated from RipCre-tdTomato mice were trypsinized for 7 minutes with 1 mM of trypsine (Gibco). Dissociated pancreatic cells were directly run into an Influx sorter (Becton Dickinson® ...
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bioRxiv - Cancer Biology 2022Quote: ... 7*103 single cells derived from second-generation spheres were labeled with 1 mM CMFDA (Life Technologies, cat# C7025) for 30 minutes and added on top of the mesothelial monolayer ...
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bioRxiv - Cell Biology 2024Quote: ... The pellet was resuspended in PBS containing 1:1000 (v/v) 7-AAD viability dye (Invitrogen, catalog number A1310). 500 cells per well were sorted on a BD FACSAria III sorter using a 100-micron nozzle in 4-way purity precision mode into an Eppendorf twin.tec 384 LoBind plate containing 1µl of 0.2% DDM ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with 7% Fetal Bovine Serum (FBS) (MilliporeSigma, catalog F4135) and 1% Penicillin/Streptomycin (P/S) (Gibco, catalog 15140163) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2021Quote: ... the Ca+2 indicator Fluo-4-AM (Molecular Probes, Eugene, OR; 2–5 µl of 2 mM dye) were dropped over S1 cortex ...
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bioRxiv - Cell Biology 2019Quote: ... Slides were incubated overnight at 4°C with primary Phospho-Smad 2 (ThermoFisher; #4-244G) antibody diluted 1:200 in blocking buffer ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Developmental Biology 2019Quote: ... Sections were washed 3 times in PBS followed by incubation for 1 hour at RT with AlexaFluor-conjugated secondary antibodies (1:400, Molecular Probes) and 0.15% DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2019Quote: ... samples were washed with washing buffer (0.2%Tween-20/10mg·L-1Heparin/ PBS) for 1 day with 3 times refresh and incubated with dye-conjugated secondary antibodies (1:500, A-21245, Thermo Fisher) in antibody incubation buffer for 1 week at 37 °C ...
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bioRxiv - Genomics 2019Quote: ... were harvested and placed in 3:1 ethanol:glacial acetic acid fixative and then transferred to fresh 3:1 fixative containing 1% Polyvinyl Pyrrolidone (BP431-100, Fisher Scientific, USA). Emerging anthers from the flower buds were isolated and squashed under a 22 × 22 mm glass cover-slip to provide the best anthers for pachytene analysis ...
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bioRxiv - Microbiology 2021Quote: ... the monolayers were overlaid with 3 mL containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Microbiology 2020Quote: ... and were transferred into tubes containing 3 μl of RNase inhibitor solution (1 part 20 U μl−1 RNase Inhibitor [Thermo Fisher Scientific] in 19 parts 0.2% Triton X-100 ...
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bioRxiv - Genomics 2022Quote: ... Samples were washed 3 times with methanol before resuspension in 1 ml PBT containing 1 µL Hoeschst 33342 (Thermo Scientific, USA). After a 10 min incubation at RT ...
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bioRxiv - Microbiology 2022Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Pathology 2024Quote: ... Myofibroblasts were grown on uncovered polystyrene culture plates in 16% FCS in DMEM supplemented with 1% Penicillin-Streptomycin-L-Glutamine and subcultured at a 1:3 ratio using Gibco TrypLETM Express Enzyme (12605010, Fisher Scientific) for cell dissociation ...
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bioRxiv - Bioengineering 2023Quote: ... The barrier function of the Calu-3 monolayer was terminally evaluated via immunofluorescence imaging of tight junction protein 1 (ZO-1 antibody, Invitrogen, 339194) and permeability assays using 500 µL of 2 mg/mL FITC-dextran (4 kDa ...
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bioRxiv - Neuroscience 2023Quote: ... cryoprotected in 30% sucrose solution (for 1 to 3 days) and incubated with AF488 streptavidin (1:1000, Invitrogen, Cat#: S11223, 2390711) in PBS containing 0.3% Triton X-100 (Aladdin ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011). After 1 hr incubation ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody labeling was done at 1:1000 dilution in 3% goat serum/PBST at room temperature for 1 hr using goat-anti-chicken-AF488 (ThermoFisher A11011), goat-anti-rabbit-AF568 (ThermoFisher A11036) ...
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Microbiology 2023Quote: ... and monolayers were overlaid with 3 ml containing a 1:1 mixture of 1.2% oxoid agar and 2X DMEM (Gibco, Carlsbad, CA) with 10% (vol/vol ...
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bioRxiv - Physiology 2023Quote: ... followed by 1 hour incubation in DMEM/3% bovine serum albumin containing 1 μM AlexaFluor 647 conjugated α-bungarotoxin (Life Technologies) at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Cell Biology 2024Quote: ... experiments were grown overnight in SDC at RT and then diluted in SDC and grown at 30°C for >4 h before being treated with either 500 µM 3-indole acetic acid (3-IAA; AID) for 1h or 1 µM estradiol + 1 µM 5-phenyl-IAA (5-Ph-IAA; Fisher Scientific) for 2h (grAID ...
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bioRxiv - Cell Biology 2024Quote: ... suspended in arrest-mixture assay buffer (no ATP) with 3% BSA and 1:400 anti-CARMIL1 antibody (ThermoFisher, 27133-1-AP). After incubation for 2 hr at RT ...
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bioRxiv - Bioengineering 2024Quote: ... 80-90 % of cell cultures were split into a ratio of 1/3 to 1/6 using Tryple Express Enzyme (Gibco, 12604021) and Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Immunology 2021Quote: ... cells were primed with 200 ng/ml LPS for 3 h in Opti-MEM® (Gibco) or DMEM supplemented with 10% FBS before NLRP3 stimulus was used ...
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bioRxiv - Developmental Biology 2021Quote: ... endogenous peroxidase activity was quenched using 3% H2O2 (Cat No. H/1750/15, Thermo Fisher Scientific). Antigen retrieval was performed by treatment of tissue sections with porcine trypsin tablets (Cat No ...
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bioRxiv - Cell Biology 2019Quote: ... 10 mg of total protein lysate were incubated for 3 h with protein A Dynabeads (Invitrogen) and 10 μg of MST2 antibody (ab52641 ...
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bioRxiv - Developmental Biology 2022Quote: ... COCs were washed 3 times and then cultured for 17 h in bicarbonate-buffered αMEM (Gibco) supplemented with 3 mg/mL BSA ...
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bioRxiv - Microbiology 2022Quote: ... Alexa Fluor 488 goat anti-mouse IgG (H+L) and TO-PRO-3 were from Invitrogen. Immunofluorescence and confocal microscopy were performed as described (40).