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Citations for Thermo Fisher :
2951 - 3000 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... for 3 h then stained for CD4 and a LIVE/DEAD Fixable Dead Viability Dye (Invitrogen). Cells were then fixed and permeabilized (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... the chitin preparation was incubated for 3 h with 10 µg/ml polymyxin B (Thermo Fisher), washed by centrifugation ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Bioengineering 2023Quote: B-RBD cells (1 × 107 cells/mL) were combined with 4 μM Fluo-4 AM (Invitrogen, USA, Cat: #F14217) for 20 min at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... One portion was incubated for 16 h at 4°C with Dynabeads (Thermo Fisher Scientific) directly conjugated to anti-CD9 antibody with rotation ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were washed and incubated for 4 h with appropriate Alexa-labelled secondary antibodies (Invitrogen; 1:1000 ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein A Dynabeads (Invitrogen) precoated with the corresponding antibodies ...
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bioRxiv - Plant Biology 2024Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 h in the presence of Protein Transport Inhibitor Cocktail (Thermo Fisher Scientific). Splenocytes from LASgpc-mRNA-LNP-immunized mice were stimulated with the LASgpc peptide cocktail ...
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bioRxiv - Microbiology 2023Quote: ... and then 4 h in the presence of Protein Transport Inhibitor Cocktail (Thermo Fisher Scientific). Cell Stimulation Cocktail (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by a 16-18-h incubation at 4 °C with V5 primary antibody (Invitrogen) diluted 1:50 in blocking buffer ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
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bioRxiv - Cancer Biology 2023Quote: H&E staining for mouse tissues were fixed in 10% formalin (SF98-4, Thermo Fisher) processed in UF pathology core ...
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bioRxiv - Cancer Biology 2020Quote: ... The HDFC was dissolved with a 1:1 mixture of 2 mg/ml collagenase type2 and trypsin (Gibco), and cell numbers were quantified using hemocytometry ...
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bioRxiv - Microbiology 2019Quote: ... Inoculum was removed and 2 mL of overlay media (MEM containing 1% methylcellulose, 10% FBS, 1% GlutaMAX (Gibco), 1% HEPES ...
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bioRxiv - Neuroscience 2022Quote: ... and then switched to neural induction medium (Advanced DMEM/F12 (1:1) supplemented with 2 mM GlutamaxTM (Gibco), 2% B27 without vitamin A (Life technologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... were similarly cultured but in DMEM:Ham’s F12 (1:1) medium with 2 mM glutamine and 10 % fetal-calf serum (Invitrogen). FugeneHD (Promega ...
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bioRxiv - Molecular Biology 2021Quote: ... Thawed CM aliquots were mixed 1:1 with basal media (BM) consisting of 2% B-27 (Gibco 12587001), 10 mM Nicotinamide (Sigma-Aldrich N0636) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were fixed for 1 minute in 1 ml PBS 2% formaldehyde (Thermo-Fisher Scientific, Cat. No. 28906) before being fixed for a further 14 minutes in fresh PBS 2% formaldehyde ...
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bioRxiv - Neuroscience 2024Quote: ... Wisteria floribunda lectin (1:1000, B-1355-2, Vector) was visualized using Streptavidin 568 (1:500, S11226, Invitrogen).
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bioRxiv - Immunology 2024Quote: ... mixing with 2 million CD45.1+ T cells at 1:1 ratio and labeling with CellTrace Violet dye (Invitrogen), into Rag1−/− mice ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were resuspended at 2 x 106 cells/ml in assay medium containing DMEM/F12 (1:1, Gibco) supplemented with 5% horse serum (Invitrogen) ...
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bioRxiv - Developmental Biology 2023Quote: ... and plated 1×106 and 2×106 cells on Geltrex (Gibco, 1:100 DMEM/F12, Thermo Fisher Scientific)- coated 60 mm and 10 cm plates respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor (AF) 647-conjugated streptavidin (1:300 diluted from 2 mg ml−1 stock solution, Invitrogen S32357) was added ...
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bioRxiv - Cell Biology 2020Quote: Cellular ROS production was measured using a CM-H2DCFDA (2’,7’-dichlorofluorescein diacetate) (Life Technologies, USA) assay kit ...
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bioRxiv - Cancer Biology 2021Quote: ... live zebrafish larvae (7 dpf) were incubated at 28°C in 2 mM EdU (Invitrogen, #C10340) in E3 medium for 2 h followed by a further incubation in fresh E3 medium for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... qPCR was performed using 2× Power SYBR green reagents on the QuantStudio 7 Thermocycler (Life Technologies).
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bioRxiv - Molecular Biology 2021Quote: Striatal ROS was marked by 2’ 7’-dichlorodihydrofluorescein diacetate (Molecular Probes™ H2DCFDA (H2-DCF, DCF), ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Fluorescent probes dihydroethidine and 2’,7’-dichlorodihydrofluorescein diacetate were obtained from Molecular Probes (Eugene, Oregon, USA).
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bioRxiv - Cell Biology 2024Quote: ... intracellular ROS and GSH levels in keratinocytes were measured using H2DCFDA (2’, 7’- dichlorodihydrofluorescein diacetate; Invitrogen) and CellTracker Blue (4-chloromethyl- 6.8- difluoro- 7- hydroxycoumarin ...
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bioRxiv - Physiology 2023Quote: ... followed by 7 days of decalcification with Shandon’s TBD-2 (Thermo Fisher Scientific, Waltham, MA, USA) at room temperature ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... reagent at a 4:2:3 ratio of sgRNA/overexpression-construct: pVSVG: psPAX2 in Opti-MEM media (Life Technologies, Thermo Fisher Scientific Inc, Waltham, MA). Viral supernatants were collected 48 hours post-transfection and filtered through a 0.45 μm Nalgene syringe filter SFCA (Whatman ...
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bioRxiv - Developmental Biology 2021Quote: Zebrafish fins or larvae euthanized in 1% Mesab were fixed in 4%PFA/1xPBS and embedded in 1% agarose (CryoStar NX50, Thermofisher) or tissue freezing medium (Leica ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then incubated overnight at 4°C with the following primary antibodies at a concentration of 1:500: mouse anti-V5 (1:500, Invitrogen), rabbit anti-γ-H2AX (1:500 ...
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bioRxiv - Bioengineering 2019Quote: ... overnight at 4°C followed by AlexaFluor-568 (1:500, Invitrogen, A21099 and AlexaFluor-647 antibody (1:500, Invitrogen, A31573) respectively ...
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bioRxiv - Neuroscience 2020Quote: ... (iii) incubated overnight at 4 °C in the following affinity-purified rabbit KV7.2 (residues 1–70; 1:1000, Thermo Scientific), KV7.3 (residues 668–686 ...
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bioRxiv - Microbiology 2020Quote: ... Cell pellets were resuspended in 1 mL of cold 1 X PBS (4°C) containing protease inhibitor (ThermoFisher; cat# 78429) and were transferred into tubes containing lysing matrix B (MP Biomedicals ...
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bioRxiv - Developmental Biology 2022Quote: ... with 0.1% (w/v) Tween20 and incubated overnight with primary antibodies at 4° C: anti-HIF1α (1:500, MA1-16504 Invitrogen); anti-STAT3 (1:1000 ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS containing 0.3 % Triton X-100 and incubated overnight at 4°C with a rabbit anti-Iba1 antibody (1:500, Wako) in PBS containing 1 % FBS (Gibco), 0.1 % BSA (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... Blot sections were probed overnight at 4°C 1:5000 with the ATXN1 antibody 11750 (Servadio et al., 1995) or 1:2500 with H1 antibody (Invitrogen) diluted in 5% milk PBST ...
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bioRxiv - Microbiology 2021Quote: ... clarified transfection supernatant was diluted 1/4 in a 20 μl volume of transfer buffer (Life Technologies Cat# NP0006-1) and applied dropwise to a presoaked 0.45 μm PVDF membrane ...
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bioRxiv - Biophysics 2021Quote: ... or ParB-EDTA buffer (by replacing the 4 mM MgCl2 with 1 mM EDTA) and then incubated with 1 μm diameter magnetic beads (Dynabeads, Myone streptavidin, Invitrogen) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4 °C with primary antibodies (Mouse anti-Cx26, 1:100, Invitrogen; Rabbit anti-Cx26; 1:250, Invitrogen; Rabbit anti-Cx30 ...
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bioRxiv - Pathology 2020Quote: ... The aqueous phase was separated by centrifugation (21,000 g for 15 min at 4 °C) and incubated with isopropanol (1:1 ratio) and 25 μg of Glycoblue (ThermoFisher) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and then stained with HERV-K envelope primary antibodies (1:1000) (Ango) overnight at 4°C and Alexa Fluor 488 secondary antibodies (1:500) (Invitrogen) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... was performed at 30V for 1 hour at 4°C in 1X NuPAGE™ buffer (REF NP0006-1, Life Technologies). After transfer ...
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bioRxiv - Microbiology 2022Quote: Loss of function of NEU1-4 was conducted by transfecting cells with pooled siRNAs (Supplemental Table 1) (Dharmacon) or single siRNAs (Supplemental Table 1) (ThermoFisher) using RNAiMAX (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were first incubated with rat anti-HA (1:100) antibody overnight at 4°C followed by donkey anti-rat Alexa Fluor (AF) 488 (1:500; Invitrogen) for 1.5 h at 20°C before staining for other markers ...