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Citations for Thermo Fisher :
2551 - 2600 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: T98G cells (2 × 105 into 6 well plate) were reverse transfected with siRNAs (Table S3) using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were transfected with 10nM final concentration of control or target siRNAs using Lipofectamine™ RNAiMAX (Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... cells were seeded in the appropriate wells or plates and transfected with 0,2 µM of siRNA using Oligofectamine (Invitrogen) accordingly to manufacturer’s instructions using non-targeting siRNA (siCtrl ...
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bioRxiv - Cancer Biology 2019Quote: ... Transfections for siRNA used Invitrogen’s Lipofectamine RNAiMAX and for plasmid DNA we used Lipofectamine LTX Plus (ThermoFisher, MA, USA). Cells were treated for 24 hours then refed with fresh growth medium for another 48 hours before collection for protein or growth assay in knockdown experiments ...
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bioRxiv - Cell Biology 2019Quote: ... At 90% confluence the cells were transfected with 30 nmol/L siRNA using Lipofectamine RNAi MAX (# 13778, Invitrogen, MA) in OptiMEM according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... For RHBDL4 and OST4 depletion 50 pmol (RHBDL4) or 25 pmol (OST4) ON-TARGETplus SMARTpool human siRNA (Dharmacon) and 2.5 μl RNAimax (Invitrogen) transfection reagent per well of a 12-well plate were used ...
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bioRxiv - Cancer Biology 2020Quote: ... Transfection of HCT116 cells (0.6×106 cells/well in 6-well plate) with siRNA oligonucleotides was performed using Lipofectamine RNAiMAX (Invitrogen).
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bioRxiv - Pathology 2020Quote: ... Transfection with SMARTpool siRNAs targeting Nck1 (L-006354) and Nck2 (L-019547; IDT) was performed using Lipofectamine 3000 (Invitrogen) according to the manufacture’s recommendations ...
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bioRxiv - Immunology 2020Quote: ... HeLa S3 cells were transfected with siRNA oligonucleotides with the use of the Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) and were used for experiments after incubation for 72 h ...
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bioRxiv - Genetics 2021Quote: ... with 12.5 pmol of either non-targeting negative control siRNA showing no homology to the human transcriptome (#4457287, Ambion), or two different siRNAs targeting OAS1 (siRNA-1 or −2 ...
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bioRxiv - Biochemistry 2019Quote: ... Knockdown of PALB2 was performed 18 hours later with 50 nM PALB2 siRNA (Table S4) using Lipofectamine RNAiMAX (Invitrogen). After 5 hours ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were transfected with either a silencer select siRNA with si-SWELL1 (Cat#4392420, sense: GCAACUUCUGGUUCAAAUUTT antisense: AAUUUGAACCAGAAGUUGCTG, Invitrogen) or a non-targeting control silencer select siRNA (Cat# 4390846 ...
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bioRxiv - Pathology 2021Quote: ... Cells were treated with siRNA at a final concentration of 100 nM using Lipofectamine RNAiMAX according to the manufacturer’s instructions (Invitrogen). After 48 h ...
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bioRxiv - Molecular Biology 2020Quote: The transfection of the plasmid DNA and short interfering RNA (siRNA) was performed using polyethylenimine (PEI) and RNAiMAX (Invitrogen), respectively ...
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bioRxiv - Physiology 2021Quote: INS-1 or MIN6 cells were transiently transfected with small interfering RNA (siRNA) using Lipofectamine 2000 (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... For siRNA experimentsHEK293T cells (1.5×105 cells/ml) were seeded in 6-well plates and transfected with 150nM siRNA using 4μl Lipofectamine 2000 (Invitrogen) and 184μl OptiMEM ...
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bioRxiv - Cell Biology 2021Quote: 1.5-2 × 106 cells were seeded 24h before transfection with 45 nM of siRNA using Lipofectamine RNAiMAX (Life Technologies). The following siRNA molecules used against the coding sequence of Pol κ (Polκ ...
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bioRxiv - Genomics 2020Quote: Cells were reverse transfected with 20 nM of the indicated siRNAs using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) and OptiMEM (Gibco) ...
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bioRxiv - Molecular Biology 2021Quote: Small interference (si)RNA-mediated gene knockdown experiments were carried out with cells transfected with 10 nM siRNA (Invitrogen) for 3 days using Lipofectamine RNAimax (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: ... Briefly 24 pmol siRNA was used together with 4 µl of liposomal transfection reagent in 400 µl OptiMEM (Invitrogen) per 3×105 cells and scaled accordingly ...
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bioRxiv - Cancer Biology 2022Quote: Transfection of cell lines with 10 nM small interfering RNAs (siRNAs) was performed using RNAiMAX Transfection Reagent (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: siRNAs purchased from Eurofins MWG Operon or Sigma-Aldrich (Table 4) were transfected into cells using Lipofectamine RNAiMAX (Invitrogen) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Transient transfection was performed using 0.2 μM siRNA or 10 μg Tomosyn-1-V5-RVV or GFP-V5-RVV using Lipofectamine 2000 (Invitrogen) construct at a 1:2 ratio in Opti-MEM ...
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bioRxiv - Microbiology 2022Quote: ... HeLa cells were transfected with respective siRNAs at several concentrations (see figure legends) using lipofectamine RNAiMAX (13778075; ThermoFisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: We used the QuantiGene multiplex system to simultaneously measure the expression of multiple genes in siRNA-treated SHSY5Y cell samples.52,53 Individual reagents and probe sets were purchased from ThermoFisher. Probes were directed against ...
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bioRxiv - Microbiology 2022Quote: ... Huh7 cells were transfected with 10 nM siRNA using Lipofectamine RNAiMAX according to the manufacturer’s protocol (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... cells were plated in six-well plates and transfected with 20 nM siRNA (siNeg, siHNRNPH1, or siFLI1) complexed with 4.5 µL/well Lipofectamine RNAi-Max (Invitrogen) and harvested 48 hours post-transfection ...
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bioRxiv - Molecular Biology 2022Quote: ... RNAiMAX (9µl/well) and siRNA (100pmol/well) was diluted in Opti-MEM medium (Cat#31985062, Thermo Fisher Scientific, USA). The mixture of RNAiMAX and siRNA was then added to the well for 24hr ...
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bioRxiv - Molecular Biology 2022Quote: ... each well was transfected with 200 ng of each sgRNA/Cas9 plasmid plus 3.75 pmol of siRNA using Lipofectamine 2000 (Thermofisher), with 0.5 ml of antibiotic-free media ...
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bioRxiv - Systems Biology 2023Quote: ... siRNA transfections in 12-well plate for measuring changes in secreted factors were carried out using Lipofectamine RNAiMax (Invitrogen) according to manufacturer instructions.
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bioRxiv - Biochemistry 2024Quote: ... or SK-N-BE(2) cells were transfected with the siRNAs using Lipofectamine RNAiMAX Transfection Reagent (ThermoFisher Scientific, #13778030) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The procedure was the same as described above except that the siRNA reverse-transfection was performed with RNAiMax (Invitrogen) and the medium change was performed 6h later ...
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bioRxiv - Molecular Biology 2024Quote: ... Cambridge) and scramble siRNA were transfected into Neplic cells cultured in 12-well plates using Lipofectamine 3000 reagent (Invitrogen). The medium was not replaced ...
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bioRxiv - Pathology 2024Quote: ... A final concentration of 30 nM of each siRNA was transfected into cultured cells using Lipofectamine 2000 (Life Technologies), as described previously23.
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bioRxiv - Neuroscience 2024Quote: ... Before transfection the siRNAs were mixed with OptiMEM medium (Thermo) and Lipofectamine RNAiMax (2 µl/ml; Thermo Fisher Scientific) and incubated at RT for 20 min.
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bioRxiv - Immunology 2024Quote: ... Lipofectamine RNAi max and siRNA were mixed in Opti-MEM reduced serum medium (#31985-070, Thermo Fisher Scientific, USA) and incubated at room temperature for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were transfected with a total of 20 nM siRNAs using the Lipofectamine RNAiMAX transfection reagent (Cat# 13778075, Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2023Quote: ... the media was replaced with Opti-MEM containing Ambion Silencer-Select ERK3 siRNA (5 pmol, catalog number: 4390771 ID: S78451) and Lipofectamine 2000 (2µl, Invitrogen) and cells incubated for 19 h ...
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bioRxiv - Molecular Biology 2022Quote: Transfections in this study were carried out with 20 nM of siRNA using Lipofectamine RNAiMAX and performed according to the manufacturer’s protocol (ThermoFisher). Transfection media was removed the following day after transfection and cellular material was harvested 72 hours after transfection ...
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bioRxiv - Cell Biology 2023Quote: ... The siRNA-mediated knockdown of proteins was performed for 144 h or 168 h using Lipofectamine RNAiMAX (Invitrogen, 13778150) diluted in Opti-MEM (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... the cells were harvested and transfected with either 20 nM non-target control siRNA or 20 nM AMPKα1 (both Dharmacon) or FoxO3 (Invitrogen) siRNA using Neon Transfection System (Invitrogen ...
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bioRxiv - Immunology 2023Quote: DCs were transfected with control or lncRNA-specific siRNA using Lipofectamine 2000 (Invitrogen-Life Technologies Corporation, Carlsbad, CA, USA), as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: For siRNA-mediated knock-down, siRNA (0.4 nmol ml−1 Hela, HeLa S3 or MEFs) was electroporated using the Neon Transfection System (Invitrogen) according to cell line specific instructions of the manufacturer ...
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bioRxiv - Microbiology 2022Quote: siRNA silencing was performed by transfecting 2.5 pmol of each siRNA per well in a 12-well plate into the RAW264.7 cells using Lipofectamine RNAi Max (13778150, ThermoFisher) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Transfection of cell lines with specific siRNAs was carried out using Lipofectamine (cat#11668-018, Invitrogen, Waltham, MA, USA) or DharmaFECTTM (cat#T-2022-02 ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 uL solutions of siRNA were prepared at 10X concentration in PBS and subsequently mixed with OptiMEM (Gibco 31985062) and Lipofectamine RNAiMax Transfection Reagent (Invitrogen 13778030 ...
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Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeatsbioRxiv - Molecular Biology 2023Quote: ... Cells were then transfected with siRNA at 1 nM/well in a mixture with Lipofectamine RNAiMax (Thermo Fisher, 13778075) following the manufacturer’s recommended protocol on the same day while seeding the cells ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting human UBE3A or human MASTL (Integrated DNA Technologies) was transfected into cells using Lipofectamine RNAi MAX (Invitrogen). A non-targeting control siRNA was used as a control ...
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bioRxiv - Molecular Biology 2023Quote: siRNA was transiently transfected into HUVECs using Lipofectamine RNA iMAX transfection reagent (Thermo Fisher Scientific, Invitrogen, Carlsbad, CA, USA), according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2022Quote: ... After transfection with Lipofectamine 3000 and the indicated plasmids or siRNAs for 48 h according to the manufacturer’s instructions (Invitrogen), the transfection efficiency was assessed by Western blotting (WB ...