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Citations for Thermo Fisher :
2451 - 2500 of 3282 citations for CEPT1 siRNA since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... U2OS cells were transfected with a mixture of three Stealth™ siRNA duplex oligonucleotides (Invitrogen; HSS123763, HSS123765, HSS182809) at a concentration of 10 nM each ...
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bioRxiv - Cell Biology 2022Quote: Two separate human ASAH1-siRNAs (50 nM) (Dharmacon,) were transfected into 621-101 cells using Lipofectamine RNAiMAX (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Molecular Biology 2024Quote: RNA interference (25nM for siRAD51, 60nM for the rest siRNAs) was achieved with Lipofectamine RNAiMax (Life technologies, 13778075) in OPTI-MEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: ... Block-iT Alexa Fluor Red Fluorescent Control siRNA was used as non-targeting negative control (Life Technologies, #14750100). Cells were grown in 60 mm cell culture dishes in medium supplemented with 2% FBS overnight and transfected with 62.5 pmol of the indicated siRNAs applying Lipofectamine RNAiMAX (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: ... HeLaK and Hek293T cells were transfected with with 50-100 nmol siRNA duplex (Eurofins Genomics) using RNAiMax (Invitrogen) or a calcium phosphate-based method ...
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bioRxiv - Molecular Biology 2024Quote: ... siRNA knockdown was carried out following the protocol from Lipofectamine™ RNAiMAX transfection (Invitrogen, catalog no. 13778-100). Cells were harvested 48 hours post transfection and using the protocol as described above ...
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bioRxiv - Neuroscience 2024Quote: ... The expression of genes of interest was silenced in HeLa cells using Stealth RNAi siRNA (Thermo Fisher Scientific) in Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific ...
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bioRxiv - Pathology 2024Quote: Small interfering RNA (siRNA) targeting NFIX and negative control oligonucleotides were purchased from Invitrogen (#1299003, Carlsbad, CA, USA). Three independent NFIX-specific siRNAs were used in this study ...
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Genomic dissection and mutation-specific target discovery for breast cancer PIK3CA hotspot mutationsbioRxiv - Genomics 2024Quote: ... cells were treated with three different commercially validated AREG targeting siRNA (Ambion, see oligo sequences on Table S5), a negative control siRNA (Invitrogen ...
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bioRxiv - Cell Biology 2024Quote: Gene silencing of ALIX was performed in mCCD cells using siRNA and Lipofectamine® 3000 (Thermo Fisher Scientific). mCCD WT cells were seeded in DMEM/F12 in 6-well plates at 70-80% confluency ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 6 nM siRNA mixed with OptiMEM reduced serum medium (Thermo Fisher Scientific, Cat# 31985062) using Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... or untargeted control siRNA (Dharmacon) using DharmaFECT 4 Transfection Reagent in Opti-MEM media (Life Technologies, Carlsbad, CA), according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA transfection was performed at a final concentration of 10 nM using Lipofectamine RNAiMAX transfection reagent (Invitrogen, 13778500) and Opti-MEM (Gibco ...
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bioRxiv - Cell Biology 2023Quote: 72 h after siRNA depletion cells were lysed directly in 1x LDS sample buffer (cat#: NP0007, Thermo Fisher) with β-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: SK-N-SH cells were first transfected with 20 nM of siRNA against TSG101 using Lipofectamine RNAiMAX (Invitrogen). At 48 h post-transfection of siRNA ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cells were transiently transfected with 10nM siIRX4_PEP1 siRNA (Invitrogen by Life Technologies, cat no # 1299001) using Lipofectamine® RNAiMAX transfection Reagent (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Ten microliters of 20 μM siRNA was introduced into BMMCs (5 × 106) by a Neon Transfection System (Invitrogen) set at Program #5 using a Neon 100 μL Kit.
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bioRxiv - Molecular Biology 2023Quote: ... The siRNAs used for silencing target candidate proteins were purchased from Silencer Select (Ambion, Life Technologies, CA, USA) (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2022Quote: ... Transfection of siRNA at a final concentration of 20 nM was performed using Lipofectamine 3000 (Invitrogen, CA, USA) according to the protocol recommended by the manufacturer.
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bioRxiv - Cell Biology 2023Quote: ... The siRNAs were transfected into HeLa cells stably expressing Halo–mGFP–Golgi using Lipofectamine RNAiMAX (13778-150; Invitrogen), in accordance with the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Cell Biology 2023Quote: Cells were suspended in growth media and added to dishes with 50 pmol pooled siRNA and RNAiMAX (Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: HeLa cells were transfected with control or FGF18 siRNAs (Dharmacon SO-2989166G) using Lipofectamine RNAiMAX (Thermo Fisher 137780). The medium was changed 24 hours after transfection ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection of siRNA and miRNA inhibitors were carried out by using Lipofectamine RNAiMax (Thermo Fisher Scientific, Meerbusch, Germany) in accordance with the manufactureŕs instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.5 × 105 cells were initially transfected with 50 nM siRNA using Lipofectamine RNAiMAX following the manufacturer’s protocol (Invitrogen). After 48h of incubation ...
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bioRxiv - Microbiology 2023Quote: ... 30 nM of siRNA was transfected into the human macrophages using Lipofectamine RNAiMAX transfection reagent (Thermo Fisher Scientific) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... Three custom stealth small interfering RNA (siRNA) were used to silence galectin-9 (LGALS9HSS142807, LGALS9HSS142808 and LGALS9HSS142809) (Invitrogen). Equal amounts of the siRNA ON-TARGETplus non-targeting (NT ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cells were transiently transfected with 10nM siIRX4_PEP1 siRNA (Invitrogen by Life Technologies, cat no # 1299001) using Lipofectamine® RNAiMAX transfection Reagent (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... keratinocytes were transfected with 10 nM negative control or E-cadherin siRNA using Lipofectamine RNAiMAX (Thermo Fisher Scientific) in OptiMEM (Thermo fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Reverse transfection of siRNAs was carried out using RNAiMAX according to the manufacturer’s instructions (Invitrogen Thermo Fisher Scientific). Briefly ...
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bioRxiv - Genetics 2023Quote: ... then treated with siRNA or scramble control to a final concentration of 20nM with RNAiMax (Invitrogen, Carlsbad, CA). The siRNAs for PDGFD were purchased from Origene (SR312885) ...
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bioRxiv - Immunology 2023Quote: ... with siRNA delivered at a concentration of either 20 or 50 nM in serum-depleted medium (OptiMEM; Invitrogen) as per the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2022Quote: ... cells were harvested 4 days post siRNA transfection using the Power SYBR Green Cells-to-CT kit (Invitrogen). IDT PrimeTime qPCR Primers for MORC3 and SETDB1 were used and expression levels were normalized to 18S ...
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Deciphering the gene regulatory circuitry governing chemoresistance in Triple-Negative Breast CancerbioRxiv - Cancer Biology 2023Quote: ... siRNA at a final concentration of 5 pmol was diluted in 45 μL of Opti-MEM (Gibco, 31985047) and 2.25 μl of Lipofectamine RNAiMAX was diluted in 45 μl of OPTI-MEM ...
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bioRxiv - Microbiology 2023Quote: ... Cells were transfected with 15 nM siRNA (AP2M1: Thermo Fisher Scientific # 1299001, HSS101955; SCR: Thermo Fisher Scientific #12935300) with 3.33 µL Lipofectamine RNAiMax (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... The siRNAs used for silencing target candidate proteins were purchased from Silencer Select (Ambion, Life Technologies, CA, USA) (Supplementary Table 1) ...
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bioRxiv - Molecular Biology 2023Quote: ... Small interfering RNAs (siRNAs) (Table S5) were transfected into cells using Lipofectamine RNAiMAX reagent (13778-075, Thermo Fisher) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... Three custom stealth small interfering RNA (siRNA) were used to silence galectin-9 (LGALS9HSS142807, LGALS9HSS142808 and LGALS9HSS142809) (Invitrogen). Equal amounts of the siRNA ON-TARGETplus non-targeting (NT ...
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bioRxiv - Physiology 2023Quote: ... VSMC grown at 70% confluence were transfected overnight with 30 nM siRNA using lipofectamin RNAiMax (Invitrogen, 13778-075). After washing ...
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bioRxiv - Genomics 2024Quote: MEFs were transfected with 200 nM FAK or p130Cas siRNAs using Lipofectamine 2000 reagent (cat. no. 11668019, Invitrogen) in Opti-MEM (cat ...
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bioRxiv - Molecular Biology 2023Quote: ... siRNAs for ALKBH5 (1; s29686 and #2; s29688) and non-targeting control (4390844) were obtained from Thermo Fisher Scientific and transfected using DharmaFECT 4 Transfection Reagent (Horizon Discovery ...
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bioRxiv - Cancer Biology 2023Quote: EIF4A3 expression was silenced in the cell lines using specific predesigned small interference RNAs (siRNA; #138378, ThermoFisher Scientific). A Negative Control siRNA was also used (#4390843 ...
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bioRxiv - Cancer Biology 2023Quote: ... cell lines were transfected using a mix of 30 nM concentration of siRNA and RNAiMax lipofectamine (ThermoFisher Scientific), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded in 6-well plates directly with the siRNA/transfection mix: 3µl of LipoRNAiMax (Life Technologies), 500µL of OptiMEM (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equivalent quantities of siESR1 or scramble siRNA (scRNA) were forward transfected following the Lipofectamine RNAiMAX (ThermoFisher Scientific #13778150) protocol according to manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... Cells were transfected with siRNA oligomer (final concentration 50 nM) mixed with Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) in serum-reduced Opti-MEM (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Cells were first reverse transfected with siRNAs at a final concentration of 10 nM using Lipofectamine RNAiMAX (Invitrogen) and seeded in 96-well glass-botom imaging plates ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: Transfections of hFC with siRNAs was performed using Lipofectamine RNAiMAX and following the supplier’s procedure (Thermo Fisher Scientific). The following siRNAs were used at a final concentration of 40 nM for 48 h ...