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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and 4’,6-Diamidino-2-Phenylindole (DAPI, Invitrogen). Confocal analyses of stained slides were performed using a TCS SP8 Laser Scanning Spectral Confocal Microscope (LEICA Microsystems) ...
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bioRxiv - Molecular Biology 2019Quote: ... DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher) and ActinRed™ 555 ReadyProbes™ (Molecular Probes ...
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bioRxiv - Immunology 2020Quote: ... Puromycin (2-4 μg/ml, Thermo Fisher, A113803) was used for TRIM25-knockdown HEK293T cell selection ...
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bioRxiv - Neuroscience 2022Quote: DAPI (4′,6-diamidino-2-phenylindole, D1306, Invitrogen) staining was performed by incubation at 1:500 for 10 min in DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... DAPI (4’, 6-diamidino-2-phenylindole, ThermoFisher Scientific) was applied to samples at a concentration of 0.1μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D3571) was added for 5 minutes in the dark ...
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bioRxiv - Bioengineering 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher) was added for 30 min at room temperature before the secondary antibodies were washed out in PBS 3x for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... DAPI (4-6-diamindino-2-phenylindole; Molecular Probes) was used to detect DNA ...
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bioRxiv - Bioengineering 2022Quote: ... DAPI (4’ −6’ -diamino-2-phenylindole, dilactate; Invitrogen) was used for nuclear staining ...
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bioRxiv - Molecular Biology 2024Quote: 4’,6-diamidino-2-phenylindole - DAPI (Invitrogen, R37606); Ulex europaeus agglutinin-I ...
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bioRxiv - Microbiology 2019Quote: ... Caspase-like activity was measure with CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen), a nucleic acid-binding dye that harbors the caspase-3/7 cleavage sequence ...
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bioRxiv - Immunology 2021Quote: ... Cells were then resuspended in 10 µM CellEvent Caspase 3/7 activity reporter in PBS (Invitrogen, #C10723) and incubated for 30 minutes at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were passaged every 3-7 days as single cells using TrypLE™ Select CTS™ (Gibco). The ROCK inhibitor Y27632 (Fujifilm ...
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bioRxiv - Microbiology 2020Quote: ... For detection of effector caspase activity 20 µM CellEvent Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific) was added to the infected cells prior to imaging ...
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bioRxiv - Cancer Biology 2019Quote: ... Taqman® assays (Supplementary table 3) and QuantStudio™ 7 Flex Real Time PCR system (ThermoFisher, USA), and relative expression levels determined using QuantStudio™ 7 Real Time PCR software.
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bioRxiv - Neuroscience 2019Quote: ... unc-7 rescue plasmids were made using the Multisite Gateway® 3-Fragment Vector Construction Kit (Invitrogen). A 1078bp mec-4 promoter fragment (as previously used ...
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bioRxiv - Cell Biology 2021Quote: ... MEF cells were supplemented with CellEvent™ Caspase-3/7 green detection reagent (Life Technologies, Darmstadt, Germany) following manufacturer’s instructions before aldehyde fixation ...
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bioRxiv - Neuroscience 2023Quote: ... 30 microliters of the apoptosis detection reagent (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermofisher, C10723) was added to each well and the slide returned to the incubator for another 60 minutes ...
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bioRxiv - Neuroscience 2023Quote: Dead and apoptotic cells were detected using the CellEvent Caspase-3/7 Kit (#C10423, Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2020Quote: ... 3) latrunculin-b (Lat-B; 2 μM; Fisher Scientific), 4 ...
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bioRxiv - Biochemistry 2020Quote: ... 3 mM 2-deoxy-D-glucose (2DG; ACROS Organics), 2 μM PERK inhibitor (PERKi ...
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bioRxiv - Plant Biology 2022Quote: ... 2–3 μg were treated with Turbo DNase (Ambion). RNA was circularized using T4 RNA ligase and reverse transcription was performed with the Superscript III (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... glass (2 gm, 3 mm bead diameter; Fisher Scientific) and polystyrene (24-well plates ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuolar membranes were stained with 1 μL of 1 mg/mL solution of FM 4-64 (T3166; Invitrogen) in DMSO added at the beginning of incubation of cells in YPD medium ...
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bioRxiv - Cell Biology 2024Quote: ... AlexaFluor 488 anti-rabbit (1:1000, green) and 568 anti-mouse (1:1000, red) (both Invitrogen; Table 4) secondary antibodies were also diluted in 1% BSA and 10 % goat serum in TBS and incubated in the dark for 45 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... 4 μL Tris pH 6.5 1 M and 1 μL Proteinase K (20 mg/ mL, Thermo Fisher Scientific) and incubated shaking for 3 hours at 45°C ...
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bioRxiv - Bioengineering 2024Quote: ... Primary antibodies were incubated at 4°C overnight (Abcam, ab138501, 1:1,000; Thermo Fisher Scientific, AM4302, 1:5,000). Horseradish peroxidase-conjugated secondary antibodies were incubated for 1 hr at room temperature (Abcam ...
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bioRxiv - Physiology 2020Quote: Caspase-3-like activity was quantified using the EnzChek Caspase-3 Assay kit #1 (Molecular Probes, Eugene, OR, USA). Tissue samples were thawed on ice for 5 min ...
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bioRxiv - Cell Biology 2023Quote: ... for 30 min at room temperature using 10 mM Sodium Acetate [pH 5.0] buffer containing 5μg/ml 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC, Thermofisher, 22980) as coupling agent ...
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bioRxiv - Biophysics 2021Quote: ... the samples were incubated with the appropriate secondary antibody (polyclonal goat anti-mouse IgG1, 1:500, ThermoFisher A21124; polyclonal goat anti-rabbit IgG H&L, 1:500, ThermoFisher A11011, Waltham, USA) diluted in blocking solution for 45 min at room temperature ...
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bioRxiv - Systems Biology 2022Quote: ... The synthesized product was digested by incubation for 1 h at 37 °C with 1 ul of FastDigest FspAI (ThermoFisher cat. no. FD0054) and 1 ul of FastDigest PmeI (ThermoFisher cat ...
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bioRxiv - Immunology 2021Quote: ... Slides were washed and then incubated for 1 h with Alexa Fluor 647 donkey goat anti-rabbit IgG (Life technologies #A21244, 1:500). NucBlue™ Live Cell Stain (Thermo Fisher #R37605 ...
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bioRxiv - Pathology 2020Quote: ... sections were incubated for 1 h with secondary antibody (Alexa Fluor® 488 goat anti-rabbit IgG (ThermoFisher Scientific, A-11008, 1:200), or Alexa Fluor® 594 goat anti-rabbit IgG (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for 1 h with secondary antibody labeled with Alexa 488-labeled goat anti-rabbit IgG (1:200; Molecular Probes, Eugene, OR) at 25°C in the dark ...
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated with secondary antibodies for 1 h at room temperature and counterstained by DAPI (1:1000, Thermo Scientific™, Catalog #:62248), coverslipped with EverBrite mounting medium (Biotium ...
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bioRxiv - Neuroscience 2022Quote: ... The whole retinas were incubated at RT for 1 h with Lectin PNA conjugated to Alexa Fluor 647 (1:50, L32460, Life Technologies, Carlsbad, CA) in blocking solution ...
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bioRxiv - Systems Biology 2022Quote: ... The synthesized product was digested by incubation for 1 h at 37 °C with 1 ul of FastDigest BamHI (ThermoFisher cat. no. FD0054) and 1 ul of FastDigest SbfI (ThermoFisher cat ...
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bioRxiv - Neuroscience 2022Quote: ... sections were rinsed 5 x 5 mins and 1 x 1hr before corresponding secondary antibodies along with the nuclear stain Hoechst33342 (1:5000; Molecular Probes; H-3570) were incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... coverslips were incubated for 1 h at RT with appropriate secondary antibodies conjugated to Alexa Fluor 488 or 594 (Molecular Probes, ThermoFisher 1:500) diluted in 0.1% BSA ...
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bioRxiv - Biochemistry 2019Quote: ... non-fat dry milk in Tris buffered saline with Tween-20 (TBST) buffer) for 1 h and then incubated overnight with Anti-V5-HRP antibody (1:10,000; Thermo Fisher Scientific, Langenselbold, Germany) in another blocking buffer (0.25 % (wt/vol ...
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bioRxiv - Cell Biology 2019Quote: Equal numbers of cells were incubated for 1 h at 37 °C with either 1 μM of the membrane dye DiI (Invitrogen, Thermo Fisher Scientific) or 1 μM calcein-AM (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... 2012) (a gift from Andrew Jackson) at 1:300 and rabbit anti sheep IgG (H+L) secondary link antibody (Invitrogen #31240 1:4000) following Leica BondRx standard IHC protocols ...
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bioRxiv - Neuroscience 2021Quote: ... tissues were washed with PBS and were incubated with the following secondary antibodies for 1 h at room temperature: donkey anti-goat Alexa Fluor 488 (1:100; Invitrogen, A32814, RRID: AB_2762838), donkey anti-chicken Alexa Fluor 488 (1:250 ...
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bioRxiv - Cell Biology 2021Quote: ... The filtrate was enzymatically digested for 1 h with 500 μg ml-1 Collagenase type II (Cat no.: 17101-015, Gibco, Grand Island, NY)) ...
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bioRxiv - Microbiology 2020Quote: ... and blots were incubated for 1 h with a 1:1,000 dilution of anti 6X-His Tag rabbit antibodies (Thermo Fisher Scientific, Massachusetts, USA) followed by a 1 h incubation with a 1:3,000 dilution of goat anti-rabbit IgG coupled to HRP secondary antibodies (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... diluted 1:3000 (PBS milk 1% -Tween 0.1%) for 1 h at 25°C and detected using 1-Step(tm) Ultra TMB-ELISA Substrate Solution (Thermo Fisher Scientific, Waltham, MA). Plates were read using Cytation5 (BioTek ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were incubated 1 h at 37 °C with secondary donkey anti-rabbit antibody conjugated to Alexa Fluor 488 (1:200, Invitrogen, Carlsbad CA, USA) in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... bound antibody was detected by incubation for 1 h with secondary antibodies conjugated with Alexa Fluor 488 (1:200, Thermo Fisher Scientific, USA) or Alexa Fluor 546 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000) and then incubated with secondary antibodies Anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 conjugated (Invitrogen, Cat#A11070, 1:1000), Anti-Rabbit IgG (H+L ...