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Citations for Thermo Fisher :
2501 - 2550 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPROPANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed thrice with PBST and incubated for 1 h with secondary antibodies (described below) and AlexaFluor-568 phalloidin (1:500; Life Technologies, Waltham, MA), and/or 1 µM SiR-647-SNAP-dye (New England Biolabs ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by 1 h incubation at 25 °C in a light-tight box with Streptavidin-Cy3 (1 μg/mL, Thermo Fisher Scientific, 10768023) and fluorescent secondary antibody Alexa Fluor 488 donkey anti-mouse (Invitrogen ...
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bioRxiv - Developmental Biology 2023Quote: ... muscle slides were incubated for 1 h at RT with appropriate secondary antibodies (1:500, Alexa Fluor® goat 488 or 680, Thermo Fisher Scientific). After 3 × 5 min washing with PBS ...
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bioRxiv - Microbiology 2023Quote: ... cells were incubated for 1 h with 488 (FLAG) and 594 (LCMV NP) Alexa Fluor antibodies (Life Technologies; 1:500 in blocking buffer), then washed four times with PBS.
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bioRxiv - Cell Biology 2022Quote: ... for 1 h at room temperature the membranes were probed overnight with primary antibodies mouse anti-HP1γ (1:2,500) (Thermo Fisher Scientific #MA3-054), rabbit anti-TRF1 (gift from Titia de Lange ...
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bioRxiv - Cancer Biology 2023Quote: ... Sections were then incubated with the following horse radish peroxidase (HRP)-conjugated secondary antibodies for 1 hour at room temperature: goat anti-mouse IgG (H+L) (Invitrogen, 626520, 1:2000) and goat anti-rabbit IgG (H+L ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained for 1 h at 37°C with Alexa Fluor 488-labeled chicken anti-rat IgG (Invitrogen, A21470, 1:300) and Alexa Fluor 546-labeled goat anti-mouse IgG (Invitrogen ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... Brain slices were then incubated for 20 h at RT with primary antibodies (chicken anti-GFP, 1:1000, ab13970, Abcam; rat anti-mcherry, 1:1000, Thermo Fisher Scientific, M11217) in PBS-based blocking solution containing 0.1% TX ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
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bioRxiv - Biophysics 2021Quote: ... The filtered lysate was diluted 1:2 in purification buffer UA (8 M Urea, 50 mM sodium acetate [Acros Organics, Carlsbad, CA], pH 4). The protein was then purified using a cation exchange HiPrep SP HP 16/10 (Cytiva ...
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bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 2% BSA solution in PBS for 1 h followed by overnight incubation at 4°C with primary antibodies diluted in 2% BSA blocking solution: mouse monoclonal [1F7G5] anti-CKS2 (1:100 dilution; Thermo Fisher Scientific; 37-0300), rabbit polyclonal anti-P63 (p63α ...
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bioRxiv - Biophysics 2021Quote: ... Infrared exposure levels for INS were selected based on their ability to elicit dynamic calcium responses (>2% increase, dF/F) in NG108 cells loaded with a calcium dye (Fluo-4-AM at 1 μM, ThermoFisher, St. Louis, MO, USA). Radiant exposures for no stimulation ...
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bioRxiv - Molecular Biology 2023Quote: ... CKY2038 was grown to mid-log (1-2×107 cells/mL) in SC liquid media and added to the ConA treated perfusion chamber gasket (ThermoFisher, 4 chamber: 19 × 6mm) for treatment at indicated conditions (SC+1% DMSO ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... After several wash and centrifugations, nuclei were suspended in a solution (phosphate=buffered saline containing 1% 4’, 6-diamidino-2-phenylindole (DAPI) (Molecular Probes, Eugene, OR, USA), and nuclei density was determined in a hemocytometer ...
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bioRxiv - Cell Biology 2022Quote: ... The medium was replaced at day 1 to remove non-adherent cells and afterwards every 2-3 day with DMEM/10% FBS/1% penicillin streptomycin (PS) (Gibco, USA, catalog number 15140-122). These cells were passaged serially when cells reached to 80-100 % confluency or when proliferation essentially ceased by using 0.25% Trypsin/EDTA (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... Patient-derived primary cell line GCDX62 was maintained in a 3:1 mixture of Keratinocyte-SFM (KSF; Thermo Fisher Scientific; supplemented with 2% (v/v) FCS ...
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bioRxiv - Immunology 2023Quote: ... Purified T cells were activated for 1 to 3 days with Human T-Activator anti-CD3/CD28 dynabeads at a 1:2 bead-to-cell ratio (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). Prior to analyses ...
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bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: ... an internal solution containing 1 mM Fura-2 (K5-Fura-2, cell impermeable, Invitrogen F1200), 140 mM KCl ...
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bioRxiv - Microbiology 2020Quote: ... upon which 1 μg of yeast RNA and 10× structure buffer (0.1 M Tris at pH 7, 1 M KCl, 0.1 M MgCl2; Ambion) were added ...
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Biochemistry 2021Quote: ... ice cold PBS at 2e6 cells/ml and stained with 7-AAD viability dye (1 ug/mL, Invitrogen). Cell sorting was done on a FACSAria III instrument ...
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bioRxiv - Developmental Biology 2022Quote: ... together with its IgG2a kappa PE/Cyanine 7-Isotype control (1:500, Thermo Fisher Scientific, 25-4321-81), CD31-APC with human specificity for HUVEC experiments (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... washed with 1 x PBS (pH 7) and subsequently labelled with 25 μg/ml AF488 C5 Maleimide (Invitrogen) for 10 min in the dark followed by at least two additional washing steps ...
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bioRxiv - Developmental Biology 2024Quote: ... HPC-7 cells were maintained at density of 1-10 x105/ml in Iscove’s modified Dulbecco’s medium (Invitrogen) supplemented with 50-100 ng/ml of mouse stem cell factor (Gemini Bio-Products) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were treated with RSL3 for 2 h or IKE for 6 h together with the indicated compounds before BODIPY 581/591 C11 (Thermo Fisher) was added in a final concentration of 2 μM per well and incubated for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... slices were incubated for 2 h at room temperature in secondary antibody goat anti-rabbit IgG (H+L) Alexa 647 (Invitrogen, A32733) diluted 1:500 in PB 0.1 M containing 0.3% Triton X-100 and 3% NGS.
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bioRxiv - Physiology 2024Quote: ... then incubated at RT for 2 h with goat anti-Rabbit IgG (H+L) Alexa Fluor Plus 488 (ThermoFisher, cat#A32731) and goat anti-guinea pig IgG (H+L ...
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bioRxiv - Evolutionary Biology 2022Quote: ... pseudonana expressing VHAB-eGFP were incubated with the acidotropic pH stain 2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-aminocarbamoyl)methoxy)phenyl)oxazole (PDMPO) (LysoSensor YellowBlue DND-160; Life Technologies) at a final concentration of 0.125 μM without washing in F/2 ...
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bioRxiv - Physiology 2024Quote: ... only 4 wells of cells were incubated in DMEM with 4 μM fura-2/AM (fura-2/AM, Molecular Probes, USA) at 37° C in the dark for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... Total cell extracts were incubated for 3 h with 20 μl protein A Dynabeads (Invitrogen) and 2 μg of RASSF1A antibody (Atlas ...
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bioRxiv - Bioengineering 2019Quote: ... for 16 h at 4 °C followed by Alexa Fluor-568 antibody (Life Technologies). Images were acquired using a Nikon Eclipse TI A1RSI laser scanning confocal microscope.
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bioRxiv - Molecular Biology 2020Quote: ... Oligonucleotides were transfected 4 h after the plasmid using Lipofectamine™ 2000 (ThermoFisher, #11668027) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 4 h post transfection the medium was replaced with DMEM (Thermo Fisher Scientific, A14430) containing 0.2 % BSA ...
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bioRxiv - Microbiology 2024Quote: ... the RNA was then reverse transcribed for 4 h using SSIV (Thermo Fisher Scientific) with a set of reverse SARS-CoV-2 primers (Supplementary Table 2) ...
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bioRxiv - Physiology 2022Quote: ... for 2 h at 120V and transferred to nitrocellulose membranes using the iBlot 2 mini-stacks (IB23002; Invitrogen) according to the manufacture’s guidelines ...
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bioRxiv - Biophysics 2021Quote: ... and HA-mUNC79 plasmids or their various mutants (in a modified BacMam expression vector 27) at a ratio of 2:1:1:1 using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 24 hours before recording ...
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bioRxiv - Immunology 2019Quote: ... in a 1:1 beat to T-cell ratio and cultured in 2 ml (24WP) or 1 ml (48WP) RPMI 1640 (Thermo Fisher, 21875) supplemented with 10% Fetal Bovine Serum ...
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bioRxiv - Cell Biology 2022Quote: HUVECs expressing PIK3CA p.H1047R or PIK3CA wt were transfected with a Silencer Select siRNA targeting to TGFA (ID: s14053) or negative control siRNAs (#1 and #2 mixed in ratio 1:1; all siRNA oligonucleotides from Thermo Fisher Scientific) as previously described (58) ...
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bioRxiv - Physiology 2023Quote: ... Cells were grown on DMEM/Hams F12 with 2% fetal bovine serum and standard cell culture hormones (Dexamethasone 1 1 mM; mM, T3 1 μM, ITS 100X Gibco cat # 41400045) on 6-well ...
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bioRxiv - Neuroscience 2021Quote: ... 2°: goat anti-mouse AF488 (1:500) (Invitrogen, A-11001), and donkey anti-rabbit Cy5 (1:200 ...
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bioRxiv - Neuroscience 2021Quote: ... and ethidium homodimer-1 (2 mM in DMSO, L3224, ThermoFisher) were added directly to the cell medium to a final concentration of 1 μM each ...
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bioRxiv - Neuroscience 2021Quote: ... stained with Neurotrace for 2 hours (1:500, N21479; Invitrogen), rinsed twice with 2x SSCT and mounted on a slide with Fluoromount-G ...
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bioRxiv - Microbiology 2019Quote: ... N-2 supplement (1:200; catalog number 17502-048; Invitrogen), 20 ng/ml fibroblast growth factor (catalog number 4114-TC-01M ...
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bioRxiv - Molecular Biology 2020Quote: ... and HA tag (2-2.2.14; ThermoFisher Scientific 26183, 1:10,000), used with goat anti-mouse IgG AlexaFluor®568 (ThermoFisher Scientific A-11004 ...
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bioRxiv - Microbiology 2019Quote: ... supplemented with 1% penicillin-streptomycin with 2 mM glutamine (Invitrogen), 2% B27 (Invitrogen) ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were loaded with 1 μM fura-2 AM (Invitrogen) for 40 min before the measurements at 37°C ...
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bioRxiv - Bioengineering 2021Quote: ... 1 ng/mL human FGF-2 (ThermoFisher #68-8785-63), and 1% Primocin (Invivogen #ant-pm-1 ...
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bioRxiv - Neuroscience 2020Quote: ... 1% L-glutamine and 2% penicillin/streptomycin (Invitrogen, Paisley, UK). All cells were incubated at 5% CO2 in a humidity-controlled environment (37°C ...