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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for TIM 3 Human HEK 293 Fc His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The following antibodies were used: mouse anti-His (MA1-21315, Thermo Scientific), goat anti-mouse HRP (405306 ...
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bioRxiv - Neuroscience 2022Quote: ... and desmin were inserted into expression vectors pcDNA6 V5-His A (Invitrogen) using BamHI/XhoI ...
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bioRxiv - Microbiology 2022Quote: ... which is RPMI-1640 media containing 10% heat-inactivated (HI)-FBS (Gibco) and 1x penicillin-streptomycin-glutamine (Gibco) ...
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bioRxiv - Molecular Biology 2022Quote: ... were run with 1X Hi-Density TBE Sample Buffer (Thermo Fisher # LC6678) and 1X TBE Running Buffer ...
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bioRxiv - Immunology 2023Quote: His-tagged proteins were purified with HisPurTM Ni-NTA resin (Thermo Fisher). Briefly ...
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bioRxiv - Biochemistry 2023Quote: TOPOVIBL452-579 was cloned as a His-tag fusion into pProEXHTb (Invitrogen). REC114-MEI4 complex and TOPOVIBL452-579 were individually expressed in E ...
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bioRxiv - Immunology 2022Quote: ... Avi/His-tagged proteins were purified from transiently-transfected Expi293F cells (Gibco) by nickel affinity chromatography and SEC (Barnes et al. ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher), and rabbit polyclonal anti-mCherry (ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... His-VASP was bound to HisPur™ Ni-NTA Resin (ThermoFisher #88221), washed twice with 5 CV of wash buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... and incubated with 6x His-tag monoclonal primary antibody (MA1-135; ThermoFisher) overnight at a dilution of 1:750 ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibodies used in this study were rabbit polyclonal anti-His (ThermoFisher) and mouse monoclonal anti-FLAG (Sigma) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10-15 mL of His-Pur Ni-NTA resin (Thermo Fisher Scientific) was used per L of cell culture ...
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bioRxiv - Immunology 2023Quote: ... and coated with 1 mg of magnetic beads (Dynabeads His-Tag, Invitrogen) as previously described21 ...
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bioRxiv - Biophysics 2024Quote: ... with 2.5 μl Novex™ Hi-Density TBE Sample Buffer (5X) (Invitrogen). The gel was run in 0.5x TBE buffer at 120 V for 42 min and stained with 1x SYBR™ Gold Nucleic Acid Gel Stain (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS; Gibco, USA) and 100 U mL-1 penicillin–streptomycin (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 10 % heat inactivated Fetal Bovine Serum (HI FBS) (10500064; Gibco) and 1x antibiotic-antimycotic drug (100 units/mL of penicillin ...
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bioRxiv - Microbiology 2023Quote: ... plates were coated with 2 µg/ml anti-His (Invitrogen #MA1-21315) over night and blocked for 1hour as described above ...
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bioRxiv - Biochemistry 2024Quote: ... His-tag purification using Ni-NTA resin (Thermo Fisher, Massachusetts, United States) was performed and rubisco was eluted by SUMO tag cleavage with bdSUMO protease (as produced in Davidi et al ...
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bioRxiv - Biophysics 2021Quote: ... at a ratio of 1:1.5:1.75:2 (FAM155A-3×FLAG:NALCN-1077-3×HA-GFP:UNC79-3×FLAG:UNC80-3×FLAG) using Lipofectamine 3000 (Thermo Fisher Scientific) and incubated for 40-48 hours ...
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bioRxiv - Synthetic Biology 2023Quote: ... and 10 μL human IgG (Human IgG Isotype Control, ThermoFisher Scientific #02-7102 ...
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bioRxiv - Pathology 2019Quote: Mouse podocytes generated from wild-type and INF2 KO C57BL/6 mice (Supplementary Figure 3) and human podocytes (43) were maintained in RPMI 1640 (ThermoFisher Scientific, Waltham MA) supplemented with 10% fetal bovine serum (ThermoFisher) ...
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bioRxiv - Neuroscience 2022Quote: ... HLA-DR Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:50 Clone: LN-3, Invitrogen, Thermo Fisher Scientific, Waltham, MA, USA), TMEM119 (Rabbit Anti-Human ...
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bioRxiv - Neuroscience 2021Quote: ... Hs05441121_cn (human) (ThermoFisher). This line has been submitted to the European Mouse Mutant Archive (EMMA ...
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bioRxiv - Neuroscience 2021Quote: ... Hs06560655 (human) (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Human fibronectin (Invitrogen) was added to stamps at a concentration of 40 µg/ml for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK 293T/17 (ATCC CRL-11268) cells were plated and co-transfected with Lipofectamine 3000 (L3000015 Invitrogen), 3rd generation packaging vectors pRSV-REV #12253 ...
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bioRxiv - Cell Biology 2020Quote: ... 1.3×105 HEK-293T cells per cm2 were reverse transfected with Lipofectamine® 2000 (Thermo Fisher Scientific) following manufacturer’s instructions using 200 ng psiCHECK™ 2 (Promega ...
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bioRxiv - Biophysics 2021Quote: A commercial HEK cell suspension cell line optimized for protein expression Expi293-F (Cat. No. A14635, Gibco Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... HEK-293E/HeLa cells were transfected with indicated constructs 2 days before experiments using lipofectamine2000 (Thermo Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: HEK-293T cells (ATCC CRL-3216) are maintained in Dulbecco’s modified eagle medium (DMEM, Thermo Fisher 10569044) supplemented with 10% fetal bovine serum (Thermo Fisher 26140079 ...
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bioRxiv - Molecular Biology 2019Quote: Jurkat T cells and HEK 293T (CRL-3216, ATCC) were maintained in RPMI (21875-034, Life Technologies) supplemented with 10% FCS (Life Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK-293T cells were transfected with the anti-Chi3l1 construct using Lipofectamine™ 3000 (Invitrogen, L3000015, CA). Supernatant was collected for 7 days and the antibody was purified using a protein A column (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2020Quote: HeLa and HEK 293T cell lines were cultured in Dulbecco’s modified Eagle medium (DMEM; Thermo Fisher Scientific) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2024Quote: ... while HEK-293T and Vero E6 cells were cultured in DMEM medium (Dulbecco’s Modified Eagle Medium; Gibco). Both media were supplemented with 10% (v/v ...
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bioRxiv - Cell Biology 2024Quote: HEK cells and zebrafish samples were tagged with TMT10plex™ Isobaric Label Reagent Set (Thermo Scientific, #90111), and fibroblast cells with TMTpro™16plex Isobaric Labels (Thermo Scientific ...
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bioRxiv - Molecular Biology 2024Quote: All DNA plasmids were transfected into Hek-Luc cells using Lipofectamine™ LTX Reagent (Thermo Fisher Scientific). Cells were seeded 24 hours prior to transfection to ensure high viability and suitable confluency ...
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bioRxiv - Bioengineering 2023Quote: HEK-293T or Neuro 2A cells were cultured on 12-mm cover glass (Fisher Scientific, Pittsburgh, PA) coated with fibronectin (Sigma Aldrich ...
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bioRxiv - Genetics 2023Quote: ... HEK-293FT cells were cultured in DMEM medium supplemented with GlutaMAX-I (Thermo Fisher Scientific, 10569-010), 1× MEM Non-Essential Amino Acids (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... required pLL5.0 expression vectors and packaging vectors were transfected into HEK-293T cells using Lipofectamine 2000 (Invitrogen). 24 hrs after transfection cell medium was changed to fresh DMEM supplemented with 10% serum ...
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bioRxiv - Neuroscience 2023Quote: ... HEK 293T cells were transiently transfected with cDNA constructs encoding proteins of interest using Lipofectamine 2000 (Invitrogen) 16-24 hours before the start of the experiment ...
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bioRxiv - Molecular Biology 2023Quote: MCF-7 and HEK-293T cells were obtained from ATCC and maintained in high glucose DMEM (Invitrogen) at 37℃ with 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... HEK-293T cells were transfected with the bivalent FRGxCTLA-4 construct using Lipofectamine™ (Invitrogen, CA, USA). The bispecific antibody was purified from the collected supernatant using a protein A column (Thermo Scientific # 89960 ...
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bioRxiv - Immunology 2024Quote: ... CyRPA protein were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) following the manufacturer’s protocol using ExpiFectamine™ (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2024Quote: ... HEK 293T cells were transiently transfected with indicated constructs using the lipo2000 (1 mg/ml, LIFE technologies) reagents ...
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bioRxiv - Neuroscience 2024Quote: HEK-293T or Neuro 2A cells were cultured on 12-mm cover glass (Fisher Scientific, Pittsburgh, PA) coated with fibronectin (Sigma Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Suspension culture of HEK-293T cells was cultivated in the 1:1 mixture of FreeStyle F17 (Gibco) and EX-CELL 293 media ...
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS, ThermoFisher Scientific), L-glutamine (ThermoFisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... with 20% heat deactivated FCS (Hyclone) and 0.22% sodium bicarbonate (Gibco) at 37°C with 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... with 10% (v/v) fetal calf serum (FCS; Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... HMI-11 containing 10% (v/v) fetal calf serum (FCS; Gibco). ATPase γ genotypes were verified as detailed in Dewar et al. ...