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Citations for Thermo Fisher :
2601 - 2650 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The PCR products obtained by SGA were cloned into the commercial directional vector pcDNA3.1D/V5-His-TOPO according to the manufacturer’s instructions (Invitrogen). The correct pcDNA3.1-Env plasmid used to produce wild type pdsudoviruses were selected by sequencing ...
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Neutralizing activity of broadly neutralizing anti-HIV-1 antibodies against primary African isolatesbioRxiv - Immunology 2020Quote: ... The cytomegalovirus (CMV) promoter was amplified by PCR from the pcDNA 3.1D/V5-His-TOPO plasmid (Life Technologies) with forward primer 5’-AGTAATCAATTACGGGGTCATTAGTTCAT-3’ and reverse primer 5’ CATAGGAGATGCCTAAGCCGGTGGAGCTCTGCTTATATAGACCTC-3’ ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies (ACE2, dilution 1:100; TMPRSS2, 1:500; CD147, 1:500; 6x-HIS-tag (Invitrogen MA1-21315), 1:1000 ...
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bioRxiv - Genetics 2020Quote: ... Purified N-HIS-FAM19A5 was then digested overnight at 30°C with AcTEV protease (Thermo Fisher Scientific, USA) to remove the His-tag ...
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bioRxiv - Immunology 2022Quote: ... Wildtype and mutant coding sequences were transferred to the pEF1/V5-His vector (cat. no. V920; Life Technologies) for overexpression ...
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bioRxiv - Synthetic Biology 2022Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The washed membrane was then incubated overnight at 5°C with a 6x-His-Tag monoclonal antibody (Invitrogen) diluted 2500-fold in PBS-Tween ...
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bioRxiv - Molecular Biology 2024Quote: The cDNA sequence of human Sph1 was subcloned from the pcDNA3.1/V5-His-TOPO expression vector (Invitrogen, USA) 62 into the Venus-BiFC plasmids previously described 37 ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged: The lysate was mixed head-over-head with Ni-NTA agarose resin (Thermo Scientific™, USA) for 60 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: LZTR1WT and LZTR1L580P were expressed as C-terminal His-tagged proteins in Expi-293F cells (Thermo Fisher Scientific). pcDNA3.1-LZTR1-Myc-6xHis plasmid was a gift from Jens Kroll (Heidelberg University and German Cancer Research Center (DKFZ-ZMBH Alliance)).56 The LZTR1L580Pvariant was introduced into the plasmid by site-directed mutagenesis as previously described.11 Cells were transfected using ExpiFectamine 293 Reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... We diluted anti-stem Abs (starting at the lowest concentration, which yielded measurable HI) in C1q-DPBS (Gibco) (220 nM) ...
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bioRxiv - Developmental Biology 2023Quote: ... Reverse transcription for other transcripts was performed using the hi-capacity cDNA reverse transcription kit (Applied Biosystems #4368814) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: His-CLASP1 protein was expressed in baculovirus-infected Sf9 insect cells using the Bac-to-Bac system (Invitrogen). After the first amplification ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were transfected with a pMT Puro His-PC-SNAP-Elp3 vector using ExpiFectamine™ Sf(A389l5, Gibco) using manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... The beads were then incubated with a 1:1000 dilution of chicken anti-His antibody (Invitrogen, PA1-9531) on ice for 20 min ...
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bioRxiv - Cell Biology 2023Quote: ... entry clones containing individual TSWV proteins were recombined with a pIB/V5-His destination vector (Thermo Fisher Scientific) containing either GFP or mRFP in both amino and carboxy terminal fusions when possible ...
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bioRxiv - Microbiology 2023Quote: ... TCID50) were calculated by IFA using HIS α NDV and Alexa FluorTM 488 α-rabbit secondary antibody (Invitrogen) in two independent experiments.
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bioRxiv - Microbiology 2024Quote: ... we followed the vendor supplied protocol of the His-Pur Ni-NTA Purification Kit (3 ml, Thermo Fisher). The flow through ...
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bioRxiv - Developmental Biology 2024Quote: Drosophila S2 cells were transfected with the indicated plasmids or blank pMT-V5-His vector (Invitrogen cat#V412020) using Effectene transfection reagent (Qiagen cat#301427) ...
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bioRxiv - Plant Biology 2024Quote: Cloning and expression of His-mVenus-SUF4 was based on the Bac-to-Bac Baculovirus Expression system (ThermoFisher). The control pFastBac-mVenus and pFastBac-mVenus-SUF4 isoform 1 plasmids were obtained from Dr ...
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bioRxiv - Genetics 2024Quote: ... and hybridizing the Hi-C libraries with custom probes before enrichment via Dynabeads MyOne Streptavidin Beads T1 (ThermoFisher). After library enrichment ...
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bioRxiv - Microbiology 2024Quote: The HylB expression vector pAOJ66 was generated by amplifying the ef0818 gene with primers AOJ_651 and AOJ_652 and cloning into NcoI-HF/ApaI digested pBAD/myc his A (Invitrogen). The vector was propagated in E ...
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bioRxiv - Immunology 2024Quote: ... Blots were blocked for 30 min to 1 h in 5% milk in TBST (1X Tris-buffered saline with 0.1% Tween 20) and then incubated with an anti-6X-His tag primary antibody (1:1,000 dilution, Invitrogen) (overnight ...
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bioRxiv - Cell Biology 2019Quote: ... FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) was purchased from Invitrogen. Rapamycin was purchased from LC Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... lysates were spun at 10,000 x g for 10 minutes at 4 °C prior to reaction with 4- acetamido-4’-maleimidyl-stilbene-2,2’-disulfonic acid (AMS) (ThermoFisher Scientific). AMS alkylation was performed by vortexing the lysates in 15 mM AMS ...
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bioRxiv - Neuroscience 2023Quote: Cultured neurons were incubated for 1 min with fluorescent dye N-(3-triethylammonium-propyl)-4-(6-(4-diethylamino)phenyl)-hexatrienyl)pyridinium dibromide (FM4-64; 4 µM; Invitrogen) and loaded by stimulation (10 Hz ...
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bioRxiv - Microbiology 2021Quote: ... The LB medium contained 4 μg/mL FM 4-64 stain (ThermoFisher) to stain all cells ...
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bioRxiv - Biophysics 2022Quote: ... sorbitol (4% w/v) and glycerol (4% v/v) (Fisher Scientific, Mumbai). Growth rates were measured by monitoring optical density at 600 nm (OD600 ...
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bioRxiv - Cell Biology 2019Quote: ... 4 mM Glutamax (Gibco), as well as L-serine and glycine in varying concentrations ...
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bioRxiv - Immunology 2021Quote: ... and 4% FBS (Gibco). Plates were incubated 2 days at 37°C ...
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bioRxiv - Biochemistry 2019Quote: ... Fluo-4 Direct (Invitrogen) was added to the cells at 1X dilution according to manufacturers’ protocol and visualised using a LS620 Etaluma microscope at the same time point as CLIC1 assay cells were fixed ...
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bioRxiv - Cancer Biology 2020Quote: ... 4 μl pyrophosphatase (Thermofisher), 2 μl Phi29 Polymerase (Thermofisher) ...
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bioRxiv - Neuroscience 2021Quote: ... Alexa 594 (4%, Invitrogen) and neurobiotin (0.05% ...
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bioRxiv - Neuroscience 2022Quote: ... 4 mM Glutamax (Invitrogen), penicillin (100 U/mL) ...
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bioRxiv - Bioengineering 2022Quote: ... Claudin-4 (Invitrogen; 329494), TRPV6 (Proteintech;13411-1-AP) ...
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bioRxiv - Microbiology 2021Quote: ... 4 nM NTPs (ThermoFisher) and 50U Superase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... at 4°C (Nunc MaxiSorp flat bottom ...
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bioRxiv - Cancer Biology 2021Quote: ... 4% in PBS (Affymetrix) was used to fix cells ...
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bioRxiv - Cell Biology 2021Quote: ... 4 mM glutamine (Gibco), 100 units/ml of penicillin ...
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bioRxiv - Microbiology 2022Quote: ... 4-chlorobenzenesulfonate (DiD, Invitrogen), as previously described [10] ...
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bioRxiv - Neuroscience 2022Quote: ... 4 mM Glutamax (Invitrogen), penicillin (100 U/mL) ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-20% (ThermoFisher; XP04205BOX) under reducing and denaturing conditions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 4-20% (ThermoFisher; XP04205BOX) under reducing and denaturing conditions ...
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bioRxiv - Microbiology 2023Quote: ... 4% EGF (ThermoFisher Scientific), and 50 ng/mL KSR (Gibco)] ...
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bioRxiv - Developmental Biology 2023Quote: ... 4% BSA (Gibco, # 15260), 1% L-Glutamine (Gibco ...
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bioRxiv - Immunology 2023Quote: ... 4% PFA (Thermo Fisher) was added into each spleen section and incubated at RT for 20 mins to fix the tissue ...
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bioRxiv - Biophysics 2023Quote: ... 4 mM glutamine (Gibco), 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... 4 mM Glucose (Gibco) and 50 ng/mL IGF-1 (Sigma) ...