Labshake search
Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: ... IL-6 serum levels were measured using an ELISA kit (88-7064, Invitrogen). The assay was performed following the manufactureŕs instructions and guidelines ...
-
bioRxiv - Immunology 2024Quote: ... and IL-17A BV711 (#407-7177-82, Thermo Fisher Scientific, Houston, TX, USA). Cells were assessed using a 4-laser Attune NxT flow cytometer (Thermo Fisher Scientific ...
-
bioRxiv - Genomics 2024Quote: ... The blots were washed and incubated with secondary antibodies (Thermo Scientific, IL, USA). The protein bands were detected by the standard chemiluminescence method (Thermo Pierce Femto ...
-
bioRxiv - Cell Biology 2019Quote: ... stable expression was maintained using 0.9 mg/ml Geneticin (G418) added to the standard HEK293 growth medium (MEM, supplemented with 10% FBS, both from Gibco, Life Technologies, Darmstadt, Germany).
-
bioRxiv - Cell Biology 2021Quote: HEK293T (ATCC® CRL-3216™) and S1/S2 DKO HEK293 cells were maintained in cell culture in 1X DMEM (41965-039, Thermo Fisher Scientific) supplemented with 10% FBS ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... An inducible D540K hERG HEK293 cell line (T-REx D540K hERG HEK) was generated using the Flp-In T-REx system (Thermo Fisher Scientific, MA, USA). The D540K hERG mutant was made site-directed mutagenesis from the wild-type (WT ...
-
bioRxiv - Immunology 2023Quote: Human-derived cell lines used in this study were initially obtained from accredited commercial suppliers: (1) HEK293 (human embryonic kidney cell line, FreeStyle® 293-F) was obtained from Invitrogen (San Diego, CA) for use as a recombinant protein expression vehicle ...
-
bioRxiv - Neuroscience 2023Quote: ... HEK293 cells were first prepared for transfection by plating into 12-well tissue culture plates (Nunc 12-565-321; Thermo Fisher Scientific, Waltham, MA) at a density of ∼0.5-2 x105 cells per well and grown to ∼80-90% confluence with standard media ...
-
bioRxiv - Immunology 2023Quote: RAVV GPΔMuc fused with Strep II and Avi tags was expressed in HEK293S GNTI−/− cells (ATCC, Manassas, VA) using 293fectin transfection reagent and FreeStyle Media (Thermo Fisher Scientific, Waltham, MA). Supernatants were purified using Streptactin XT Resin purification (IBA Lifesciences ...
-
bioRxiv - Neuroscience 2024Quote: ... Recombinant single-strand AAV vectors were produced using the ultracentrifugation method described in a previous paper were produced using HEK293-T cells (HCL4517; Thermo Fisher Scientific; Waltham, MA), as described previously26 ...
-
bioRxiv - Cell Biology 2019Quote: ... First antibody incubation was done O/N at 4°C and the secondary HRP-coupled antibodies goat anti-Mouse IgG (H+L) (ThermoFisher scientific; 31430; 1:10,000) or polyclonal HRP-conjugated goat-anti-rabbit IgG (ThermoFisher scientific ...
-
bioRxiv - Genetics 2021Quote: ... and incubated overnight at 4°C with mouse anti-H4K3me3 (Wako #305-34819 at 1:500 dilution in PBST) or rabbit anti-H3K4me2 (Invitrogen #710796 at 1:20,000) primary antibodies ...
-
bioRxiv - Molecular Biology 2019Quote: ... in PBS at 4°C for overnight and then further incubated with Alexa Fluor 647-conjugated anti-mouse IgG (Thermo Fisher Scientific, 1:1000) in PBS at 4°C for overnight with Hoechst 33342 for nuclear staining ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were then incubated with horseradish peroxidase (HRP)-conjugated secondary antibodies (1:1000, chicken-anti mouse IgG Invitrogen Cat# A15975 or chicken-anti rabbit IgG Invitrogen Cat# A15987, Table 4) in cell culture medium containing 10% FBS for 1 h at RT ...
-
bioRxiv - Neuroscience 2020Quote: ... Cells were then incubated overnight at 4°C in blocking solution (PBS, 2% normal goat serum, 3% BSA) and primary antibodies (mouse anti-HA, Invitrogen; rabbit anti-GFP, Invitrogen). Cells were then immunolabeled with Alexa-conjugated secondary antibodies (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2021Quote: ... permeabilized with 0.1% Triton X-100 in PBS for 10 min and blocked with 2% BSA solution in PBS for 1 h followed by overnight incubation at 4°C with primary antibodies diluted in 2% BSA blocking solution: mouse monoclonal [1F7G5] anti-CKS2 (1:100 dilution; Thermo Fisher Scientific; 37-0300), rabbit polyclonal anti-P63 (p63α ...
-
bioRxiv - Bioengineering 2022Quote: ... Devices were washed with PBS for 4-6 hrs and subsequently incubated with Alexa Fluor 647-conjugated goat anti-mouse IgG (H+L) (ThermoFisher cat# A32728, 1:300) and Hoechst 33342 (Sigma-Aldrich cat# B2261 ...
-
bioRxiv - Genetics 2020Quote: ... The primary antibodies were used for overnight shaking at 4°C by the following dilution: mouse anti-V5 (Invitrogen Cat# R960-25 RRID: AB_2556564), 1:2,000 ...
-
bioRxiv - Neuroscience 2022Quote: Sections were washed twice in 0.01 M PBS and incubated overnight at 4 °C with appropriate Alexa-labelled secondary antibody (goat anti-mouse Alexa plus 555-labelled secondary antibody; Invitrogen; 1:1000, Cat# A32727) in PBS with 10 % NGS ...
-
bioRxiv - Developmental Biology 2022Quote: ... discs were fixed in 4% (w/v) paraformaldehyde/PBS for ∼45 min and goat anti-mouse Pacific Blue antibody (Life Technologies, 1:200 dilution) was used as the secondary antibody.
-
bioRxiv - Cell Biology 2024Quote: ... Sections were washed three times with 1X PBS followed by incubation with IgG-conjugated secondary antibodies (goat anti-mouse IgG overnight and Alexa Fluor™ 488 goat anti-rabbit IgG for 4 h (both from ThermoFisher Scientific, 1:1000). Sections were washed three times as above and followed by 1 h incubation with Alexa Fluor™594 Tyramide SuperBoost for ERG staining ...
-
bioRxiv - Cell Biology 2022Quote: ... 1:200 O/N at 4 °C) and Alexa Fluor 594-conjugated anti-mouse IgG antibody (#A11005, Invitrogen, 1:2000 for 2 hours RT). Nuclei staining was done in 0.5 μg/mL Hoechst 33324 (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... 1:200) at 4 °C 48 hours and in secondary antibodies simultaneously (Goat Anti-mouse 1:200, Invitrogen, Goat Anti-Rabbit 1:200, Jackson) at RT for 1 hour ...
-
bioRxiv - Systems Biology 2023Quote: ... 1 vein graft slide containing 2-4 cross sections was double stained with the general macrophage marker Mac-3 (rat anti-mouse, Fisher Scientific, B550292, 1:600) and either iNOS (rabbit anti-mouse ...
-
bioRxiv - Microbiology 2023Quote: ... Lysates were then incubated for 3 h at 4 °C with 50 mL of Pierce Protein-G beads bound with mouse anti-Ty antibody (Invitrogen Ty1 Tag Monoclonal BB2). Beads were washed 6 times with wash buffer (200 mM Tris pH 9 ...
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated in dark at room temperature for 4 hours in AlexaFluor-donkey anti-Rabbit 594 and donkey anti-Mouse 488 (Thermo Fisher Scientific; 1:500). Sections were mounted and cover slipped (Fluoromount ...
-
bioRxiv - Immunology 2024Quote: ... differentiated Th2 cells were restimulated with anti-mouse CD3 (4 µg/mL) for 3 hours followed by 2 hours of incubation with monensin (Thermo Fisher Scientific, MA, USA) at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: All cell lines were cultured in DMEM media supplemented with sodium pyruvate and 10% Hi-FBS (Life Technologies). To express protein constructs in living cells ...
-
bioRxiv - Genetics 2020Quote: ... Germany) 1 μl of each PCR product was edited with Hi-Di Formamide (20 μl, ThermoFisher, Massachusetts, USA) and Gene Scan 500 ROX dye Size Standard (0.5 μl ...
-
bioRxiv - Molecular Biology 2020Quote: ... 5% CO2 for 24 h before transient transfection with plasmids encoding Affimer-His constructs using Lipofectamine 2000 (ThermoFisher), as per the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... HEL and K562 cells were all from American Type Culture Collection (ATCC) and cultured in RPMI with 10% HI FBS except for K562 (grown in IMDM (Gibco, #21980-032) with 10% HI FBS ...
-
bioRxiv - Molecular Biology 2019Quote: Wild-type and indicated mutants of MmEsco1 were cloned into pEF6/Myc-His B vector (Thermo Fisher Scientific) containing C-terminal myc and His tags ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Anti-His immunoblots were performed using 1:2,500 diluted rabbit Anti-6xHis antibody (PA1-983B, Thermo Fisher Scientific) in 5% w/v milk in TBST for 1 h ...
-
bioRxiv - Biochemistry 2020Quote: Both untagged and His-tagged EncFtn proteins were quantified using the Pierce BCA Assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions for the Test-tube procedure ...
-
bioRxiv - Biochemistry 2020Quote: ... Uncleaved His-tagged proteins were removed by incubation of the sample with 1 ml HisPur™ (Thermo Fisher) nickel resin for 1h at 4°C ...
-
bioRxiv - Cell Biology 2021Quote: ... rKLK10 with a 6x C-terminal His tag was affinity purified using HisPur Ni-NTA Resin (Thermo Scientific) per the manufacturer’s instruction (Figure S16 ...
-
bioRxiv - Molecular Biology 2020Quote: ... RNA-protein complexes were purified with Pierce(tm) His Protein Interaction Pull-Down Kit (Thermo Fisher Scientific, #21277) with the following modifications ...
-
bioRxiv - Biochemistry 2021Quote: ... The resulting cyclic peptide-mRNA:cDNA library was counter-selected 3 times against His Pull-Down Dynabeads (Thermo Fisher), and affinity selected against 200 nM bead-immobilised Retromer for 30 min ...
-
bioRxiv - Biophysics 2021Quote: Detergent extracted His-MBP-NavSp1 was applied to a 40 mL POROS MC 20 Ni2+ column (Applied Biosystems) equilibrated with buffer A (200 mM NaCl ...
-
bioRxiv - Cell Biology 2022Quote: ... Sequencing runs were conducted on Proton sequencer using the PI™ Hi-Q™ Sequencing 200 Kit (Thermofisher) following the recommendations of the manufacturer ...
-
bioRxiv - Biochemistry 2019Quote: ... Each protein was first purified by metal affinity chromatography using 2ml of His purTM cobalt column (Thermo Scientific). The tag was removed by cleavage with TEV protease followed by purification on a second cobalt affinity chromatography ...
-
bioRxiv - Molecular Biology 2019Quote: ... and cDNA was immediately synthetized using Hi-capacity RNA-to-DNA™ kit (Applied Biosystems™, ref. 4388950) both according to manufacturers instructions ...
-
bioRxiv - Microbiology 2020Quote: ... and sequenced using the Ion PGM Hi-Q View sequencing kit (Life Technologies, Grand Island, NY, United States) with the Ion PGM™ System technology ...
-
bioRxiv - Cell Biology 2019Quote: ... Antibodies used for the IP western blots are as follows: anti-His monoclonal (1:3000 dilution; ThermoFisher Scientific) and goat anti-mouse HRP (1:15,000 dilution ...
-
bioRxiv - Molecular Biology 2022Quote: ... and sub cloned in BamHI and SalI site of pCDNA3.1/myc-His(A) mammalian expression vector (Invitrogen, USA) to generate pCDNA3.1-PAX9-myc-His(A ...
-
bioRxiv - Biochemistry 2021Quote: ... the array was washed and probed with an anti-His primary antibody (Thermo Fisher, MA121315, 1:1000 dilution), after which anti-mouse HRP-conjugated secondary antibody (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2020Quote: ... were coated at room temperature for 8 hours with 1ug/mL PolyRab anti-His antibody (ThermoFisher, PA1-983B), followed by overnight blocking with blocking buffer containing 1x PBS ...
-
bioRxiv - Immunology 2021Quote: ... PCR was carried out with gene-specific primers (Table 1) using Phusion Hi-Fidelity PCR Mastermix (Thermo Scientific).