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Citations for Thermo Fisher :
2801 - 2850 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: HCMV US18 and US20 were amplified from the HCMV TB40/E BAC (accession #EF999921) and subcloned into pcDNA3.1-V5/His (Invitrogen) via the KpnI/NotI sites to generate pcDNA3.1 US18-V5/His and pcDNA3.1 US20-V5/His ...
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bioRxiv - Molecular Biology 2023Quote: ... ceSMSγ and ceSMSr cDNAs were PCR amplified and ligated into the copper-inducible pMT/V5-His B vector (Invitrogen).
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bioRxiv - Genetics 2023Quote: ... standard fragment analysis conditions) with 0.8 ul PCR product is loaded in 9.4 ul Hi-Di Formamide (Applied Biosystems), with 0.1 ul GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... and gB[H527P]-His EVs was performed at 300 keV on a Titan Krios electron microscope (Thermo Fisher scientific) equipped with a Gatan K3 (5 μm/pixel ...
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bioRxiv - Microbiology 2023Quote: ... we initially digested the pNL4-3 plasmid with EcoRI and XhoI and subcloned this fragment into pcDNA3.1/myc-His A (Invitrogen). To generate pcDNA3.1 NL4-3 Env TMTR ...
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bioRxiv - Immunology 2023Quote: ... Amplification and cloning of the truncated versions of TGM4 (D1-3 and D4-5) was performed by PCR amplification using proofreading Taq polymerase Phusion Hi as per the manufacturer’s instructions (Invitrogen), full-length codon-optimised TGM4 as template ...
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bioRxiv - Biochemistry 2024Quote: ... followed by a 6× His-tag was cloned into the BamHI and XbaI sites of the pcDNA 3.1 vector (#V79020, Invitrogen) containing the vascular endothelial growth factor receptor 1 (VEGFR ...
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bioRxiv - Genetics 2024Quote: ... together with internal size standard ladder where 0.8 µl PCR product was loaded in 9.4 µl Hi-Di Formamide (Applied Biosystems), with 0.1 µl GeneScan 500 LIZ (Applied Biosystems) ...
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bioRxiv - Neuroscience 2023Quote: ... for 2 minutes followed by ice cold 4% PFA for 4 min with a flow rate of 6ml/min and post-fixed (overnight at 4°C) in 4% PFA (Thermo Fisher Scientific) in 1x PBS (pH 7.4) ...
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bioRxiv - Cancer Biology 2019Quote: ∼1 x 106 HEK293 cells grown at 70% confluency on 35 mm dish embedded with 12 mm glass viewing area (Nunc, Thermo Fisher Scientific Inc., Waltham, MA, USA) were transfected with appropriate plasmids using Lipofectamine 3000 ...
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bioRxiv - Pathology 2024Quote: ... HT7 was the only anti-mouse antibody used and a mouse-on-mouse blocking agent (Invitrogen: R37621) was applied for 30 minutes post protein block washes to reduce noise from secondary antibody binding and an additional three 5-minute washes were added before primary antibody incubation ...
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bioRxiv - Neuroscience 2023Quote: ... Sections stained with mouse primary antibodies were blocked in ReadyProbes “Mouse on Mouse” IgG blocking solution (Thermofisher) for 1 hour before the initial blocking step ...
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bioRxiv - Cell Biology 2019Quote: ... for 20 minutes followed by overnight incubation in primary antibodies at 4°C (mouse anti-GM-130, BD Transduction laboratories #610822 1:250; rat anti-VE-cad BV13, ThermoFisher Scientific #14-1441-82 1:500) diluted in blocking buffer ...
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bioRxiv - Biochemistry 2020Quote: ... AB_2617216) was added to sections overnight at 4°C followed by Alexa Fluor®-conjugated goat IgG against mouse IgM (Invitrogen, Carlsbad, CA, 1:500 dilution), for 40 minutes ...
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bioRxiv - Genetics 2024Quote: ... were incubated overnight at 4°C followed by the secondary antibodies (anti-rabbit 488, A11034 Invitrogen, 1:400; anti-mouse 555, A31570, Invitrogen, 1:400; DAPI, Roth, 1:400). Finally ...
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bioRxiv - Cell Biology 2023Quote: ... Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C: mouse anti-v5 (Thermo Fisher Scientific, Cat. # R96025; 1:2000), mouse anti-Flag M2 (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: ... the tissues were incubated at 4°C for 1 day with the following secondary antibodies as appropriate: Alexa 546-conjugated anti-mouse IgG (Thermo Fisher, A11030, 1:250–1000 dilution), Alexa 488-conjugated anti-mouse IgG (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... anti-mouse IgM-HRP and anti-mouse IgA-HRP (ThermoFisher), goat anti-mouse Igκ ...
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bioRxiv - Plant Biology 2024Quote: Mouse liver microsomes (purchased from Thermo: GIBCO Mouse (CD-1) Microsomes ...
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bioRxiv - Cancer Biology 2022Quote: ... Mm00440280_g1 (mouse, ThermoFisher), and TaqMan™ Universal Master Mix II ...
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bioRxiv - Genomics 2019Quote: ... α-mouse (Invitrogen #A31570), α-goat (Invitrogen #A21432) ...
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bioRxiv - Neuroscience 2021Quote: ... Mouse laminin (Gibco) was then added and incubated at 37°C for 4 hours ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: ... mouse (Affymetrix, 902931), performed by Cnkingbio Biotech Co. ...
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bioRxiv - Cell Biology 2021Quote: ... GST (Mouse, Invitrogen), GM130 (Mouse ...
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bioRxiv - Molecular Biology 2024Quote: ... mouse (Invitrogen, A315570), and rabbit (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... mouse (ThermoFisher, #23017015); Vitronectin ...
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bioRxiv - Developmental Biology 2019Quote: ... with successfully electroporated embryos fixed using 4% Paraformaldehyde (PFA; Fisher Scientific, 30525-89-4) at 4°C overnight.
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bioRxiv - Physiology 2019Quote: TT’s were visualized by staining with di-4-ANEPPS (4 µmol/l. Invitrogen, UK) as described in detail previously 8,32,70 ...
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bioRxiv - Cell Biology 2022Quote: ... with an Eagle 4 K x 4 K CCD camera system (Ceta CMOS, ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... and 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyltetrazolium Chloride (INT, #I00671G, Fisher Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... 4 µg protein was resolved on NuPAGE 4-12% Bis-Tris protein gels (ThermoFisher) and transferred to PVDF membrane (Fisher) ...
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bioRxiv - Neuroscience 2022Quote: ... containing 2 μM cell-permeant Fluo-4 acetoxymethyl ester (Fluo-4-AM, Thermo Fisher) with the addition of the equal quantity (1:1 v/v ...
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bioRxiv - Immunology 2022Quote: ... Hemocytes were fixed in 4% PFA (4% in PBS; J19943-K2, Thermo Fisher Scientific) for 30 min at RT ...
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bioRxiv - Physiology 2024Quote: ... Tim-4 (1:1000 dilution, rabbit anti-TIM-4 antibody, Invitrogen, PA5-116045, RRID:AB_2900679) and Lumican (1:1000 dilution ...
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bioRxiv - Neuroscience 2023Quote: ... followed by 4% formaldehyde (4% FA, 10% formalin [Thermofisher Scientific, #119690010] in 1x PBS), dissected out the spinal column ...
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bioRxiv - Cancer Biology 2021Quote: ... M-PER was supplemented with protease and phosphatase inhibitors (PPI; Thermo Scientific, Rockford, IL, USA). After aggressively vortexing these cells for 3x 1-minute increments ...
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bioRxiv - Cancer Biology 2021Quote: ... they were resuspended in mammalian protein extraction reagent (M-PER; Thermo Scientific, Rockford, IL, USA) supplemented with protease and phosphatase inhibitor (PPI ...
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bioRxiv - Developmental Biology 2021Quote: ... tissues were immersed in PBS with protease inhibitors (Pierce 88266, Fisher Scientific, Rockford, IL, USA) to minimize post-mortem degradation ...
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Extensive introgression among North American wild grapes (Vitis) fuels biotic and abiotic adaptationbioRxiv - Evolutionary Biology 2021Quote: ... DNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL, USA), DNA quantity with the DNA High Sensitivity kit on a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Brain sections were mounted on gelatin-coated glass slides (SuperFrost Plus, Thermo Fisher Scientific, IL) and stained for 20 min with a cresyl-violet solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined using the BCA protein assay kit (ThermoFisher Scientific, Rockford, IL, USA). 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad ...
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bioRxiv - Immunology 2021Quote: ... IL-2 Human Uncoated ELISA kit and IFNγ Human Uncoated ELISA kit (both from Invitrogen) and DuoSet Human IFN-gamma kit and Ancillary Reagent Kit 2 (R&D Systems ...
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bioRxiv - Biochemistry 2020Quote: WI-26 cells were plated in a Lab-Tek chamber slide (Nalgen Nunc International, IL) at 3 × 104 cells per well and incubated overnight ...
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bioRxiv - Neuroscience 2019Quote: ... The proteins were revealed using Pierce Silver Staining kit (Thermo Fisher Scientific, Rockford, IL, USA) according to manufacturer’s protocols ...
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bioRxiv - Bioengineering 2019Quote: ... and then incubated with 10 μg/cm2 MA(PEG)24 (Thermo Scientific, Rockford, IL, USA) for two hours ...
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bioRxiv - Molecular Biology 2019Quote: ... secondary antibodies were visualized with an enhanced chemiluminescence detection kit (Thermo Scientific, Rockford, IL, USA). The band intensities were quantified using Gene Tools software (Sygene ...
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bioRxiv - Microbiology 2021Quote: Cell culture supernatant was harvested and used to carry out ELISA for IL-1β (Invitrogen) and IL-18 (Invitrogen ...
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bioRxiv - Biochemistry 2020Quote: WI-26 cells were plated in a Lab-Tek chamber slide (Nalgen Nunc International, IL) at 3 × 104 cells per well and incubated overnight ...
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bioRxiv - Cancer Biology 2020Quote: ... One ng/mL recombinant human interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), was added to the media of the IL-6 dependent myeloma cell lines INA-6 ...
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bioRxiv - Physiology 2021Quote: ... and developed with SuperSignal® West Pico Chemiluminescent Substrate (Thermo Fisher Scientific, Rockford, IL, USA).