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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... 250 µl of the clarified lysate was mixed with 150 µl HIS-Tag isolation dynabeads (Invitrogen 10103D) and 750 µl US buffer for 2 h ...
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bioRxiv - Immunology 2022Quote: ... The His-tagged proteins were purified with the HisPur Ni-NTA Resin (Thermo Fisher Scientific cat.# 88221). After washing by wash buffer (25 mM Imidazole ...
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bioRxiv - Molecular Biology 2023Quote: Drosophila S2 cells were cultured at 23°C in Schneider’s media with 10% Gibco HI-FBS (Thermofisher) and 1% Gibco Antibiotic-Antimycotic (Thermofisher) ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant AtCSD1 protein containing a 6xHis-tag was purified using Dynabeads His-Tag (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... Eluted proteins were then incubated with Dynabeads His-Tag Isolation and Pulldown (Thermo Fisher Scientific, Waltham, Massachusetts) for 20 minutes and then washed 5 x 1 minute in His-tag Isolation Buffer (100 mM Na-phosphate ...
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bioRxiv - Molecular Biology 2022Quote: Recombinant Rep1ΔAD-His and its variants were incubated with 50 μl D–Galactose agarose beads (Thermo Scientific) overnight at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... and proteins were visualized using Ponceau stain and Western blot with monoclonal anti-His (Invitrogen MA1-21315), and polyclonal anti-GST (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The entire pENTR effector library was then transferred into the pIB/V5-His vector (Invitrogen; Table S4) using LR Clonase II (Invitrogen ...
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bioRxiv - Genetics 2023Quote: ... The Hi-C library is quantified using the Qubit DNA high sensitivity kit (Thermo Scientific, cat. Q32851) on a Qubit 2 fluorometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... and co-expressed with MEI41-127 that was cloned as a His-tag fusion into pProEXHTb (Invitrogen). Supernatants were applied to Strep-Tactin XT resin (IBA ...
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bioRxiv - Immunology 2023Quote: ... Filtered supernatants containing His-tagged proteins were passed slowly through HisPur Ni-NTA Resin (Thermo Fisher 88221) beads in columns ...
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bioRxiv - Developmental Biology 2023Quote: ... Su(H) coding sequence was cloned from GH10914 (DGRC Stock 5973) into pAc5.1/V5-His A (Invitrogen) using the following primers ...
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bioRxiv - Biochemistry 2023Quote: His-mATGL and its point mutants were recombinantly expressed in Expi293FTM cells (Thermo Fisher Scientific, Waltham, USA). The cells were cultivated in Expi Expression Medium (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... the crude DEL sample was loaded in Novex™ Hi-Density TBE Sample Buffer (5X, Invitrogen LC6678), and electrophoresis was run at 12 W constant power ...
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bioRxiv - Cancer Biology 2023Quote: ... His-tagged FH(44-511) was purified from the lysate using HisPurTM Cobalt Spin Columns (Thermo Fisher). Columns were washed using with buffer A (50 mM sodium phosphate pH 7.4 ...
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bioRxiv - Immunology 2023Quote: ... using an Ion PI Hi-Q Sequencing 200 kit and Ion PI Chip v3 (Thermo Fisher Scientific). The FASTQ files were generated using AmpliSeqRNA plug-in v5.2.0.3 in the Torrent Suite software (v5.2.2 ...
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bioRxiv - Synthetic Biology 2023Quote: All RBD antigens were His-tagged and purified using HisPur Ni-NTA resin (Thermo Fisher Scientific, 88222). Cell supernatants were diluted with 1/3 volume of wash buffer (20 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ... Cells were grown in Ham’s F-12K medium (ATCC) with HI-FBS and 1x Antibiotic-Antimycotic (Gibco) at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... Rubrum CbbM coding region in a His-bdSUMO plasmid acquired from Addgene65 using OneShotTM Top10 cells (Invitrogen). The constructs were expressed in OneShotTMBL21-AITM (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... with Radiant Probe Hi-ROX qPCR Kit (Alkali Scientific) and Taqman Gene Expression Assays (Thermo Fisher Scientific). The expression of target genes was normalized to Gapdh expression.
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant SdeC and its derivatives were bound to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1 μg enzyme per 25μl of packed resin in 1X ART buffer (20mM Tris 10mM NaCl ...
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bioRxiv - Microbiology 2024Quote: His-tagged recombinant full-length SdeC derivatives were adsorbed to Ni-NTA (Thermo Fisher Scientific, Cat# 88221) resin at 1μg enzyme per 25 μl of packed resin in 1X ART buffer for 1 hr at 4°C with end-over-end rotation ...
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bioRxiv - Bioengineering 2022Quote: ... 4 μl of 4 mM resazurin sodium salt (Acros Organics, Belgium), 0.002 U purified Castellaniella defragrans geraniol dehydrogenase ...
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bioRxiv - Biophysics 2019Quote: Isolated islets were loaded with 4 µM Fluo-4 AM (Invitrogen) for 45min at 37°C in imaging medium (125mM NaCl ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... isolated with Protein A Dynabeads (Invitrogen; 4 h at 4°C), washed thrice with RSB+T ...
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bioRxiv - Physiology 2020Quote: ... muscles were stained for 3 minutes with 10μM 4-(4-diethylaminostyrl)-N-methylpyridinium iodide (4-Di-2ASP, Molecular Probes) to allow imaging muscle with an upright epifluorescence microscope (Leica DMR ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were blocked for 20-30 minutes in SuperBlock solution (ThermoFisher, Rockland, IL) at room temperature before overnight incubation at 4°C in 3% skim/tris buffered saline (TBS ...
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bioRxiv - Immunology 2021Quote: ... Anti-IL-2 Ab conjugated to PE (clone JES6-5H4) manufactured by Invitrogen, purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... Protein concentration was determined using BCA protein assay kit (Thermo Scientific, Rockford, IL).
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bioRxiv - Physiology 2022Quote: ... Protein concentration was determined using the BCA assay (Thermo Scientific, Rockford, IL, USA). Mitochondrial respiration of isolated mitochondria was measured within two hours of isolation.
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bioRxiv - Immunology 2022Quote: ... RNA purity and quantity were evaluated with Nanodrop spectrophotometer (Thermo Scientific, Rockford, IL). cDNA synthesis was performed using EvoScript Universal cDNA Master ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with a protease and phosphatase inhibitor cocktail (Thermo Scientific, Rockford, IL, USA) using a Potter-homogenizator ...
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bioRxiv - Molecular Biology 2021Quote: ... and 50 ng/ml human recombinant M-CSF (ThermoFisher Scientific, Rockford, IL, USA). After six days of PBMC culture ...
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bioRxiv - Immunology 2021Quote: ... and IL-12p70 production were measured using the cytokine kits purchased from Invitrogen and following the instructions from the manufacturer.
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bioRxiv - Cell Biology 2020Quote: ... and murine IL-6 were measured using commercial ELISA kits obtained from Invitrogen, Thermo Fisher Scientific (Lower detection limits ...
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bioRxiv - Cell Biology 2019Quote: ... 1 mM NaF) containing Halt protease cocktail inhibitor (Thermo Scientific, Rockford, IL, USA). Following ten minutes incubation on ice ...
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bioRxiv - Plant Biology 2020Quote: ... Protein concentrations were determined by BCA protein assay (Thermo-Fisher Scientific, Rockford, IL). Protein samples were reduced with 10 mM TCEP and alkylated with 25 mM iodoacetamide before trypsin digestion in 1/40 enzyme/protein ratio at 37°C overnight.
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bioRxiv - Neuroscience 2020Quote: ... Bicinchoninic Acid (BCA) protein assay kit was obtained from Thermo Scientific (Rockford, IL). ProSignal Blotting Film was purchased from Genesee Scientific (El Cajon ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The ELISA was performed using Human IL-1β high sensitivity kit from Invitrogen Inc ...
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bioRxiv - Biophysics 2020Quote: ... and stimulated with 50 U/mL recombinant interleukin-2 (IL-2, Thermo Fisher) and 5 μg/mL phytohaemagglutinin (PHA ...
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bioRxiv - Cancer Biology 2021Quote: ... The same medium with addition of 10ng/ml IL-6 (Gibco, Ref: PHC0066) was used for primary MM plasma cell cultures ...
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bioRxiv - Cell Biology 2021Quote: ... Pierce™ Anti-HA Agarose (26181) were purchased from Thermo Scientific (Rockford, IL). Deubiquitinases (DUBs ...
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bioRxiv - Microbiology 2021Quote: Quantification of IL-1β secretion in cell supernatant was measured by ELISA (Invitrogen, IL-1 beta Mouse Uncoated ELISA Kit ...
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bioRxiv - Microbiology 2021Quote: ... Tryptic peptides were further desalted using C18 spin columns (Thermo Fisher Scientific, IL). Samples were then lyophilized and reconstituted in 0.1% trifluoroacetic acid to be analyzed in quadruplicates without fractionation for quantitation purposes.
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was measured (Pierce BCA Protein Assay, Thermo Fisher Scientific, IL, USA) and a total of 100 µg of protein per sample was subjected for trypsin digestion ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were visualized by chemiluminescence (Pierce Supersignal West Dura, Thermo Scientific, IL, USA) and quantified with ChemiDocTM MP imaging system (BioRad) ...
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bioRxiv - Bioengineering 2020Quote: ... and purified using the MEGAclear Transcription Clean-Up kit (Thermo Fisher, Chicago, IL) as described by Vilarino et al10 ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with protease and phosphatase inhibitor cocktail (PPI; Thermo Scientific, Rockford, IL, USA) and EDTA (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... USA)[49] and 1 μM Höchst 33342 (Thermo Fisher Scientific Inc, Rockford, IL, USA) in PBS and immediately imaged using a Zeiss Imager (larval FB ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ and IL-17A were measured with uncoated ELISA kits from Invitrogen in accordance with manufacturer’s recommendations.