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Citations for Thermo Fisher :
2851 - 2900 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... Protein concentration was determined using the Pierce BCA Protein Assay Kit (Thermo Scientific, Rockford, IL). Protein extracts were mixed with 6X SDS sample buffer (Tris pH 6.8 ...
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bioRxiv - Cell Biology 2020Quote: ... Metabolite levels were normalized to total protein as determined by BCA (Thermo Scientific, Rockford, IL) and fold changes compared to day 4 control samples were calculated.
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bioRxiv - Cancer Biology 2021Quote: ... The expression of recombinant IL-18 was induced by 0.5 mM IPTG (Thermo-Fisher Scientific) for 8 h at 37 °C ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... Total protein in the lysate was quantified via a BCA assay (Thermo Scientific, Rockford, IL). A 4 to 20% gradient Tris-Glycine gel was loaded with 30 μg of protein ...
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bioRxiv - Neuroscience 2022Quote: ... using samples diluted to 1:1000 while interleukin (IL)-6 (Thermo Fisher Scientific, Cat. #BMS625) was evaluated using samples diluted 1:2 ...
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bioRxiv - Immunology 2022Quote: ... 2-mercaptoethanol (RP-10) and 10 ng/ml recombinant human IL-2 (Thermo Fisher Scientific) for 18-22 hours ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated for 3 days in presence of 100 U/mL IL-2 (Gibco) and 1 µg/mL PHA (Remel).
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bioRxiv - Cancer Biology 2022Quote: ... and secondary goat anti-rabbit IgG conjugated with horseradish peroxidase (HRP) (Thermo Fisher Scientific, IL). To detect cell-surface HER2 expression by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: ... were dissected and fixed in Richard-Allan Scientific™ Buffered Zinc Formalin (ThermoFisher, Rockford, IL) overnight (∼15 hr ...
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bioRxiv - Immunology 2020Quote: ... and CD4–PE-Cy5.5 (clone S3.5) and IL-17A–PE (clone eBio64DEC17) from Thermo Fisher Scientific.
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bioRxiv - Microbiology 2021Quote: ... The immunopositive spots were visualized by using chemiluminescent reagent (Thermo Scientific Pierce, Rockford, IL, USA) as directed by manufacturer ...
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bioRxiv - Systems Biology 2021Quote: ... Liquid chromatography separation was accomplished on an Ultimate 3000 RSLCnano (Thermo Scientific, Rockford, IL, USA). Samples were loaded directly onto Acclaim Pepmap® C18 (75 μm × 150 mm ...
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bioRxiv - Cancer Biology 2022Quote: ... protein concentration was determined using Pierce BCA Protein Assay kit (Thermo Fisher Scientific, Rockford, IL) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from CD14+ cells (MagJET RNA kit, Thermo Fisher Scientific, Rockford, IL) using the protocol adapted for tissue (KingFisher Duo machine ...
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bioRxiv - Synthetic Biology 2019Quote: ... The resuspended cultures were distributed into 15 mL polypropylene centrifuge tubes (Thermo Scientific, IL, USA) with a working volume of 5 mL and grown for 24 hour (h ...
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bioRxiv - Biophysics 2019Quote: ... and 1X protease inhibitor added prior to lysis (Thermo Fisher Scientific, Pierce, Rockfrod IL USA). Lysed cells were centrifuged at 10,000X g for 7 min and supernatant was transferred onto packed streptavidin beads and incubated with end-over-end mixing for 1 hr at RT ...
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bioRxiv - Cell Biology 2019Quote: ... were from Proteintech Group Inc (Chicago, IL). pAb against claudin-5 (cat. no. 34-1600) was from Invitrogen and mAb against β-actin (cat ...
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bioRxiv - Bioengineering 2019Quote: ... HRP was detected with 1-Step Ultra TMB-ELISA Substrate Solution (Thermo Scientific; Rockford, IL) and the reaction was stopped with 2N sulfuric acid ...
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bioRxiv - Plant Biology 2019Quote: ... using the SuperSignal West Pico detection system (Pierce-Thermo Fisher Scientific Inc., Rockford, IL, USA).
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bioRxiv - Immunology 2020Quote: ... Then cell supernatant was harvested and the cytokine IL-1β (Catalog # 88-7013-22, Invitrogen) and TNFα (Catalog#88-7324-88 ...
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bioRxiv - Molecular Biology 2021Quote: ... the membrane was visualized by enhanced chemiluminescence kit (Thermo Fisher Scientific Inc., Rockford, IL, USA). The intensity of the bands was measured with a scanning densitometer (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Fractionation was performed with Subcellular Protein Fractionation Kit from Thermo Scientific (#78840, Rockford, IL, USA), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... the plates were emptied and blocked with 300 µL blocking buffer (Thermo Scientific, IL, USA) at RT for 1 hrs ...
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bioRxiv - Microbiology 2020Quote: ... IL-18 and TNF-α levels were determined by ELISA kit (ThermoFisher, for MCP-1) and (Affymetrix eBioscience ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, IL). Cleared lysates were supplemented with protein sample buffer ...
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bioRxiv - Pathology 2021Quote: ... and the fraction was subsequently passed through Detoxi-gel (Thermo Fisher Scientific, Rockford, IL, USA) to remove LPS ...
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bioRxiv - Systems Biology 2020Quote: ... Digested samples were desalted with Pierce Peptide Desalting Spin Columns (Thermo Fisher Scientific, IL, USA). Phosphopeptide enrichment was subsequently performed using PolyMac spin tips (Tymora Analytical ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants were combined and 300 μL of neutravidin agarose resin (Thermo Fisher Scientific, Rockland, IL) was added ...
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bioRxiv - Neuroscience 2019Quote: ... Protein quantitation was performed using bicinchoninic acid (BCA) Protein Assay Kit (Thermo Scientific, Rockford, IL); 100 µg of each sample was used for TMT labeling.
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bioRxiv - Immunology 2020Quote: ... IL-17 and IFN-γ were measured by ELISA Ready-Set-Go kits from Thermofisher and culture supernatants were tested at different dilutions according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: Commercially available direct ELISA kits were performed to determine bovine IL-1β (ESS0027 Invitrogen®) and IL-8 (3114-1A-6 MABTECH ...
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bioRxiv - Molecular Biology 2019Quote: ... Protein concentrations were determined by the BCA Protein Assay Kit (Thermo Fisher Scientific, Rockford, IL). Equal amounts of protein (10-50 µg ...
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bioRxiv - Systems Biology 2020Quote: ... Immunoreactive moieties were detected by chemifluorescent detection (Pierce ECL Plus, Thermo Fisher Scientific, Rockford, IL) of horseradish peroxidase-conjugated (HRP ...
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bioRxiv - Immunology 2019Quote: ... The plates were washed and 100 µl of TMB substrate (ThermoFisher Scientific, Rockford, IL, USA) was added to each well ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were then subjected to qPCR using PowerUp SYBR Green Master Mix (ThermoFisher, Rockford, IL) and the following primer sets detecting three transcripts ...
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bioRxiv - Physiology 2021Quote: ... The samples were acidified with 50% trifluoroacetic acid (TFA, Thermo Fisher Scientific, Rockford, IL, USA) until the pH of the samples was lesser than 2 ...
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bioRxiv - Developmental Biology 2021Quote: Stock solution of doxycycline hyclate (dox) (446060250, Acros Organics, Fisher Scientific, Hanover Park, IL, USA) was made up to final concentration of 1 mg/mL in filter sterilized water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... followed by the addition of 25 mM N-ethylmaleimide (NEM) (Thermo Scientific, Rockford, IL USA) for 20 min at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Pre-cleared samples were then incubated with Streptavidin (SA)-Agarose (Thermo Scientific, Rockford IL USA) and mixed overnight at 4°C ...
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Rbx1 Cullin-Ring ligase E3 and Ube2m neddylation E2 differentially governs the fitness of Treg cellsbioRxiv - Immunology 2020Quote: Samples were analyzed on Orbitrap Fusion Lumos mass spectrometers (Thermo Fisher Scientific, Rockford, IL, USA) coupled with an Easy-nLC 1000 nanoflow LC system (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... and then stained with combinations of Foxp3 (FJK-16s) and IL-13 (eBio13A) from Invitrogen and IL-4 (11B11 ...
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bioRxiv - Immunology 2020Quote: CRP and IL-6 were measured using a high sensitivity ELISA (Life Technologies, Carlsbad CA). Plasma soluble CD14 (sCD14 ...
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bioRxiv - Molecular Biology 2021Quote: ... which are pre-treated with 0.1 N sodium hydroxide (cat. SS255-1, Fisher Scientific, IL), 0.5% 3-aminopropyltrimethoxysilane (cat no ...
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bioRxiv - Cell Biology 2022Quote: ... The AAV titer was measured by qPCR using TaqMan (Thermo Fisher Scientific, Rockford, IL, USA) and reported as genome copies (GC)/ml ...
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bioRxiv - Genomics 2022Quote: ... RNA purity was evaluated with a Nanodrop 2000 spectrophotometer (Thermo Scientific, Hanover Park, IL, USA), and RNA integrity by electrophoresis and an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Microbiology 2022Quote: ... supplemented with 10 % v/v heat inactivated fetal bovine serum (Thermo Fisher Scientific, IL, USA). The culture was incubated for three days at 37 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Immunoreactivity was detected with Super Signal West Pico Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA). ImageJ (Scion Corporation ...
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Caloric restriction prevents inflammation and insulin dysfunction in middle-aged ovariectomized micebioRxiv - Physiology 2023Quote: ... followed by development with chemiluminescent reagents (SuperSignal West Pico PLUS, Thermo Scientific, Rockford, IL, USA) and bands intensities were determined by densitometry scanning using the ImageJ software (National Institutes of Health ...
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bioRxiv - Bioengineering 2022Quote: ... Micro BCA and Pierce BCA protein assay kits were purchased from Thermo Scientific (Rockford, IL). PEG-DET polymer was synthesized by aminolysis of PEG-poly(β-benzyl L-aspartate ...
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bioRxiv - Microbiology 2024Quote: ... The ligation products (5 μl) were transformed into DH10B Competent Cells (Thermo Scientific, Rockford, IL) following the manufacturer’s protocol ...