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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for rno mir 542 5p Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... and real-time PCR system (Applied Biosystems 7500 QuantStudio 6 Pro). The Ct value of target genes obtained from each sample was normalized to housekeeping gene GAPDH and relative gene expression was quantified using 2-ΔΔct values.
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bioRxiv - Developmental Biology 2024Quote: ... on a QuantStudioTM 5 Real-Time PCR System (ThermoFisher: Waltham, MA). Finally ...
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bioRxiv - Developmental Biology 2024Quote: ... using QuantStudioTM 12K Flex Real-Time PCR System (Thermo Fisher Scientific). All samples were normalized according to concentration and pooled ...
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bioRxiv - Molecular Biology 2024Quote: ... in a Step One Plus Real-Time PCR System (Applied Biosystems). The abundance of target mRNAs was normalized by that of Gapdh mRNA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific) (Z ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using a QuantStudio Real-Time PCR system (ThermoFisher) with TaqMan Universal PCR Master Mix (ThermoFisher ...
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bioRxiv - Genetics 2024Quote: ... and a Step One Plus Real-Time PCR system (Applied Biosystems). The sigA gene served as an internal reference for calculating relative expression of genes of interest using the 2−ΔΔCT method (Qin et al. ...
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bioRxiv - Microbiology 2024Quote: ... and run on the StepOnePlus Real-Time PCR System (Thermo Fisher). Default ‘Fast’ RT-qPCR and melt curve settings were utilised ...
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bioRxiv - Genomics 2024Quote: ... a QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, USA), with a 20 µl reaction mixture ...
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bioRxiv - Cancer Biology 2024Quote: ... using the ViiA™ 7 Real Time PCR System (Life Technologies). Primer sequences used are in Supplementary Table 3.
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bioRxiv - Molecular Biology 2024Quote: ... in the 7500 Fast Real-Time PCR System thermocycler (Applied Biosystems). The list of primers used are listed in Supplementary Table 1 and the results were analysed using the ΔΔCt method using 18S as the housekeeping gene.
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bioRxiv - Neuroscience 2024Quote: ... on a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). The comparison of transcript levels was calculated using the cycle threshold (CT ...
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bioRxiv - Cancer Biology 2024Quote: ... on a StepOne Plus Real Time PCR instrument (Thermo Fisher Scientific). UBC was used as reference gene for relative quantification ...
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bioRxiv - Neuroscience 2024Quote: ... on a StepOne Plus Real-Time PCR system (Applied Biosystems 4376600). Relative expression was determined using the ΔΔCt method ...
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bioRxiv - Plant Biology 2024Quote: ... and analysed on a StepOne Real-Time PCR System (Applied Biosystems). Relative levels of WLV RNA accumulation were calculated from triplicate reactions using the ΔΔCt method.
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bioRxiv - Plant Biology 2024Quote: qPCR was performed using QuantStudio 3 Real-Time PCR system (ThermoFisher) with PowerUp SYBR Green Master Mix (ThermoFisher) ...
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bioRxiv - Microbiology 2021Quote: ... The real-time OCR was performed using the ABI 7500 Fast Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) and the SYBR Green PCR Master Mix kit (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... and TaqMan™ PreAmp Master Mix was used for preamplification with subsequent real-time qPCR on StepOne Plus Real-Time PCR system (Applied Biosystems) using TaqMan gene expression Master Mix (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2019Quote: ... The qPCR reaction was carried out using either the 7500 Real Time or the QuantStudio 5 Real Time PCR systems (Applied Biosystems) and the process was 2 minutes at 50°C ...
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bioRxiv - Immunology 2022Quote: ... was used to prepare the reactions and real-time qPCR was performed on a STEP-ONE real-time PCR system (Cat. No. 4376357, Applied Biosystems).
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bioRxiv - Microbiology 2024Quote: ... The extracted DNA samples were then subjected to real-time qPCR using a QuantStudio 3 Real-Time PCR System (Applied Biosystems) with SYBR Green Master Mix (Applied Biosystems) ...
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bioRxiv - Microbiology 2023Quote: ... S8) and two MGE genes were quantified by real-time qPCR using a StepOne Plus real-time PCR instrument (Applied Biosystems). The ARGs represented resistance to tetracycline (tetA ...
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bioRxiv - Microbiology 2023Quote: ... using the SuperScript RT-PCR kit (Invitrogen). They were cloned into the XhoI-AvrII site of the pEOE-attP-eGFP vector using In-Fusion cloning ...
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bioRxiv - Immunology 2023Quote: ... RT-qPCR was performed using PCR master mix (Powerup™ SYBR™ Green Master Mix Catalog: A25741) and real-time PCR system (Applied biosystems™ 7500 Fast Real-Time PCR system). CT values were determined after normalizing to housekeeping gene GAPDH.
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bioRxiv - Genetics 2023Quote: ... qPCR was performed with a RT-PCR detection system (Applied Biosystems, California, USA) using a SYBR Premix Ex Taq Kit (Q711 ...
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bioRxiv - Neuroscience 2022Quote: ... Quantitative real-time PCR analysis was performed using a DyNAmo ColorFlash SYBR Green qPCR kit (Thermo Scientific) in a Step One Plus real-time PCR system (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and a quantitative Real-Time PCR was performed according to the manufacturer’s instructions (MMLV Kit, Life Technologies and Smart SYBRGreen kit ...
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bioRxiv - Microbiology 2024Quote: ... a real time PCR quantification step was performed using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher). Next steps included an emulsion PCR of the pools and loading on 520 chips performed using the automated Ion Chef instrument (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... a real time PCR quantification step was performed using Ion Library TaqMan™ Quantitation Kit (Thermo Fisher). Next steps included an emulsion PCR of the pools and loading on 530 chips performed using the automated Ion Chef instrument (Thermo Fisher) ...
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bioRxiv - Microbiology 2024Quote: ... Real-time SYBR-based PCR (qPCR) was carried out with Power SYBR Green Master Mix kit (ThermoFisher) using 12.5 µL Mix Reagent ...
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bioRxiv - Genomics 2022Quote: ... was used for RSV subtype detection with TaqPath 1-Step Multiplex Master Mix (No ROX) on Applied Biosystems 7500 Real-Time PCR System (ABI, Life Technologies, Waltham, MA) as instructed ...
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bioRxiv - Molecular Biology 2023Quote: The mRNA content of AMPKγ3 and TATA-box binding protein (TBP) was determined by real-time PCR using the QuantStudio 7 Flex detection system (Applied Biosystems, CA, USA) as previously described (33) ...
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bioRxiv - Immunology 2024Quote: ... Protein unfolding reactions and fluorescent signal detection were performed in Applied Biosystems 7900 HT Fast Real-Time PCR machine (Applied Biosystems, Thermo Fisher Scientific). For data analyses ...
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bioRxiv - Physiology 2024Quote: ... on cDNA template with a Bio Rad CFX384-well Real-time PCR Detection System and PowerUp SYBR green Master Mix (ThermoFisher Scientific, catalog #A25742). A list of primer sequences for measuring gene expression are provided in table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... A one-step RT-qPCR reaction will be used to amplify the cDNA using the QuantStudio Real-Time PCR System (ThermoFisher Scientific, Waltham, USA). Each sample will be processed in triplicate with beta actin used as a control gene ...
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bioRxiv - Microbiology 2020Quote: The TaqMan real-time RT-PCR assays were performed by using TaqMan Fast Virus 1-Step Master Mix (Thermo Fisher Scientific, MA, USA). Each 20 μl reaction mix contained 5 μl of 4×Fast Virus 1-Step Master Mix ...
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bioRxiv - Plant Biology 2020Quote: ... RT-qPCR of three biological replications with no-template control (NTC) involved StepOnePlus Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) and FastStart SYBR Green (Roche) ...
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bioRxiv - Molecular Biology 2019Quote: The RT-qPCR analysis was carried out using SDS 2.3 software in 7900HT Fast Real-Time PCR System (Applied Biosystems, Foster City, California). For the detection of mRNA expression ...
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bioRxiv - Cell Biology 2020Quote: ... A total of 10ng cDNA for each sample was used and all RT-qPCR reactions were performed on a ViaA 7 Real-Time PCR System (Life Technologies, Carlsbad, CA) using Brilliant II SYBR Green QPCR Master Mix (Agilent ...
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bioRxiv - Neuroscience 2024Quote: ... RT-qPCR was performed in translucent 96-well plates using the Applied Biosystems 7500-Real-Time PCR System (Applied Biosystems, Waltham, MA, USA). For each well ...
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bioRxiv - Molecular Biology 2024Quote: ... to perform a one-step RT-qPCR in a volume of 20 μl using a QuantStudio3 real-time PCR instrument from ThermoFisher (Waltham, MA, USA). The primers used are provided in the Key Resource Table ...
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bioRxiv - Developmental Biology 2024Quote: ... and analyzed by RT-qPCR using Quant Studio 12K Flex Real Time PCR system version 1.1.2 (Applied Biosystems by Life Technologies, Thermo Fisher Scientific) and validated (efficiency > 1.8 ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of target genes was analyzed via Quantitative real-time (RT) PCR with a QuantaStudio 5 qPCR machine (Applied Biosystems, Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2021Quote: Reverse Transcriptase PCR and Quantitative Real-Time PCR were prepared using the Power SYBR Green Cells-to-Ct Kit (Thermo Scientific) protocol ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was generated using q-Script cDNA Supermix kit (Quanta Biosciences) and quantitative PCR was performed in triplicates on 7500 Fast Real-Time PCR system (Applied Biosystems) with Perfecta SYBR Green Supermix (Quanta Biosciences) ...
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bioRxiv - Cell Biology 2020Quote: ... total RNA was extracted using Trizol with analysis by real time PCR according to manufacturer’s instructions (One-step VERSO SYBR green PCR kit, Thermo Fisher Scientific). Total RNA was extracted from mouse lung tissue using Trizol with analysis of 25 ng of RNA reverse transcribed and complementary DNA was amplified by real time-PCR using a One-step VERSO SYBR green PCR kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Quantitative R T-PCR was performed according to the protocol supplied by the SYBR Green PCR kit using the 7500 Real-Time PCR System (Applied Biosystems). Cycling conditions were 94°C for 30 s ...
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bioRxiv - Microbiology 2022Quote: ... The PCR was performed using the BioRad CFX96 Real-Time PCR cycler using a DyNAmo Flash SYBR green qRT-PCR kit (Catalog no. F415XL; Thermo Scientific).
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bioRxiv - Molecular Biology 2024Quote: qRT-PCR was performed using the Power SYBR Green 1-step kit and the 7500 Fast Real-Time PCR System (Applied Biosystems). Relative expression was calculated using the comparative Ct method with Actin B (ACTB ...
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bioRxiv - Cancer Biology 2023Quote: ... The cDNA was used as a template for quantitative PCR using KAPA SYBR FAST qPCR Kits (KAPA) and 7300 Real-Time PCR System (Applied Biosystems). Expression levels of target genes were normalized using an mRNA level of the β2-microglobulin gene (B2M ...