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Citations for Thermo Fisher :
2501 - 2550 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... The supernatant was then purified using Ni-NTA His affinity agarose (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... 6x-His Epitope Tag Monoclonal Antibody (#MA1-21315, Thermofisher Scientific; dilution 1:500) was used for His-tag staining of the TRPA1 channel in fixed cells for 1 h at RT ...
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bioRxiv - Neuroscience 2022Quote: ... each experiment sample was separated on a Hi-pH column (Thermo Fisher Scientific) according to the vendor’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... then diluted at 1:100 in Hi-Def Azure Media (Fisher Scientific # 3H5000) supplemented with 1% Glucose and various concentrations of peptide in 100 µl in a 96well plate ...
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bioRxiv - Plant Biology 2024Quote: ... synthesized GUN1-FL protein was purified using His-Tag Dynabeads™ (Invitrogen; 10103D) and confirmed by Western blotting using His-tag antibody (Sigma ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... cells were placed in HI buffer containing 1:1,000 CellMask Deep Red (Invitrogen) for 15 minutes at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... Bio-His-FIKK13 was incubated with different quantities of magnetic streptavidin beads (Invitrogen) for 30 min ...
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bioRxiv - Microbiology 2024Quote: ... at a 1:5,000 dilution and mouse anti-His monoclonal antibody (Thermo Fisher Scientific or Abcam ab216773 ...
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bioRxiv - Cell Biology 2024Quote: ... excluding the HI-FBS and supplementing with 0.1% BSA (Life Technologies 15260-037). CHIM was used for the experiments in Figures 4 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 2 μl of Dynabeads™ His-Tag Isolation and Pulldown beads (ThermoFisher 10104D) for each CFPS reaction were equilibrated twice in equal volumes of wash buffer before addition to the purified reactions to remove the Ulp-protease and incubated with spinning at 4 °C for at least 30 minutes ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with an anti-His antibody with HRP conjugate (Invitrogen) in 1% BSA at 4°C overnight with gentle rocking ...
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bioRxiv - Cancer Biology 2023Quote: ... neutralized by adding RPMI-1640 with 10% heat inactivated FBS (HI FBS, Gibco), and spun down at 200 x g for 10 minutes to pellet the cells ...
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bioRxiv - Plant Biology 2023Quote: ... Immunoblotting was performed using anti-His antibody (Thermo Fisher, Cat. No. MA1-21315) at a dilution of 1:10,000 ...
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bioRxiv - Biochemistry 2023Quote: ... supplemented with 10% (v/v) heat-inactivated foetal calf serum (hi-FCS) (Gibco) and 1% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... Western blots were carried out probed with anti-His mAb (1:1000, Invitrogen) followed by anti-mice IgG-HRP (1:20000 ...
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bioRxiv - Cancer Biology 2022Quote: ... medium containing 10% (v/v) heat inactivated fetal calf serum (HI-FCS; Gibco), 100 U/mL Penicillin (Sigma ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10 % v/v heat-inactivated fetal calf serum (HI-FCS, Gibco), 1 mM non-essential amino acids (NEA ...
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 1% (v/v) HI-FBS and N-2 supplement (Gibco™). ATM knockout (KO ...
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bioRxiv - Genetics 2023Quote: ... Libraries were mixed with Novex 5X Hi-Density TBE Sample Loading Dye (Thermofisher), then loaded into a 6% TBE acrylamide gel with the Quick-Load pBR322 DNA-MspI Digest ladder and run for 65 minutes at 120 V ...
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bioRxiv - Biochemistry 2023Quote: ... Successful binding was analyzed using anti-His Tag antibody (PCRD, Invitrogen #MA1-135) or anti-Myc Tag (DCRD ...
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bioRxiv - Developmental Biology 2024Quote: Primary antibodies used were HRP-conjugated anti-His (Invitrogen 46-1009; 1:2000) and mouse anti-FLAG (Sigma F1804 ...
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bioRxiv - Molecular Biology 2024Quote: ... MBP-His-TEV-Epe1 was cloned into a pFastBac vector (Thermo Fisher Scientific) and used for Bacmid generation ...
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bioRxiv - Genetics 2020Quote: ... 0,3 μM primers e one unity of Taq DNA polymerase enzyme (Invitrogen). The reaction was conducted in a final volume of 25 µL ...
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bioRxiv - Genomics 2021Quote: ... Libraries were loaded onto a 2% Agarose E-Gel EX (Invitrogen, G401002) and were excised between 300 bp - 900 bp and cleaned using the Monarch DNA Gel Extraction Kit (NEB ...
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bioRxiv - Genomics 2021Quote: ... Adapter-ligated DNA was separated on a 2% E-Gel EX (Invitrogen) and the 500–800 bp fraction was excised and purified using the QIAquick gel extraction Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... anti-PU.1 antibody (50 μL; Invitrogen Cat #MA5-15064, E.388.3), anti-H3K4me3 (40 μL ...
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bioRxiv - Neuroscience 2021Quote: ... and resuspended in HIB++ media (Hibernate™-E Medium medium (Gibco #A1247601) supplemented with EDTA (1mM final ...
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bioRxiv - Cell Biology 2021Quote: ... medium was switched to William’s E medium (no phenol red, Gibco, A1217601) with Primary Hepatocyte Maintenance Supplements (Gibco ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were removed from embryos and stored in Hibernate-E (Thermo Fisher) overnight during genotyping ...
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A Bidirectional Switch in the Shank3 Phosphorylation State Biases Synapses toward Up or Down ScalingbioRxiv - Neuroscience 2021Quote: ... Cortex was dissected in ice-cold Hibernate E medium (Thermo Fisher Scientific) supplemented with 2% B27 supplement (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were purchased as monolayer cultures and maintained in William’s E (Gibco) supplemented with 1% Glutamine (Gibco) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were maintained in short-term monolayer cultures in William’s E (Gibco) supplemented with 1% Glutamine (Gibco) ...
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bioRxiv - Cell Biology 2022Quote: ... mRNA was polyadenylated using the E-PAP poly(A) tailing kit (Invitrogen). Prior to injection ...
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bioRxiv - Developmental Biology 2020Quote: ... or random hexamers (for Fig. 5c, d & e) (Thermo Scientific™; SO142), dNTP Mix (ThermoScientific™ ...
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bioRxiv - Neuroscience 2021Quote: ... and resuspended in HIBE++ media (Hibernate™-E Medium medium (Gibco #A1247601) supplemented with EDTA (1mM final ...
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bioRxiv - Developmental Biology 2022Quote: ... packaging cell lines Plat-E were transiently transfected using Lipofectamine LTX (Invitrogen) with two expression vectors ...
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bioRxiv - Neuroscience 2022Quote: ... and coverslips (#1.5, 0.175 mm, 12-544-E; Fisher Scientific, Pittsburg, USA).
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bioRxiv - Cell Biology 2019Quote: ... the media was switched to William’s E supplemented with Maintenance Supplements (Invitrogen). Where indicated ...
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bioRxiv - Cell Biology 2019Quote: ... Primary hepatocytes and AML12 cells were cultured in William’s E Medium (Gibco) supplemented with 1X insulin-transferrin-selenium supplement (Gibco) ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Amplicons were run on 4% E-gels (Life Technologies Co., Carlsbad, CA). The risk of having selected primers within repeated portions of the genome had to be considered due to the fact that large repeated genomic fragments are known to occur abundantly in I ...
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bioRxiv - Neuroscience 2021Quote: ... and coverslips (#1.5, 0.175 mm, 12-544-E; Fisher Scientific, Pittsburg, USA).
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bioRxiv - Neuroscience 2020Quote: ... The sections were transferred to 3mL of Hibernate E solution (# A1247601 Gibco) containing 20 U of papain (# LK003178 Worthington-biochemical corporation ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies against E-cadherin (ECCD2) were purchased from Invitrogen (#13-1900). Secondary antibodies from Jackson ImmunoResearch Laboratories were Cy5 AffiniPure Donkey anti-goat IgG (H+L ...
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bioRxiv - Developmental Biology 2021Quote: ... Syrian hamster anti-Pdpn (DSHB) and rat anti-E-cadherin (Thermo Fisher) antibodies as described above ...
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bioRxiv - Immunology 2020Quote: ... after confirming paired amplified product on 96-well E gel (ThermoFisher #G720801). Gibson assembly products were finally transformed into competent E.coli cells and single colonies were picked for sequencing and analysis on IMGT V-Quest online tool (http://www.imgt.org ...
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bioRxiv - Developmental Biology 2022Quote: ... for 10 min using the E-Gel Power Snap Electrophoresis System (Invitrogen). Bands of interest between 150 and 500 bp were excised and purified using the GeneJET Gel Extraction Kit (Cat ...
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bioRxiv - Genomics 2022Quote: ... for 10 min using the E-Gel Power Snap Electrophoresis System (Invitrogen). Bands of interest between 150 and 500 bp were cut out and purified using the GeneJET Gel Extraction Kit (Cat ...
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bioRxiv - Neuroscience 2022Quote: ... then visualised using an E-Gel Safe Imager Real Time Transilluminator (Invitrogen) to ensure specific amplification ...
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bioRxiv - Physiology 2022Quote: ... Primary mouse hepatocytes were cultured in William’s E medium (Life Technologies #12551032) containing 10% FBS and 1% P/S ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Ovarian sections were stained with hematoxylin and eosin (H&E, ThermoFisher Scientific) for histological examination of un-ruptured large antral follicles ...