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Citations for Thermo Fisher :
2301 - 2350 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... and counter-stained with fluorophore-tagged goat anti-mouse IgG (H+L) antibody (Invitrogen), before being acquired with BD FACSCanto II (BD Biosciences) ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... and/or AlexaFluor-594-tagged goat anti-guinea pig IgG (1:500; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Each tagged protein was visualized by 1-Step Ultra TMB-Blotting Solution (ThermoFisher Scientific).
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bioRxiv - Bioengineering 2020Quote: ... sections were incubated with the appropriate Alexa 546-tagged secondary antibody (Invitrogen, Carlsbad, CA) for 2 hrs at room temperature (RT) ...
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bioRxiv - Microbiology 2020Quote: ... SV5-tagged proteins were detected using mouse anti-SV5 mAb (1:5000, Life Technologies); HA-tagged proteins were detected by anti-HA mAb (clone HA-7 ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged RNF219 and CNOT1 cDNAs were cloned into pCDNA5/FRT-TO vector (Invitrogen). The expression vectors were then transfected into HEK-293 Flp-In-TRex cells and followed by selection with hygromycin to generate stable cell lines ...
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bioRxiv - Molecular Biology 2019Quote: ... GST-tagged GSK3β constructs were cloned into the pDEST™27 vector (ThermoFisher Scientific) as described previously66 ...
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bioRxiv - Biochemistry 2021Quote: ... GST-tagged RASSF5 (GST-RASSF5) sequence was transferred into the pDEST15 expression vector (Thermofisher), and then expressed and purified from BL21-A1cells ...
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bioRxiv - Immunology 2022Quote: ... and RAW 264.7 EROS FLAG-tagged cells were maintained in DMEM medium (21969035, ThermoFisher) containing 10 % FBS (F7524 ...
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bioRxiv - Biophysics 2022Quote: ... the cells were transfected with GFP-tagged protein plasmids using Lipofectamine 2000 (Invitrogen, 11668027). Each well was transfected using 100 ng of plasmid and 1 μL of Lipofectamine 2000 in a total of 50 μL of OptiMEM (Gibco ...
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bioRxiv - Immunology 2022Quote: ... was used to generate Flag-tagged Prom1 clones and deletion mutants in pcDNA3.1+ (Invitrogen). The primers used for cloning are listed in Supplementary Table 1.
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bioRxiv - Biochemistry 2023Quote: ... 6xHis-tagged CapZ was purified as previously described30,49 using HisPur Cobalt resin (Thermo Scientific), and separated from high-molecular weight contaminants by size-exclusion chromatography using a Sephacryl S-200 HR column (Cytiva/GE Healthcare) ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Biophysics 2024Quote: ... followed by incubation with secondary antibodies tagged with Alexa fluorophores (Invitrogen, A11034; Invitrogen, A21422). Samples were mounted with media containing DAPI (Southern Biotech ...
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bioRxiv - Neuroscience 2023Quote: ... N2A cells were transfected with different HA tagged WDR47 variants using Lipofectamine 2000 (Invitrogen) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... A fluorescently tagged secondary antibody (Goat anti-rabbit, Alexa Fluor 647, Thermo Fisher Scientific) diluted in the same antibody solution was applied for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... HA-tagged scFvs were detected using mouse mAb 2-2.2.14 (ThermoFisher Catalog# P126183, RRID:AB_10978021). The following day sections were washed 4 x 5 min with vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... and Alexa Fluor 488-tagged donkey-α-goat secondary antibody (1:1000; Molecular Probes) for one hour ...
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bioRxiv - Developmental Biology 2022Quote: ... and tagged Stat3 were synthesized with linearized plasmids using T7 mMessage mMachine kit (Invitrogen). For overexpression essays ...
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bioRxiv - Cell Biology 2023Quote: ... and His6-tagged VASP (Benz et al., 2009) using Lipofectamine 2000 (Invitrogen, Karlsruhe, Germany). Cells were lysed in buffer A (40 mM HEPES-NaOH ...
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bioRxiv - Developmental Biology 2023Quote: ... The primary labelling was detected using appropriate Alexa-Flour tagged secondary antibody (ThermoFisher Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... WRN Halo-tagged U2OS cells were seeded in GlutaMAX- supplemented DMEM (Gibco, 10566-016) with 10% FBS and 1% Penicillin/Streptomycin at 6x103 cells per well ...
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bioRxiv - Biochemistry 2024Quote: NusA-Strep-tagged DDX6 was produced in E.coli BL21(DE3) Star cells (Thermo Fisher) in LB medium at 20°C ...
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bioRxiv - Developmental Biology 2022Quote: ... coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified protein was used to immunize guinea pigs (Cocalico Biologicals).
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bioRxiv - Microbiology 2021Quote: ... and transformation into an Escherichia coli Mach1 (Thermo Fisher Scientific) were performed ...
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bioRxiv - Plant Biology 2019Quote: ... coli One Shot®Top10 cells (Invitrogen Corp., Carlsbad, CA) and purified from an overnight culture using DNA Mini prep kit (Qiagen Co. ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cultures using the PureLink Quick Plasmid Miniprep Kit (ThermoFisher). Input PCR products were purified using the GeneJet PCR purification kit (ThermoFisher) ...
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bioRxiv - Developmental Biology 2019Quote: ... coli strain BL21 and purified on glutathione-agarose (Thermo Scientific). Purified proteins were used to immunize guinea pigs (Cocalico Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... coli DH5-α cells for multiplication (all Thermo Fisher Scientific). Plasmids were purified with the E.Z.N.A ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli cells were grown in LB medium (Fisher Scientific, DF0446173), SOB medium (Teknova ...
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bioRxiv - Neuroscience 2020Quote: ... coli expression vector pDEST15 with GST tag Gateway Vector (Invitrogen). MYC-LRRK2-WT was a gift from Dr ...
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bioRxiv - Microbiology 2020Quote: ... coli K-12 derivate Top10 (Life Technologies, Carlsbad, CA, USA) and standard techniques like Golden Gate as well as Gibson assembly were followed (Gibson et al. ...
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bioRxiv - Cell Biology 2021Quote: ... Lipopolysaccharide (LPS) (from Escherichia coli O11:B4, L4391, Thermo Fisher).
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bioRxiv - Molecular Biology 2022Quote: ... This plasmid was transformed into Escherichia coli TOP10 cells (Invitrogen). Cells were grown at 37 °C to log phase at an OD600 of 1.7-1.9 in Terrific-Broth (TB) ...
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bioRxiv - Microbiology 2022Quote: ... coli strains were cultured in LB broth (Thermo Fisher Scientific) supplemented with 100 mg/L ampicillin (FUJIFILM Wako Pure Chemical Corporation ...
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bioRxiv - Biochemistry 2022Quote: ... coli and obtained as a synthetic gene from GeneArt (Thermofisher). Full-length LiRAD51 was cloned into pExp-MBP plasmid (Dr Aleksei Lulla ...
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bioRxiv - Microbiology 2022Quote: ... These constructs were synthesised by Genscript (Netherlands) and expressed in Escherichia coli BL21 (DE3) cells (Invitrogen). E ...
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bioRxiv - Biochemistry 2021Quote: ... coli Bl21 (λDE3) and DH5α strains were supplied by ThermoFisher Scientific ...
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bioRxiv - Microbiology 2019Quote: ... coli with the TA Cloning Kit (Life Technologies, Carlsbad, VS) and plasmid extracted with the PureYield Plasmid Miniprep System (Promega ...
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bioRxiv - Biochemistry 2019Quote: ... coli BioParticles™ Conjugate for Phagocytosis (Thermo Fisher Scientific, USA) was added ...
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bioRxiv - Molecular Biology 2020Quote: ... and transformed into Escherichia coli BL21(DE3) pLys cells (Invitrogen) for expression ...
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bioRxiv - Plant Biology 2020Quote: ... coli BL21 (DE3)pLysS cells (Invitrogen, La Jolla, CA, USA) were transformed with plasmid DNA ...
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bioRxiv - Microbiology 2020Quote: ... coli DH5α (Subcloning Efficiency DH5α Competent cells, Invitrogen, United Kingdom), A ...
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bioRxiv - Pathology 2021Quote: ... coli competent cells (AMP resistant) (Thermo Fisher Scientific, Waltham, Ma). RNA PCR was performed using T7 ...
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bioRxiv - Biophysics 2019Quote: ... coli BL21-AI chemically competent cells (Invitrogen™, Cat# C607003) using plasmids listed in Table S1 and purified using the N-terminal StrepII tag on Cas1 as described (Kunne et al. ...
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bioRxiv - Evolutionary Biology 2020Quote: ... coli cultures using the PureLink Genomic DNA Mini Kit (Invitrogen). For each sample ...
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bioRxiv - Cell Biology 2020Quote: ... coli using a PureLink HiPure Plasmid Miniprep Kit (Thermo Fisher) and used to transfect Sf9 cells using Cellfectin II transfection reagent (Thermo Fisher) ...
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bioRxiv - Biophysics 2021Quote: Transforming the chaperone constructs in Escherichia coli DE3 cells (Invitrogen) provided us selected colonies which were then grown in luria broth media with respective antibiotics (ampicillin and carbenicillin 50 µg/ml ...