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Citations for Thermo Fisher :
2351 - 2400 of 5507 citations for Recombinant E. coli LT IIc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... and transformed in Escherihia coli BL21(DE3) pLys cells (Invitrogen). Cells were grown in LB medium at 37°C and over-expression of protein was induced with 1 mM IPTG overnight at 18°C ...
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bioRxiv - Microbiology 2020Quote: ... expression in Escherichia coli strain BL21 Star DE3 PlysS (Invitrogen), and purification from inclusion bodies were performed as previously described [33 ...
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bioRxiv - Immunology 2021Quote: ... coli clones using a GeneJET plasmid miniprep kit (Thermo Fisher), and DNA was sequenced using a Sanger sequencer ...
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bioRxiv - Immunology 2021Quote: ... coli DNA polymerase I and DNA ligase (Thermo Fisher Scientific). Sequencing libraries were prepared with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) ...
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bioRxiv - Cell Biology 2021Quote: ... coli strain BL21 (de3) pLysS (C606003, Thermo Fisher Scientific, USA). GST-fused LRRC23 recombinant protein was expressed and subjected to the treatment of PreScission Protease to remove the GST tag [41] ...
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bioRxiv - Molecular Biology 2022Quote: ... coli with either PureLink™ HiPure Plasmid Midiprep Kit (Invitrogen) or PureLink™ HiPure Plasmid Miniprep Kit (Invitrogen) ...
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bioRxiv - Molecular Biology 2022Quote: ... Libraries were then transformed by electroporation into electrocompetent coli (Invitrogen) and spread onto bioassay plates ...
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bioRxiv - Microbiology 2024Quote: ... coli SM10 were grown in LB broth (Fisher Scientific BP97235) or LB+1.5% agar supplemented when needed with carbenicillin (100 µg/mL ...
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bioRxiv - Immunology 2024Quote: ... coli BioParticles and pHrodo S.aureus BioParticles (ThermoFisher Cat.# P35361, A10010) were incubated for 60min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... coli BL21 (DE3) with Pierce Glutathione Agarose (Thermo Fisher Scientific), and incubated in reaction buffer (50 mM HEPES-NaOH ...
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Efficient Methods for Target Gene Manipulation in Haematopoietic Stem Cell Derived Human NeutrophilsbioRxiv - Cell Biology 2023Quote: ... pHrodo-red labelled Staphylococcus aureus or Escherichia coli bioparticles (Invitrogen) were serum opsonised for 30 minutes in 50% human serum and then used in-assay at a concentration of 25 µg/mL ...
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bioRxiv - Neuroscience 2023Quote: ... coli (BL21) and purified using glutathione sepharose 4B (Fisher Scientific). Peak fractions were concentrated and further purified by gel filtration as previously described11 ...
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bioRxiv - Molecular Biology 2023Quote: ... coli TOP10 cells was from a commercial source (Invitrogen, C404010). MC4100 strains were from a previous study65 ...
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bioRxiv - Systems Biology 2023Quote: ... coli MegaX DH10B T1R cells (Invitrogen, Thermo Fisher Scientific Inc.).
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bioRxiv - Systems Biology 2023Quote: ... coli MegaX DH10B T1R cells (Invitrogen, Thermo Fisher Scientific Inc.).
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bioRxiv - Molecular Biology 2023Quote: ... Ligation products were transformed into Escherichia coli TOP10F’ cells (Invitrogen) and grown on LB plates supplemented with carbenicillin (100 µg/ml ...
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bioRxiv - Genomics 2023Quote: ... coli ElectroMAXTM DH5α-ETM electro-competent cells (Thermo Fisher Scientific), as indicated by the authors (Adamson et al ...
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bioRxiv - Cell Biology 2023Quote: ... coli strains used in this study include Mach1T1R (Thermo Fisher), BL21 (DE3)T1R (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... coli colonies using the GeneJET Plasmid Miniprep Kit (ThermoFisher Scientific). The variable region of the library (position 19 to 83 ...
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bioRxiv - Biochemistry 2023Quote: ... coli RNase I (Ambion, 750 U per 100 A260 units) by incubation at 4 °C for 1 h ...
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bioRxiv - Biophysics 2023Quote: ... coli BL21 Star (DE3) star cells (Invitrogen, Carlsbad, CA, USA).
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bioRxiv - Biophysics 2023Quote: ... coli One Shot™ BL21 Star™ (DE3) cells (Invitrogen). Freshly transformed cells were grown on M9 agar containing 1 g/L 1NH4Cl ...
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bioRxiv - Plant Biology 2023Quote: ... Plasmid was transformed into Escherichia coli electrocompetent cells (Invitrogen, USA) following the manufacturer’s provided protocol ...
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bioRxiv - Plant Biology 2023Quote: ... coli using the One Shot® TOP 10 kit (Invitrogen). The transformed cells were plated with ampicillin (100 μg/mL ...
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bioRxiv - Neuroscience 2024Quote: ... coli BioParticles™ Conjugate for Phagocytosis (ThermoFisher Scientific,Cat#: P35366) were resuspended in 2ml PBS (Corning ...
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bioRxiv - Biochemistry 2024Quote: ... pETM41-naa80 was introduced into Escherichia coli BL21* cells (Invitrogen) by heatshock transformation.
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bioRxiv - Microbiology 2023Quote: ... coli DH5α cultures using the GeneJET Plasmid Miniprep (Thermo Scientific), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... coli using the Purelink HiPure Plasmid Midiprep Kit (Thermo Fisher).
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bioRxiv - Plant Biology 2024Quote: ... coli cells using the GeneJet Plasmid MiniPrep kit (ThermoFisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... coli strains used in this study include Mach1T1R (Thermo Fisher) and BL21 (DE3)T1R (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... coli strains were from Thermo Scientific (Cat. No. EC0111, EC0114).
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH5α cells for cloning were obtained from Thermofisher (#EC0112) and competent E ...
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bioRxiv - Cell Biology 2020Quote: ... 20 ng/ml recombinant human epidermal growth factor (Invitrogen, USA), 10 ng/ml human basic FGF (Invitrogen ...
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bioRxiv - Cell Biology 2020Quote: ... human recombinant Epidermal Growth Factor (5 ng/ml, ThermoFisher Scientific) and 0.15 mM CaCl₂ (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2020Quote: ... coated with 5μg/ml human recombinant vitronectin (Life Technologies, #A14700) in mTesR1 (StemCell Technologies ...
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bioRxiv - Bioengineering 2022Quote: ... 0.36 pM Recombinant Human Bone Morphogenetic Protein 4 (BMP4, Gibco), and 0.08 pM Activin A (Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... and human recombinant epidermal growth factor (EGF; Gibco 10450-013). All cells were cultured at 37 °C and 5% CO2.
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bioRxiv - Immunology 2021Quote: All recombinant proteins were produced using Expi293F cells (Life Technologies). Spike proteins for enzyme-linked immunosorbent assay (ELISA ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2019Quote: ... and recombinant epidermal growth factor (10 ng/ml; Gibco-Invitrogen) at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2020Quote: Glial-Derived Neurotrophic Factor (GDNF) Recombinant Human Protein (ThermoFisher, #PHC7044)
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bioRxiv - Neuroscience 2020Quote: Brain-Derived Neurotrophic Factor (BDNF) Recombinant Human Protein (ThermoFisher, #PHC7074)
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Virus-mediated transient expression techniques enable genetic modification of Alopecurus myosuroidesbioRxiv - Genetics 2020Quote: ... with RNaseOUT™ Recombinant Ribonuclease Inhibitor (Invitrogen cat# 10777-019).
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bioRxiv - Cell Biology 2021Quote: ... RNaseOUT recombinant ribonuclease inhibitor (200 U/mL; Thermo Fisher Scientific) and IGEPAL® CA-630 (0.5 % ...
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bioRxiv - Immunology 2021Quote: ... and recombinant epidermal growth factor (EGF, 2 ng/ml, Gibco).
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bioRxiv - Bioengineering 2020Quote: ... 12.5μg/ml Insulin human recombinant zinc solution (Thermo Fisher 1258014)] ...
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bioRxiv - Biochemistry 2020Quote: ... 40 U RNase inhibitor (RNaseOUT ™ Recombinant Ribonuclease Inhibitor, Invitrogen), and various amount of DNA sample (see figures) ...
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bioRxiv - Biophysics 2021Quote: Recombinant constructs were expressed in E.coli BL21 DE3 cells (Invitrogen) in Luria Bertani media ...
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bioRxiv - Neuroscience 2019Quote: ... 200 units/ml RNaseOUT™ Recombinant Ribonuclease Inhibitor (#10777019; Thermofisher), and 1X cOmplete™ ...
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bioRxiv - Biochemistry 2021Quote: ... Recombinant protein was expressed using Expi293F™ Cells (ThermoFisher Scientific) following manufacturer’s protocol ...