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Citations for Thermo Fisher :
2551 - 2600 of 10000+ citations for rno mir 143 RT PCR Primer Set since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... oligo-dT primers (Invitrogen) and RNase inhibitor (Applied Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... and random primers (Invitrogen). Quantitative PCR was performed using SYBR Green (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... and random primers (Invitrogen). Quantitative PCR was performed using SYBR Green (Applied Biosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... either Random Primers (Invitrogen) or gene-specific primers were employed in conjunction with Reverse Transcriptase (Roche Applied Science) ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5µM reverse primer (ThermoFisher), and reactions conducted using a 3 step protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Taqman primers (Life Technologies) were used ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5µM forward primer (ThermoFisher), 0.5µM reverse primer (ThermoFisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... 100 nM of miR-21-5p inhibitor (Thermo Fisher Cat#4464084 ID#MH10206 Lot#AS02DR3S) or negative control mimic (Thermo Fisher Cat#4464058 Lot#AS02CNS6 ...
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bioRxiv - Cancer Biology 2022Quote: ... Biotin-labelled miR-2861 (3 μg) was incubated with streptavidin-coated beads (434341; Thermofisher Scientific) at 4□°C overnight ...
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bioRxiv - Cell Biology 2021Quote: ... PLGA nanoparticles incorporating a mirVana miRNA mimic hsa-mir 132-3p (4464066, Thermo Fisher Science) were prepared using an emulsion solvent diffusion method in RNAase-free water ...
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bioRxiv - Molecular Biology 2021Quote: Primary transcript pri-miR-K10/12 was analyzed after prior treatment with DNase I (Invitrogen) or TURBO™ DNase (Invitrogen) ...
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bioRxiv - Cell Biology 2021Quote: ... 100 nM of miRVANA miR-184 inhibitor (cat. 4464084, ID. MH10207-Invitrogen, Pittsburgh, MO, USA) + 50 nM of CTR siRNA ...
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bioRxiv - Cancer Biology 2022Quote: ... The miR-28 precursor sequence was cloned into the doxycycline-inducible pTRIPZ vector (Thermo scientific), and a scramble pTRIPZ vector was used as a control ...
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bioRxiv - Immunology 2023Quote: ... The tissue constructs used for miR-2392 mimics were dissolved in TRIzol (ThermoFisher Cat# 15596026) 48 hours post-transfection ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Immunology 2023Quote: ... or the appropriate miR mimic or inhibitor scramble control at 100nM (ThermoFisher Scientific, Waltham, MA). Transfections were confirmed by reverse transcription quantitative polymerase chain reaction (RT-qPCR ...
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bioRxiv - Cancer Biology 2023Quote: miR-E shRNA knockdown constructs were generated by the splashRNA program and ordered from Invitrogen as oligos ...
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bioRxiv - Cell Biology 2024Quote: ... we used a Block-iT Pol II miR RNAi expression vector kit (Thermo Fisher Scientific). The targeting sequence of Tmem63b (5′- CGAGGACAGCCTCATAGAGAA-3′ ...
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bioRxiv - Cell Biology 2024Quote: ... anti-hsa-miR-221-3p or anti-miRNA negative control using LipofectamineTM 2000 (ThermoFisher Scientific) for 48 hours ...
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bioRxiv - Neuroscience 2024Quote: ... The detection of miR-124 was performed adhering to manufacturer’s instructions from Taqman™ (Invitrogen) with the U6 snRNA 3p (Assay ID ...
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bioRxiv - Plant Biology 2023Quote: ... sequencing libraries were generated using indexed primers and enrichment primers (IDT Europe, see Primer List) with Phusion HF polymerase (Thermo Fisher). Libraries were visually inspected on an agarose gel before a double clean up with Ampure XP magnetic beads (Beckman) ...
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bioRxiv - Microbiology 2020Quote: ... Northern blot probes were synthesized in vitro by engineering probe-specific PCR primers to contain a T7 promoter then utilizing a MAXIscript T7 Transcription kit (Invitrogen) supplemented with 0.5 mM Bio-16-UTP (Invitrogen) ...
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bioRxiv - Plant Biology 2020Quote: ... the coding sequence of tomato MYC1 was PCR-amplified with the primers listed in Supplemental Table 1 and recombined in a Gateway donor vector (Invitrogen). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... with gene-specific primers according to the manufacturer’s protocol and with the Applied Bioscience StepOnePlus qRT-PCR machine (Life Technologies). The expression levels were normalized to the endogenous 18S rRNA expression using the delta-delta comparative threshold method (ΔΔCT) ...
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bioRxiv - Genetics 2021Quote: ... qRT-PCR analysis was then performed with gene specific primers and Applied Biosystems Power SYBR master mix (Thermo Fisher Scientific) with ACTB house-keeping gene as an internal control ...
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bioRxiv - Microbiology 2021Quote: ... primers remaining from PCR amplification were removed by treatment with ExoSAP (Exonuclease I, Shrimp Alkaline Phosphatase; Thermo Scientific; https://www.fishersci.fi/). Assembled complete ITS regions (ITS1 - 5S rRNA - ITS2 ...
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bioRxiv - Molecular Biology 2021Quote: ... coli DY330 genomic DNA (for primers see Table S1) and purified with GeneJET Gel Extraction and DNA cleanup micro kit (PCR cleanup protocol, ThermoFisher). For binding 2.85 pmoles of DNA fragment was mixed with increasing amount of purified EcTopoI (0-16 pmoles and 0-32 pmoles correspondingly ...
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bioRxiv - Genetics 2020Quote: ... 50ng DNA from the positive or negative control samples were PCR amplified (30 cycles) using primers that eccentrically flanked the predicted cut-site (Phusion High Fidelity Polymerase, ThermoFisher). T7 endonuclease I digestion was then performed according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... and 0.5 μM of gene-specific primers (Table S2) in a 7500 Fast Real-Time PCR System (Thermo Fisher Scientific), using a two-step cycling protocol (3 seconds at 95°C ...
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bioRxiv - Molecular Biology 2019Quote: BGH was amplified by PCR from pslVg-GFP-BGH by PCR with BamHI and NotI tagged primers (BGH-F, BGH-R) and subcloned into pJET1.2 (Thermo Scientific). attP was amplified from pBCPB+ (Groth et al ...
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bioRxiv - Developmental Biology 2019Quote: ... were mixed with 0.45 μM Tail primer (Extended Data Table 2) and PCR amplified with Platinum Pfx DNA polymerase (ThermoFisher Scientific) in a T100 thermal cycler (BioRad) ...
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bioRxiv - Immunology 2019Quote: ... PCR products were sequenced with the reverse primer CHG-D1 and read on ABI PRISM 3130XL genetic analyser (Applied Biosystems). Sequence quality was verified with the CodonCode Aligner software (CodonCode Corporation) ...
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bioRxiv - Plant Biology 2021Quote: ... The substitution mutations in βC1 were generated using overlapping PCR primers harbouring the required modification or by site-directed mutagenesis kit (Invitrogen). Primer and plasmid used in the study are detailed in (Tables S2 ...
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bioRxiv - Genetics 2020Quote: ... mCherry was first PCR-amplified from vector pLEXY_IE-blecherry4 (JenaBioscience) using primers 127 and 128 and cloned into pCR 2.1 TOPO (Thermo Fisher Scientific) to generate pCR 2.1 TOPO-mCherry ...
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bioRxiv - Immunology 2021Quote: Parasite burden was measured via qRT-PCR using primers that recognise P.berghei specific sequences within the 18S rRNA and SYBR Green (Applied Biosystems) as outlined previously [37] ...
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bioRxiv - Plant Biology 2020Quote: ... Genomic sequences were PCR amplified using gene specific primers listed in Table S2 and cloned into pENTR/D-TOPO (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Plant Biology 2020Quote: ... As a control GFP or MBP alone was also cloned into pBIN19 vector which was used a vector control (pBIN).The substitution mutations in βC1 were generated using overlapping PCR primers harbouring the required modification or by site directed mutagenesis kit (Invitrogen). For recombinant protein expression in E ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 30 ng cDNA was used as a template in qPCR reactions with 0.5 µM of forward and reverse primers 51 and Power SYBR Green PCR Master mix (Applied Biosystems). Control samples were prepared that lacked reverse transcriptase to confirm genomic DNA was eliminated from samples ...
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bioRxiv - Molecular Biology 2020Quote: ... Excision was further confirmed by RT-PCR analysis of the first-strand cDNA produced from total mRNA with oligo-dT primers with SuperScript II polymerase (Invitrogen).
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bioRxiv - Microbiology 2020Quote: ... Quantitative PCR was performed using previously described gene specific primers in an ABI7300 Real Time PCR system with SYBR Green Master Mix (Invitrogen) (12).
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bioRxiv - Cancer Biology 2021Quote: ... pcDNA3-RNF43-HA was prepared by subcloning RNF43 PCR product containing HA encoding sequence in reverse primer to the pcDNA3 backbone (Invitrogen). All obtained plasmids were verified by the Sanger sequencing method.
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bioRxiv - Cancer Biology 2022Quote: ... They have been regularly screened for mycoplasma infection using a PCR-based method with the primers Myco1 (5’-GGCGAATGGGTGAGTAACACG) and Myco2 (5’-CGGATAACGCTTGCGACTATG) (Invitrogen) and no cultures have tested positive.
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bioRxiv - Systems Biology 2022Quote: ... Reverse transcription with random primers and subsequent PCR were performed with TaqMan RNA-to-CT 1-Step Kit (Applied Biosystems). Raw data for cycle threshold (Ct ...
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bioRxiv - Plant Biology 2021Quote: ... and SlSAP was PCR-amplified with the primers listed in Supplemental Table 2 and recombined in a Gateway donor vector (Invitrogen). Gateway donor vectors containing the coding sequence of tomato SlTPL1–6 were obtained from (Hao et al. ...
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bioRxiv - Microbiology 2021Quote: ... and previously published TaqMan primers and probe (Corman et al., 2020) on a StepOnePlus RealTime PCR System (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: Two hundred nanogram purified tissue DNA was subjected to quantitative real-time PCR with KSHV ORF73 primers and SYBR Green Master Mix (ThermoFisher) on LightCycler 480II (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... the genes coding for relevant enzymes were amplified from PA01 genomic DNA by PCR with primers 58-68 & 71-72 and sub-cloned into a pET101 Directional TOPO vector (Invitrogen) allowing protein production with an N-terminal 6×His-tag ...
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bioRxiv - Molecular Biology 2020Quote: ... Primer and probe concentrations were optimised according to suggestion in the manufacturer’s instructions (TaqMan Universal PCR mastermix, Applied Biosystems, USA).
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bioRxiv - Immunology 2021Quote: ... PCR products were sequenced with the reverse primer CHG-D1 and read on ABI PRISM 3130XL genetic analyzer (Applied Biosystems). Sequence quality was verified with the CodonCode Aligner software (CodonCode Corporation).