Labshake search
Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2021Quote: ... the co-cultures of MitoTracker CMXROS labeled primary E15 neurons and astrocytes derived from adult WT or 5xFAD mouse brain were first fixed with 4% formaldehyde and then labelled with Alexa Fluor 488 Phalloidin dye (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2022Quote: ... Incubations were performed for 14h at 4° C followed by incubation with secondary antibodies: Alexa 568 anti-mouse 1:200 (Thermo Fisher A10037), and Alexa 488 anti-goat 1:200 (Thermo Fisher A11055 ...
-
bioRxiv - Genetics 2019Quote: ... Each sample containing equal amount of proteins was incubated overnight at 4 ℃ with mouse anti-V5 (1:500; Thermo Scientific, R960-25). Then ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: Brains were washed again as before and then incubated for 3h at room temperature followed by 24h at 4 °C with Alexa Fluor Plus 488 Goat anti-mouse (Life Technologies # A32723) and Alexa Fluor Plus 555 goat anti-rabbit (Life Technologies # A32732 ...
-
bioRxiv - Immunology 2020Quote: ... The cells were washed twice with FACS buffer and then incubated with 50 μL of 4 μg/ml goat anti-mouse IgG-Alexa Fluor 488 (Thermo Fisher Scientific) in FACS buffer for 30 min on ice ...
-
bioRxiv - Cell Biology 2021Quote: ... at 4°C overnight and then incubated with horseradish peroxidase (HRP)-conjugated secondary anti-mouse or rabbit antibodies (Thermo Fisher, Waltham, MA). Protein expression was detected by using enhanced chemiluminescence (ECL ...
-
bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, Invitrogen, USA; 1:500 dilution) and AF488-conjugated α-bungarotoxin (B13422 ...
-
bioRxiv - Physiology 2021Quote: ... and then incubated overnight at 4°C with AF594-conjugated goat anti-mouse IgG1 secondary antibody (A21125, ThermoFisher, USA; 1:500 dilution), Cy5 goat anti-rabbit IgG (H&L ...
-
bioRxiv - Animal Behavior and Cognition 2020Quote: ... the brains were rinsed in PBS for 4 × 15 min before being incubated in the Cy2-conjugated anti-mouse secondary antibody (dilution 1:300 in PBSX; Invitrogen, Eugene, OR), at 4 C for 1 d ...
-
bioRxiv - Genomics 2021Quote: ... Cat# ABIN6952432) overnight at 4 ?C followed by staining with Alexa Fluor 647-conjugated secondary antibody (goat anti-mouse, Thermo Fisher, A21235) and DAPI (Thermo FIsher) ...
-
bioRxiv - Neuroscience 2022Quote: ... Secondary antibody incubation was also performed overnight at 4°C with donkey anti-mouse Alexa 647 (1:250, Invitrogen, Cat. # A-31571).
-
bioRxiv - Neuroscience 2022Quote: ... Slices were incubated overnight at 4°C in a 1:500 dilution of primary antibody: Tyrosine Hydroxylase (TH, mouse, Invitrogen; MA1-24654) and the immediate early gene protein c-Fos (c-Fos ...
-
bioRxiv - Neuroscience 2022Quote: ... slices were incubated overnight at 4°C with goat anti-mouse Alexa Fluor 568 and goat anti-rabbit Alexa Fluor 488 (ThermoFisher A-11008), both diluted 1:500 in blocking solution ...
-
bioRxiv - Biophysics 2022Quote: ... rinsed 3 times in PBT and incubated for 4 hours at room temperature with anti-mouse secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific) at 1:1,000 in PBT for 4-5 hours ...
-
bioRxiv - Neuroscience 2024Quote: ... Sections were washed 3 x 7 min with PBS and incubated overnight at +4°C with goat anti-rabbit Alexa Fluor 594 and goat anti-mouse Alexa Fluor 488 (each 1:1000, Invitrogen, ThermoFisher Scientific) in carrier solution ...
-
bioRxiv - Microbiology 2024Quote: ... for HSV-1 in blocking buffer for 1 h at 4°C and stained with secondary antibody goat anti-mouse/rabbit AlexaFuor680 (Thermo Fisher Scientific) in blocking buffer at RT for 1h ...
-
bioRxiv - Neuroscience 2024Quote: ... Sciatic nerves of 6 mouse pups between postnatal days 2 and 4 (P2 and P4) were collected in L15 medium (Thermofisher, Massachusetts, USA) and incubated for 30 min in 2 mg/mL collagenase II and then 10 min in 0.25 % trypsin containing EDTA at 37 °C ...
-
bioRxiv - Neuroscience 2023Quote: ... slices were incubated in the dark for 4 hr in AlexaFluor-donkey anti-rabbit 594 and donkey anti-mouse 488 (Thermo Fisher Scientific), washed ...
-
bioRxiv - Immunology 2022Quote: ... and 10mM HEPES at 4°C for 18h and revealed with fluorochrome-conjugated anti-mouse and IgG1 and IgG2b antibodies (Thermo Fisher Scientific) for 1 hour at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... T-cells were incubated overnight at 4 °C with either mAb reactive to mouse phospho-CD247 (CD3 zeta) (Tyr111, Tyr123) (clone EM-55; Thermo Fisher Scientific) or mAb reactive to mouse ZAP70 phosphorylated at position 319 (clone 1503310 ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples were resolved by SDS-PAGE using a 4–15% gradient gel and analyzed by Western blot using the following primary antibodies: mouse anti-ZO1 (Invitrogen, 33–9100) and rabbit anti-Cx34.1 3A4 (Fred Hutch Antibody Technology Facility ...
-
bioRxiv - Biochemistry 2023Quote: ... then resuspended in 100 µL of secondary antibody (1:500 α-mouse IgG-Alexa647 [ThermoFisher, Waltham, MA] in TBST with 4% BSA), and incubated in the dark for 60 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... at 4°C overnight and then alkaline phosphatase (AP) conjugated anti-mouse IgG adsorbed with rat serum protein) (Thermo Fisher Scientific, USA) for 2 h diluted 1 ...
-
bioRxiv - Cell Biology 2023Quote: ... primary antibodies in 10% GS in 1xPBS overnight at 4°C followed by a 1h incubation with anti-mouse (A11003, Invitrogen, 1:800) secondary antibody in 10% GS in 1xPBS at room temperature ...
-
bioRxiv - Zoology 2023Quote: ... stained with 4′,6-diamidino-2-phenylindole (DAPI) (1:2000 dilution) and Alexa Fluor-conjugated donkey-anti-mouse (Molecular Probes, 1:1000), in PBST at room temperature for 2 hours ...
-
bioRxiv - Genetics 2023Quote: ... before being incubated at 4°C in Odyssey Blocking Buffer (PBS) containing mouse anti-V5 antibody (RRID: AB 2556564, R960-25; Thermo Fisher Scientific) at a 1:3000 dilution for detection of 3v5 tagged alleles of Ume6 ...
-
bioRxiv - Molecular Biology 2023Quote: ... Cells were washed in PBS three times for 5 min at 4 °C by rocking and then incubated by rocking with secondary antibody (Goat-anti-Mouse 488, 1:2000, Invitrogen #A-11008) in 1% BSA PBST for 1 hour at RT in the dark ...
-
bioRxiv - Neuroscience 2023Quote: ... ENSMUST00000088552.7 and ENSMUST00000034590.4) were amplified by touchdown PCR from a mouse brain cDNA library by Phusion Hot Start II DNA Polymerase (Thermo Fisher, F549L) and cloned into the NheI/SalI restriction sites (RS ...
-
bioRxiv - Cell Biology 2024Quote: ... rinsed 3 times in PBT and incubated for 4 h at room temperature with anti-mouse or anti-rat secondary antibody coupled to Dylight 488 (Thermo Fisher Scientific), or an secondary alpaca nanobody anti-mouse 488 SMS1AF488-1-100 (Thermo Fisher Scientific ...
-
bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
-
bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
-
bioRxiv - Microbiology 2019Quote: ... lipid membranes were incubated with a 6x-His tag polyclonal antibody HRP conjugate (MA1-21315, ThermoFisher) in blocking buffer for one hour at room temperature followed by three washes with wash buffer ...
-
bioRxiv - Genomics 2019Quote: ... Hi-C DNA bound to capture RNA was isolated using a DynaMag-2 magnet (Life Technologies). Washes (15 min in 500 μl wash buffer I at room temperature ...
-
bioRxiv - Molecular Biology 2021Quote: ... Completed reactions were ethanol precipitated and re-suspended in deionized formamide (Hi-Di formamide, Thermo Fisher) with the G-ladders ...
-
bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
-
bioRxiv - Biochemistry 2020Quote: ... optiMEM (1X) reduced serum medium and heat-inactivated fetal bovine serum (HI-FBS) were from Gibco chemicals ...
-
bioRxiv - Neuroscience 2021Quote: ... and labeled with 6x-His Tag Antibody coupled to Alexa 555 (Thermo Fisher, MA1-21315-A555). Coverslips were mounted on slides (Aqua-Poly/Mount ...
-
bioRxiv - Plant Biology 2021Quote: ... BgtCDA1 coding sequences with a 6XHIS tag were amplified and ligated into pPIC9K-HIS vector (Invitrogen), and then transformated into Pichia pastoris strain GS115 (Invitrogen) ...
-
bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
-
An alternatively spliced TREM2 isoform lacking the ligand binding domain is expressed in human brainbioRxiv - Molecular Biology 2022Quote: ... All cloning was performed using a pcDNA 3.1-V5/His TOPO-TA cloning kit (Invitrogen K480001) per manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2022Quote: ... This mix was added to 50 µl pre-washed His-Pur magnetic Nickel beads (Thermofisher Scientific) and incubated for 1 h at 4 °C on a rotor ...
-
bioRxiv - Microbiology 2020Quote: ... NR-52285) using the primers in Table II and cloned into pCDNA-V5/His TOPO (Invitrogen). We introduced a FLAG tag to JUNV GP by PCR amplifying it from a previously described expression plasmid (58 ...
-
bioRxiv - Developmental Biology 2022Quote: S2 cells were transfected with indicated plasmids or blank pMT-V5-His vector (Invitrogen, Cat# V412020) using the Effectene transfection reagent (Qiagen ...
-
bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
-
bioRxiv - Microbiology 2021Quote: ... an 8×His-tag and a Twin-Strep-tag were produced in ExpiCHO-S cells (ThermoFisher) as described previously [10].
-
bioRxiv - Cell Biology 2019Quote: ... The his-tag was removed by cleavage overnight with AcTEV™ protease (Invitrogen, Carlsbad, California, USA) at 4ºC according to the provider’s protocol.
-
bioRxiv - Biochemistry 2021Quote: 1-2 μg His-tagged calcineurin was first bound to magnetic Dynabeads (Thermo Fisher Sci. USA) in base buffer (50 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Immunology 2021Quote: ... with 10% FBS (Nucleus Biologics, #AU FBS-500ml L1 HI) and 1% Penicillin-Streptomycin (Gibco, #15140122) and transferred to a new tube ...
-
bioRxiv - Neuroscience 2024Quote: ... The buffer was then exchanged to the wash solution (10 % Hi-DiTM Formamide (Thermofisher scientific; 4311320) in 2x saline sodium citrate (SSC) ...
-
bioRxiv - Cell Biology 2023Quote: ... the supernatant fraction was incubated with 2 ml Ni-NTA His-Pur resin (Thermo Fisher, PI88222) for 1 h ...