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Citations for Thermo Fisher :
2301 - 2350 of 10000+ citations for IL 4 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: Plasmid CassetteAv2_pBAD contains the CRISPR03 array cloned into the pBAD/Myc–His B backbone (Life Technologies).12 It was used to prepare CRISPR DNA substrates by PCR with primers MMB1Lead40-5 and MMB1crisp3-r1 using Phusion High-Fidelity DNA polymerase according to the manufacturer’s protocol (New England Biolabs or ThermoFisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The inserts were released by Bam-HI and Eco-RI digestion and ligated to pENTR4 (Invitrogen; previously digested with the same restriction enzymes) ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Biophysics 2022Quote: ... The His tagged RBD construct was purified from the culture supernatant of Expi293 cells (Thermo Fisher) that were transfected with the plasmid DNA using FectoPRO (Polyplus Inc.) ...
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bioRxiv - Plant Biology 2023Quote: ... His-tagged AtKu70/80 was isolated from the supernatant using HisPur Ni-NTA resins (Thermo Fisher) and dialysed in 20 mM HEPES pH7.5 ...
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bioRxiv - Microbiology 2023Quote: ... brucei rRAP1-His at 10 µM was incubated with 10 mM SYPRO Orange dye (ThermoFisher Scientific), filtered before use with a 0.22 μm sterile filter (Fisher scientific) ...
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bioRxiv - Microbiology 2023Quote: ... Cloning strategy for generating codon optimized SARS CoV-2 ORF7a in pCDNA-V5/His TOPO (Invitrogen) has been described previously (13) ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Immunology 2023Quote: ... + 10% (v/v) HI-FBS + 100 U/ml penicillin-streptomycin (pen-strep, ThermoFisher Gibco™ #15140122) at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... before preparation for sequencing with using Ion PGMTM Hi-QTM View Sequencing Kit (Thermo Fisher Scientific). The library was added to an Ion 318TM Chip Kit v2 BC (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... The digestion cocktail for RNase HI-negative samples was further supplemented with 120 U RNaseOUT (Invitrogen), while the digestion cocktail for RNase HI-positive samples were supplemented with 10 U recombinant Escherichia coli RNase HI (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... His-tagged proteins were purified using 0.2 mL HisPur™ Ni-NTA Spin Columns from ThermoFisher according to the manufacturer’s protocol with the exception of carrying out 4 washing steps ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was incubated with an anti-His Rabbit primary antibody (Thermo Scientific, Massachusetts, United States) (1:1000) ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with protease and phosphatase inhibitor (PPI; Thermo Scientific, Rockford, IL, USA) and EDTA (Thermo Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... and L-glutamine (0.292 mg/mL) (Thermo Fisher Scientific, Rockford, IL, USA). The single cell suspension solution was filtered through 70-micron filter retaining tissue debris on filter ...
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bioRxiv - Immunology 2021Quote: Human interleukin two (IL-2) Ready-SET Go! ELISA kit (eBioscience/Invitrogen) and Nunc MaxiSorp 96-well plates (Thermo Fisher ...
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bioRxiv - Molecular Biology 2020Quote: ... Acquired spectra were processed using LipidSearch software v4.1 (Thermo Scientific, Rockford, IL) with the selection of following classes of lipids ...
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bioRxiv - Neuroscience 2020Quote: ... A BCA Protein Assay kit (Pierce, Thermo Fisher Scientific, Rockford, IL, USA) was used for retinal tissue ...
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bioRxiv - Cancer Biology 2020Quote: ... or SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific, Rockford, IL; 34095) and imaged using Amersham Imager 600 (GE ...
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bioRxiv - Bioengineering 2020Quote: ... Detection was performed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). All primary antibodies against phosphorylated proteins were obtained from Cell Signaling Technologies (Beverly ...
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bioRxiv - Biochemistry 2020Quote: ... GluC and Lys C protease were purchased from Thermo Scientific (Rockford, IL). Ammonium formate ...
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bioRxiv - Microbiology 2020Quote: ... and 20 U/ml recombinant interleukin-2 (IL-2; Thermo Fisher Scientific) at 37° C ...
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bioRxiv - Microbiology 2021Quote: ... all tissue samples were homogenized in TRIzol reagent (Invitrogen, Rockford, IL, USA) and then centrifuged to eliminate any sort of exces tissue particles and debris ...
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bioRxiv - Microbiology 2021Quote: ... Membranes were developed with SuperSignal West Dura Substrate (Thermo Scientific, Rockford, IL).
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bioRxiv - Microbiology 2020Quote: ... treated with the ECL Western Blotting Substrate (Thermo Scientific, Rockford, IL, USA) and visualized on X-ray film ...
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bioRxiv - Cell Biology 2021Quote: ... the Super Signal West Femto Chemiluminescent Substrate (Thermo Scientific, Rockford, IL, USA) was applied according the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... media was additionally supplemented with recombinant murine IL-12p70 (Thermo Fisher Scientific) and recombinant murine IL-18 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... PCR amplification was performed using TaqMan® master (Thermo Scientific, Rockford, IL) with the Roche LightCycler 480 Real-Time PCR system (AB7900HT) ...
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bioRxiv - Bioengineering 2021Quote: ... Detection was performed using an enhanced chemiluminescent substrate (Thermo Scientific, Rockford, IL). All primary antibodies against phosphorylated proteins were obtained from Cell Signaling Technologies (Beverly ...
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bioRxiv - Immunology 2022Quote: ... + 200 IU/mL IL-2 (NCI) + 0.08 mM 2-Mercaptoethanol (Gibco, 21985023) (NK-92 Media ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were developed with SuperSignal West Dura Substrate (Thermo Scientific, Rockford, IL) and imaged with a Bio-Rad Chemidoc MP imager (Bio-Rad).
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bioRxiv - Biochemistry 2019Quote: ... Alexa Fluor 594-conjugated secondary antibody (1:2000) (Invitrogen, Rockford, IL, USA) was added onto cells ...
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bioRxiv - Zoology 2020Quote: ... RPMI-1640 medium was sourced from Gibco (Gibco, Thermo Scientific, IL, USA). T4 DNA Ligase and JetFlex™ Genomic DNA Purification Kit was obtained from Invitrogen (Thermo Scientific ...
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bioRxiv - Developmental Biology 2019Quote: ... IL-7 20ng/ml (Miltenyi) and Lipids (hLDL) 50ng/ml (Life Technologies)63.
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bioRxiv - Cell Biology 2019Quote: ... siRNF167_2 separately using RNAimax (13778-075, Thermo Fisher Scientific, Rockford, IL, USA) in reduced serum-containing media (Opti-MEM ...
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bioRxiv - Cell Biology 2020Quote: ... antibodies to COPII (Sec23) and HS3ST5 from Thermo Scientific (Rockford, IL, USA) and antibody to HS2ST (N-term ...
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated proteins were detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Plant Biology 2019Quote: ... Biotinylated protein was detected with streptavidin-HRP (21124, Thermo Scientific, Rockford, IL).
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bioRxiv - Molecular Biology 2020Quote: ... determined using the Pierce™ BCA Protein Assay Kit (Thermo Scientific, IL).
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bioRxiv - Pathology 2021Quote: ... Knockdown experiments were performed using Silencer Select siRNA from Ambion STAT3 (4392420) and IL-6RAa (4390771) transfected using Lipofectamine RNAiMax Reagent (Invitrogen). All cells tested negative for mycoplasma contamination using MycoAlert (Lonza ...
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bioRxiv - Cell Biology 2020Quote: ... and reverse transcribed to cDNA using SuperScript VILO MasterMix (ThermoFisher, Rockford, IL). Samples were then subjected to qPCR using PowerUp SYBR Green Master Mix (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... RNA was extracted with TRIzol™ Reagent (ThermoFisher Scientific, Rockford IL, USA) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... filled with isopropanol (423830025, Acros Organics, Fisher Scientific, Hanover Park, IL, USA). Cells were then stored at -140 °C in liquid nitrogen (CryoPlus Storage Systems model 7402 ...
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bioRxiv - Immunology 2020Quote: ... and α-IL-2 (JES6-5H4, 25-7021-80, Thermo Fisher Scientific) for 2 hours on ice ...
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bioRxiv - Developmental Biology 2020Quote: ... and goat anti-rabbit Alexa-Fluor 488 or 546 (Invitrogen, Rockford, IL) were used as secondary antibodies at a concentration of 1:300 diluted in blocking solution for 2 hours at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... and three protease inhibitors: 1 mM AEBSF (ThermoFisher, Scientific, Rockford, IL, USA), 10 mM 1-10 phenanthroline (Sigma Aldrich Chimie SARL ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with protease inhibitor cocktail kit (Thermo Fisher Scientific, Rockford, IL, USA). Cell lysates were incubated overnight with 90 μL of Dynabeads® MyOne™ Streptavidin C1 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... IL-2 APC (MQ1-17H12, eBioscience) and IFN-g-PE (B27; Invitrogen). Other antibodies used were anti-murine CD19-eFluor450 (1D3 ...
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bioRxiv - Microbiology 2024Quote: ... the primers were treated with T4 Polynucleotide Kinase (Thermo Scientific, Rockford, IL) for 5’ phosphorylation according to the manufacturer’s instructions ...