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Citations for Thermo Fisher :
2651 - 2700 of 8618 citations for 7 oxo 7 phenyl heptanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in anE3 medium supplemented with 30 mg/ml 1-phenyl-2-thiourea (PTU, Acros Organics) from 24 hpf ...
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bioRxiv - Cell Biology 2020Quote: Quantitative PCR (qPCR) was performed on a QuantStudio 7 PCR system (Applied Biosystem) with Power SYBR® Green PCR Master Mix (Life Technologies, Carlsbad, CA, USA) using the following program ...
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bioRxiv - Genomics 2019Quote: 2 μl of TAF as well as conventional ChIP library was used for checking enrichment with various primer pairs (listed in Table 1) on Applied Biosystem ViiA™ 7 real time machine using SYBR green reagent (Life technologies, Cat No-4367659).
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bioRxiv - Molecular Biology 2021Quote: MCF-7 and MCF-10A cells were transiently transfected with the indicated siRNAs (Table S3) using Lipofectamine RNAi MAX (Thermo Fisher Scientific, Waltham, MA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: ... The final plasmids were assembled in a total volume of 20 µl using 100 or 200 ng of linearized ArEc-TDH3 or GR-306MP backbone with molar insert:plasmid ratio of 7:1 (116 or 281 ng of digested PCR product, respectively) and 1 µl of T4 DNA ligase (Thermo Fisher Scientific, catalog no. EL0011). The mixtures were incubated for 3 h at room temperature ...
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bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
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bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2021Quote: ... 1.7 μm particle size) (Waters Corporation, Milford, MA, USA) held at 50°C coupled to a Vanquish binary pump (Thermo Fisher Scientific, San Jose, CA). A gradient mobile phase was generated over 55 minutes with a flow rate of 250μL/min and was comprised of 10 mM ammonium formate in 40:60 (v/v ...
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bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were seeded (7 x 105 cells per 100 mm dish) in Dulbecco’s Modified Eagle Medium with 10% fetal bovine serum (Thermo Fisher Scientific Inc., Waltham, MA, USA) 1 day prior to transfection ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was carried out in Applied Biosystems ViiA 7 Real-Time PCR System with PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™ by Fisher Scientific). The relative transcript level was calculated by using the 2-ΔΔCT method which was normalized to ACTIN2 and PROTEIN PHOSPHATASE 2A SUBUNIT A3 s (PP2A3) ...
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bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies, Grand Island, NY, USA) with recombinant human GM-CSF (Miltenyi Biotech ...
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bioRxiv - Molecular Biology 2020Quote: ... Each group of mice was killed at 7 weeks of age, and their organs (blood, liver, heart and kidney) were removed and were soaked with RNALater (Thermo Fisher Scientific, Rockford, IL, USA) before being subjected to RNA extraction ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Cell Biology 2021Quote: ... THP-1 cells (1.5×105 cells/mL) were labeled with a fluorescent dye 2’,7’-bis(carboxyethyl)-5 (6)-carboxyfluorescein-AM (Thermo Fisher Scientific B1150; 1 mg/mL) in serum-free RPMI medium (Thermo Fisher Scientific 11875093 ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Physiology 2019Quote: ... equipped with a Acquity UPLC BEH 1.7 μM C-18 column (Waters, New York, NY) interfaced to a TSQ Quantum Access Max mass spectrometer (MS) (Thermo Fisher Scientific, Waltham, MA, USA). The isocratic mobile phase consisted of 80% acetonitrile and 20% water ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly isolated MNCs were washed and re-suspended in PBS and stained for 10 min at 37°C with 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) at a concentration of 5 µM (Thermo Fisher Scientific, Waltham, MA, USA). H2DCFDA is a non- fluorescent dye which is cleaved inside cells to 2’,7’-dichlorofluorescein (H2DCF) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were plated with antibiotic-free medium at a density of 7 × 106//T75 flask and transfected with Lipofectamine3000 (Thermo Fisher Sci, Waltham, MA, USA) using a total of 15 μg of lentiviral plasmids (described in the Supplement) ...
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bioRxiv - Cell Biology 2022Quote: ... HEK293T cells were plated with antibiotic-free medium at a density of 7 x 106//T75 flask and transfected with Lipofectamine3000 (Thermo Fisher Sci, Waltham, MA. USA) using a total of 15 μg of BEI lentiviral plasmids (NR-52520 ...
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bioRxiv - Systems Biology 2023Quote: ... 30 s at 72 °C and a final step of 7 min at 72 °C using a GeneAmp 2400 (Applied Biosystems, Foster City, CA, USA) thermal cycler ...
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bioRxiv - Cell Biology 2023Quote: ... at which the increase in the fluorescence signal started to be detected by the laser detector of the QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher Scientific, Waltham, MA, USA) as previously described (Bieche et al. ...
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bioRxiv - Neuroscience 2022Quote: ... 7 min), resuspended in plating medium (100 U/ml penicillin-100 μg/ml streptomycin, 1X GlutaMax supplement, 1X B27 (Gibco-Aldrich, Life Technologies, #17504-044) and 5 % FBS in neurobasal media (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR for quantification was run using an Illumina-compatible KAPA Library Quantification Kits (ROX Low) (cat# KK4873) on an Applied Biosystems ViiA 7 Real-Time qPCR / PCR Thermal Cycler System (Applied Biosystems, San Francisco, CA, USA) and was analyzed using QuantStudio software (RRID ...
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bioRxiv - Plant Biology 2023Quote: ... however we used nested primers (amiF and amiR, Supplemental table 7) to facilitate the cloning in the pENTR/D-TOPO vector (Thermo Fisher Waltham, Massachusetts, United States). Once cloned and sequenced ...
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bioRxiv - Microbiology 2023Quote: Total RNAs from BHK cells infected with JEV GI-SH 7 and JEV GIII-SH15 strains were extracted using TRIzol reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2021Quote: ... PDMPO [2-(4-pyridyl)-5-((4-(2-dimethylaminoethyl-amino-carbamoyl)methoxy)-phenyl)oxazole] (ThermoFisher Scientific, USA) was added to a final concentration of 330 µM ...
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bioRxiv - Microbiology 2023Quote: ... 1 μg/ml L-(tosylamido-2-phenyl ethyl) chloromethyl ketone (TPCK)-treated trypsin (Thermo Scientific Pierce), and 1x antibiotic-antimycotic (Corning) ...
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bioRxiv - Immunology 2023Quote: ... and 0.1% L-(tosylamindo-2-phenyl) ethyl chloromethyl ketone (TPCK)-treated trypsin (Affymetrix, Cleveland, OH, USA). Lastly ...
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bioRxiv - Plant Biology 2019Quote: ... benthamiana plants were inverted and all aboveground tissues were submerged into a Magenta™ Ga-7 Plant Culture Box (Fisher Scientific, Catalog No. 50-255-176) filled with the Agrobacterium suspension ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Bioengineering 2020Quote: ... 3 and 7) were chosen for staining the actin filaments of the cultured cells using Alexa Fluor™ 488 phalloidin (Thermo Fisher Scientific, A12379, the Netherlands), and nuclei using 4,6-diamideino-2-phenylindole dihydrochloride (DAPI ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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Characterization of pediatric porcine pulmonary valves as a model for tissue engineered heart valvesbioRxiv - Bioengineering 2023Quote: ... compound were sectioned with a cryotome to acquire 7 μm thick radial sections of PV tissue on Fisherbrand™ Superfrost™ Plus Microscope Slides (Thermo Fisher Scientific, cat #22037246). Slides were kept at -80°C prior to tissue staining ...
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bioRxiv - Cancer Biology 2024Quote: ... an additional 150 µL of a 1:1 mixture of NK-Xpander complete medium:OncoPro complete medium containing 500 U/mL rhIL-2 and 10 µM Invitrogen™ CellEvent™ Caspase-3/7 Red Detection Reagent (Thermo Fisher Scientific, cat# C10430) was added to each well ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were stained with 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH; Molecular Probes) at a final concentration of 100 μM and the cells were then immobilized on a 2% agarose pad made with sporulation buffer covered with a no.1.5 coverslip ...
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bioRxiv - Microbiology 2022Quote: ... Supernatants were aspirated and pellets were resuspended in 3-5 µL of 1X PBS containing 0.02 mM 1-(4-(trimethylamino) phenyl)-6-phenylhexa-1,3,5-triene (TMA-DPH)(Invitrogen).Cells were mounted on glass slides with polylysine-treated coverslips ...
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Plant Biology 2021Quote: ... qRT-PCR was carried out in Applied Biosystems ViiA 7 Real-Time PCR System with PowerUp™ SYBR™ Green Master Mix (Applied Biosystems™ by Fisher Scientific). The relative transcript level was calculated by using the 2-ΔΔCT method which was normalized to ACTIN2 and PROTEIN PHOSPHATASE 2A SUBUNIT A3 s (PP2A3) ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... was performed with Hieff™ qPCR SYBR® Green Master Mix (Yeasen, China) in a QuantStudio 6&7 Flex Real Time PCR System (Applied Biosystems, Life Technologies, Milan, Italy). The relative expression levels of IDH among the three body regions were calculated using the 2−ΔΔCt method [71] ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated for 7 days with secondaries (Goat anti-rabbit Alexa-568, Invitrogen A11036, or Goat anti-Rabbit Alexa-647, Invitrogen A-21245 1:500, 2 ml / sample) at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... The medium was changed every day and cells were passaged at 70%-80% confluency every 5-7 days using PBSCa-/Mg- (Life Technologies; Thermo Fisher Scientific, Inc., Waltham, MA, USA) supplemented with 0,5 mM UltraPureTM EDTA (Gibco ...
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bioRxiv - Bioengineering 2019Quote: ... and lithium phenyl-2,4,6-trimethylbenzoylphosphinate (LAP) photo-initiator were dissolved in Dulbecco’s phosphate-buffered saline (DPBS) (Life Technologies). A 200 μL mixture was prepared by mixing the corresponding amounts of stock solutions and DPBS to deliver two final solutions of 1 % (w/v ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 4969-Diamidino-2-phenyl-indole (DAPI) for the nuclear staining was purchased from Life Technologies (Grand Island, NY). Dimethylsulfoxide (DMSO ...
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bioRxiv - Cell Biology 2022Quote: ... For the bulk endocytosis experiments using the 4-[6-[4-(diethylamino)phenyl]-1,3,5-hexatrien-1-yl]-1-[3-(triethylammonio)propyl]-pyridiniumbromide dye (FM 4-64, Invitrogen), the cells were prepared as previously described [41] ...
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bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
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bioRxiv - Biochemistry 2023Quote: ... 5 mM disuccinimidyl suberate (DSS) or 2 mM tert-butyl disuccinimidyl phenyl phosphonate (tBu-PhoX)(Thermo Fisher Scientific) in DMSO was added to the solution and incubated for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Developmental Biology 2024Quote: ... Medium was replaced daily from 8 hpf and supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics) to inhibit melanogenesis ...
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bioRxiv - Developmental Biology 2024Quote: ... Larvae were then kept at 28.5°C with fresh E3 medium supplemented with 0.2 mM 1-phenyl-2-thiourea (10107703, Acros Organics). Around 3-4 hours after heat-shock ...