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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1 % dipalmitoyl PI(3)phosphate (PI(3)P diC16) (Life Technologies) and extruded to 400 nm using a polycarbonate filter and a hand extruder (Avanti Polar Lipids ...
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bioRxiv - Bioengineering 2023Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was performed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Bioengineering 2024Quote: ... and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC, Thermo Scientific, 22980) were added to the alginate solution in a molar ratio of 1 alginate:30 NHS:25 EDC ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed twice with cold FACS buffer (PBS + 2% FCS) and incubated in the dark at 4° C with an anti-TfR1 (CD71) antibody (clone OKT9; Thermo Fisher Scientific, Waltham, MA, USA). Cells were then washed with PBS and resuspended in FACS buffer ...
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bioRxiv - Plant Biology 2019Quote: ... proteins were hydrolyzed into their protein-bound amino acids in a sealed glass tube at 150 °C for 2 h with 500 μL methanesulfonic acid 4 M (Fisher Scientific; Hampton, New Hampshire, USA) after flushing out air with a gentle stream of nitrogen gas ...
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bioRxiv - Neuroscience 2020Quote: ... in TBST 1X at RT for 1 hour followed by incubation at 4°C overnight with primary antibodies (SYNGAP-1:2K, PA1-046, Thermofisher; b-Actin-1:5K, A2228, Sigma Aldrich). Membranes were washed thrice with TBST (0.1% Tween 20 ...
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bioRxiv - Microbiology 2022Quote: ... The F gene was PCR amplified using primers RSV F 5’ (5’GCAAGGATTCCTTCGTGAC3’) and RSV F 3’ (5’CACACCACGCCAGTAG3’) and Phusion High-Fidelity DNA Polymerase (Thermo Fisher Scientific). Sanger sequencing was performed by Elim Biosciences.
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bioRxiv - Molecular Biology 2023Quote: ... The 3×(CAC)2 and (CAC)2 RNAs were transcribed using T7 RNA polymerase (Thermofisher Scientific). A 10 μl binding reaction contains 10 nM RNA ...
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bioRxiv - Genomics 2020Quote: ... The purified RNA samples were quantified using Qubit RNA reagent kit on a Qubit fluorometer 3.0 (concentration range 3-7 ng/μl) (Invitrogen). RNA integrity (RINe ...
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bioRxiv - Genomics 2020Quote: ... media were replaced with 100 μL full melanoma media without phenol-red containing 4μM Caspase-3/7 activity dye (CellEvent, ThermoFisher). Plates where imaged using a Celigo Imaging Cytometer (Nexcelom ...
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Multi-Omic Analysis Reveals Disruption of Cholesterol Homeostasis by Cannabidiol in Human Cell LinesbioRxiv - Systems Biology 2021Quote: ... seeded with 2,000 cells/well and stained with Hoescht 33258 (1µg/mL) and CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) at a dilution of 1000x ...
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bioRxiv - Cell Biology 2022Quote: ... 2007) and DsRed2-Mito (Tanaka Bio) plasmids using a ratio of 7:3 using Lipofectamine LTX Plus (ThermoFisher, 15338100) for 5 hours ...
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bioRxiv - Biophysics 2022Quote: ... 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-ATTO647N (ATTO-lipid) and (iii) 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-Cyanine 7 (Cy7-lipid) were from Invitrogen, ATTO-TEC and Avanti Polar Lipids ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell proliferation was monitored every 24 or 72 hr for 3-7 consecutive days using CyQuant Reagent (Invitrogen, C35011) by measuring bottom-read fluorescence at 520 nm with excitation wavelength set at 480 nm using EnSight Multimode Plate Reader (PerkinElmer) ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells grown in 96-well plates were stained with CellEvent Caspase 3/7 green detection reagent (Thermo Scientific) according to manufacturer’s instructions and the fluorescence measured using a plate reader at 488 nm wavelength ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... One µL of cDNA was used in each qPCR reaction on a QuantStudio 5 (ThermoFisher Scientific) using SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
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bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
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bioRxiv - Cell Biology 2020Quote: ... 7- Amino-4-Chlormethylcumarin (CMAC) was part of the Yeast Vacuole Marker Sampler Kit (Y7531) from Thermo Fisher and staining was performed according to manufacturer recommendations ...
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bioRxiv - Bioengineering 2022Quote: ... were used for experiments in passage 4 to 7 and were cultivated in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... polyclonal CD8+ T cells were labelled with 20 μM CellTracker™Blue CMAC (7-amino-4-chloromethylcoumarin; Invitrogen) for 20-30 min at 37° C ...
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bioRxiv - Systems Biology 2022Quote: ... 4 × 108 cells per biological replicate were used after 7 days of blasticidin selection (10 mg/mL, Gibco), which was 9 days post-infection ...
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bioRxiv - Immunology 2024Quote: ... CD4 T cells previously labeled with CMAC cell tracer (7-amino-4-chloromethylcoumarin, CellTracker Blue CMAC Dye, Invitrogen) were added to the microscope (Nikon ECLIPSE Ti-TimeLapse microscopy) ...
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bioRxiv - Physiology 2024Quote: ... and stored at 4 °C overnight prior to storage at −80 °C following RNAlaterTM best practices (Life Technologies, 2011). Following this ...
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bioRxiv - Genetics 2021Quote: ... and hold at 4°C in a Veriti Thermal Cycler (Applied Biosystems). Agarose gel electrophoresis (1.5% Top Vision Low Melting Point Agarose gel (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight at 4°C with anti-GFP rabbit antibody (Invitrogen) diluted with 1% normal goat serum (NGS ...
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bioRxiv - Cell Biology 2021Quote: ... at 4 °C and 30 V overnight in Bolt transfer buffer (ThermoFisher). For staining ...
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bioRxiv - Microbiology 2021Quote: ... 4 °C) by dialysis (SnakeSkin Dialysis Tubing, 10k MWCO, Thermo Fisher Scientific). Protein was concentrated in 30k MWCO Amicon Ultra-15 centrifugal filters (MilliporeSigma ...
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bioRxiv - Biochemistry 2022Quote: ... 4*C ON incubation) in a black–flatted multi–well plate (NUNC). The plate was then blocked with a solution of 1% BSA in a TBS buffer for 2 h at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... cells were washed three times with 4°C phosphate-buffered saline (Gibco) and sulfosuccinimidyl-2-(biotinamido)ethyl-1,3-dithiopropionate-biotin (sulfo-NHS-SS-biotin ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... with 1X PBS at 4°C containing DAPI (Thermo Scientific™, #62248) at a final concentration of 1/3000 mg/ml ...
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bioRxiv - Cell Biology 2019Quote: RPE1 cells were lysed at 4°C in RIPA buffer (Thermo Scientific) supplemented with 1x protease inhibitor cocktail (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... overnight at 4°C using the heterobifunctional crosslinker Sulfo-SANPAH (Thermo Scientific, Waltham ...
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bioRxiv - Physiology 2021Quote: ... for 12-16 h at 4°C followed by 0.05% trypsin (Gibco) for 10-20 min at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... run at 4°C in a NuPAGE MES SDS running buffer (Invitrogen). All recombinant proteins were expressed in the BL21-Gold (DE3 ...
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bioRxiv - Immunology 2022Quote: ... adherent cultures were incubated with 4 °C 0.25% Trypsin-EDTA (ThermoFisher 25200072) for 15 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were mounted with fluoromount and stored at 4°C (ThermoFisher Scientific). Stacks were acquired with an epifluorescence microscope (Imager Z1 ...
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bioRxiv - Microbiology 2023Quote: ... Anti-β-actin (clone C-4, ICN691002) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were dissected in 4 ◦C 1X PBS (10173433, Fisher Scientific, UK) after a quick passage through EtOH (4146052 ...
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bioRxiv - Molecular Biology 2023Quote: ... and incubated at 4°C overnight: α-Tubulin (Invitrogen, cat no. A11126), final 100 ng/μL ...
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bioRxiv - Molecular Biology 2024Quote: ... at 4°C for 24 h in 1.5 mL DMEM (Gibco #11995065) with dispase II (2.1 U/mL ...
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bioRxiv - Cell Biology 2024Quote: ... one well was loaded with 5 µg of input mitochondria previously diluted in 5 µL of 2x LDS dye (Thermo Fisher Scientific, 84788) with 2.75 mM β-mercaptoethanol (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... and 5 ng/ml of fibroblast growth factor-2 (FGF-2) (all from Gibco). Cells were seeded at a density of 3200 cells/cm2 until they reached 90% confluency ...
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bioRxiv - Biophysics 2021Quote: Plates were incubated with the secondary antibody dilutions for one hour at 37 °C and developed with 1-Step™ Ultra TBM-ELISA (Thermo Scientific™, Waltham, MA, USA) reagent according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 × 106 HEK293F cells were transfected with one of these constructs using 293fectin Transfection Reagent (Thermo Fisher) or 293-Free (EMD Millipore) ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were then incubated with 4′,6-Diamidino-2-phenylindole (DAPI, 2 µM, Thermofisher) as a nuclear counterstain and Alexafluor 555 phalloidin (1:60 ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...