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Citations for Thermo Fisher :
2401 - 2450 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Neuroscience 2024Quote: ... denatured (70°C for 10min) protein samples (1 µg/µl) were loaded onto a NuPAGE 4-12% Bis-Tris Gel (Invitrogen) along with Chameleon 800 pre-stained protein ladder (LI-COR) ...
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bioRxiv - Pathology 2024Quote: ... at 4 °C overnight followed by an Alexa Fluor 594-conjugated secondary antibody (1:500) (A-11012, Thermo Fisher Scientific). Immunofluorescence staining images were acquired using the Pannoramic Scanner software (v3.0.3.139795 RTM) ...
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bioRxiv - Cancer Biology 2023Quote: ... and then incubated overnight at 4°C in a humidified chamber with anti-γ-H2AX (1:500, Invitrogen MA5-38225), anti-perilipin-2 (1:200 ...
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bioRxiv - Physiology 2024Quote: ... sections were incubated overnight at 4°C in primary antibody solution containing 10% goat serum and antibodies against laminin β1 (1:100; MA5-14657, Invitrogen) or laminin β1γ1 (1:200 ...
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bioRxiv - Biochemistry 2024Quote: ... The sample was dialysed in 1 L of purification buffer at 4°C using Snakeskin Dialysis Tubing (3.5 k MWCO, Thermo Fisher), followed by an overnight dialysis under the same conditions.
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bioRxiv - Microbiology 2024Quote: ... Proteins were purified at 4°C by the batch method over 1 mL Ni-NTA resin according to the manufacturer’s instructions (HisPur, Thermo Scientific). All buffers were 50 mM NaPO4 ...
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bioRxiv - Neuroscience 2023Quote: ... followed by an overnight incubation at 4 °C with 20 μg ml-1 laminin (Thermo Fisher Scientific, 23-017-015). After being thoroughly washed with PBS ...
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bioRxiv - Immunology 2024Quote: ... the membranes were probed overnight at 4°C with anti-mouse CASP-1 (Cat# 14-9832-82; Thermo-Fisher Scientific) or anti-mouse mature IL-1β (Cat# 63124S ...
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bioRxiv - Neuroscience 2023Quote: ... followed by a goat anti-rabbit Alexa Fluor 546 secondary antibody (1:1000; overnight at 4 °C; # A-11035, Invitrogen). Sections were mounted in a medium containing 50 % glycerol ...
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bioRxiv - Cell Biology 2024Quote: ... at 4°C o/n and then with 40 μL of pre-blocked (1 mg/ml BSA) Dynabeads protein A (ThermoFisher). Beads were sequentially washed with Wash buffer 1 (20 mM Tris HCl pH 8.1 ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were washed extensively in PBS and then incubated overnight at 4°C in blocking solution with the appropriate concentration of secondary antibodies diluted at 1:500 and with DAPI (Invitrogen) diluted at 1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... and incubated overnight at 4°C in a humified chamber with anti-AKAP12 antibody (Thermo Fisher #PA5-21759, 1:200) or anti-Ki67 (CST #9449 ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were blocked for 2hrs at RT in 5% milk powder and then incubated overnight (o/n) at 4°C with primary rabbit anti-GFP antibody (1:1,000; Molecular Probes). Membranes were washed 3x for 10min in 1X TBST ...
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bioRxiv - Neuroscience 2023Quote: ... in PBT then incubated for 24–48 hr at 4 °C in the primary antibody mouse anti-GFP (1:500 in blocking buffer, Ref# A11120, Invitrogen). The sample was washed in PBT for 3 hr at 25 °C then incubated in the secondary antibody AF488-conjugated goat-anti-mouse (1:500 in blocking buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... in blocking buffer overnight in 4°C and stained with secondary AlexaFluor633 donkey anti-goat antibodies (Invitrogen, #A21082; 1:200) for 1 hour at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... and D1-D4 (domains 1-4) with C-terminal 6x-His or StrepII tags were transiently transfected using the Expi293 expression system (ThermoFisher)7 ...
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The viral pellets were then concentrated at 70,000 x g for 1 hour at 4°C and gently resuspended in 140 ul of DPBS (Gibco; 14190144) and allowed to resuspend overnight at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were washed again in 0.5% Triton PBS and incubated for 2 h at room temperature or overnight at 4 °C in secondary antibody (donkey anti-goat 647, 1:1000, A-21447, ThermoFisher) in carrier solution ...
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bioRxiv - Neuroscience 2023Quote: ... rinsed 3X in 0.1% PBST and incubated in goat anti-mouse Alexa Flour 647 antibody overnight at 4°C (1:300, ThermoFisher A32728). Images were acquired using a Zeiss 880 Laser Scanning Confocal inverted microscope with a Plan-Apochromat 63x/1.4 oil DIC M27 lens ...
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bioRxiv - Biochemistry 2022Quote: ... diluted in PBS-T overnight at 4°C and detected with 1:2000 HRP-conjugated goat anti-chicken secondary antibody (#A16054, Invitrogen) diluted in PBS-T during 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were incubated overnight at 4°C with the following primary antibodies: TMEM163 (Thermofisher, cat # PA5-114329; 1:100), CD83 (Biolegend ...
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bioRxiv - Synthetic Biology 2023Quote: ... the membrane was incubated overnight at 4°C with primary antibody solution (1:1000 dilution for anti-TSG101 [Abcam, ab30871], anti-Calnexin [ThermoFisher, PA5-19169] and anti-Syntenin-1 [Origene ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were spun at 100,000xg for 1 hour at 4°C using Sorvall Discovery MX120SE Ultracentrifuge (Thermo Scientific; Waltham; MA) to isolate the cytosolic fraction ...
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RNA-binding protein YBX1 promotes Type H vessels dependent bone formation in an m5C-dependent mannerbioRxiv - Molecular Biology 2023Quote: ... the cells were counted and incubated for 45 min at 4°C with EMCN antibody (#2114518, Invitrogen, USA, 1:100) and CD31 antibody (#FAB6874G ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were incubated and cut depending on their molecular weight (KDa) and incubated with primary antibodies overnight at 4°C in a shaker (Cofilin Total 1:500 Invitrogen #MA517275 ...
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bioRxiv - Neuroscience 2023Quote: ... for 24 hours at 4°C followed by incubation with a biotinylated goat anti-mouse (1:200, ThermoFisher Scientific A28181) antibody for two hours at room temperature and then a streptavidin conjugated Alexa-Fluor 488 (1:200 ...
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bioRxiv - Developmental Biology 2023Quote: ... embryos were incubated overnight at 4°C with secondary antibodies conjugated with Alexa Fluor 633 (1:500; Goat anti-Rabbit Invitrogen A21070 ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Samples were incubated overnight at 4°C with 1:250 dilutions of the anti-rabbit Alexa Fluor 488 (A-11008, Invitrogen), anti-mouse F(ab ...
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bioRxiv - Biochemistry 2023Quote: ... at a final concentration 2.5 μg/mL for 1 h at 4 °C followed by Concanavalin A conjugated with Alexa Fluor 350 (Thermo Scientific) at final concentration 0.5 mg/mL for 3 h at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Thick slices were then incubated overnight at 4°C with streptavidin coupled to Alexa-568 (1/500, Invitrogen, S11226, RRID:AB_2315774) diluted in PBS containing 0.3% Triton X100 and 1% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... and stained by sequential incubation with primary antibodies diluted with PBS at 4°C overnight and with Alexa Fluor 488- or Alexa Fluor 555-conjugated secondary antibodies (1:450; ThermoFisher) or NeuroTrace500/525 (1:250 ...
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bioRxiv - Neuroscience 2023Quote: ... The lysates were incubated overnight at 4°C with a 1:100 dilution of anti-RyR2 antibody (C3-33, Invitrogen). Following the overnight incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... for at least 1hr and incubated overnight with primary antibodies at 4°C (anti-METTL3, ThermoFisher # 15073-1-AP; anti-RNGTT, ThermoFisher # 12430-1-AP ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated overnight at 4° C in a 1:1000 dilution of secondary antibody (Thermofisher goat anti-rabbit AF546, 11010) in PBT+ ...
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bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated 1 hour in blocking solution and incubated at 4 °C overnight with secondary antibodies conjugated with AlexaFluor (Molecular probes) diluted in blocking solution ...
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bioRxiv - Cell Biology 2024Quote: ... overnight at 4°C followed by corresponding Alexa Fluor 488- and Alexa Fluor 594-conjugated secondary antibodies (1:500; Invitrogen) or anti-αSMA-Cy3 antibody (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: ... and THP-1 cells were incubated with GZMK (50 nM) or C1s (50 nM) for 1 h on ice at 4 °C in HBSS with Ca2+ and Mg2+ (Gibco) containing 10 mM HEPES and 0.5% BSA (Millipore Sigma) ...
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bioRxiv - Immunology 2024Quote: ... The fibroblasts were incubated with primary antibody overnight at 4°C and then stained with the secondary antibody (all 1:1000, Alexa Fluor; Invitrogen) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... the sections were incubated in the same solution overnight at 4 °C with the primary antibodies anti-GFP (1:1000, Invitrogen) and anti-phospho-S6 Ribosomal protein (Ser236/236 ...
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bioRxiv - Molecular Biology 2024Quote: ... Larvae were thoroughly washed in PBS-Tx and incubated overnight at +4°C in secondary antibody (1:400 goat anti-chicken Alexa488 A-11039, ThermoFisher) in blocking buffer ...
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bioRxiv - Plant Biology 2024Quote: All fragments including the CENPC-k and CENPC motifs with and without DNA-binding regions (Supplementary Table 1-4) were amplified from the αKNL2 and CENP-C cDNA clones in pDONR221 (Invitrogen) vector using primers listed in Supplementary Table 5 ...
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bioRxiv - Developmental Biology 2024Quote: ... for two nights at 4°C and then with Alexa Fluor 555-conjugated Donkey anti-rat secondary antibody (1:50; Cat. No. A11077, Invitrogen). Light-sheet images were captured by LiTone XL Light-sheet Microscope (Light Innovation Technology Limited ...
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bioRxiv - Developmental Biology 2024Quote: ... Slices were incubated for 48h at 4°C with primary antibodies anti-GFP (A11122, Invitrogen, Thermo Fisher scientific, 1:500), anti-Robo3 human (AF3076 ...
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bioRxiv - Cancer Biology 2024Quote: ... and then were incubated overnight at 4 °C with Alexa Fluor 488-labeled secondary antibody (Fisher Scientific, A32723, 1:200), and mounted with DAPI (Vector Laboratories) ...
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bioRxiv - Bioengineering 2022Quote: ... and 0.02 mM 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) (Invitrogen). The reaction was allowed to proceed on a rotary incubator at room temperature for at least 4 hours ...
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bioRxiv - Cell Biology 2019Quote: ... and 200 mM 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDAC) (Invitrogen) in water for 1 hour at 60°C ...
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bioRxiv - Biophysics 2022Quote: ... 19 mg EDC (1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide) (Thermo Fisher), 11 mg sulfo-NHS (N-Hydroxysulfosuccinimide ...