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Citations for Thermo Fisher :
2251 - 2300 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 1:2 diluted whole cell extract in buffer C was added to Dynabeads Protein G (Thermo Fisher Scientific) pre-incubated with HEXIM1 antibody ...
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bioRxiv - Developmental Biology 2019Quote: ... one midgut and 7 µL of Schneider’s medium were placed in each well and topped with a coverslip (Fisher Scientific, #12-545-81). Midguts were imaged using an inverted Zeiss LSM 880 immediately after mounting.
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Cell Biology 2024Quote: Isolated CDX2mCherry cells (n=5) mCherry-negative cells (n=4) and hES cells (n=2 pooled) were centrifuged and 200uL Tri reagent (Thermo Fisher Scientific, USA) was added and incubated at room temperature for 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysis buffer (20 mM Tris pH 7.4, 150 mM NaCl, 5 mM MgCl2, 1% Triton X-100, 1 mM DTT, 25 U/ml DNase TURBO, Invitrogen cat#AM2238) had been added to the frozen monolayer ...
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bioRxiv - Cancer Biology 2024Quote: ... final concentration: 5 µM) and 4 µL of 1 mg/mL Hoechst 33342 dye (1:500 dilution) (Life technologies, Eugene, OR, USA) and resuspended thoroughly using a low binding pipette tip ...
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bioRxiv - Cell Biology 2020Quote: ... CDC20 was depleted using siRNA oligo #14 5’-CGGAAGACCUGCCGUUACA-3’ (ThermoFisher). siRNA oligos for PP2A-B55 and PP2A-B56 have been described (Hayward et al. ...
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bioRxiv - Cell Biology 2022Quote: ... 3-5 × 105 cells were settled on Polysine Slides (Thermo Fisher), fixed with 4% ...
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bioRxiv - Cancer Biology 2019Quote: ... human 36B4 RT 5′-CCCATTCTATCATCAACGGGTACAA-3′) using Superscript III (Thermo Fisher) at 55 °C for 1 h ...
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bioRxiv - Neuroscience 2020Quote: ... FIVNC-555p (5’-FAM-CATGGCCACATTAATAATGG CGCA -TAMRA-3’ (Applied Biosystems, CA). These reactions were performed with a Bio-Rad iCyclerTM iQ and analyzed using the manufacturer’s software ...
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bioRxiv - Cell Biology 2022Quote: ... DLP1-sense strand: 5’-UCCGUGAUGAGUAUGCUUUdTdT-3’ 31 (Ambion, Austin, TX, USA).
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bioRxiv - Cancer Biology 2019Quote: ... 5’-CAGGGGTGCAGCTTGATTTC-3’ 7500 Fast Real-Time PCR System (Applied Biosystems) using optimized conditions for SYBRGreen I dye system ...
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bioRxiv - Cell Biology 2020Quote: ... siRNA against MyoVa was obtained from Invitrogen (s9207, 5’ GUAUAGUCCUAGUAGCUA 3’) as this was shown to work well by Wu et al (2018) ...
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bioRxiv - Immunology 2020Quote: ... Reactions were run on a Quantstudio 3 or 5 instrument (ThermoFisher). Cycling conditions for Quantifast reagents were ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 5’- TTGGATCAGCTCAGACATATT-3’ or a nonspecific “scrambled” control (Invitrogen, United States). 72 hours after transfection ...
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bioRxiv - Neuroscience 2023Quote: ... or CRMP2 siRNA (5′ GTAAACTCCTTCCTCGTGT-3′; obtained from Thermo Fisher Scientific) using the 4D-Nucleofector (P3 Primary Cell Solution ...
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bioRxiv - Cell Biology 2023Quote: ... 3 days after transfection by 5 µl of lipofectamine 2000 (Invitrogen) with 2 µg of the plasmid and regularly re-sorted to maintain expression of myr/palm-mCherry.
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bioRxiv - Developmental Biology 2023Quote: ... cells were passaged every 3-5 days using TryplE (Gibco 12604054). Naïve and primed hPSCs were expanded and induced into different lineages in a 5% CO2 incubator at 5% O2 at 37C.
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bioRxiv - Molecular Biology 2021Quote: ... 4 μl of 5 × Vilo reaction mix (Life Technologies, 11754), and 2 μl of 2 × Superscript III enzyme blend (Life Technologies ...
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bioRxiv - Cell Biology 2022Quote: Neutrophils were loaded with Fluo-4/AM (5 μM, Invitrogen) in the presence of Pluronic F-127 (0.02% ...
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bioRxiv - Developmental Biology 2019Quote: DFOs loaded with fluo-4 AM (5 μM, Molecular Probes) was performed with a two-photon microscope (Moving Objective Microscope ...
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bioRxiv - Immunology 2023Quote: ... High 5 cells (BTI-Tn-5B1-4; Thermo Fisher Scientific) were infected with virus particles ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anticlaudin 7 (Invitrogen Cat#37-4800, 1:100 for IF); rabbit anti-ARPC2 (Abcam Cat#ab133315 ...
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bioRxiv - Neuroscience 2024Quote: ... AlexaFluor 568 goat anti mouse (Invitrogen, A11004, 7 RRID:AB_2534072, 1:2000), AlexaFluor 568 anti rabbit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... cells were resuspended at 1 × 10^7 in 90% FBS (ThermoFisher) 10% DMSO (Sigma Aldrich) ...
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bioRxiv - Immunology 2023Quote: ... Buffy coats were diluted 1:7 with Dulbecco’s PBS (Life Technologies). PBMC were obtained by density gradient centrifugation at 2000 rpm for 20 min at room temperature with 35 mL cell suspension stacked on 15 mL Biocoll separation solution (Biochrom) ...
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bioRxiv - Cancer Biology 2024Quote: Neuroblastoma BE(2)-C) cells were cultured in DME/F-12 1:1 (HyClone SH30023) supplemented with 10% Fetal Bovine Serum (Fisher Scientific FB12999102) and 1% Penicillin-Streptomycin (Lonza 17602E) ...
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bioRxiv - Cancer Biology 2023Quote: ... washed twice with PBS (1X) and incubated for 2 hours at 37°C with secondary detection antibodies (1:500) and phalloidin (1: 3000) (Invitrogen, Waltham, MA) for cytoskeleton staining ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Neuroscience 2021Quote: ... Brain halves were either drop-fixed in phosphate buffered 4% paraformaldehyde 4°C (Thermo Fisher Scientific, Waltham, MA) for 24 hours at for immunohistochemical staining or micro-dissected (cortex ...
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bioRxiv - Immunology 2024Quote: ... The tissues were fixed overnight at 4 °C in 4% methanol-free paraformaldehyde (ThermoFisher Scientific, cat no. U01H501), and then washed three times with 1xPBS for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... After fixing for 1h at 4°C in 4% PFA (prepared in PBS; Thermo Fisher Scientific, catalog 043368.9M), embryos were rinsed with PBS and then cryoprotected in 30% sucrose in 0.1M phosphate buffer overnight at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Cancer Biology 2024Quote: ... shRNA plasmids were cotransfected with the packaging and envelope plasmids using Lipofectamine 3000 at a ratio of 4:3:1 (#L3000001, Thermofisher) into LentiX cells ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The next day cells were transfected with a mixture of 4 ug plasmid DNA and a 1:3 ratio of PEI (1mg/mL) in Opti-Mem (Gibco). Forty-eight hours later ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were passaged every 3-4 days at a split ratio of 1:8 following dissociation with 0.5 mM EDTA (Invitrogen AM99260G) in PBS without MgCl2/CaCl2 (Sigma-Aldrich D8662) ...
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bioRxiv - Microbiology 2020Quote: ... for 2 h at 37 °C in opti-MEM (Gibco). Cells were washed 3x after transfection with media ...
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bioRxiv - Immunology 2021Quote: ... macrophages were preincubated for 5 min at 37 °C with 2.5 µM of Yo-Pro-1 iodide (Life Technologies) after galvanic current application or 1% triton X100 (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: COS-1 and HEK293T cells were cultured at 37 °C and 5% CO2 in Dulbecco’s Modified Eagle Medium (DMEM, Gibco) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2022Quote: ... CD4+ and CD8+ T-cells were stained with LysoTracker Red DND-99 (Invitrogen; 5 μM; 1 h; 37°C), fixed (3% PFA ...
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bioRxiv - Genomics 2021Quote: ... The cells were finally stained with calcofluor white (5 μg/mL for 1 h at 37°C; Invitrogen, USA). Alternatively ...
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bioRxiv - Microbiology 2020Quote: ... all grown at 37 °C and 5% CO2 and supplemented with 1% penicillin-streptomycin (Life Technologies Australia Pty. Ltd) A549 cells were grown in Ham’s F-12K (Kaighn’s ...
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bioRxiv - Immunology 2021Quote: ... Slices were maintained in a humidified incubator with 5% CO2 at 37°C with 1 ml MEM (Gibco, 31095209) containing 20% (v/v ...
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bioRxiv - Biophysics 2023Quote: ... HeLa and MDCK cells were cultured at 5% CO2 and 37°C in Dulbecco’s modified Eagle medium (DMEM, Capricorn) with 1% Penicillin Streptomycin (Gibco) and 10% fetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were placed in a thermocycler and when the temperature reached 55°C, 10 µL of enzyme mix (1 µL 10X ThermoPol Reaction buffer, 1 µL (5 units) Taq DNA polymerase (Thermo Fisher Scientific, 18038240) and 0.01 µL (0.025 units ...
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bioRxiv - Neuroscience 2024Quote: ... Germany) were grown at 37°C and 5% CO2 in a 1:1 mixture of Opti-MEM + Glutamax (Thermo Fisher Scientific; 51985-026) and DMEM (Dulbecco’s Modified Eagle Medium ...
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...