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Citations for Thermo Fisher :
2701 - 2750 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Sections were then blocked in serum before incubation with mouse monoclonal β-endorphin primary antibody (Abeam ab54205,1:200) for 24 h at 4°C and Alexa-fluor 488 chicken anti-mouse secondary antibody (Invitrogen, 1:400) for 24 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... washed three times in HBSS/1% BSA and incubated at 4 °C for 15 minutes in the dark with the following antibodies: LDLR (1/100; MAB CL 472413; Fisher scientific), CD36 (1/10 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The lysate was clarified by centrifugation (18 000g, 30 min, 4°C) and applied to a 1-ml Ni-NTA column (HisPur, ThermoFisher Scientific). After incubation at 4°C for 1 h the resin was washed with 50 ml of lysis buffer and eluted with 6 ml lysis buffer plus 250 mM imidazole ...
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bioRxiv - Microbiology 2021Quote: ... The sonicated cells were centrifuged at 14,000 rpm for 1 h at 4°C to remove the cell debris and the supernatant was incubated to Ni2+-NTA resin (Thermo Fisher) pre equilibrated with lysis buffer ...
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Observation of an α-synuclein liquid droplet state and its maturation into Lewy body-like assembliesbioRxiv - Molecular Biology 2020Quote: ... overnight at 4 °C and visualised with a fluorescent secondary antibody (goat-anti-mouse IgG Alexa 488-conjugated (1:5000, A11029, Life Technologies)) ...
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bioRxiv - Systems Biology 2020Quote: ... The following primary antibodies were diluted in the blocking solution and applied overnight at 4°C: mouse anti-TAU clone HT7 (1:500, Thermo Scientific), chicken anti-MAP2 (1:10,000 ...
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bioRxiv - Neuroscience 2022Quote: ... after which gels were transferred onto Amersham Protran 0.2 µm nitrocellulose membrane (Dutscher, 10600001) immersed at 4°C in NuPAGE Transfer Buffer (ThermoFisher, NP0006-1) for 90 minutes at 60V ...
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bioRxiv - Neuroscience 2022Quote: ... Incubation of sections from cerebral organoids in primary antibodies solution was performed overnight at 4°C and in secondary antibodies solution at room temperature for 1 hour (Alexa Fluor, Molecular Probes). The following primary antibodies were used ...
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bioRxiv - Biochemistry 2022Quote: ... which were subsequently vitrified at 4°C and 100% humidity for 4.5 s with a blotting force of -1 on a Vitrobot IV (Thermo Fisher Scientific) with standard Vitrobot filter paper (ø55/20 mm ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with antibodies (100 or 10 nM) for 1 h at 4°C and then stained Alexa647-conjugated goat anti-human IgG (Invitrogen, A21445) for 0.5 h at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... This was followed by blocking and incubation with appropriate primary antibodies overnight at 4 °C and species specific secondary antibodies [polyclonal Alexa fluor 488 goat anti-rabbit,1:1000 (A11070 of Invitrogen Biosciences), polyclonal Alexa fluor 555 rabbit anti-mouse,1:1000 (A21427 of Invitrogen Biosciences)] for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were then incubated for two days at 4°C with rabbit monoclonal anti-GFP antibody (1:2,000; Invitrogen, Waltham, USA) or rabbit polyclonal anti-c-fos antibody (1:2,000 ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were then washed twice with ice cold PBS (4° C) and subsequently transferred to cold PBS containing Ez-link Sulfo-NHS-LC-biotin (1 mg/ml; ThermoFisher Scientific) for 45 min ...
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bioRxiv - Molecular Biology 2020Quote: ... overnight at 4° C followed by goat anti-rabbit Alexa fluor 594 secondary antibody (1:400, Invitrogen, Carlsbad, CA, cat. A1102) incubation for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... acute hippocampal slices were labeled for 30 min at 4 °C with 1 mg/ml sulfo-NHS-SS biotin (ThermoFisher Scientific). Membranes were prepared and the biotinylated proteins were precipitated with streptavidin agarose resin (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... overnight at 4 °C and with horseradish peroxidase-conjugated rabbit anti-goat secondary antibody (1:3000; Thermo Fisher Scientific, Waltham, MA) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... primary antibodies were incubated for three nights at 4°C followed by incubation with donkey anti-mouse or anti-rabbit IgG secondary antibodies (1:1000, Life Technologies). Fluorescent images were acquired with an Olympus FV1000 at 2048 x 2048 pixel resolution using a 40x objective lens ...
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bioRxiv - Microbiology 2022Quote: ... Cell Signaling)] at 1:1,000 in blocking buffer overnight at 4°C with rocking and subsequently with HRP-conjugated goat α-rabbit or α-mouse secondary antibodies at 1:5,000 (ThermoFisher) in blocking buffer for 2 h at room temperature with rocking ...
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bioRxiv - Molecular Biology 2022Quote: ... spun at 4000 rpm for 15 minutes at 4 °C and the cell pellet was dissolved in 1 mL TRIzolR (Invitrogen, #15596026). An equal volume of ethanol was added to the mixture and total RNA was prepared using a Direct-zol™ ...
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bioRxiv - Developmental Biology 2019Quote: ... and sections were incubated overnight at 4°C with the following primary antibodies: rabbit anti-GFP (Invitrogen A-11122, 1:1000), mouse anti-Satb2 (Abcam ab51502 ...
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bioRxiv - Microbiology 2019Quote: ... waxworms were anesthetized at 4°C for ten minutes then ground in 1 mL of TRIzol™ (Invitrogen™, Carlsbad, CA) using the sterile 15 mL conical tissue grinder (VWR™ ...
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bioRxiv - Neuroscience 2019Quote: ... tissues were incubated overnight at 4°C in Alexa Fluor 488-conjugated goat anti-rabbit Ab (1:200) secondary antibody (Life Technologies) in PBS containing 10% normal sheep serum ...
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bioRxiv - Developmental Biology 2020Quote: ... overnight at 4°C and further stained with 1:200 Alexa Fluor 633 Goat anti-Rabbit IgG (H+L) Secondary Antibody (Invitrogen A21071) at a 20°C for 3 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated overnight at 4°C with an anti-CD63 primary antibody (1:20 dilution, Thermo Fisher Scientific, cat. # MA1-19281) in blocking buffer ...
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bioRxiv - Systems Biology 2020Quote: ... cells were stained at 4°C o/n in 50 µL of the 1st antibody diluent (rabbit anti-ZO-1, 61-7300, Thermo Fisher Scientific Inc. ...
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bioRxiv - Immunology 2021Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4◦C with 100 µl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Immunology 2020Quote: ... thoroughly rinsed twice with PBS and coated overnight at 4°C with 100 μl/well of 1 mg/ml biotinylated BSA (Thermo Fisher) in PBS ...
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bioRxiv - Microbiology 2020Quote: ... then 1 mL of a - 20°C pre-chilled precipitation solution was added (50% v/v acetone [A949-4; Fisher Scientific, Fair Lawn ...
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bioRxiv - Developmental Biology 2020Quote: ... Chromatin with antibody were rotated overnight at 4 °C before 1:40 protein A/G agarose beads (Thermo Fisher Scientific, #20423) were added and incubated for another 6 hrs ...
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bioRxiv - Cell Biology 2020Quote: ... 1:200) respectively at 4°C overnight and secondary antibody conjugated to Alexa Fluor 594 or Alexa Fluor 488 (Life Technologies) at room temperature for 1h ...
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bioRxiv - Physiology 2020Quote: ... Sections were incubated overnight (4 °C) with the respective primary antibodies: Anti-Ly6G rat monoclonal (1:100; Affymetrix, Santa Clara, CA) for neutrophils ...
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bioRxiv - Plant Biology 2019Quote: ... The slides were incubated overnight at 4°C with a primary antibody specific to GFP (1/200, Life Technologies, A-11122) then for 2 hours with goat-anti-rabbit-AlexaFluor488 secondary antibody (Life Technologies ...
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bioRxiv - Evolutionary Biology 2019Quote: ... and subsequently incubated with 1 mg/mL lysozyme at 4 °C for 30 min and further broken with French press (Thermo Scientific). The total lysate was centrifuged at 98,000 × g for 30 min at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with PBS and lysed at 4°C in ice-cold 1% triton lysis buffer supplemented with Pierce protease inhibitor tablet (Life Technologies) and phosphatase inhibitors ...
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bioRxiv - Cell Biology 2019Quote: ... then further cleared by centrifugation at 4°C and 25658 g for 1 h (Thermo Fisher Scientific, #A23-6×100 rotor). The supernatant was passed through a 0.22 µm filter (Corning #431097 ...
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bioRxiv - Cancer Biology 2021Quote: ... slides were incubated overnight in a humidified chamber at 4 °C in TBS-T with 1% Fatty acid free-BSA (Fisher Scientific) with anti-mouse/human antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... the following primary antibodies were diluted in 10 mL of 1.5% BSA in TBST containing 0.0005% sodium azide and incubated at 4 °C with the membranes overnight: α-GFP (1:2000, rabbit pAb, Life Technologies); α-Abl (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... embryos were washed five times in TBSN/BSA for one hr at 4°C whilst rotating and incubated with secondary antibodies overnight at 4°C at a dilution of 1:400 (Alexa Fluor 488 goat anti-mouse (Invitrogen, #A11001) and 1:400 Poly-HRP-conjugated secondary antibody from Tyramide SuperBoost Kits with Alexa Fluor 568 (ThermoScientific ...
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bioRxiv - Immunology 2021Quote: ... limbs were incubated in 5% sucrose (1 hour) and 30% sucrose (overnight) at 4°C and then embedded in OCT medium (Cat. # 23-730, Fisher Scientific). Transverse cryosections (12 μm ...
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bioRxiv - Immunology 2021Quote: ... overnight at 4°C followed by incubation for 1 hour with secondary goat anti–mouse IRDye 800 (Thermo Fisher Scientific, MA) or goat anti–rabbit Alexa Fluor 680 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... and effector cells were co-cultured for 4 hr at 37°C then fixed in 1% paraformaldehyde (Affymetrix, Santa Clara, CA). Cells were analyzed by flow cytometry (Symphony I/II ...
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bioRxiv - Neuroscience 2020Quote: ... 0.3% Triton X-100 in PBS) and incubated overnight (4 °C) with primary antibodies against TH (OPA1-04050 1:1000, Thermo Scientific) and HA (3F10 1:200 ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed for 1 h at 4°C using a FOXP3/Transcription Factor Staining Buffer Set (Invitrogen, for FOXP3 detection) or 15 min at room temperature using a 1.85% formaldehyde solution in PBS (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... Streptavidin pull-down was carried out overnight at 4°C in 1 ml volume containing 60 μl of streptavidin magnetic beads (Life Technologies), 1 % NP40 ...
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bioRxiv - Genomics 2022Quote: ... Sorted cells were centrifuged at 300g for 10 minutes at 4°C and resuspended in 1 ml of TRIzol (Invitrogen, 15596018). Spermatogonial RNA was purified according to the standard TRIzol protocol and contaminant genomic DNA was digested using the DNA-free kit (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Primary antibodies were incubated overnight at 4°C and included mouse anti-3OH-K (1:1000; MA1-16616, AB_568420, clone P3UI, Thermo Fisher Scientific) and mouse anti-β-Tubulin E7 (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... blocked again for 2h (10% CAS-Block / 90% PBT) and incubated overnight at 4°C with secondary antibody (1:250, Alexa-Fluor-594, ThermoFisher A32742) diluted in 100% CAS-Block ...
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bioRxiv - Genomics 2022Quote: ... and the resulting pellet was suspended in 0.85% NaCl (1.5 mL) and centrifuged (10,000 g, 1 min, 4°C) using Heraeus Primo R Centrifuge (Thermo Scientific, Schwerte, Germany). Pellets from the two tubes were dissolved in high salt buffer (1 mL ...
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bioRxiv - Neuroscience 2022Quote: ... Tissues were then incubated for 4 days at 37°C with a secondary antibody (donkey anti-rabbit AF488, Invitrogen, 1:1,000) in 1X PBS with 0.2% Tween 20 ...
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bioRxiv - Neuroscience 2024Quote: ... and incubated over-night at +4°C with donkey anti-sheep secondary antibody conjugated with Alexa Fluor 568 (1:1000, A-11011, Invitrogen, ThermoFisher Scientific) and Streptavidin conjugated with Alexa Fluor 488 (1:2000 ...