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Citations for Thermo Fisher :
2351 - 2400 of 10000+ citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2020Quote: ... embryos were incubated overnight at 4°C with goat anti-rabbit Alexa Fluor 488 secondary antibody (used 1:800 dilution, A27034 Invitrogen). Finally ...
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Biophysics 2022Quote: ... was dialyzed overnight in 1 L of 1 × TB at 4 °C in a Slida-A-lyzer mini dialysis unit with 3,000 MWCO (Thermo Fisher) to reduce the amount of glycerol in the sample ...
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bioRxiv - Cell Biology 2022Quote: ... cells were kept overnight at 4°C in IMDM medium supplemented with 10% FBS (HyClone) and 1% penicillin-streptomycin (Thermofisher). Staining was performed for 20min at room temperature (RT ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were fixed in 4% paraformaldehyde-PBS for 20 minutes at 37°C then stained in 1:2000 diluted Hoechst 33232 (ThermoFisher) for 10 mins.
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bioRxiv - Molecular Biology 2021Quote: ... overnight at 4 °C and further incubated with secondary antibodies (goat anti-rabbit Alexa fluoro 488, Invitrogen, 1:200 dilution). The nuclei were stained with DAPI (1:1000) ...
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bioRxiv - Biophysics 2019Quote: ... overnight at 4°C and after PBS washing were incubated with DAPI (1:1000, Thermo Fisher Scientific, Waltham, MA, USA) and Alexa Fluor 532 goat anti mouse (1:200 ...
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bioRxiv - Cell Biology 2019Quote: ... incubated with anti mTOR antibodies 1:200 overnight at 4°C and processed as above using anti-rabbit secondary antibody Alexa 555 (Invitrogen) at 1:400 in 3% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... in PBS containing 0.3% Triton X-100 (PBS-Tx) overnight at 4◦C followed by incubation with goat-anti-rat AlexaFluor647 (1:500; ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... staining was then performed in permeabilization buffer for 1 hour at 4°C before washing and resuspending cells in DPBS (Gibco). All flow cytometry data was acquired using Attune FxT flow cytometer and data was analysed using FlowJoTM software V10.
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... sections were incubated for 24 h at 4°C with Alexa Fluor488 chicken anti-goat IgG (1/1000, Molecular Probes) or Alexa Fluor488 goat anti-rabbit IgG (1/1000 ...
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bioRxiv - Developmental Biology 2021Quote: ... Slides were incubated with primary antibody overnight at 4°C followed by a 10min DAPI stain (1:30,000 at room temperature, Invitrogen, D1306) and 1h incubation with secondary antibodies including ...
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bioRxiv - Bioengineering 2021Quote: ... at 4°C for overnight and Alexa conjugated secondary antibodies (1:1000, Goat anti-rabbit IgG Alexa fluor 647, Invitrogen, A21245 ...
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bioRxiv - Neuroscience 2021Quote: A stable dispersion of SWCNTs was achieved based on previously published protocols28,43 through tip-sonication for 1 h at 30 % power at 4 °C (Fisher Scientific Model 120 Sonic Dismembrator ...
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bioRxiv - Cell Biology 2021Quote: ... overnight at 4°C and then with 1:250 goat anti-mouse Alexa Fluor 488 (A11008; Life Technologies, Waltham, MA) and 1:250 goat anti-rabbit Alexa Fluor 647 (A21244 ...
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bioRxiv - Plant Biology 2021Quote: ... a mouse anti-FLAG primary monoclonal antibody was used (anti-DYKDDDDK FG4R, 1 μg/mL overnight at 4 °C, Invitrogen), and horseradish peroxidase (HRP)-coupled goat anti-mouse IgG was used as a secondary antibody horseradish peroxidase (0.5 μg/mL for 1 hr at 21 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Wells were washed with PBS and stored overnight at 4°C in EL08.1D2 cell culture media supplemented with 1% P/S (Life Technologies).
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bioRxiv - Neuroscience 2022Quote: ... free-floating brain sections were incubated overnight at 4°C with primary anti-FSHR (1:200; catalog # PA5-50963, ThermoFisher), anti-NeuN (1:300 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were incubated overnight at 4°C with either mouse anti-mitochondria antibody (Thermo Fisher Scientific, 1:200, # MA5-12017); mouse anti-nuclei antibody (Merck ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed with PBS, and stained using anti-IgG AF647 (1:600 dilution in MACS, 30 min at 4°C) (ThermoFisher). Cells were then fixed using PFA 4% ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were incubated in the following secondary antibodies diluted to 1:1000 in ovary block overnight at 4°C: goat anti-rabbit IgG Alexa Fluor 633 (Invitrogen), goat anti-mouse IgG Alexa Fluor 488 (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were probed overnight at 4°C using the following primary antibody dilutions: rabbit anti-GFP (1:1000, Invitrogen #A11122), and mouse anti-brp (1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... the sections were labeled overnight at 4 °C with Alexa Fluor 488-labeled wheatgerm agglutinin (1:200; W11261, Thermo Fisher) in combination with a rabbit polyclonal primary antiserum ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... The plate is centrifuged for 1 minute at 1,000 rpm at 4°C and then inserted in the qRT-PCR machine (QuantStudio; Thermofisher scientific) and the run is parametrised ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated overnight at 4°C with gentle agitation in a chicken antiserum against GFP (1:1500, Invitrogen, A11120). After subsequent 3 x 10 min washes in PBST ...
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bioRxiv - Cancer Biology 2021Quote: ... antibodies were used at 1/200 concentration overnight at 4°C and secondary AF488-conjugated anti-mouse Ig (Life Technologies) at 1/500 for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and incubated overnight in a shaker protected from light at 4°C with respective detection reagents (DyLight 488-conjugated streptavidin at 1:400 dilution, Invitrogen #21832 ...
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bioRxiv - Immunology 2022Quote: ... and incubated for 30 min at 4°C the live/dead fixable aqua dead cell marker (1:1,000 in PBS; Life Technologies) before fixation ...
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bioRxiv - Biochemistry 2020Quote: ... in the same buffer at 4 °C overnight followed by incubation with Alexa Fluor 594 anti-mouse IgG Ab (1:1000, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Pathology 2019Quote: ... Muscle fibres were then incubated at 4°C overnight with rabbit polyclonal antibodies against synaptophysin (Syn, 1/500; ThermoFisher Scientific) and neurofilament 68 kDa (1/500 ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated overnight at 4°C in blocking-permeabilizing solution containing antibodies anti-PAX6 (mouse, 1:1000, Thermo Fisher Scientific ...
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bioRxiv - Physiology 2020Quote: ... EDL from both legs were dissected and maintained in 4°C Krebs–Henseleit buffer supplemented with 1×MEM amino acid mixture (Invitrogen) and 25mM glucose ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was removed after being centrifuged for 30 min at 20,000 × G and 1 mL of cold (4 °C) 80/20 methanol/water (Optima, LC/MS Grade, Fisher Scientific) was added to the remaining contents ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... the media was decanted and cells were incubated for 1 h at 37 °C with Fluo-4 Direct dye (Invitrogen) in FLIPR buffer (1× HBSS ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were incubated with primary antibodies overnight at 4°C using an antibody against V5 (mouse monoclonal R960-25; 1:1000; Invitrogen), spike (mouse monoclonal GTX632604 ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were washed a further three times in PBTw (PBS + 0.1% Tween) and incubated overnight whilst rotating at 4°C in 0.005 U/µl Alexa Fluor 594 phalloidin (Invitrogen, #A12381) in PBTw ...
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bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
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The MuSK-BMP pathway maintains myofiber size in slow muscle through regulation of Akt-mTOR signalingbioRxiv - Cell Biology 2022Quote: ... Serum was diluted 1:1000 in 1/10 block overnight at 4°C and detected using goat anti-human IgG Alexa Fluor 555 (Invitrogen) diluted 1:1000 and counterstained with AlexaFluor488-conjugated α-bungarotoxin (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Immunology 2022Quote: ... Cells were then incubated with anti-IgM antibody (1/100) (Jackson Immuno Research) for 30 min at 4°C and mounted in Fluoromount-G with DAPI (Invitrogen). Fluorescence was detected using an inverted confocal microscope ...
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bioRxiv - Molecular Biology 2023Quote: ... for 1hr before being incubated overnight at 4°C in and primary antibodies (1:1000 mouse anti-V5 Tag monoclonal Antibody (Invitrogen) and anti-Actin monoclonal antibody (Invitrogen ...
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bioRxiv - Neuroscience 2023Quote: ... the sections were washed 3 times for 20 minutes each time in 1x PBS/0.3% Triton X-100/10% NDS at room temperature and incubated overnight at 4°C with secondary antibodies (Thermo Fisher Scientific Cat# A-21206, RRID:AB_2535792, 1:500; Thermo Fisher Scientific Cat# A-21203 ...
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bioRxiv - Neuroscience 2022Quote: ... overnight at 4°C followed by incubation with donkey anti-goat Alexa Fluor 594 secondary antibodies (1:200, A-11058; Invitrogen) and rabbit anti GFP (1:100 ...
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bioRxiv - Neuroscience 2022Quote: ... they were incubated for 24 hours at 4 °C with Alexa Fluor 594-conjugated donkey anti-goat IgG (1:300, A-11058, Invitrogen), Cy3-conjugated goat anti-rabbit IgG (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... before being incubated 72hours at 4°C with the following primary antibodies: chicken anti-GFP (1:1000, Life Technologies, #A10262) and rabbit anti-FMRP (1:500 ...
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bioRxiv - Neuroscience 2022Quote: ... WAKO) overnight at 4 °C followed by detection with Alexa 488 conjugated anti-rabbit secondary antibody (1:1000, Life Technologies), and Alexa 594 conjugated anti-rat secondary antibody (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... Overnight incubations (4°C) of the sections were carried out with mouse monoclonal anti- proliferating cell nuclear antigen (PCNA) (1:200, Invitrogen). Control sections were incubated with a mouse IgG Isotype Control (1:200 ...