Labshake search
Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Co-immunoprecipitation experiments were then carried out using DynabeadsTM M-280 streptavidin (Thermo Fisher Scientific). To allow time for interaction ...
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bioRxiv - Genetics 2023Quote: Protein lysates were obtained by homogenizing tissues in M-PER lysis buffer (ThermoFisher; Cat# 78501) with 1x Complete Mini EDTA-free protease inhibitor cocktail tablets (Roche Applied Sciences ...
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bioRxiv - Biochemistry 2022Quote: ... 80,000 M-1·cm-1) against the protein concentration determined using Bradford protein assay (ThermoFisher).
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bioRxiv - Neuroscience 2023Quote: ... and reverse transcribed into cDNA using oligo dT primers and M-MLV Reverse Transcriptase (Invitrogen). Real-time PCR was performed on Quant Studio 6 Flex Real-time PCR system (Applied Biosystems ...
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bioRxiv - Immunology 2023Quote: ... followed by incubation with magnetic streptavidin beads (Dynabeads™ M-280 Streptavidin, Thermo Fisher Scientific) for 15 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... or 40 µl DiD solution (0.5 m/ml in 0.9% saline, D7757, Thermo Fisher Scientific) via PITI on two consecutive days ...
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bioRxiv - Biochemistry 2023Quote: ... The resulting extract was added to magnetic beads (Life Technologies, Dynabeads M-270 Epoxy, 14302D) coupled to rabbit IgG (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... Dried purified silk fibroin was dissolved in 9 M lithium bromide (LiBr, Acros organics, 199870025) solution in UPW at 55°C for 1 hour and dialyzed against UPW for 36 hours using SnakeSkin Dialysis tubing (molecular weight cut-off (MWCO) ...
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bioRxiv - Biophysics 2023Quote: ... 1 M NaCl was incubated with 100 μg succinimidyl ester of AlexaFluor-647 (ThermoFisher, A20006) in the dark for 1 h at 25°C with gentle shaking ...
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bioRxiv - Molecular Biology 2022Quote: ... for COMP-Ang1 IP were covalently coupled to M-270 Epoxy Dynabeads™ (Thermo Scientific), cat ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was generated from 1 μg of total RNA using M-MLV reverse-transcriptase (Invitrogen) and random hexamers (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... scaled up accordingly) M-270 streptavidin-coated Dynabeads (10 µL containing 100 µg, Thermo Fisher) were equilibrated and washed with 1xBW buffer (5 mM Tris-HCl pH 7.5 ...
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Essential roles of the ANKRD31-REC114 interaction in meiotic recombination and mouse spermatogenesisbioRxiv - Genetics 2023Quote: ... Fragments containing the biotinylated adapter were purified with Dynabeads™ M-280 streptavidin (Thermo Fisher). The end-repair reaction was done using the End-it DNA end-repair kit (Lucigen) ...
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bioRxiv - Bioengineering 2023Quote: ... we added 200 μL of M-PER lysis buffer (Thermo Fisher Scientific Inc., catalog #78503) on top of cells and incubated for 5 minutes at 180 rotations per minute (RPM ...
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bioRxiv - Microbiology 2023Quote: ... 1 M lithium acetate and 10 mg/mL denatured herring sperm DNA (Thermo Fisher Scientific) was added and incubated overnight shaking at 30 °C ...
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bioRxiv - Cell Biology 2023Quote: Mouse cDNA was synthesized by reverse transcription from kidney RNA by M-MLV (ThermoFisher 28025013) with random primers (Invitrogen 48190011) ...
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bioRxiv - Molecular Biology 2024Quote: ... protein was isolated using M-PER Mammalian Protein Extraction Reagent (Thermo Fisher Scientific Inc, 78501), and Halt Protease and Phosphatase Inhibitor Cocktail (Thermo Fisher Scientific Inc ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% 0.5 M EDTA solution and 1% protease and phosphatase inhibitor (Thermo Scientific). The lysate was centrifuged at 14,000 rpm for 15 minutes ...
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bioRxiv - Biochemistry 2019Quote: ... 5’-GMPS-pre-tRNAAsp was prepared by in vitro transcription in the presence of guanosine 5’-monothiophosphate (GMPS) and labeled by reaction with 5-iodoacetamido-fluorescein (5-IAF, Life Technologies) overnight at 37 °C to prepare Fl-pre-tRNA (37,38) ...
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bioRxiv - Microbiology 2019Quote: ... falciparum strain 3D7 parasites were grown under 5% O2 and 5% CO2 in RPMI-1640 media supplemented with 5 g/L Albumax II (Life Technologies), 2 g/L sodium bicarbonate ...
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bioRxiv - Cancer Biology 2020Quote: ... followed by sonication three times for 5 seconds with 5 seconds intervals at #5 (on dial) (Fisher Scientific 60 Sonic Dismembrator) on ice ...
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bioRxiv - Cell Biology 2020Quote: ... reverse: 5’-GGGGTCGGGAGGAACGG-3’ (Macrogen, South Korea) and beta-actin forward: 5’-CCTGGTCGGTTTGATGTT-3’ and reverse: 5’-GTGCGACGAAGACGA-3’ (Invitrogen, USA). Data analysis was made in CFX ManagerTM Software (BioRad ...
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bioRxiv - Evolutionary Biology 2022Quote: The purified RNA was used to amplify cDNA from the gUTRGFP template with the 5’ Cy-5 labelled pWP252fluor primer (5’ ATAACGGACTAGCCTTA 3’) using the standard Superscript III First-Strand Synthesis System (Thermo Fisher) with 3 µg of total RNA and elongating at 52.5°C for 60 min ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were cultured at 37 °C in a humidified incubator at 21% oxygen/5% CO2 or 5% O2/5% CO2 (HeraCell Tri-Gas, ThermoFisher Inc).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2022Quote: ... and L plasmids at a ratio of 4:2:2:2:1 using Lipofectamine 2000 (ThermoFisher Scientific). Cells were transfected in 6 well plates and subsequently transferred to T25 flasks with HEp2 cells until cytopathic effect (CPE ...
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bioRxiv - Cell Biology 2020Quote: ... 2 μM Rhod-2 AM (Thermo, R1244) and 2 μM Calcium green-1 AM (Life Technologies, C3011MP) was added to culture media and allowed to stain at 22 °C for 20 min prior to imaging (frozen Rhod-2 AM aliquots were used only when the DMSO suspension remained clear) ...
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bioRxiv - Molecular Biology 2021Quote: ... and FURA 2-AM (Fura-2-acetoxymethyl ester) from Invitrogen, CA ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μM oligodT (dT25) and 2 μM random hexamers (Invitrogen) or 50nM stem-loop specific primers (for miRNA expression analysis) ...
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bioRxiv - Bioengineering 2019Quote: ... 50 μM Gibco 2-Mercaptoethanol (2-ME) (Thermo Fisher, 21985023), 1x antibiotic-antimycotic (anti-anti ...
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bioRxiv - Cell Biology 2021Quote: ... A fluorescent derivative of 2-deoxyglucose (2-NBDG, Invitrogen, N13195) was added at a final concentration of 20 µM for a further 30 min incubation at 37 °C ...
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The structure of insulin granule core determines secretory capacity being reduced in type-2 diabetesbioRxiv - Physiology 2022Quote: ... Nucleobindin 2 and siRNA control #2 were purchased from ThermoFisher Scientific ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: ... 2 mM L-Gln and 50 μM 2-mercaptoethanol (Invitrogen).
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bioRxiv - Genetics 2022Quote: ... 2% B-27 and 1% N-2 supplements (Life Technologies) and supplements 20ng/ml BDNF ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were loaded with 2 μM Fura-2/AM (Invitrogen). Ratiometric Ca2+ imaging was performed at 340 and 380 nm in 0 or 2 mM Ca2+ Ringer’s solution using an IX81 microscope (Olympus ...
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bioRxiv - Cell Biology 2019Quote: ... 2 µg of DNA and 2 µl Plus reagent (Invitrogen) were added to 1 ml serum-free media in a centrifuge tube ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific)) supplemented with 4 ng/ml bFGF ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM L-glutamine and 2% penicillin/streptomycin (ThermoFisher Scientific). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were incubated with 2 μM Fura-2 (F1221, Invitrogen) at 37℃ for 30 min dispersed in Margo solution with 0.2‰ w/v Pluronic F-127 ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 µL FastAP Thermosensitive Alkaline Phosphatase (2 U) (Thermo Scientific) was added and the solution was incubated at 37°C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... containing 2% of 2-mercaptoethanol (Thermo scientific Cat. No: 125470100) and heated at 70°C for 10 mins before freezing them at – 80°C ...
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bioRxiv - Neuroscience 2024Quote: ... 2% B-27 and 1% of N-2 supplements (GIBCO), 1% L-glutamine ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.1 mM 2-mercaptoethanol (2-ME, Thermo Fisher Scientific), supplemented with 100 ng/mL Activin A (Nacalai Tesque) ...
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bioRxiv - Microbiology 2024Quote: ... IL-2 was measured by human IL-2 ELISA (Invitrogen) using the manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μg mL−1 puromycin (58-58-2, ≥98%, Invitrogen) and 100 μg mL−1 hygromycin (31282-04-9 ...
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bioRxiv - Immunology 2024Quote: ... followed by interleukin 2 (IL-2) (20 ng/mL, Gibco) stimulation for 5 days.
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bioRxiv - Bioengineering 2024Quote: ... 2-Nitrobenz Oxadiazol Amino Deoxyglucose (2-NBDG, Invitrogen, Cat# N13195) for 30 minutes (figure 4(a)) ...
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bioRxiv - Cell Biology 2023Quote: ... N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid (HEPES) (Gibco), Sodium Pyruvate (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... with 2 pulses of 950V lasting 2 ms by Invitrogen Neon Transfection system (Invitrogen) ...