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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 5 M TOLYL FURAN 2 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: Cultured GCs were collected and homogenized with the M-PER mammalian protein extraction reagent (Thermo Fisher) supplemented with complete protease inhibitors ...
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bioRxiv - Molecular Biology 2022Quote: Unheated and heated samples were lysed in mammalian protein extraction reagent (M-PER, Thermo Fisher Scientific) complemented with 1x protease and 1x phosphatase inhibitors (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... The remaining fraction (‘HKT1’ sample) was incubated with PBSb-equilibrated M-280 streptavidin-coated Dynabeads (Invitrogen), washed in PBSbt buffer (PBSb with 0.1% Triton X-100 ...
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bioRxiv - Cancer Biology 2022Quote: Cells or spheroids were incubated at 37°C for 30min with CellROX Green (5μM/R633, 10μM/P3) or Deep Red reagent (1.25μM/P3, 2.5μM/R633 and 5706**) (ThermoFisher, France). Fluorescence signal was then assessed by microscopy (CellROX Green ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated one hour with either 0.7 M hydroxylamine pH 7.4 (+NH2OH, HAM, Thermo Fisher Scientific), to cleave thioester bonds and remove palmitate groups ...
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bioRxiv - Neuroscience 2021Quote: ... and transcardially perfused with 25 ml of 0.125 M phosphate buffered saline (PBS, pH 7.4, Gibco) and 40 ml of 4% paraformaldehyde (PFA ...
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bioRxiv - Biochemistry 2020Quote: ... The pellets were washed once with 1× DPBS before lysis in M-PER buffer (Thermo Fisher) supplemented with 1× protease inhibitor cocktail (Roche) ...
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bioRxiv - Immunology 2021Quote: ... with program RNA_02 in M tubes (#130-096-335) in 1 ml of Trizol (Invitrogen, #15596018). The homogenates were centrifuged at 2000 g for 1 min at 4°C and the supernatant was collected ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleared lysates were incubated with 200 μL Dynabeads™M-280 tosylactivated (Invitrogen™, Cat#14203) coupled with rabbit IgG (15 mg of beads per samples ...
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bioRxiv - Immunology 2022Quote: ... followed by addition of 50 μl/well of a 3 M HCl solution (Thermo Fisher Scientific). Optical density (OD ...
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bioRxiv - Cell Biology 2022Quote: ... The eluted beads were then treated with 6 M guanidine hydrochloride (GdnHCl; [Thermo Fisher Scientific; 24115]) and incubated for 2 hrs at room temperature with mixing ...
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bioRxiv - Biochemistry 2020Quote: ... RNA was reverse transcribed using Moloney Murine Leukemia Virus (M-MuLV) reverse transcriptase (Thermo Fisher Scientific), in the presence of oligo (dT)15 primers ...
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Imaging of Morphological and Biochemical Hallmarks of Apoptosis with Optimized Optogenetic ActuatorsbioRxiv - Biochemistry 2019Quote: HeLa cells were lysed post-transfection with 200 µL of M-PER lysis buffer (Thermo Scientific) plus protease inhibitors ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested and lysates prepared using M-PER mammalian protein extraction reagent (Thermo Fisher Scientific), and protein concentration was measured using a bicinchoninic acid (BCA ...
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bioRxiv - Molecular Biology 2019Quote: Total protein was extracted from cells using M-PER Mammalian Protein Extraction Reagent (Thermo Scientific, USA) supplemented with protease inhibitor cocktail (Roche Applied Science ...
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bioRxiv - Molecular Biology 2019Quote: ... after washing and immobilization of 0.50mg/reaction of streptavidin-conjugated beads (M-280 – Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2019Quote: ... Fragmented DNA containing library adapters was bound to Dynabeads™ M-280 Streptavidin magnetic beads (Invitrogen) and washed with buffer to remove non-target fragments before an incubation step to release DNA from the beads ...
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bioRxiv - Molecular Biology 2019Quote: ... The supernatant was pre-cleared with protein A/G or Dynabeads M-280 Streptavidin beads (ThermoFisher) and immunoprecipitated for 3-12 h using 3 μg antibodies and 40 μl protein A/G or Dynabeads M-280 Streptavidin beads ...
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bioRxiv - Plant Biology 2019Quote: ... Elute was incubated with 100 μL Dynabead M-280 Streptavidin beads (Thermo Fisher Scientific, Waltham, MA) at 4 °C for 8 hours ...
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bioRxiv - Biochemistry 2020Quote: ... 7 M urea polyacrylamide gel electrophoresis and stained with SYBR Gold (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Microbiology 2020Quote: ... and washed with 0.5 M Arginine in Dulbecco’s PBS pH 7.4 (DPBS: Thermo Fisher, Waltham, MA) as described previously (42 ...
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bioRxiv - Molecular Biology 2021Quote: ... the MagMAX Cell-Free DNA Extraction (kit M; cat# A29319, Thermo Fisher Scientific, Waltham, MA, USA), and the QIAamp Circulating Nucleic Acid kit (kit Q ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysis was achieved via the M-PER™ Mammalian Protein Extraction Reagent (78503, Thermo Scientific), which was supplemented with Halt™ Protease Inhibitor Cocktail (87785 ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was generated by retrotranscription of 1g of total RNA using M-MLV reverse transcriptase (Invitrogen) and random primers (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Alcian blue solution containing 20 μL of 18 M sulfuric acid (Acros Organics, 7664-93-9), 1 mL of 8 M GuHCl ...
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bioRxiv - Systems Biology 2019Quote: ... normally differentiated or Paneth enriched organoid pellets were lysed in m-PER lysis buffer (ThermoFisher Scientific) containing protease inhibitor cocktail (Roche) ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... CA) equipped with a TR-FFAP column (50 m × 0.32 mm × 0.5 μm, Thermo Fisher Scientific). The samples were heated for 20 min at 35°C and the headspace needle and transfer line were set to 90 and 120°C ...
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bioRxiv - Genetics 2020Quote: ... The DNAse-treated RNA was then reverse transcribed using the M-MLV Reverse Transcriptase (Invitrogen #28025013) in a reaction volume consisting of 1ug of RNA ...
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bioRxiv - Genetics 2020Quote: ... Pulldowns were performed with 0.6-0.7mg of nuclear extracts using M-280 paramagnetic streptavidin beads (Invitrogen) as previously described23.
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was synthesized using an M-MLV reverse transcriptase-based cDNA first-strand synthesis kit (Invitrogen). qRT-PCR was performed using the SYBR® Green CR Master Mix Kit (Applied Biosystems ...
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bioRxiv - Immunology 2021Quote: ... Complementary DNA (cDNA) was generated using RevertAid H Minus M-MulV reverse transcriptase (Thermo Fisher Scientific). Expression levels were determined by quantitative real-time PCR on a Rotorgene 3000 (Qiagen Instruments ...
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bioRxiv - Biochemistry 2021Quote: ... the supernatant was removed and incubated with 8 M guanidine hydrochloride (Thermo Fisher Scientific, Waltham, MA) for 2 hrs to denature any aggregates ...
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bioRxiv - Microbiology 2021Quote: ... 40 U of Protector RNase inhibitor and 200 U of M-MLV reverse transcriptase (Invitrogen™), following the protocol described by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... or with siICMT (Dharmacon Smartpool siGenome #M-005209-01-0010) (Table S1) using lipofectamine RNAimax (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: 400 pmol of biotinylated RNA was bound to 100 µl Dynabeads® M-280 Strepavidin (Invitrogen) in RNA-binding buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2022Quote: ... 1/10 volume of 3 M sodium acetate and 1 μL glycogen 10 mg/mL (Thermofisher). Pellet obtained by centrifugation 15000 x g ...
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bioRxiv - Cancer Biology 2022Quote: ... 500ng of total RNA from each sample were reverse-transcribed using M-MLV Reverse Transcriptase (Invitrogen) and random hexamers ...
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bioRxiv - Genetics 2022Quote: ... The lysate was precleared by incubating with 15 μL dynabeads M-280 straptavidin (Invitrogen by ThermoFisher), pre-equilibrated with 1 mL lysis buffer [50 mM Hepes pH 7.5 ...
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bioRxiv - Genetics 2022Quote: ... The lysate was precleared by incubating with 15 μL dynabeads M-280 straptavidin (Invitrogen by ThermoFisher), pre-equilibrated with 1 mL lysis buffer [50 mM Hepes pH 7.5 ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated by adding 0.1 volumes of 3 M sodium acetate (Invitrogen/Thermo Fisher Scientific), 20 μg of glycogen (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was precipitated by adding 0.1 volumes of 3 M sodium acetate (Invitrogen/Thermo Fisher Scientific), 20 μg of glycogen (Invitrogen/Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were resuspended in 500 μL M-PER (mammalian protein extraction reagent) lysis buffer (Thermo Scientific). Lysates were incubated on ice for 30 minutes with gentle agitation every 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Total RNA was retrotranscribed with random primer and M-MLV Reverse Transcriptase (Thermo Fisher Scientific, 28025013). qPCR analysis was carried out in a QuantStudio 6 Flex RealTime PCR System (Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: Protein extracts from bone were prepared in M-PER buffer (cat#78501, Thermo Fisher Scientific, USA) containing phosphatase and proteinase inhibitors (cat#78442 ...
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bioRxiv - Biochemistry 2022Quote: ... EVD68 2C NΔ39: εmax = 20,860 M-1·cm-1) with NanoDrop One (Thermo Fisher Scientific Inc.). The ionic strength of the dialyzed protein solution was confirmed with a conductivity meter ...
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bioRxiv - Microbiology 2022Quote: ... the cells were lysed in mammalian protein extraction reagent (M-PER; Cat# 78503, Thermo Fisher Scientific) supplemented with Halt protease inhibitor cocktail (Cat# 877886 ...
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bioRxiv - Neuroscience 2022Quote: ... and oncostatin M (OSM) was performed in an ABI7500 Real-Time PCR System (Thermo Fisher Scientific) in combination with TaqMan® chemistry ...
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bioRxiv - Plant Biology 2022Quote: ... for 30 min at 37°C and transcribed using the M-MLV-Reverse Transcriptase (ThermoFisher Scientific) with random hexamer primers according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The filtrate was diluted two times the volume with buffer UA (8 M urea [Acros Organics, CAS No ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were alkalised with 1 M TEAB prior to labelling with TMTpro 18-plex (ThermoFisher) according to manufacturer instructions ...