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Citations for Thermo Fisher :
2451 - 2500 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... All experiments were performed between passages 4 to 6 where cells were dissociated using TrypLE express (Life technologies), a gentle animal origin-free recombinant enzyme and seeded at a cell density of 5000 cells/cm2.
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bioRxiv - Cancer Biology 2020Quote: ... mitochondria were purified from 4-6 × 107 cells using mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
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bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 0.5:1:0.1 (4 μg/6-well dishes) using Lipofectamine LTX PLUS (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Cell Biology 2023Quote: Cells cultured in 6 well plates were washed with PBS and fixed with 4% Paraformaldehyde (11400580, Fisher Scientific) in PBS for 5min at room temperature (RT) ...
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bioRxiv - Biochemistry 2023Quote: ... samples were blotted at 100% humidity for 3 s (13°C, 0 s drain time, blot force -3 to +2) with a Vitrobot Mark IV (Thermo Fisher Scientific) and plunged into liquid ethane ...
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bioRxiv - Developmental Biology 2024Quote: ... followed by transfer to a positively charged nylon membrane and then crosslinked with EDC (1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide) at 60°C for 1–2 hours and prehybridized with ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen, cat # AM8670). The membrane was then hybridized with 50 pmol mL−1 Biotin-labeled Locked Nucleic Acid-modified DNA probes (designed and synthesized by Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Cell Biology 2023Quote: ... All drug treatments were performed for 3 – 5 hours prior to imaging with a final concentration of 5 nM of actinomycin D (Gibco, 11805-017) or 1 µM of BMH-21 (Sigma-Aldrich ...
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bioRxiv - Systems Biology 2023Quote: ... A 1 mL aliquot of 2:2:1 (v/v) mixture of ≥ 99.9% purity acetonitrile (Fisher Scientific; Cat.A998-4) ≥ 99.8% purity methanol (Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... The complex was eluted by rotating the tubes for 2 hours at 4 °C in elution buffer containing 4 mM SDA (NHS-diazirine, succinimidyl 4,4′-azipentanoate, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... 4% 2-Mercaptoethanol and Bromophenol Blue) and loaded on NuPAGE 4-12% Bis-Tris Gel (catalog #WG1402BOX; Thermo Fisher Scientific). Proteins were transferred on PVDF membrane (catalog #162-0177 ...
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bioRxiv - Microbiology 2022Quote: Bacteria in early exponential phase (OD600 0.2) were washed twice in phosphate buffered saline (PBS) and resuspended in eBioscience™ Cell Proliferation Dye eFluor™ 670 (eFluor, Invitrogen) at a final concentration of 1 μg/ml in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... Blood free perfused lungs were digested in Phosphate Buffered Saline (Mg and Ca free) with 2 mg/ml Collagenase Type I (Gibco Cat 17100017) and 50 units of DNase (Millipore Sigma Cat D5025) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The immunization emulsion consisted of syngeneic CNS antigen (1 part brain to 2 parts of spinal cord) in phosphate-buffered saline (Thermo Fisher Scientific) emulsified 1:1 in complete Freund’s adjuvant (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2020Quote: Real-time PCR was performed with the primers and probes for murine COX I gene (mtDNA) and glyceraldehyde-3-phosphate dehydrogenase (Gapdh, nuclear DNA [nDNA]) (Applied Biosystems, Invitrogen, Foster City, CA, USA) as described using ddCt method in a Step One Plus Real Time PCR System (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Microbiology 2024Quote: ... then fixed in 4% PFA and stained with 5 μg/ml DAPI and 5 μg/ml CellMask Deep Red (Invitrogen C10046). Plates were imaged using the Opera Phenix high-content screening system ...
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bioRxiv - Neuroscience 2022Quote: Wild-type mice received a single iontophoretic injection of each anterograde tracer: biotinylated dextran amine (BDA; 10 kDa; 5% solution in 0.125 M phosphate buffer, pH 7.4; Thermo Fisher Scientific, Cat. No. D1956) or Phaseolus vulgaris leucoagglutinin (PHA-L ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed twice with phosphate-buffered saline for 5 minutes and incubated with Alexa Fluor 555-tagged donkey-anti-goat (Molecular Probes, 1:1000) and Alexa Fluor 647-tagged donkey-anti-rabbit (Molecular Probes ...
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bioRxiv - Microbiology 2023Quote: ... After lysis in breaking buffer (20 mM potassium phosphate, 5 mM EDTA, 1 mg mL−1 lysozyme, 1 Pierce Protease Inhibitor Tablet (Thermo Scientific, Rockford, IL), 1 mM DTT ...
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bioRxiv - Zoology 2023Quote: ... harvested parasites were washed three times in phosphate-buffered saline (PBS) before culturing at 37 °C with 5% CO2 in RPMI 1640 medium (Invitrogen, Carlsbad, CA, USA) containing ...
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bioRxiv - Evolutionary Biology 2022Quote: ... filters were centrifuged (2500 g for 5 min) and washed with nuclease-free phosphate-buffered saline (pH 7.4, ThermoFisher Scientific, Waltham, MA, USA). DNA was extracted using the DNeasy PowerSoil Pro Kit (Qiagen ...
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bioRxiv - Genomics 2024Quote: ... pelleted at 300 g for 5 min and washed once with cold 1× Dulbecco’s phosphate-buffered saline (PBS, Thermo Scientific, Gibco, Cat. #14190).
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bioRxiv - Microbiology 2024Quote: ... A direct colony suspension of a 0.5 McFarland standard was prepared using 5 mL of sterile 0.85% phosphate-buffered saline (1X PBS, Thermo Fisher Scientific, Waltham, VA, USA) and a colony from a blood agar plate incubated for 24 h at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2024Quote: ... diluted them 1:1000 in 200 µl phosphate-buffered-saline (PBS) and stained cells with the addition of 5 µl SYBR Green (Invitrogen, Thermo F. Scientific). We used a Novocyte 2000R (ACEA Biosciences ...
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Candida albicans enhance Staphylococcus aureus virulence by progressive generation of new phenotypesbioRxiv - Microbiology 2024Quote: ... aureus were centrifuged at 4000 rpm (Labnet Spectrafuge™ 6C) for 5 min and washed twice with Phosphate Buffer Saline 1X (PBS) (Fisher Scientific S.L.). Suspensions with a final optical density of λ = 550 nm (OD550 ...
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bioRxiv - Biochemistry 2023Quote: Inorganic phosphate (Pi) release from polymerizing F-actin was performed using an EnzChek phosphate assay kit (Thermo Fisher Scientific) according to the manufacturer’s protocol and under conditions identical to those described above for actin polymerization ...
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bioRxiv - Biochemistry 2024Quote: ... 50 μL reactions were prepared with 8 nM SWR1C mixed with 0.5 μM phosphate sensor (fluorophore-conjugated phosphate binding protein, ThermoFisher) in remodeling buffer ...
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bioRxiv - Immunology 2021Quote: ... mouse interleukin 6 (IL-6; ThermoFisher; 1:500). Sections were then stained for DAPI (Sigma ...
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bioRxiv - Biophysics 2021Quote: ... 5 % CO2 incubator for 5 hours before replacing the culture media with 2 mL B-DMEM (Thermofisher, cat. # 10566016) supplemented with 10 % FBS (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2021Quote: ... Samples were rinsed 2 times for 5 minutes with PBS++ and incubated with 5 drops of NucBlue (Life Technologies) for 10 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... A single bolus of 50μL 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected ...
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bioRxiv - Neuroscience 2023Quote: ... A 50μl bolus of 0.5% Texas-red dextran solution (70,000 MW, 5 mg/mL in 0.9% NaCl, t1/2 ∼ 25min, Termo Fisher Scientific) was injected at a constant rate of 30μl/sec using a syringe infusing pump (GenieTouch ...
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bioRxiv - Cell Biology 2023Quote: ... for 2–5 h at 37°C followed by a 5 min incubation in TrypLE express (Thermo Fisher Scientific) to generate small clumps of corneal endothelial cells ...
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bioRxiv - Pathology 2024Quote: ... according to manufacturer specifications in the presence of 10mM 5-Bromo-2’-Deoxyuridine 5’-Triphosphate (BrdUTP, Thermo Scientific, B21550), at 37°C in a dark humidified slide box for 90 minutes ...
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bioRxiv - Biochemistry 2023Quote: ... Restriction-digested shuttle vectors -1-6 (33) and -2-1 (14) for wc-1 and wc-2 respectively were recombined with PCR (Thermo Fisher Scientific, Catalog # F549S) products amplified with primers bearing point mutations of wc-1 or wc-2 ...
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from gastrocnemius muscles of experimental mice (n=4 BC-PDOX; n=4 BC-PDOX PIO; n=3 NSC-Con) using Trizol (ThermoFisher Scientific, Waltham, MA, USA) and established methods21 ...
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Development of monoclonal antibody-based blocking ELISA for detecting SARS-CoV-2 exposure in animalsbioRxiv - Microbiology 2023Quote: ... Purified proteins were dialyzed using 1x phosphate-buffered saline (PBS) solution under 4°C for three times and then concentrated by polyethylene glycol 8000 (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Neuroscience 2024Quote: Mice P55-74 were anesthetized with a 4% isoflurane-oxygen mixture (v/v) and perfused transcardially with phosphate-buffered saline (PBS, Gibco, Life Science Technologies, 70011044) followed by 4% paraformaldehyde (PFA ...
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bioRxiv - Cell Biology 2024Quote: ... were treated with either phosphate-buffered saline (PBS) for 4 hours or serum-free DMEM containing high glucose and 100 μM dexamethasone (DEX) (Acros organics, New Jersey, USA) for 24-48 hours ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were passaged every 2-3 days by washing with HBSS (Gibco, cat#14170), trypsinizing using 0.25% Trypsin-EDTA (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MMT) was obtained from Thermo Fisher. Crystal violet was purchased from VWR ...
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bioRxiv - Bioengineering 2020Quote: Nbs were diluted in a 2- or 3-fold series in Opti-MEM (Thermofisher). Each Nb dilution (110 μl ...
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bioRxiv - Physiology 2021Quote: ... cells were fed every 2-3 days with DMEM + 10% FBS (Thermo fisher Scientific) until differentiation was complete at day 10.
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bioRxiv - Immunology 2022Quote: ... The band are visualized with gel electrophoresis using 2-3% agarose (#16500500, Thermo Fisher) gel with SYBR Safe dye (#S33102 ...
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bioRxiv - Neuroscience 2022Quote: ... Approximately 2-3 sections were mounted on each Superfrost Plus slide (Thermo Fisher Scientific). All sections were air-dried at −20 ° C for 1 hour and afterwards stored at −80 ° C.
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bioRxiv - Molecular Biology 2024Quote: The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT, Thermo Scientific) assay was performed on cells seeded and treated in 96 plates for 72 hours with the free doxorubicin or doxo-EVs ...