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Citations for Thermo Fisher :
2651 - 2700 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and Coomassie blue G-250 (0.6% w/v final concentration) were added and samples were loaded on a 3-12% Bis-Tris NativePAGE gel (Invitrogen). BN-PAGE was performed on ice according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Correct image reconstruction and alignment for dual labeled images were assured using the X-Y coordinates of 3-6 100 nm fiducial beads (T7279, Thermofisher) as reference points to align signals with an affine/linear registration function using Localization Microscopy Analyzer (LAMA ...
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bioRxiv - Cell Biology 2024Quote: ... or from ventricular tissues of various mouse hearts was minced and placed into one well of 6-well non-coated plate and cultured in 3 ml MSC medium with Normocin (Invitrogen) and Gentamicin (GIBCO).28 The media was changed daily for 3 days and then every 2-3 days ...
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bioRxiv - Immunology 2022Quote: HeLa-CIITA cells were incubated in 6-well plates using 2-4.105 cells/well using OPTIMEM (Gibco) complemented with 10% FBS ...
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bioRxiv - Biochemistry 2022Quote: ... The quality of obtained microsomes was tested with 9-amino-6-chloro-2-methoxyacridine (ACMA; Invitrogen A1324) fluorescence quenching assays.
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bioRxiv - Microbiology 2024Quote: ... VeroE6 and MDCK ⍺2-6 cells were cultured in minimum essential medium (MEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2022Quote: ... before pooling desired fractions and concentrating with Pierce PES 2-6 mL concentrator columns (Thermo Fisher Scientific). Concentrated protein was aliquoted and flash frozen in liquid N2 before storage at -80°C.
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bioRxiv - Systems Biology 2022Quote: ... the minimal medium (DMEM with 2 mM L-glutamine) was supplemented with either IL6 (interleukin-6) (ThermoFisher), TNFα (tumor necrosis factor-alpha ...
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bioRxiv - Immunology 2024Quote: ... Control cohorts received 2 doses of: 104 PFU YF-S0*-vaccine [16] (n = 6) or MEM (Gibco) supplemented with 2% FCS (HyCloneTM ...
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bioRxiv - Neuroscience 2024Quote: HeLa cells (ATCC, #CCL-2) were plated in DMEM (Cellgro, #10-013CV) containing 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture plates ...
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bioRxiv - Cell Biology 2024Quote: Live worms were stained with 10 mM C-Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (Thermo Fisher Scientific) as previously described in49 ...
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bioRxiv - Cell Biology 2022Quote: ... 750,000 cells per well in a 6-well plate were transfected using Lipofectamine 2000 (5 μL; Thermo Fisher) as needed ...
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Microbiology 2020Quote: ... 1976) and maintained in 6% O2 and 5% CO2 and grown in sterile filtered RPMI 1640 media (Gibco) supplemented with 2 M HEPES ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Immunology 2024Quote: ... Monocytes were differentiated to macrophages by 6 days culture at 37 °C with 5% CO2 in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco) medium supplemented with 10% endotoxin-free heat-inactivated fetal bovine serum (Gibco) ...
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bioRxiv - Cell Biology 2020Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2-4 hrs at room temperature followed by immobilization with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Neuroscience 2024Quote: ... sham and uPA-CBP were anesthetized (4% isoflurane, 2 liters of oxygen) and transcardial perfusion fixed with 4% paraformaldehyde (PFA) (Cat #50-980-494, Fisher Scientific) in PBS 1x until stiff ...
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bioRxiv - Developmental Biology 2024Quote: ... with a Dionex IonPac AG11-HC-4 μm guard column (2 mm i.d × 50 mm, 4 μm particle size, Thermo Fisher Scientific) and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm ...
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bioRxiv - Developmental Biology 2024Quote: ... and a Dionex IonPac AS11-HC-4 μm column (2 mm i.d × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... co-transfection of pX-CRISPR and qTAG repair plasmids was performed at a 1:1 ratio using 4 μg of total DNA (2 μg cut + 2 μg repair) in Opti-MEM Reduced Serum Medium (31985070, Gibco) with Lipofectamine 3000 (L3000008 ...
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bioRxiv - Neuroscience 2024Quote: Intracellular calcium imaging was carried out on isolated taste bud cells loaded with 4 µM of the ratiometric calcium indicator Fura-2-acetoxymethyl ester (Fura-2 AM, Invitrogen) in Tyrode’s with 0.05% pluronic acid F-127 (Invitrogen ...
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bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Microbiology 2020Quote: The lipophilic fluorescent membrane probe 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH) (Themo Fisher Scientific) was used to assess EV associated lipids ...
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bioRxiv - Biochemistry 2020Quote: ... 7.5 μl of 6 μM samples were separated by SDS-PAGE (NuPAGE 4-12% Bis-Tris Gel, Life Technologies) or native gels (NativePAGE 4-16% Bis-Tris Gel ...
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bioRxiv - Microbiology 2020Quote: 4 μl of purified spores (6×107 spores/ml) were mixed with 40 μl of NucBlue (Invitrogen, catalog #R37605) and incubated at 25°C for 40 min ...
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bioRxiv - Genomics 2020Quote: ... standards (3 x 10μl) and samples (6 x ∼18μl) were loaded on a 4-12% Bis-Tris Nu-PAGE (Life Technologies) gel for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... Organ of Corti explants were isolated from C57BL/6 mice at postnatal day 4 in cold Leibovitz’s L-15 medium (cat. # 21083027, Gibco) and held in place by two glass fibers glued to the bottom of a Petri dish ...
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bioRxiv - Neuroscience 2024Quote: ... and passaged every 4–6 days using 0.5mM EDTA in Ca2+ and Mg2+-free 1x PBS (Thermo Fisher Scientific).
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bioRxiv - Biophysics 2022Quote: ... thoracic and lumbar DRGs were harvested from 4 to 6 week old MrprD-GFP mice and transferred to Hank’s buffered saline solution (HBSS) (Invitrogen). Ganglia were treated with collagenase (2 mg/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... and pGL4.74-Renilla luciferase plasmids in a ratio of 1:1:0.1 (4 μg/6-well dishes) by using Lipofectamine LTX PLUSTM (Invitrogen). The luciferase activity was assayed using the Dual-Luciferase Reporter Assay Kit (Promega) ...
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bioRxiv - Developmental Biology 2023Quote: ... Images were collected manually or automatically with 4-6 µs dwell time using Maps 3.11 software (Thermo Fisher Scientific) (Burel et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... HEPA1-6 were lysed in RIPA buffer and loaded on a 4-15% gradient polyacrylamide gel (Thermo Fisher, NP0335). Protein transfer was carried out using the iBlot system (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: The phosphatase activity of SHIP1 and SHIP2 was determined by measuring inorganic phosphate production using Phosphate Sensor (ThermoFisher Scientific, PV4407). Soluble inositol 1,3,4,5-tetraphosphate (I(1,3,4,5)P4 ...
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bioRxiv - Microbiology 2022Quote: ... was generated using an established low-pH model.7 Mid-log Mtb-lux was washed with PBS (1X) and transferred into PCB media: 1X phosphate-citrate buffer (PCB, composed of 0.2 M sodium phosphate (Fisher Scientific) with 0.1 M citric acid (Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: Michaelis-Menten single turnover kinetics assays derived from the protocol of 64 were performed using the Phosphate Sensor system to detect inorganic phosphate (Invitrogen; ThermoFisher Scientific catalogue number PV4407) ...
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bioRxiv - Cancer Biology 2024Quote: ... The amount of inorganic phosphate (PO43-) released due to the activity of TRAP1 was measured using the Malachite Green Phosphate Assay Kit (AMBION), by detecting the increase in absorbance at 650 nm with a plate reader.
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bioRxiv - Genetics 2020Quote: Real-time PCR was performed with the primers and probes for murine COX I gene (mtDNA) and glyceraldehyde-3-phosphate dehydrogenase (Gapdh, nuclear DNA [nDNA]) (Applied Biosystems, Invitrogen, Foster City, CA, USA) as described using ddCt method in a Step One Plus Real Time PCR System (Applied Biosystems ...
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bioRxiv - Neuroscience 2020Quote: ... were quantified in autaptic neuronal cultures using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43, Thermo Fisher Scientific, Waltham, MA, USA), similarly as in our previous report (Kawano et al. ...
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bioRxiv - Neuroscience 2023Quote: ... were visualized using N-(3-triethylammoniumpropyl)-4-(4-(dibutyl amino) styryl) pyridinium dibromide (FM1-43FX, a fixable analog of FM1-43 membrane stain, Thermo Fisher Scientific, Waltham, MA, USA). To stain the presynaptically active synapses of autaptic cultured neurons ...
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bioRxiv - Cell Biology 2021Quote: ... 6 EV samples (2 per cell type, 1 ZIKV and 1 control each) were fixed in 2% paraformaldehyde (PFA; Fisher Scientific, Cat #50-980-487) and processed as cited 59 ...
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bioRxiv - Neuroscience 2023Quote: ... and the lipid film obtained was hydrated for 30 min at 50 °C and 280 rpm with either 2 mL phosphate-buffered saline (PBS, Fisher Scientific, Hampton, US) for Lipo or 2 mL of 25 mg/mL dexamethasone (Sigma-Aldrich ...